From owner-recombination@net.bio.net Wed Nov 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Hussein abulreesh Newsgroups: bionet.molbio.recombination Subject: Question Date: 5 Nov 1998 14:43:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <71t9nc$8rp@net.bio.net> NNTP-Posting-Host: net.bio.net hi, Can anyone aswer this question "How does RNA primer differ from a typical RNA molecule copied from DNA?". Thanks, Hussein Abulreesh hussein@uqu.edu.sa From owner-recombination@net.bio.net Sun Nov 08 22:00:00 1998 Path: biosci!biosci!not-for-mail From: milot Newsgroups: bionet.molbio.recombination Subject: LOX P recombination system Date: 9 Nov 1998 06:32:50 -0800 Organization: Erasmus University Rotterdam Lines: 13 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <726uei$ltq@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, We now have a construct with two lox P sites side by side (same orientation). I'm wondering if anyone would know if a duplication or multimerization of the lox P would modify its efficiency in a CRE recombination system. Cheers, Eric Milot From owner-recombination@net.bio.net Wed Nov 11 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Carsten Hohoff" Newsgroups: bionet.molbio.recombination Subject: recombination potential,myogenic cells Date: 11 Nov 1998 16:07:08 -0800 Organization: Westfaelische Wilhelms-Universitaet Muenster, Germany Lines: 19 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <72d8rc$3om@net.bio.net> NNTP-Posting-Host: net.bio.net Dear researchers, does anyone have any information regarding the potential (if any) of murine C2C12 cells (myogenic cell line) or any other non-ES cell line for homologous recombination? I would appreciate any help! Best regards, Bertram Bleck bleckb@uni-muenster.de