From owner-recombination@net.bio.net Mon Jan 18 22:00:00 1999 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.recombination Subject: RECOM IS BACK ON-LINE!! Date: 19 Jan 1999 09:58:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Approved: genestructure@yahoo.com Distribution: world Message-ID: <782h3r$ok3@net.bio.net> NNTP-Posting-Host: net.bio.net RECOM is Back ON-LINE!! Thank you for your patience Graham Dellaire Moderator bionet.molbio.recombination ______________________________________________________ == Graham Dellaire, Ph.D. Moderator bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Thu Jan 21 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Ian Lyons Newsgroups: bionet.molbio.recombination Subject: Isogenic DNA Promoterless Gene Targeting Vectors Date: 22 Jan 1999 08:06:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <78a7m3$irh@net.bio.net> NNTP-Posting-Host: net.bio.net Over the last ten years it has become dogma that to get high efficiency homologous recombination (usually in ES cells) you need to have targeting constructs generated from isogenic DNA. This means that most folk in the gene knockout game use 129 ES cells and 129 targeting constructs. John Sedivy and collaborators have recently collated a heap of data that shows that in a whole range of human cell types, there is not an absolute requirement for isogenic constructs. (www.sciencemag.org, Jan 1 99) HOWEVER, all the work they consider uses promoterless constructs. ie , where the neo or hygro cassette is only functional if it integrates into a transcription unit. This must be a whole heap more efficient than constructs giving drug resistance when randomly integrated into the genome. What I'd like is some idea of how much more efficient promoterless targeting constructs are then standard replacement targeting constructs: as measured by (HR events)/ (total resistant cells). Can anyone provide any data points? When I've collated any input, I'll send you a summary. Thanks Ian Lyons Biochemistry, Uni of Adelaide. ilyons@biochem.adelaide.edu (ADD .au, bcs that's where I am) > > == Graham Dellaire, Ph.D. Moderator bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Wed Jan 27 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Patricia Ayoubi Newsgroups: bionet.molbio.recombination Subject: Postdoctoral Position Date: 28 Jan 1999 13:13:08 -0800 Organization: Oklahoma State University Lines: 44 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <78qjt4$h8n@net.bio.net> Reply-To: ayoubi@mendel.microb.okstate.edu NNTP-Posting-Host: net.bio.net POSITION ANNOUNCEMENT > Postdoctoral Position. Department of Microbiology and Molecular Genetics > Oklahoma State University. Stillwater, Oklahoma. > > A postdoctoral (research associate) position is anticipated starting > immediately for a candidate interested in studying the molecular > mechanisms of stress responses and other environmental adaptations in > Aspergillus nidulans using functional genomics and other modern > molecular genetic techniques. For additional details visit our homepage > at http://microbiology.okstate.edu/faculty/prade . Candidates will be > working in close contact, and are encouraged to strongly interact and > participate, with, the new and state-of-the-art bioinformatics program. > A Ph.D. with training in molecular biology and or cellular biology is > required. Experience in large-scale cloning, mutagenesis and PCR > approaches are desirable. > > Interested candidates should send letter of interest, curriculum vitae, > name, address and phone number of three references to: R.A. Prade, > Department of Microbiology and Molecular Genetics, Oklahoma State > University. 306 LSE, Stillwater, OK 74078-3020. Deadline for > applications is March 15 1999. E-mail ayoubi@mendel.okstate.edu . > Oklahoma State University is an Equal Opportunity/Affirmative Action > Employer. Women and Minorities are Encouraged to Apply. > > > == Graham Dellaire, Ph.D. Moderator bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Thu Jan 28 22:00:00 1999 Path: biosci!biosci!not-for-mail From: "Pieter van Santen" Newsgroups: bionet.molbio.recombination Subject: The Main Metabolic Pathways on Internet Date: 29 Jan 1999 10:13:38 -0800 Organization: World Access / Planet Internet Lines: 25 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <78stoi$88q@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleague, The Main Metabolic Pathways on Internet are available. Also the PC-version can be downloaded. I hope you like the site and program. The adres is: http://home.wxs.nl/~pvsanten/mmp/mmp.html Regards Pieter van Santen == Moderated bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com