From owner-recombination@net.bio.net Wed Mar 10 22:00:00 1999 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.recombination Subject: SciMedWeb news Date: 11 Mar 1999 07:42:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Approved: genestructure@yahoo.com Distribution: world Message-ID: <7c8o98$rvg@net.bio.net> NNTP-Posting-Host: net.bio.net SciMedWeb, molecular aspects of breast cancer: http://www.geocities.com/HotSprings/Spa/3430/intro1.htm Breast cancer metastasis - Papers Nov-Dec 1998: http://www.geocities.com/HotSprings/Spa/3430/1998_6.htm Estrogen receptors and breast cancer - Papers Nov-Dec 1998: http://www.geocities.com/HotSprings/Spa/3430/er1998_6.htm Breast cancer and gene therapy - Papers 1998: http://www.geocities.com/HotSprings/Spa/3430/bcther98.htm > > == Moderated bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Wed Mar 10 22:00:00 1999 Path: biosci!biosci!not-for-mail From: ouzounis@ebi.ac.uk (Christos Ouzounis) Newsgroups: bionet.molbio.recombination Subject: Discovery Notes @ Bioinformatics Date: 11 Mar 1999 08:00:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7c8pac$ls@net.bio.net> NNTP-Posting-Host: net.bio.net THE BIOINFORMATICS JOURNAL The editors of Bioinformatics have now introduced a new category of paper to the journal called "Discovery Notes". This is intended for the reporting of biologically interesting discoveries using computational techniques. Topics may include sequence motif detection, structural similarities, gene structure prediction, comparative genomics, metabolic pathways and other aspects of computational molecular biology. The new category complements the original papers, reviews and applications notes that are presently published. The description of the analysis can be up to 2 pages ong (1000 words) including one/two figures. An abstract is not required. Sequences must be freely available in the database and the results of the analyses should not have been published elsewhere. The paper will be peer-reviewed. For more information see: http://www.oup.co.uk/bioinformatics From the BIOINFORMATICS office at the EBI > > == Moderated bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Thu Mar 11 22:00:00 1999 Path: biosci!biosci!not-for-mail From: pradipg@aol.com (PradipG) Add to Address Book Newsgroups: bionet.molbio.recombination Subject: CANCER CELL/TISSUE RESPIRATION IN CULTURE Date: 12 Mar 1999 08:18:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7cbeo0$ced@net.bio.net> NNTP-Posting-Host: net.bio.net CANCER CELL/TISSUE RESPIRATION IN CULTURE We have used O2/CO2 Respirometer (Micro-Oxymax) to measure O2 consumption and CO2 production in normal and cancer cell/tissue. The results are very exciting. We could easily differentiate O2 consumption between normal and cancer cells and suppression of respiration by anticancer agents. Following cell lines and tissue were used for the experiment. CANCER CELL/TISSUE: 1. Rat prostate cancer cell line (MAT-LyLu) 2. Human prostate cancer cell line (PC-3) 3. Rat prostate cancer tissue from MAT-LyLu cells implanted rats. 4. Metastatic lung from MAT-LyLu cells implanted rats. 5. Prostate cancer cells exposed to anticancer agent, gossypol. NORMAL CELL/TISSUE: 1. Normal prostate tissue from rat. 2. Lung tissue from rats. 3. Adipocytes from normal rats. If you are interested about this technology and the observation, please e-mail your postal address. I will send you all information. P.K. Ghosh, Ph.D. == Moderated bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Mon Mar 15 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Todd Caldwell Newsgroups: bionet.molbio.recombination,bionet.molbio.methds-reagnts,bionet.announce,bionet.cellbiol,bionet.jobs,bionet.jobs.offered,bionet.jobs.wanted,bionet.journals.contents,bionet.journals.letters.biotechniques,bionet.journals.letters.tibs,bionet.journals.note,bionet.women-in-bio,bionet.users.addresses,sci.research.careers,sci.research,sci.med.laboratory Subject: ? Advanced Tissue Sciences ? - Request Date: 16 Mar 1999 09:13:48 -0800 Organization: Erinet Online Communications Lines: 8 Sender: nospam@net.bio.net Approved: news@net.bio.net Distribution: world Message-ID: <36ECAA2D.B4B48E18@erinet.com> NNTP-Posting-Host: net.bio.net X-Authentication-Warning: backdraft.philabs.research.philips.com: smap set sender to using -f X-Trace: news.erinet.com 921479401 22145 207.90.117.7 (15 Mar 1999 06:30:01 GMT) X-Complaints-To: usenet@news.erinet.com X-Mailer: Mozilla 4.05 [en] (Win95; I) Xref: biosci bionet.molbio.recombination:695 bionet.molbio.methds-reagnts:74814 bionet.announce:4987 bionet.cellbiol:11494 bionet.jobs.offered:12076 bionet.jobs.wanted:27835 bionet.journals.contents:3605 bionet.journals.letters.biotechniques:215 bionet.journals.letters.tibs:106 bionet.journals.note:1190 bionet.women-in-bio:8624 bionet.users.addresses:3750 Does anyone know anything about Advanced Tissue Sciences? I am looking at them as a prospective employer, and am interested in some educated insights into the company's reputation and potential. Thank you for any reply. Todd C. (caldtc@erinet.com) From owner-recombination@net.bio.net Tue Mar 16 22:00:00 1999 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.recombination Subject: Postdoctoral Fellowship in BIOINFORMATICS Date: 17 Mar 1999 00:32:33 -0800 Organization: The European Bioinformatics Institute Lines: 99 Approved: genestructure@yahoo.com Distribution: world Message-ID: <7cnpb1$ndc@net.bio.net> NNTP-Posting-Host: net.bio.net Laboratoire Européen de Biologie Moléculaire European Molecular Biology Laboratory Europäisches Laboratorium für Molekularbiologie March 1999 European Bioinformatics Institute - EMBL Outstation Postdoctoral Fellowship in BIOINFORMATICS Funded by the TMR Programme of the European Commission Commencing Date: As soon as possible We are seeking candidates interested in Bioinformatics, with a Ph.D. in any scientific field, preferably with strong computational skills. Our main objective is large-scale genome sequence analysis with focus on problems of biological interest. We are continuously analysing complete genome sequences as they are being produced, and one possible project is the mining of the results database. The TMR Network at the EBI is one of the largest and more active training activities of scientists at the post-doctoral level in the field of bioinformatics in Europe. The network consists of the following laboratories: - Research Programme, The European Bioinformatics Institute, Cambridge, UK - Department of Bioinformatics, Max-Delbr½ck-Center for Molecular Medicine (MDC), Berlin, Germany - Protein Design Group, National Center of Biotechnology, CSIC, Madrid, Spain - Biophysics Unit, Department of Biochemistry, University of Athens, Athens, Greece - Biotechnology Centre, University of Oslo, Oslo - Norway - Biocomputing Unit, Research Institute of Molecular Biology, Pomezia, Rome, Italy Individuals are eligible to apply for this post if they are nationals of an EU member state or associated state wishing to work away from their home country. Applicants should in general have a Ph.D. and be under 35 years old. The fellowships are not intended for postdocs already working in the chosen country. Contract: A contract of 18 months' duration will be offered to the successful candidate. Please send your CV, list of publications, a brief statement of research interests and the names of two referees, quoting reference no. 99/PD/TMR(3) to: The Head of Human Resources, EMBL, Postfach 10.2209, D-69012 Heidelberg, Germany Fax: +49 6221 387 555 email: jobs@EMBL-Heidelberg.de == Moderated bionet.genome.gene-structure _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Tue Mar 23 22:00:00 1999 Path: biosci!biosci!not-for-mail From: "Ted Aanstoos" Newsgroups: bionet.molbio.recombination Subject: The God Gene Date: 24 Mar 1999 06:58:09 -0800 Organization: The University of Texas at Austin, Lines: 29 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7daui1$os2@net.bio.net> NNTP-Posting-Host: net.bio.net Hello Group I read a few months or so ago that someone had found a gene seemingly linked to a proclivity for religious faith. Could anyone in this group cite an article for me on this topic? Many thanks, Ted ---------------- note from moderator: This is not necessarily on topic but I thought it was interesting all the same... G. Dellaire Moderator === Moderated bionet.genome.gene-structure _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Mon Mar 29 23:00:00 1999 Path: biosci!biosci!not-for-mail From: biohazard1999@my-dejanews.com Newsgroups: bionet.molbio.recombination Subject: Resources in molecular biology at Date: 30 Mar 1999 03:12:53 -0800 Organization: Deja News - The Leader in Internet Lines: 10 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7dqbjl$t0i@net.bio.net> NNTP-Posting-Host: net.bio.net Moderated bionet.genome.gene-structure _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Wed Mar 31 23:00:00 1999 Path: biosci!biosci!not-for-mail From: Abdellah Djebli Newsgroups: bionet.molbio.recombination Subject: A new WWW accessible Bioinformatics Date: 31 Mar 1999 21:47:14 -0800 Organization: Hospital for Sick Children Lines: 65 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7dv192$de9@net.bio.net> Reply-To: help@bioinfo.sickkids.on.ca NNTP-Posting-Host: net.bio.net The BSC (Bioinformatics Supercomputing Centre) has been established at the Hospital for Sick Children in Toronto, Ontario, Canada. The BSC has recently acquired Canada's most powerful supercomputer devoted to biological and bioinformatics research. In addition to offering the online resources listed below, the BSC provides assistance for bioinformatics programs, databases etc. Contact us (see below) for support. The BSC's Mission: To be the Canadian leader in bioinformatics through innovative computational research and provision of training, support, software, and database access. Currently, the BSC provides, free of charge, the following internet accessible resources to scientists: 1) High-speed BLAST of the most current databases on the Origin 2000 supercomputer (SGI) 2) Canadian Node of the human Genome Data Base (GDB) 3) The Cystic Fibrosis Mutation Database 4) Software enhancement/creation 5) Bioinformatics Training/Data analysis 6) The GCG package and its new web interface--SeqWeb, available to researchers within the Hospital for Sick Children Visit our website http://www.bioinfo.sickkids.on.ca Call our Help Desk at (416) 813-8877 E-mail help@bioinfo.sickkids.on.ca Brenda Muskat, Analyst/Trainer Bioinformatics Supercomputing Centre Hospital for Sick Children 555 University Ave. Elm 10-104 Toronto, Ontario M5G 1X8 === Moderated bionet.genome.gene-structure _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Wed Mar 31 23:00:00 1999 Path: biosci!biosci!not-for-mail From: Abdellah Djebli Newsgroups: bionet.molbio.recombination Subject: A new WWW accessible Bioinformatics Date: 31 Mar 1999 21:42:18 -0800 Organization: Hospital for Sick Children Lines: 65 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7dv0vq$922@net.bio.net> Reply-To: help@bioinfo.sickkids.on.ca NNTP-Posting-Host: net.bio.net The BSC (Bioinformatics Supercomputing Centre) has been established at the Hospital for Sick Children in Toronto, Ontario, Canada. The BSC has recently acquired Canada's most powerful supercomputer devoted to biological and bioinformatics research. In addition to offering the online resources listed below, the BSC provides assistance for bioinformatics programs, databases etc. Contact us (see below) for support. The BSC's Mission: To be the Canadian leader in bioinformatics through innovative computational research and provision of training, support, software, and database access. Currently, the BSC provides, free of charge, the following internet accessible resources to scientists: 1) High-speed BLAST of the most current databases on the Origin 2000 supercomputer (SGI) 2) Canadian Node of the human Genome Data Base (GDB) 3) The Cystic Fibrosis Mutation Database 4) Software enhancement/creation 5) Bioinformatics Training/Data analysis 6) The GCG package and its new web interface--SeqWeb, available to researchers within the Hospital for Sick Children Visit our website http://www.bioinfo.sickkids.on.ca Call our Help Desk at (416) 813-8877 E-mail help@bioinfo.sickkids.on.ca Brenda Muskat, Analyst/Trainer Bioinformatics Supercomputing Centre Hospital for Sick Children 555 University Ave. Elm 10-104 Toronto, Ontario M5G 1X8 === Moderated bionet.genome.gene-structure _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-recombination@net.bio.net Wed Mar 31 23:00:00 1999 Path: biosci!biosci!not-for-mail From: "Graham Dellaire, Ph.D." Newsgroups: bionet.molbio.recombination Subject: Problems expressing 50-60 kDa protein in Date: 1 Apr 1999 03:52:27 -0800 Organization: MRC Human Genetics Unit Lines: 64 Sender: daemon@net.bio.net Approved: genestructure@yahoo.com Distribution: world Message-ID: <7dvmlr$fgt@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all, I am trying to express a 1.65 kb cDNA in E. coli using the pET32+ system with BL21(DE3) bugs. The protein including the trxa/his tag should be about 50 to 60 kDa. The protocol I used is as follows: first I grow up a 5 ml culture overnight from a single colony; then in the morning to inoculate 10 mls of medium with 100 ul of the o/n culture; two hours later I start induction with 1 mM IPTG and collect samples at 1 h, 2 hr, 4 hrs post-induction and o/n. By coomassie staining I see basically no induction at all. The protein sequence has quite a few arginines and lysines (so fairly basic) and perhaps this is a problem, although solubility may not be an issue! If anyone has any experience in expressing a large protein in E. coli I would like to hear how you got the thing to be expressed efficiently. I am currently trying 0.1 mM IPTG induction overnight at 37 and also I am trying the host strain ad494(DE3) and ad494(DE3)-lys. Any and all tips/protocols are much appreciated. Cheers, Graham Dellaire -- ******************** Graham Dellaire, Ph.D. MRC Human Genetics Unit Western General Hospital Crewe Road Edinburgh, UK EH4 2XU Fax: +44 (0)131 343 2620 Phone: +44 (0)131 332 2471 ******************** === Moderated bionet.genome.gene-structure _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com