From owner-recom@hgmp.mrc.ac.uk Sun Feb 6 12:59:06 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D942917AE5; Sun, 6 Feb 2000 12:59:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id C3E1217AE1; Sun, 6 Feb 2000 12:59:04 +0000 (GMT) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: leahjg@pacbell.net (Leah Jaffe-Greenwood) Subject: genomics web site Date: 2 Feb 2000 19:02:20 -0000 Organization: Pangea Systems Message-Id: <20000206125904.C3E1217AE1@mercury.hgmp.mrc.ac.uk> Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com From owner-recom@hgmp.mrc.ac.uk Sun Feb 13 16:29:33 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B261017A68; Sun, 13 Feb 2000 16:29:32 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id D62DC17A5A; Sun, 13 Feb 2000 16:29:30 +0000 (GMT) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: Marcus Wernitz Subject: Position for PhD student (BAT IIa/2-O) Date: Wed, 09 Feb 2000 11:58:44 +0100 Organization: University Halle, Germany Message-Id: <20000213162930.D62DC17A5A@mercury.hgmp.mrc.ac.uk> Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk Position for PhD student (BAT IIa/2-O) available at Martin-Luther-University Halle-Wittenberg, Faculty of Agriculture,Phytopathology and Plant Protection Mechanisms of acquired resistance: Isolation and characterization of salicylate-responsive genes of broad bean (Vicia faba) Chemical induction of resistance has been discussed as a novel plant protection strategy, and mechanisms underlying acquisition of resistance have been investigated intensively (Sticher et al., 1997). In broad bean, exogenously applied salicylate induces local, but not systemic resistance against the broad bean rust fungus (Uromyces fabae). This resistance has been attributed to the induction of an apoplastic antifungal basic PR-1 protein, and, interestingly, only infection structures invading the leaf are sensitive to the protein. The inhibitor thus acts infection structure-specifically (Rauscher et al., 1999). In order to understand the complexity of the mechanism of acquired resistance we have be-gun to clone salicylate-responsive broad bean genes, using differential subtractive hybridi-zation (Ace et al., 1994). Several cDNAs exhibiting differential hybridization patters have been obtained, and for some of these salicylate-inducibility has been confirmed by RT-PCR. The aim of this project is to identify and characterize salicylat-responsive genes of Vicia faba that may be involved in defense against fungal plant pathogens. During the course of the project, general methods of molecular genetics, e.g. RT-PCR, differential hybridiza-tion, DNA sequencing and heterologous expression of the isolated cDNAs will be applied. Financial support will be given for two years, with a possible 1-year extension, depending on the success of the project. The position is available immediately. Requirements: Masters or Diploma (Germany) in Phytopathology, Microbiology, Botany, Biochemistry or related fields, and experience with plant pathogenic fungi and techniques used in molecular biology. For application or further information, please contact: Prof. Dr. Holger B. Deising Martin-Luther-University Halle-Wittenberg Faculty of Agriculture Phytopathology and Plant Protection Ludwig-Wucherer-Str. 2 D-06099 Halle (Saale) e-mail: deising@landw.uni-halle.de phone: +345-5522660 Literature Ace, C. I., M. Balsamo, L. T. Le und W. C. Okulicz (1994): Isolation of progesterone-dependent complementary desoxyribonucleic acid fragments from rhesus monkey endometrium by sequential subtractive hybridization and polymerase chain reaction amplification. Endocrinology 134, 1305-1309. Rauscher, M., A. L. Ádám, S. Wirtz, R. Guggenheim, K. Mendgen und H. B. Deising (1999): PR-1 inhibits the differentiation of rust infection hyphae in leaves of acquired resistant broad bean. The Plant Journal 19, 625-635. Sticher, L., B. Mauch-Mani und J.-P. Métraux (1997): Systemic acquired resistance. Annual Review of Phytopathology 35, 235-270. ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com