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From: scott@codon.nih.gov (Scott Young)
Subject: In vitro testing of LoxP or FRT constructs
Date: 30 Mar 2000 20:03:23 +0100
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Can anyone supply protocols for in vitro testing of
constructs with pairs of
LoxP or FRT sites, including commercial sources of
reagents?

Thank you very much.

********************************************
 W. Scott Young, III, M.D., Ph.D.
 NIMH TEL: 301-496-8767   FAX: 301-402-6473
 http://intramural.nimh.nih.gov/lcmr/snge/
 E Mail : scott@codon.nih.gov
********************************************

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From owner-recom@hgmp.mrc.ac.uk  Wed Apr  5 23:19:54 2000
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From: garfinke@ncifcrf.gov ("David J. Garfinkel")
Subject: Postdoctoral position to study Ty
Date: 5 Apr 2000 14:50:58 +0100
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 The position is part of the Division of Basic
 Sciences of the 
 National Cancer Institute and is located at the
 Frederick Cancer 
 Research and Development Center in Frederick, MD. 
 The National 
 Institutes of Health is an equal opportunity
 employer and encourages 
 all qualified women and minorities to apply. 
 Selection for the 
 position will be based solely on merit, with no
 discrimination for 
 non-merit reasons such as race, marital status,
 physical or mental 
 disability, sexual orientation or membership or
 nonmembership in an 
 employee organization.  Salary range for this
 position is 
 $31,500-38,020.
 
 Interested candidates should submit a CV and names
 of three references to:
 
 David J. Garfinkel, PhD
 Gene Regulation and Chromosome Biology Laboratory
 Building 539, Room 152J
 NCI-Frederick Cancer Research and Development Center
 PO Box B
 Frederick, MD 21702-1201
 
 Alternatively, your CV and names of references can
 be faxed to 
 301-846-6911 or e-mailed to
 garfinke@mail.ncifcrf.gov
 
 
 
 David J. Garfinkel
 National Cancer Institute/NIH 539-152J
 Frederick Cancer Research and Development Center
 PO Box B
 Frederick, MD 21702-1201
 
 voice: 301-846-5604
 fax:   301-846-6911
 e-mail:  garfinke@ncifcrf.gov
 
 Shipping (Fed Ex shipments, etc)
 
 David J. Garfinkel
 National Cancer Institute/NIH 539-152J
 Frederick Cancer Research and Development Center
 C/O Receiving
 1050 Boyles St.
 Frederick, MD21702-1201
 
 Receiving phone: 301-846-1172
 ---
 


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From owner-recom@hgmp.mrc.ac.uk  Mon Apr 17 14:19:15 2000
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Newsgroups: bionet.molbio.recombination
From: G.Dellaire@hgu.mrc.ac.uk ("G.Dellaire")
Subject: The Recombination Resources: Hot Papers
Date: 17 Apr 2000 14:15:33 +0100
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HOT PAPERS for March and April 2000 in Recombination
from the Recombination Pages!
See:
http://www.hgu.mrc.ac.uk/Users/Graham.Dellaire/Recompages/papers/march-april2000.html
 
--
********************
Graham Dellaire, Ph.D.
 
MRC Human Genetics Unit
Western General Hospital
Crewe Road
Edinburgh, UK
EH4 2XU

Fax: +44 (0)131 343 2620
Phone: +44 (0)131 332 2471
********************
 
 
<HR>
<HTML>

<HEAD>
<TITLE>The Recombination Resources: Hot Papers March
and April 2000</TITLE>
<META name="description" content="Hot Papers in the
field of Recombination, hosted by the MRC Human
Genetics Unit in Edinburgh">
     <META name="keywords" content="articles, papers,
journals, review,Chromatin, DNA, recombination,
protein, proteins, genome, graham, dellaire, , MRC,
function, structure, transcription, replication,
epigenetics, mieosis, mieotic, mitosis, somatic,
phosphate, signal transduction, phosphorylation,  ATM,
p53, rad51, rad54, spo11, mei9,  rad50,
Immunoglobulin, synaptic, synapse, synapsis, gene
conversion, gene, 4-way junction, holiday junction,
methylation,  gene therapy, cloning, one-sided,
one-ended, sythesis dependent, double-strand break
repair, single strand annealing, drosophila, yeast, s.
cerevesiae, s. pombe, c. elegans, mouse, murine,
mammalian, cancer, genomic stability, instability,
mais, site-specific, switch recombination, rag-1,
rag-2, DNApk, parp, ku70, ku80">

<center><p>
<table width=600><tr><td><table
width=100%><tr><td><font size=+2 face=garamond>
<B>THE RECOMBINATION PAGES</B></font><br>
<SCRIPT>
<!-- 
document.write("<CENTER>");
document.write("( Last update on:
"+document.lastModified);
document.write(" )</CENTER>");
// -->

</SCRIPT></td> 
<td ALIGN="RIGHT"><center><FONT SIZE="+2"
face=garamond>><FONT
COLOR="#FF0000">Recombination<br>Resources</FONT>
</center></FONT></td></tr></table><br>
<hr>
<table width=100%><tr><td>HOT PAPERS IN
RECOMBINATION</td><td width=30%>&nbsp;</td><td>MARCH
and APRIL, 2000</td></tr></table><hr>

<p>

<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10744977&dopt=Abstract
">The human rad52 protein exists as a heptameric
ring</a>. <br> Stasiak AZ, Larquet E, Stasiak A,
Muller S, Engel A, Van Dyck E, West SC, Egelman EH 
<br>
 Curr Biol 2000 Mar 23;10(6):337-40
 <p>

<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10705376&dopt=Abstract">Midgestation
lethality in mice deficient for the RecA-related gene,
Rad51d/Rad51l3.</a><br> 
 Pittman DL, Schimenti JC 
<br>
Genesis 2000 Mar;26(3):167-73
<p>
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10754554&dopt=Abstract">Links
between replication, recombination and genome
instability in
                       eukaryotes. </a><br>
Flores-Rozas H, Kolodner RD <br>Trends Biochem Sci
2000 Apr;25(4):196-200
<p>



<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10736227&dopt=Abstract
">Two Holliday Junction Resolving Enzymes in
Sulfolobus solfataricus. </a>
<br>
Kvaratskhelia M, White MF <br>
 J Mol Biol 2000 Apr 7;297(4):923-932
<p>
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10760268&dopt=Abstract">The
Holliday junction in an inverted repeat DNA sequence:
Sequence
effects on the structure of four-way junctions.</a>
<br>
Eichman BF, Vargason JM, Mooers BH, Ho PS <br>

Proc Natl Acad Sci U S A 2000 Apr 11;97(8):3971-3976
<p>

<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10747037&dopt=Abstract
">Homologous and non-homologous recombination
differentially affect
DNA damage repair in mice. </a>
<br>Essers J, van Steeg H, de Wit J, Swagemakers SM,
Vermeij M, Hoeijmakers JH,
Kanaar R <br>EMBO J 2000 Apr 3;19(7):1703-1710

<p>
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10725370&dopt=Abstract
">RecA stimulates sister chromatid exchange and the
fidelity of
double-strand break repair, but not gene targeting, in
plants transformed
 by agrobacterium. </a><br>Reiss B, Schubert I,
Kopchen K, Wendeler E, Schell J, Puchta H <br>
Proc Natl Acad Sci U S A 2000 Mar 28;97(7):3358-63<p>


<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10740922&dopt=Abstract
">Distinguishing recombination and intragenic gene
conversion by linkage
disequilibrium patterns. </a><br>
Wiehe T, Mountain J, Parham P, Slatkin M<br>Genet Res
2000 Feb;75(1):61-73<p>



                                   
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10725659&dopt=Abstract">XRCC3
is required for efficient repair of chromosome breaks
by
homologous recombination.</a> 
<br>Brenneman MA, Weiss AE, Nickoloff JA, Chen DJ<br>
Mutat Res 2000 Mar 20;459(2):89-97<p>



<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10733533&dopt=Abstract">A
novel pairing process promoted by escherichia coli
RecA protein: inverse
DNA and RNA strand exchange.</a>
<br>
Zaitsev EN, Kowalczykowski SC <br>Genes Dev 2000 Mar
15;14(6):740-9<p>



<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10747055&dopt=Abstract">RAD51
Is Required for Propagation of the Germinal Nucleus in
Tetrahymena thermophila.</a><br> 

Marsh TC, Cole ES, Stuart KR, Campbell C, Romero DP
<br>Genetics 2000 Apr;154(4):1587-1596<p>


 <a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10753777&dopt=Abstract
">Replication and recombination intersect.</a><br> 
Marians KJ <br>
 Curr Opin Genet Dev 2000 Apr;10(2):151-156
<p>
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10754550&dopt=Abstract">A
common structural core in proteins active in DNA
recombination and
replication. </a>
Egelman E <br>Trends Biochem Sci 2000
Apr;25(4):179-182<p>



                                                   
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10756007&dopt=Abstract
">Roles of adeno-associated virus rep protein and
human chromosome 19 in
site-specific recombination</a><br>
Young SM Jr, McCarty DM, Degtyareva N, Samulski RJ
<br>J Virol 2000 May;74(9):3953-66
<p>

<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10757755&dopt=Abstract">DNA
damage-inducible and RAD52-independent repair of DNA
double-strand breaks in saccharomyces
cerevisiae.</a><br> 
Moore CW, McKoy J, Dardalhon M, Davermann D, Martinez
M, Averbeck D <br>Genetics 2000 Mar;154(3):1085-99<p>




<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10757769&dopt=Abstract
">Use of a small palindrome genetic marker to
investigate mechanisms of
double-strand-break repair in mammalian cells.</a><br>

Li J, Baker MD <br>Genetics 2000 Mar;154(3):1281-9<p>



<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10757756&dopt=Abstract">Bipartite
structure of the SGS1 DNA helicase in saccharomyces
cerevisiae.</a><br>
Mullen JR, Kaliraman V, Brill SJ <br>Genetics 2000
Mar;154(3):1101-14<p>




<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10757799&dopt=Abstract">Mouse
RAD54 Affects DNA Double-Strand Break Repair and
Sister
Chromatid Exchange. </a><br>
Dronkert ML, Beverloo HB, Johnson RD, Hoeijmakers JH,
Jasin M, Kanaar R<br>Mol Cell Biol 2000 May
1;20(9):3147-3156<p>


<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10758163&dopt=Abstract">Adeno-associated
virus site-specifically integrates into a
muscle-specific
DNA region.</a><br> 
Dutheil N, Shi F, Dupressoir T, Linden RM <br>
Proc Natl Acad Sci U S A 2000 Apr 11;<p>

                                                      
 
<a
href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10758501&dopt=Abstract
">AtRAD1, a plant homologue of human and yeast
nucleotide excision repair
endonucleases, is involved in dark repair of UV
damages and
recombination.</a><BR>
Gallego F, Fleck O, Li A, Wyrzykowska J, Tinland B<br>
Plant J 2000 Mar;21(6):507-518<p>


 <hr>

<table border=2 width=100%> <tr><td><CENTER><a
href="../people.html">People in the
field</a></CENTER></td><td><CENTER><a
href="../conferences.html">Conferences</a></CENTER></td><td><CENTER><FONT
COLOR="#FF0000">Recombination
<br>Resources</font></CENTER></td><td><CENTER><a
href="http://www.bio.net:80/hypermail/RECOMBINATION/">RECOM
@
<br>Bionet</a></CENTER></td>
</tr></table>
<p>
<hr></td></tr></table>
</center><table><tr><td>
<img src="../../pict/chromo4.gif" width=30
height=30></td><td valign=center><a
href="mailto:Graham.Dellaire@hgu.mrc.ac.uk?SUBJECT=A
suggested Paper for the RECOMBINATION PAGES">Suggest a
HOT Paper </a></td></tr></table><p>



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From owner-recom@hgmp.mrc.ac.uk  Sun Apr 30 11:45:04 2000
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To: recom@net.bio.net
Newsgroups: bionet.molbio.recombination
From: chamankh@duke.usask.ca (MC)
Subject: Re: The Recombination Resources: Hot Papers
Date: 28 Apr 2000 04:07:35 +0100
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Dear Dr. Dellaire
 
Finally!! Glad to see something appearing in the
Recombination newsgroup after a long time. I loved
the page for the recent hot
papers and the people in the field page. I hope you
keep the good work updated.

Sincerely
Mahmood

 


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