From owner-recom@hgmp.mrc.ac.uk Sun May 21 12:51:42 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3854C17AC7; Sun, 21 May 2000 12:51:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id CDEE617A84; Sun, 21 May 2000 12:51:40 +0100 (BST) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: Todd Beaman Subject: Gateway cloning system Date: Thu, 18 May 2000 15:13:24 -0400 Message-Id: <20000521115140.CDEE617A84@mercury.hgmp.mrc.ac.uk> Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk Hello, I was wondering if anyone had experience with the GATEWAY cloning system from Gibco. The system works by lambda integration and excision enzymes and sites. It is restriction-enzyme-free subcloning into different expression vectors from an entry clone. Does the attB sites alter expression or cause problems when attached to your primers during PCR? Has anyone generated any destination vectors containing promoters not offered by Gibco? Thanks in advance, Todd ============================================================ > Todd W. Beaman > Phone: 718-430-2785 > e-mail: beaman@aecom.yu.edu > ============================================================== > > ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Send instant messages & get email alerts with Yahoo! Messenger. http://im.yahoo.com/ From owner-recom@hgmp.mrc.ac.uk Mon May 22 08:54:18 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id EE97C17AD9; Mon, 22 May 2000 08:54:17 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id CEFAE17ACA; Mon, 22 May 2000 08:54:15 +0100 (BST) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: mark.cauchi@med.monash.edu.au (Mark Cauchi) Subject: Recombinant DNA Techniques Course Date: 22 May 2000 08:24:04 +0100 Message-Id: <20000522075415.CEFAE17ACA@mercury.hgmp.mrc.ac.uk> Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk http://www.med.monash.edu.au/micro/department/dnacorse.m --- > ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Send instant messages & get email alerts with Yahoo! Messenger. http://im.yahoo.com/ From owner-recom@hgmp.mrc.ac.uk Sat May 27 13:04:10 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1A9B017AD6; Sat, 27 May 2000 13:04:10 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 12BED17AED; Sat, 27 May 2000 13:04:08 +0100 (BST) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: jzryd@vtx.ch (Jean-Pierre Zrÿd) Subject: Homolgous Recombination - pre-programme.rtf Date: 24 May 2000 13:20:36 +0100 Message-Id: <20000527120408.12BED17AED@mercury.hgmp.mrc.ac.uk> Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk Homologous Rec Symposium register up to June 5th 2000 check http://www.3eme-cycle.ch/pages/bioveg.html JP Zryd --- ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Kick off your party with Yahoo! Invites. http://invites.yahoo.com/