From owner-recom@hgmp.mrc.ac.uk Sun Aug 4 17:27:43 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id E748D17A65 for ; Sun, 4 Aug 2002 17:27:41 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B2EE717A7E for ; Sun, 4 Aug 2002 17:27:40 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 2225817A65; Sun, 4 Aug 2002 17:27:39 +0100 (BST) To: recom@net.bio.net Newsgroups: bionet.molbio.recombination From: Jude_Przyborski@med.uni-heidelberg.de Subject: Bacterial promoters Date: 24 Jul 2002 17:31:11 +0100 Message-Id: <20020804162739.2225817A65@mercury.hgmp.mrc.ac.uk> X-Razor-id: 2924ddad59d6b605d1fadb648bb14c8d51d94f80 Sender: owner-recom@hgmp.mrc.ac.uk Precedence: bulk Dear List, Whilst I have a bit of experience with parasite promoters, I admit to being a bit of an idiot as far as bacterial promoters are concerned. My problem: I would like to put a selectable marker under control of 2 promoters, one of parasite origin, and one bacterial. My parasite promoter is not well characterised, and therefore 1kb long. All bacterial promoters that I have looked at seem however to have a few ATGs scattered within them, which I think will cause a problem!! Is it feasible for me to locate the bacterial promoter upstream to the parasite one, adding however the shine-dalgano sequence just before the intended ATG? Can a promoter work over such a great distance? If not, then is it possible for me to place a really minimal promoter (-35, -10, SD ) of my own design downstream of the parasitic one , making sure that no ATGs get in the way. I only need enough expression to enable cloning in e.coli. Thanks in advance Jude Przyborski Parasitology University of Heidelberg Germany judep@web.de --- ===== Moderated bionet.genome.gene-structure __________________________________________________ Do You Yahoo!? Everything you'll ever need on one web page from News and Sport to Email and Music Charts http://uk.my.yahoo.com