From BIOSCI-REQUEST Mon Sep 19 07:20:54 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id HAA03518 for rna-list; Mon, 19 Sep 1994 07:20:54 -0700 Received: from oxmail.ox.ac.uk (oxmail.ox.ac.uk [129.67.1.1]) by net.bio.net (8.6.9/8.6.6) with SMTP id HAA03512 for ; Mon, 19 Sep 1994 07:20:48 -0700 Received: from bioch.ox.ac.uk by oxmail.ox.ac.uk with SMTP (PP) id <22364-0@oxmail.ox.ac.uk>; Mon, 19 Sep 1994 15:20:33 +0100 Received: from bio.bioch.ox.ac.uk by bioch.ox.ac.uk (5.0/SMI-SVR4) id AA02487; Mon, 19 Sep 1994 15:20:28 +0000 Received: by bio.bioch.ox.ac.uk (5.0/SMI-SVR4) id AA02757; Mon, 19 Sep 1994 15:20:26 +0000 Date: Mon, 19 Sep 1994 15:20:26 +0000 From: kalim@bioch.ox.ac.uk (Kalim Mir) Message-Id: <9409191420.AA02757@bio.bioch.ox.ac.uk> To: RNA@net.bio.net X-Sun-Charset: US-ASCII Content-Length: 85 Subscribe RNA News group. Kalim Mir Department of Biochemistry Oxford University UK From BIOSCI-REQUEST Mon Sep 26 07:49:16 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id HAA12187 for rna-list; Mon, 26 Sep 1994 07:49:16 -0700 Received: from sae.ssu.umd.edu (sae.ssu.umd.edu [131.118.32.1]) by net.bio.net (8.6.9/8.6.6) with SMTP id HAA12182 for ; Mon, 26 Sep 1994 07:49:13 -0700 From: pmp3321@sae.ssu.umd.edu Received: by sae.ssu.umd.edu (MX V3.3 VAX) id 1985; Mon, 26 Sep 1994 10:52:04 EDT Date: Mon, 26 Sep 1994 10:52:03 EDT To: rna@net.bio.net Message-ID: <009850B5.1B30B7E0.1985@sae.ssu.umd.edu> subscribe RNA Peter Palenchar From BIOSCI-REQUEST Thu Sep 29 12:41:38 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id MAA09626 for rna-list; Thu, 29 Sep 1994 12:41:38 -0700 Received: from sae.ssu.umd.edu (sae.ssu.umd.edu [131.118.32.1]) by net.bio.net (8.6.9/8.6.6) with SMTP id MAA09621 for ; Thu, 29 Sep 1994 12:41:34 -0700 From: pmp3321@sae.ssu.umd.edu Received: by sae.ssu.umd.edu (MX V3.3 VAX) id 11969; Thu, 29 Sep 1994 15:44:31 EDT Date: Thu, 29 Sep 1994 15:44:30 EDT To: rna@net.bio.net Message-ID: <00985339.75326D20.11969@sae.ssu.umd.edu> Subject: unusual bases I know this isn't really one of the topics of this list, but I was wondering if anyone out there knows where I can find some information on RNA containing unusual or modified purines and pyrimidines. Peter Palenchar From BIOSCI-REQUEST Thu Sep 29 17:10:08 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id RAA26387 for rna-list; Thu, 29 Sep 1994 17:10:08 -0700 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.9/8.6.6) with SMTP id RAA26383 for ; Thu, 29 Sep 1994 17:10:05 -0700 Received: by proton.chem.yale.edu (940715.SGI.52/930416.SGI.AUTO) for rna@net.bio.net id AA17871; Thu, 29 Sep 94 20:10:04 -0400 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <9409300010.AA17871@proton.chem.yale.edu> Subject: Re: unusual bases To: rna@net.bio.net Date: Thu, 29 Sep 1994 20:10:03 -0400 (EDT) In-Reply-To: <00985339.75326D20.11969@sae.ssu.umd.edu> from "pmp3321@sae.ssu.umd.edu" at Sep 29, 94 03:44:30 pm Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL23] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 651 Peter, > I know this isn't really one of the topics of this list, Well, sure it is. > but I was wondering if anyone out there knows where I can find some > information on RNA containing unusual or modified purines and pyrimidines. I don't have it with me so can't give a precise citation, but one of the last few NARs has a compilation of modified bases with references to the original literature. Cheers, -- | Dave Schweisguth Internet: dcs@proton.chem.yale.edu MIME spoken here | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 | | For complying with the NJ Right To Know Act: Contents partially unknown. | From BIOSCI-REQUEST Thu Sep 29 18:25:08 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id SAA00782 for rna-list; Thu, 29 Sep 1994 18:25:08 -0700 Received: from PORTIA.CALTECH.EDU (portia.caltech.edu [131.215.51.154]) by net.bio.net (8.6.9/8.6.6) with ESMTP id SAA00779 for ; Thu, 29 Sep 1994 18:25:05 -0700 From: EARN@portia.caltech.edu Received: from PORTIA.CALTECH.EDU by PORTIA.CALTECH.EDU (PMDF V4.2-13 #4943) id <01HHOZMX76AOBL2MQ8@PORTIA.CALTECH.EDU>; Thu, 29 Sep 1994 18:24:47 PDT Date: Thu, 29 Sep 1994 18:24:47 -0700 (PDT) Subject: Is RNeasy? To: rna@net.bio.net Message-id: <01HHOZMX88VMBL2MQ8@PORTIA.CALTECH.EDU> X-Envelope-to: rna@net.bio.net X-VMS-To: IN%"rna@net.bio.net" MIME-version: 1.0 Content-transfer-encoding: 7BIT Now that the silence has been broken, I'd like to ask: Has anyone tried Qiagen's new RNeasy kit to prepare bacterial RNA for RNase protection analysis? I wonder if those delicate bacterial messages survive well with the cell pelleting and 5 minute lysozyme treatment steps. It certainly looks like a timesaving alternative to the standard hot phenol/whole culture procedures with their endless rounds of organic extraction and alcohol precipition, but naturally I'm skeptical. So, has anyone tried this? Any thoughts or predictions from those who haven't? Thanks in advance, Eric Arn Abelson Laboratory Caltech ------------------------------------------------------------------------------ one sorry lobe of the primordial undermind "Oh, the snot has caked against my pants, it has turned into crystal. There's a bluebird sitting on a branch, I guess I'll take my pistol. I've got it in my hand...." EARN@portia.caltech.edu - A. Lee From BIOSCI-REQUEST Thu Sep 29 19:01:32 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id TAA03011 for rna-list; Thu, 29 Sep 1994 19:01:32 -0700 Received: from cs.utah.edu (cs.utah.edu [128.110.4.21]) by net.bio.net (8.6.9/8.6.6) with SMTP id TAA02987 for ; Thu, 29 Sep 1994 19:01:29 -0700 Received: from adenosine.pharm.utah.edu by cs.utah.edu (5.65/utah-2.21-cs) id AA10651; Thu, 29 Sep 94 20:01:22 -0600 Date: Thu, 29 Sep 94 20:05:20 -0600 From: davis@adenosine.pharm.utah.edu (Darrell R. Davis) Posted-Date: Thu, 29 Sep 94 20:05:20 -0600 Message-Id: <9409300205.AA23997@adenosine.pharm.utah.edu> Received: by adenosine.pharm.utah.edu (911016.SGI/5.51) id AA23997; Thu, 29 Sep 94 20:05:20 -0600 To: dcs@proton.chem.yale.edu Cc: rna@net.bio.net In-Reply-To: Dave Schweisguth's message of Thu, 29 Sep 1994 20:10:03 -0400 (EDT) <9409300010.AA17871@proton.chem.yale.edu> Subject: unusual bases on Thu, 29 Sep 1994 20:10:03 -0400 (EDT), dcs@proton.chem.yale.edu (Dave Schweisguth) said: > I know this isn't really one of the topics of this list, >> Well, sure it is. > but I was wondering if anyone out there knows where I can find some > information on RNA containing unusual or modified purines and pyrimidines. >> I don't have it with me so can't give a precise citation, but one of the >> last few NARs has a compilation of modified bases with references to the >> original literature. That is probably a good place to start. For the most part it is just a compilation as was stated. Limbach, Crain, and McCloskey (1994) NAR 22, 2183-2196. -------------------------------------------------------------- Darrell R. Davis Medicinal Chemistry University of Utah -------------------------------------------------------------- From BIOSCI-REQUEST Fri Sep 30 05:43:22 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id FAA29656 for rna-list; Fri, 30 Sep 1994 05:43:22 -0700 Received: from selway.umt.edu (selway.umt.edu [150.131.14.2]) by net.bio.net (8.6.9/8.6.6) with SMTP id FAA29651 for ; Fri, 30 Sep 1994 05:43:21 -0700 Received: by selway.umt.edu (5.65/DEC-Ultrix/4.3) id AA18697; Fri, 30 Sep 1994 06:43:22 -0600 Message-Id: <9409301243.AA18697@selway.umt.edu> Subject: Re: unusual bases To: rna@net.bio.net Date: Fri, 30 Sep 1994 06:43:21 -0600 (MDT) From: "Michael van Waes" In-Reply-To: <00985339.75326D20.11969@sae.ssu.umd.edu> from "pmp3321@sae.ssu.umd.edu" at Sep 29, 94 03:44:30 pm X-Mailer: ELM [version 2.4 PL23] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7BIT Content-Length: 349 > > I know this isn't really one of the topics of this list, but >I was wondering if anyone out there knows where I can find some information >on RNA containing unusual or modified purines and pyrimidines. > > Peter Palenchar > Could you specify what information? RNAs involved in translation (rRNA, tRNA) are heavily modified. Cheers MvW From BIOSCI-REQUEST Fri Sep 30 07:52:50 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id HAA06902 for rna-list; Fri, 30 Sep 1994 07:52:50 -0700 Received: from sae.ssu.umd.edu (sae.ssu.umd.edu [131.118.32.1]) by net.bio.net (8.6.9/8.6.6) with SMTP id HAA06899 for ; Fri, 30 Sep 1994 07:52:47 -0700 From: pmp3321@sae.ssu.umd.edu Received: by sae.ssu.umd.edu (MX V3.3 VAX) id 14545; Fri, 30 Sep 1994 10:51:41 EDT Date: Fri, 30 Sep 1994 10:51:39 EDT To: rna@net.bio.net Message-ID: <009853D9.B6899BE0.14545@sae.ssu.umd.edu> Subject: unusual bases It seems that I wasn't clear enough with my original request. I was thinking about bases in ribozymes, self-splicing intron or viruses to be specific. Thank-you for the information I have already recieved. Peter From BIOSCI-REQUEST Fri Sep 30 08:21:13 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id IAA08725 for rna-list; Fri, 30 Sep 1994 08:21:13 -0700 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.9/8.6.6) with SMTP id IAA08706 for ; Fri, 30 Sep 1994 08:21:08 -0700 Received: by proton.chem.yale.edu (940715.SGI.52/930416.SGI.AUTO) for rna@net.bio.net id AA27384; Fri, 30 Sep 94 11:21:09 -0400 From: rife@proton.chem.yale.edu (Jason Rife) Message-Id: <9409301521.AA27384@proton.chem.yale.edu> Subject: Re:unusual bases To: rna@net.bio.net Date: Fri, 30 Sep 1994 11:21:09 -0400 (EDT) X-Mailer: ELM [version 2.4 PL23] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 410 Dear Peter- The only recent collective reference that I know an article in NAR a couple of monthes ago. NAR (1994) vol. 22 2183-2196. The authors report a summary and structures of all the known modified RNA nucleosides. However, the article is somewhat limited in its scope. Only the first characterization and synthesis references are given. But it is a good place to start. Good luck, Jason.