From BIOSCI-REQUEST Tue Nov 1 02:46:49 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id CAA21857 for rna-list; Tue, 1 Nov 1994 02:46:49 -0800 Received: from utsun.s.u-tokyo.ac.jp (utsun.s.u-tokyo.ac.jp [133.11.11.11]) by net.bio.net (8.6.9/8.6.6) with SMTP id CAA21844 for ; Tue, 1 Nov 1994 02:46:45 -0800 Received: from putaro.biochem.s.u-tokyo.ac.jp by utsun.s.u-tokyo.ac.jp (5.65/TISN-1.3MD/1.4) id AA12301; Tue, 1 Nov 94 19:46:47 +0900 Received: by putaro.biochem.s.u-tokyo.ac.jp (920330.SGI/920502.SGI.AUTO) for @utsun.s.u-tokyo.ac.jp:rna@net.bio.net id AA10075; Tue, 1 Nov 94 19:44:49 +0900 Date: Tue, 1 Nov 94 19:44:49 +0900 From: kiga@putaro.biochem.s.u-tokyo.ac.jp (Daisuke Kga) Message-Id: <9411011044.AA10075@putaro.biochem.s.u-tokyo.ac.jp> Subject: test Apparently-To: rna@net.bio.net Sorry, it's only a test. From BIOSCI-REQUEST Tue Nov 1 09:52:45 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id JAA14068 for rna-list; Tue, 1 Nov 1994 09:52:45 -0800 Received: from alamut.lifesci.ucla.edu (Alamut.LIFESCI.UCLA.EDU [128.97.8.84]) by net.bio.net (8.6.9/8.6.6) with SMTP id JAA14052 for ; Tue, 1 Nov 1994 09:52:36 -0800 Received: from smtpgate.lifesci.ucla.edu by alamut.lifesci.ucla.edu (Sendmail 4.1/1.00-lifesci) id AA10890; Tue, 1 Nov 94 09:49:38 PST Received: by smtpgate.lifesci.ucla.edu with Microsoft Mail id <2EB68080@smtpgate.lifesci.ucla.edu>; Tue, 01 Nov 94 09:53:04 PST From: "Zeiler, Brian M'bio" To: RNA Subject: RNAse ZAP Date: Tue, 01 Nov 94 09:51:00 PST Message-Id: <2EB68080@smtpgate.lifesci.ucla.edu> Encoding: 12 TEXT X-Mailer: Microsoft Mail V3.0 I just bought some RNaseZAP from Ambion and it seems to be working as advertised so far. Has anyone had any experience with this product? Does it really work? Does it interfere with any reactions after treatment? Does anyone use it on tips? Thanks in advance for any insight. Brian Zeiler brianz@microbio.lifesci.ucla.edu I am not associated with Ambion From BIOSCI-REQUEST Thu Nov 3 11:24:45 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id LAA00253 for rna-list; Thu, 3 Nov 1994 11:24:45 -0800 Received: from model.phr.utexas.edu (model.phr.utexas.edu [128.83.164.100]) by net.bio.net (8.6.9/8.6.6) with SMTP id LAA00234; Thu, 3 Nov 1994 11:24:22 -0800 Received: by model.phr.utexas.edu (931110.SGI/921111.SGI.AUTO) for rna@net.bio.net id AA08314; Thu, 3 Nov 94 13:24:27 -0600 From: "David G. Rhodes" Message-Id: <9411031324.ZM8312@model.phr.utexas.edu> Date: Thu, 3 Nov 1994 13:24:17 -0500 X-Mailer: Z-Mail (3.1.0 22feb94 MediaMail) To: bioforum@net.bio.net, biophysics@net.bio.net, human-genome-program@net.bio.net, rna@net.bio.net Subject: Export of mRNA Cc: rhodes@model.phr.utexas.edu Content-Type: text/plain; charset=us-ascii Mime-Version: 1.0 Maybe this is a naieve question, if so, I apologize to however many of you are there. I have asked several people whose area of expertise would suggest to me that they would know if anyone did, but... here we are... The question arises from a discussion of potential oligonucleotide targets in the nuclear "compartment". The author of one review suggests 3 possibilities: splicing (ok - there are ss's there), at transcription sites or at the binding of ribosomes (a transient opening), via triple helicies, or * export *. OK- here's the question. How does a cell know when to export a mature transcript to the cytoplasm? Is this process inhibited by ds structures? If so, does that mean that any mRNA's with significant secondary structure can't get out? Thanks for your input. - PLEASE reply to me directly, as I subscribe to only one of the groups to which this is posted. -- _____________________________________ O==O ________________________________ | David G. Rhodes | O==O | RHODES@MODEL.PHR.UTEXAS.EDU | | Pharmaceutics Division | O==O | | | College of Pharmacy | O==O | Phone: (512)471-4681 | | The University of Texas at Austin | O==O | Fax: (512)471-7474 | | Austin, TX 78712-1074 | O==O | }:) | |___________________________________| O==O |______________________________| O==O From BIOSCI-REQUEST Tue Nov 8 13:41:26 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id NAA11404 for rna-list; Tue, 8 Nov 1994 13:41:26 -0800 Received: from fisio.cinvestav.mx (fisio.cinvestav.mx [148.247.10.3]) by net.bio.net (8.6.9/8.6.6) with SMTP id NAA11393 for ; Tue, 8 Nov 1994 13:41:15 -0800 Received: from neuro5.fisio.cinvestav.mx by fisio.cinvestav.mx (4.1/SMI-4.1) id AA00575; Tue, 8 Nov 94 15:42:55 CST Received: by neuro5.fisio.cinvestav.mx (4.1/SMI-4.1) id AA00371; Tue, 8 Nov 94 15:48:33 CST Date: Tue, 8 Nov 94 15:48:33 CST From: jvega@neuro5.fisio.cinvestav.mx (Jesus Vega) Message-Id: <9411082148.AA00371@neuro5.fisio.cinvestav.mx> To: rna@net.bio.net Subject: subscribe SUBSCRIBE Jesus Vega Centro de Investigacion y de Estudios Avanzados Av. Instituto Politecnico Nacional 2508 col. San Pedro Zacatenco Mexico Tel. 754 02 00 ext. 5322 E-mail: jesus@lambda.gene.cinvestav.mx From BIOSCI-REQUEST Wed Nov 9 13:56:50 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id NAA06354 for rna-list; Wed, 9 Nov 1994 13:56:50 -0800 Received: from fisio.cinvestav.mx (fisio.cinvestav.mx [148.247.10.3]) by net.bio.net (8.6.9/8.6.6) with SMTP id NAA06338 for ; Wed, 9 Nov 1994 13:56:36 -0800 Received: from neuro5 (neuro5.fisio.cinvestav.mx) by fisio.cinvestav.mx (4.1/SMI-4.1) id AA01044; Wed, 9 Nov 94 14:52:20 CST Date: Wed, 9 Nov 1994 14:58:00 -0600 (CST) From: Jesus Vega X-Sender: jvega@neuro5 To: rna@net.bio.net Subject: Stability problems In-Reply-To: <9411082356.AB00713@fisio.cinvestav.mx> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I'ts a real pleassure to stay here! > I am making RNA transcripts ussing T7 for stability studies, but get very > inconsistent yields of messenger and I need very giant quantity of RNA for > structure solution experiments. Any incredible protocol out there? > > On the other hand, I need sintetize RNAs with RNA-fosforamidites for melting > studies but my transcript needs to be higer than 50 bases and the literature > seems to say that I can only have RNA less of 40 and say that the yield is very low. Do you have any suggestions? > Thanks!! From BIOSCI-REQUEST Thu Nov 10 14:09:49 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id OAA22934 for rna-list; Thu, 10 Nov 1994 14:09:49 -0800 Received: from fisio.cinvestav.mx (fisio.cinvestav.mx [148.247.10.3]) by net.bio.net (8.6.9/8.6.6) with SMTP id OAA22921 for ; Thu, 10 Nov 1994 14:09:41 -0800 Received: from neuro5 (neuro5.fisio.cinvestav.mx) by fisio.cinvestav.mx (4.1/SMI-4.1) id AA01673; Thu, 10 Nov 94 16:11:14 CST Date: Thu, 10 Nov 1994 16:16:51 -0600 (CST) From: Jesus Vega X-Sender: jvega@neuro5 To: rna@net.bio.net Subject: A lot of problems In-Reply-To: <9411090001.AA00449@neuro5.fisio.cinvestav.mx> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII > I'ts a real pleassure to stay here! > > I am making RNA transcripts ussing T7 for stability studies, but get very > inconsistent yields of messenger and I need very giant quantity of RNA for > structure solution experiments. Any incredible protocol out there? > > On the other hand, I need sintetize RNAs with RNA-fosforamidites for melting > studies but my transcript needs to be higer than 50 bases and the literature > seems to say that I can only have RNA less of 40 and say that the yield is very low. Do you have any suggestions? > > Thanks!! > From BIOSCI-REQUEST Fri Nov 11 10:07:56 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id KAA28966 for rna-list; Fri, 11 Nov 1994 10:07:56 -0800 Received: from itsa.ucsf.EDU (itsa.ucsf.EDU [128.218.1.14]) by net.bio.net (8.6.9/8.6.6) with SMTP id KAA28953 for ; Fri, 11 Nov 1994 10:07:49 -0800 Received: by itsa.ucsf.EDU (AIX 3.2/UCB 5.64/GSC4.22) id AA33599; Fri, 11 Nov 1994 10:06:01 -0800 Date: Fri, 11 Nov 1994 10:06:00 -0800 (PST) From: Huu Minh Tran To: rna@net.bio.net Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII subscribe rna end From BIOSCI-REQUEST Fri Nov 11 10:23:41 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id KAA00183 for rna-list; Fri, 11 Nov 1994 10:23:41 -0800 Received: from itsa.ucsf.EDU (itsa.ucsf.EDU [128.218.1.14]) by net.bio.net (8.6.9/8.6.6) with SMTP id KAA00180 for ; Fri, 11 Nov 1994 10:23:39 -0800 Received: by itsa.ucsf.EDU (AIX 3.2/UCB 5.64/GSC4.22) id AA13532; Fri, 11 Nov 1994 10:21:46 -0800 Date: Fri, 11 Nov 1994 10:21:45 -0800 (PST) From: Huu Minh Tran Subject: S&S Rad Free for Northern blot To: rna@net.bio.net Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Does any one have the experience(s) with the S&S Rad Free system? So far I have the problems with it. Any help will be very appreciated. The problem is that I have the background. I either have faint bands on ununiformed background or nothing at all. I sent the outline of the procedure which I had done to the manufacturer to ask them to help to trouble shoot; however, the replies that I got was that the way I was doing was right. Another thing I would like to ask about the immobilization of the RNAs on the membrane. I used the 0.2 micron S&S Max Strength. I UV xlinked with Fotodyne 310 illuminator (300nm) for 3 minutes and baked till dry for 15 min. at 80 celcius. Yet, after I stripped the blot and stained with Methylene Blue I got nothing on the blot. (I knew my RNAs were good and intact since I had hard copy of EtBr stained blot after transfering using 10X SSC). Thank you very much for any help or suggestion. Huu Tran From BIOSCI-REQUEST Thu Nov 17 00:46:36 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id AAA05176 for rna-list; Thu, 17 Nov 1994 00:46:36 -0800 Received: from ccvax.sinica.edu.tw (ccvax.sinica.edu.tw [140.109.4.15]) by net.bio.net (8.6.9/8.6.6) with ESMTP id AAA05166 for ; Thu, 17 Nov 1994 00:46:31 -0800 From: MBMTHSU@ccvax.sinica.edu.tw Received: from ccvax.sinica.edu.tw by ccvax.sinica.edu.tw (PMDF V4.3-10 #7209) id <01HJLCVMLMSG000MKJ@ccvax.sinica.edu.tw>; Thu, 17 Nov 1994 16:49:35 +0800 Date: Thu, 17 Nov 1994 16:49:35 +0800 Subject: subscribe To: rna@net.bio.net Message-id: <01HJLCVMMFPU000MKJ@ccvax.sinica.edu.tw> X-VMS-To: IN%"rna@net.bio.net" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT subscribe rna ming-tao hsu From BIOSCI-REQUEST Fri Nov 25 16:03:21 1994 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id QAA03145 for rna-list; Fri, 25 Nov 1994 16:03:21 -0800 Received: from dns1.uga.edu (dns1.uga.edu [128.192.1.9]) by net.bio.net (8.6.9/8.6.6) with SMTP id QAA03141 for ; Fri, 25 Nov 1994 16:03:20 -0800 Received: from merc.rx.uga.edu by dns1.uga.edu with SMTP id AA21677 (5.67b/IDA-1.5 for ); Fri, 25 Nov 1994 19:03:25 -0500 Received: from MERC/SpoolDir by merc.rx.uga.edu (Mercury 1.13); Fri, 25 Nov 94 19:03:24 EDT Received: from SpoolDir by MERC (Mercury 1.13); Fri, 25 Nov 94 19:03:09 EDT From: "CHANDU" Organization: UGA College of Pharmacy To: rna@net.bio.net Date: Fri, 25 Nov 1994 19:02:42 EST5EDT Subject: X-Confirm-Reading-To: "CHANDU" X-Pmrqc: 1 Priority: normal X-Mailer: Pegasus Mail v3.22 Message-Id: subscribe rna chandu *************************************************************** * CHANDRA SEKAR RAMANATHAN * * 253 E CLOVERHURST AVE., 17 * * ATHENS, GA 30605. * * PHONE(RES.) : (706) 613 2142 * * (OFF.) : (706) 542 5361 * ***************************************************************