From BIOSCI-REQUEST Fri Oct 6 15:35:34 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA19619 for rna-list; Fri, 6 Oct 1995 15:35:34 -0700 Received: from vidigal.nce.ufrj.br (vidigalgw.nce.ufrj.br [146.164.2.3]) by net.bio.net (8.6.12/8.6.6) with SMTP id PAA19558 for ; Fri, 6 Oct 1995 15:35:16 -0700 Received: from server.bioqmed.ufrj.br ([146.164.100.4]) by vidigal.nce.ufrj.br (4.1/SMI-4.1) id AA25864; Fri, 6 Oct 95 19:33:31 EST Received: from BIOQMED/MAILQUEUE by server.bioqmed.ufrj.br (Mercury 1.13); Fri, 6 Oct 95 19:45:33 GMT-3 Received: from MAILQUEUE by BIOQMED (Mercury 1.13); Fri, 6 Oct 95 19:45:22 GMT-3 From: "Orlando Bonifacio Martins" Organization: ICB/Dept. Bioquimica Medica/UFRJ To: rna@net.bio.net Date: Fri, 6 Oct 1995 19:45:21 GMT-3 Subject: tRNA(gln1) X-Confirm-Reading-To: "Orlando Bonifacio Martins" X-Pmrqc: 1 Priority: normal X-Mailer: Pegasus Mail for Windows (v2.01) Message-Id: <16BA3C41581@server.bioqmed.ufrj.br> I need some tRNA(Gln1) from E. coli to make experiments of charging. Does anyone know where could I buy it? Thanks in advance, Orlando Orlando Martins Tel: 55-21-270 0934 Dept Bioquimica Medica-ICB/CCS FAX: 55-21-270 8647 Univ Fed do Rio de Janeiro Email: omartins@server.bioqmed.ufrj.br Cx Postal 68041 RJ 21941-590 Brazil From BIOSCI-REQUEST Sun Oct 29 22:40:13 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id WAA17962 for rna-list; Sun, 29 Oct 1995 22:40:13 -0800 Received: from inet-tsb.toshiba.co.jp (inet-tsb.toshiba.co.jp [202.33.96.40]) by net.bio.net (8.6.12/8.6.6) with ESMTP id WAA17951 for ; Sun, 29 Oct 1995 22:40:06 -0800 From: kalim@arl.rdc.toshiba.co.jp Received: from tis2.tis.toshiba.co.jp by inet-tsb.toshiba.co.jp (8.6.12+2.5W/3.3W9-04/12/95) id PAA01451; Mon, 30 Oct 1995 15:38:56 +0900 Received: from tis10.tis.toshiba.co.jp (tis10) by tis2.tis.toshiba.co.jp (5.67+1.6W/6.4J.6-R05) id AA18643; Mon, 30 Oct 95 15:38:49 JST Received: from rdcgw.gwd.rdc.toshiba.co.jp (rdcgw) by tis10.tis.toshiba.co.jp (5.67+1.6W/6.4J.6-MHS-CNTML-R1) id AA22619; Mon, 30 Oct 95 15:38:46 JST Received: by mailhost.gwd.rdc.toshiba.co.jp (4.0/6.4J.6-EIS-V1.3) with SMTP id AA23314; Mon, 30 Oct 95 14:59:27 JST Received: by mailhost.arl.rdc.toshiba.co.jp (4.1/6.4J.6-EIS-V1.3) with SMTP id AA02962; Mon, 30 Oct 95 14:58:32 JST Date: Mon, 30 Oct 95 14:58:32 JST Message-Id: <9510300558.AA02962@arl.rdc.toshiba.co.jp> To: rna@net.bio.net Subject: crystal structures Hi Can anyone tell me, for which tRNA species the crystal structures been elucidated for (apart from tRNAphe from yeast). Do you have any refs? Know of any commercial (or other) supplies of these species? Also are there any RNA structures (eg group 1 introns) whose crystal structures have been elucidated but not published yet? Thanks in advance Kalim Mir From BIOSCI-REQUEST Mon Oct 30 05:45:43 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id FAA04086 for rna-list; Mon, 30 Oct 1995 05:45:43 -0800 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.12/8.6.6) with ESMTP id FAA04071 for ; Mon, 30 Oct 1995 05:45:37 -0800 Received: by proton.chem.yale.edu (950511.SGI.8.6.12.PATCH526/940406.SGI.AUTO) id IAA03131; Mon, 30 Oct 1995 08:44:20 -0500 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <199510301344.IAA03131@proton.chem.yale.edu> Subject: Re: crystal structures To: kalim@arl.rdc.toshiba.co.jp Date: Mon, 30 Oct 1995 08:44:20 -0500 (EST) Cc: rna@net.bio.net In-Reply-To: <9510300558.AA02962@arl.rdc.toshiba.co.jp> from "kalim@arl.rdc.toshiba.co.jp" at Oct 30, 95 02:58:32 pm Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL24] MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 1873 Kalim, > Can anyone tell me, for which tRNA species the crystal structures been > elucidated for (apart from tRNAphe from yeast). There are structures of initiator tRNAs from yeast (Paul Sigler group) and E. coli (Alexander Rich group). I have those refs handy: Schevitz et al., Nature 278:188, 1979 Woo et al., Nature 286:346, 1980 Basavappa et al., EMBO 10:3105, 1991 Dino Moras' group did free tRNAAsp. Lee and Fresco had very low-res data on tRNAGly in the early 80s; I don't know if that ever reached the literature. Any other free tRNAs, anyone? There are structures of synthetases complexed with tRNAs from various species: Glu (Tom Steitz group), Asp (Dino Moras group) and Ser (Steve Cusack group). They may or may not be helpful depending on what you want to learn from them. Current Opinion in Structural Biology 5:48, 1995 will get you started on those refs. > Know of any commercial (or other) supplies of these species? Subriden RNA PO Box 121 Rollingbay, WI 98061 206-842-8746 206-685-2582 Caveat: it's been some time since I contacted them myself. > Also are there any RNA structures (eg group 1 introns) whose crystal > structures have been elucidated but not published yet? Poul Nissen solved the complex of EF-Tu and tRNAPhe; it was presented at the ribosome meeting in the spring but I don't believe it's out yet. The teaser is in FEBS Letters 356(2-3):165-8, 1994 Dec 19. Udo Heinemann is working on the C-terminal domain of IF3 complexed to tRNAfMet. I don't know how far they've gotten. Why do you want to know? Cheers, -- | Dave Schweisguth For purposes of complying with | | dcs@proton.chem.yale.edu (MIME OK) the New Jersey Right to Know Act: | | http://proton.chem.yale.edu/~dcs/ Contents partially unknown. | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 | From BIOSCI-REQUEST Mon Oct 30 09:02:33 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA13362 for rna-list; Mon, 30 Oct 1995 09:02:33 -0800 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.12/8.6.6) with ESMTP id JAA13354 for ; Mon, 30 Oct 1995 09:02:14 -0800 Received: by proton.chem.yale.edu (950511.SGI.8.6.12.PATCH526/940406.SGI.AUTO) id MAA12054; Mon, 30 Oct 1995 12:00:58 -0500 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <199510301700.MAA12054@proton.chem.yale.edu> Subject: Re: crystal structures To: kalim@arl.rdc.toshiba.co.jp, rna@net.bio.net Date: Mon, 30 Oct 1995 12:00:58 -0500 (EST) In-Reply-To: from "dcs" at Oct 30, 95 08:44:20 am Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL24] MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 506 A moment ago I said: > Udo Heinemann is working on the C-terminal domain of IF3 complexed to > tRNAfMet. I don't know how far they've gotten. Ooops; that's IF3, not IF2. I was projecting. -- | Dave Schweisguth For purposes of complying with | | dcs@proton.chem.yale.edu (MIME OK) the New Jersey Right to Know Act: | | http://proton.chem.yale.edu/~dcs/ Contents partially unknown. | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 | From BIOSCI-REQUEST Mon Oct 30 13:25:31 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA20771 for rna-list; Mon, 30 Oct 1995 13:25:31 -0800 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.12/8.6.6) with ESMTP id LAA16952 for ; Mon, 30 Oct 1995 11:01:47 -0800 Received: by proton.chem.yale.edu (950511.SGI.8.6.12.PATCH526/940406.SGI.AUTO) id OAA18338; Mon, 30 Oct 1995 14:00:25 -0500 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <199510301900.OAA18338@proton.chem.yale.edu> Subject: Re: crystal structures To: kalim@arl.rdc.toshiba.co.jp, rna@net.bio.net Date: Mon, 30 Oct 1995 14:00:24 -0500 (EST) In-Reply-To: <199510301700.MAA12054@proton.chem.yale.edu> from "Dave Schweisguth" at Oct 30, 95 12:00:58 pm Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL24] MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 563 A moment ago I said > A moment ago I said: > > Udo Heinemann is working on the C-terminal domain of IF3 complexed to > > tRNAfMet. I don't know how far they've gotten. > > Ooops; that's IF3, not IF2. I was projecting. Aaargh! IF*2*, not IF*3*. -- | Dave Schweisguth For purposes of complying with | | dcs@proton.chem.yale.edu (MIME OK) the New Jersey Right to Know Act: | | http://proton.chem.yale.edu/~dcs/ Contents partially unknown. | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 |