From BIOSCI-REQUEST Fri Dec 1 06:49:32 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id GAA10891; Fri, 1 Dec 1995 06:49:32 -0800 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.12/8.6.6) with ESMTP id GAA10888 for ; Fri, 1 Dec 1995 06:49:27 -0800 Received: by proton.chem.yale.edu (950911.SGI.8.6.12.PATCH825/940406.SGI.AUTO) for rna@net.bio.net id JAA10082; Fri, 1 Dec 1995 09:48:02 -0500 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <199512011448.JAA10082@proton.chem.yale.edu> Subject: Re: t-rna To: rna@net.bio.net (RNA mailing list) Date: Fri, 1 Dec 1995 09:48:00 -0500 (EST) In-Reply-To: from "Peter Palenchar" at Dec 1, 95 00:10:38 am Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL24] MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 792 Peter Palenchar asks: > Does anybody know a good current source for t-RNA sequence and > tertiary structures? Nucleic Acids Research publishes a special issue cataloging sequence databases every two years. Sprinzl et al.'s tRNA database is in it. The articles reference online sources. Try ftp.embl-heidelberg.de. As for structures, Francis Ouelette recently posted a list of the tRNA structures in the PDB to this group. Like all Biosci groups, it's archived. Cheers, -- | Dave Schweisguth For purposes of complying with | | dcs@proton.chem.yale.edu (MIME OK) the New Jersey Right to Know Act: | | http://proton.chem.yale.edu/~dcs/ Contents partially unknown. | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 | From BIOSCI-REQUEST Tue Dec 5 03:08:37 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id DAA29514; Tue, 5 Dec 1995 03:08:37 -0800 Received: from ntumc1.mc.ntu.edu.tw (ntumc1.mc.ntu.edu.tw [140.112.131.1]) by net.bio.net (8.6.12/8.6.6) with SMTP id DAA29507; Tue, 5 Dec 1995 03:08:27 -0800 Received: by ntumc1.mc.ntu.edu.tw (5.57/DEC-Ultrix/4.3) id AA19558; Tue, 5 Dec 95 19:03:35 +0800 Date: Tue, 5 Dec 1995 19:03:34 +0800 (WST) From: Fung-Jou Lu To: rna@net.bio.net Subject: how can I join your net.bio.net Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII you can tell me how to join the net.bio.net I am a graduate student at National Taiwan University So far, I study biochemistry in Department of Biochemistry. I research about RNA level for the toxicity(Humic acid). And to know how it affect the RNA level. I would be glad to see the information from the net.bio.net From BIOSCI-REQUEST Tue Dec 5 10:41:14 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id KAA21408; Tue, 5 Dec 1995 10:41:14 -0800 Received: from electra.cc.umanitoba.ca (root@electra.cc.umanitoba.ca [130.179.16.23]) by net.bio.net (8.6.12/8.6.6) with ESMTP id KAA21405; Tue, 5 Dec 1995 10:41:10 -0800 Received: from adminbldg.lan1.umanitoba.ca (smtp.lan1.umanitoba.ca [130.179.16.43]) by electra.cc.umanitoba.ca (8.7.1/8.7.1) with SMTP id MAA17767 for ; Tue, 5 Dec 1995 12:39:39 -0600 (CST) Received: by adminbldg.lan1.umanitoba.ca with Microsoft Mail id <30C491B4@adminbldg.lan1.umanitoba.ca>; Tue, 05 Dec 95 12:38:44 CST From: "Huebnerlab, Erwin" To: rna@net.bio.net Subject: An RNA question... Date: Tue, 05 Dec 95 12:33:00 CST Message-ID: <30C491B4@adminbldg.lan1.umanitoba.ca> Encoding: 12 TEXT X-Mailer: Microsoft Mail V3.0 I am a student at the University of Manitoba with a question about isolating RNA, for anyone who can spare the time...i have recently isolated RNA from the ovaries of the insect Rhodnius prolixus using TRIZOL from Gibco; the product contains a strong 18S rRNA band, but the 26S rRNA band is absent. I have been schooled to believe that if these bands are intact (which they are, sort of), one can be reasonably confident that they have good quality RNA. Has anyone else run into this before? Is there any other simple (and inexpensive) way to test RNA integrity? I would greatly appreciate any feedback! Chris Bjornsson From BIOSCI-REQUEST Sat Dec 9 02:00:20 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA19740; Sat, 9 Dec 1995 02:00:20 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA19718; Sat, 9 Dec 1995 02:00:11 -0800 Date: Sat, 9 Dec 1995 02:00:11 -0800 From: BIOSCI Administrator Message-Id: <199512091000.CAA19718@net.bio.net> To: rna@net.bio.net Subject: IMPORTANT: BIOSCI miniFAQ (LAST REVISION: 08-DEC-95) This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. We are working on new systems to provide access to our newsgroups over the WWW. These will be available by 11 December 1995 and will allow you to use your Web browser to look at the news postings via our Web site at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Fri Dec 22 04:44:15 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA06191; Fri, 22 Dec 1995 04:44:15 -0800 Received: from ncbi.nlm.nih.gov (ncbi.nlm.nih.gov [130.14.25.1]) by net.bio.net (8.6.12/8.6.6) with SMTP id EAA06187; Fri, 22 Dec 1995 04:44:10 -0800 Received: from borduas.nlm.nih.gov by ncbi.nlm.nih.gov id AA18452; Fri, 22 Dec 1995 07:42:23 -0500 Received: by borduas.nlm.nih.gov id AA17897; Fri, 22 Dec 1995 07:42:22 -0500 Date: Fri, 22 Dec 1995 07:42:22 -0500 Message-Id: <9512221242.AA17897@borduas.nlm.nih.gov> To: rna@net.bio.net, JBEGGS@srv0.bio.ed.ac.uk Subject: Re: RNA 96 Meeting From: francis@ncbi.nlm.nih.gov X-Sun-Charset: US-ASCII I'm forwarding this to the rna mailing list ... happy holidays to all, francis -- | B.F. Francis Ouellette | GenBank | | francis@ncbi.nlm.nih.gov ----- Begin Included Message ----- >From BIOSCI-REQUEST@net.bio.net Fri Dec 22 06:09 EST 1995 To: yeast@net.bio.net From: JBEGGS@srv0.bio.ed.ac.uk (Jean Beggs) Subject: RNA 96 Meeting Date: 22 Dec 1995 02:59:58 -0800 Nntp-Posting-Host: net.bio.net The First Annual Meeting of the RNA Society will take place from 28th May to 2nd June 1996 at the University of Madison-Wisconsin. The topics to be covered include virtually all posttranscriptional aspects of RNA. The deadline for registration is 22nd February 1996. Full details and registration materials can be obtained on Internet as follows: USA site: http://www.cup.org/Journals/RNA/classified/rnameet.html UK/Europe: http://www.cup.cam.ac.uk/Journals/RNA/classified/rnameet.html Professor Jean D. Beggs Institute of Cell and Molecular Biology University of Edinburgh King's Buildings Mayfield Road Edinburgh EH9 3JR UK Tel: 44 131 650 5351 Fax: 44 131 668 3870 ----- End Included Message ----- From BIOSCI-REQUEST Mon Dec 25 21:09:05 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id VAA12855; Mon, 25 Dec 1995 21:09:05 -0800 Received: from jhuml1.hcf.jhu.edu (jhuml1.hcf.jhu.edu [128.220.2.86]) by net.bio.net (8.6.12/8.6.6) with ESMTP id VAA12852; Mon, 25 Dec 1995 21:09:02 -0800 Received: from dediris.chm.jhu.edu by jhmail.hcf.jhu.edu (PMDF V4.3-9 #5488) id <01HZ8RMJ83SG921ZLU@jhmail.hcf.jhu.edu>; Tue, 26 Dec 1995 00:07:22 -0400 (EDT) Received: from dediris.chm.jhu.edu by jhmail.hcf.jhu.edu (PMDF V4.3-9 #5488) id <01HZ8RMICJ34921ZJ6@jhmail.hcf.jhu.edu>; Tue, 26 Dec 1995 00:07:21 -0400 (EDT) Received: by dediris.chm.jhu.edu; Mon, 25 Dec 1995 23:33:13 -0500 Date: Mon, 25 Dec 1995 23:33:13 -0500 From: sh@dediris.chm.jhu.edu (Shengrong Huang) Sender: sh@dediris.chm.jhu.edu Apparently-to: rna@net.bio.net To: rna@net.bio.net Message-id: <01HZ8RMICVC2921ZJ6@jhmail.hcf.jhu.edu> Content-transfer-encoding: 7BIT Are there any crystal structures of RNA (including oligonucleotides) and RNA-protein complexes published in 1995 ? A grad student interested in progress made by crystallographer. From BIOSCI-REQUEST Mon Dec 25 21:13:24 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id VAA13040; Mon, 25 Dec 1995 21:13:24 -0800 Received: from jhuml1.hcf.jhu.edu (jhuml1.hcf.jhu.edu [128.220.2.86]) by net.bio.net (8.6.12/8.6.6) with ESMTP id VAA13037; Mon, 25 Dec 1995 21:13:23 -0800 Received: from dediris.chm.jhu.edu by jhmail.hcf.jhu.edu (PMDF V4.3-9 #5488) id <01HZ8RRWZ4M8921ZLU@jhmail.hcf.jhu.edu>; Tue, 26 Dec 1995 00:11:42 -0400 (EDT) Received: from dediris.chm.jhu.edu by jhmail.hcf.jhu.edu (PMDF V4.3-9 #5488) id <01HZ8RRVQIS091YHPO@jhmail.hcf.jhu.edu>; Tue, 26 Dec 1995 00:11:41 -0400 (EDT) Received: by dediris.chm.jhu.edu; Mon, 25 Dec 1995 23:51:35 -0500 Date: Mon, 25 Dec 1995 23:51:35 -0500 From: sh@dediris.chm.jhu.edu (Shengrong Huang) Sender: sh@dediris.chm.jhu.edu Apparently-to: rna@net.bio.net To: rna@net.bio.net Message-id: <01HZ8RRVQVYQ91YHPO@jhmail.hcf.jhu.edu> Content-transfer-encoding: 7BIT Are there any crystal structures of RNA (including oligonucleotides) and RNA-protein complexes published in 1995 ? A grad student interested in progress made by crystallographers.