From BIOSCI-REQUEST Thu Feb 1 09:02:46 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA22799; Thu, 1 Feb 1996 09:02:46 -0800 Received: from aaRS.mit.edu (AARS.MIT.EDU [18.79.0.30]) by net.bio.net (8.6.12/8.6.6) with ESMTP id JAA22795; Thu, 1 Feb 1996 09:02:36 -0800 Received: from schimmel (SCHIMMEL.MIT.EDU [18.79.0.31]) by aaRS.mit.edu (8.7.1/8.7.1) with SMTP id MAA08437 for ; Thu, 1 Feb 1996 12:01:32 -0500 (EST) Message-Id: <2.2.32.19960201170155.002cfa40@aars.mit.edu> X-Sender: barry@aars.mit.edu X-Mailer: Windows Eudora Pro Version 2.2 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 01 Feb 1996 12:01:55 -0500 To: rna@net.bio.net From: Barry Henderson Subject: Extracting thermodynamic parameters from melting curves Hi- I need to extract enthalpy, entropy and free energy values from thermal denaturation curves of small RNAs. The melts were generated on a Cary 3 but the software that comes along with the instrument is marginal at best for conducting this type of analysis. Is there any software available that will assist me in this type of analysis. Thanks, Barry Barry Henderson Postdoctoral Fellow MIT Department of Biology (617) 253-0808 (Voice) (617) 253-6636 (FAX) From BIOSCI-REQUEST Thu Feb 1 09:45:39 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA27446; Thu, 1 Feb 1996 09:45:39 -0800 Received: from proton.chem.yale.edu (proton.chem.yale.edu [130.132.25.95]) by net.bio.net (8.6.12/8.6.6) with ESMTP id JAA27427; Thu, 1 Feb 1996 09:45:31 -0800 Received: by proton.chem.yale.edu (951211.SGI.8.6.12.PATCH1042/951211.SGI.AUTO) for rna@net.bio.net id MAA17502; Thu, 1 Feb 1996 12:43:26 -0500 From: dcs@proton.chem.yale.edu (Dave Schweisguth) Message-Id: <199602011743.MAA17502@proton.chem.yale.edu> Subject: Re: Extracting thermodynamic parameters from melting curves To: rna@net.bio.net (RNA mailing list) Date: Thu, 1 Feb 1996 12:43:25 -0500 (EST) In-Reply-To: <2.2.32.19960201170155.002cfa40@aars.mit.edu> from "Barry Henderson" at Feb 1, 96 12:01:55 pm Organization: Dept. of Chemistry, Yale University X-Mailer: ELM [version 2.4 PL25] MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 939 Barry, > I need to extract enthalpy, entropy and free energy values from thermal > denaturation curves of small RNAs. The melts were generated on a Cary 3 > but the software that comes along with the instrument is marginal at best > for conducting this type of analysis. Is there any software available that > will assist me in this type of analysis. Ask David Draper for a copy of his MeltFit software for Macintoshes. Alternatively, Fudgit (FTPable from ftp.physics.mcgill.edu) is a nice general curve-fitting package with which you could handle melt data and many other things. Cheers, -- | Dave Schweisguth For purposes of complying with | | dcs@proton.chem.yale.edu (MIME OK) the New Jersey Right to Know Act: | | http://proton.chem.yale.edu/~dcs/ Contents partially unknown. | | Yale Depts. of MB&B & Chemistry Phone: 203-432-5208 Fax: 203-432-6144 | From BIOSCI-REQUEST Thu Feb 1 13:29:30 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA25768; Thu, 1 Feb 1996 13:29:30 -0800 Received: from cifn.unam.mx (uxmal.cifn.unam.mx [132.248.34.3]) by net.bio.net (8.6.12/8.6.6) with SMTP id NAA25758; Thu, 1 Feb 1996 13:29:17 -0800 Received: by cifn.unam.mx (5.0/SMI-SVR4) id AA09856; Thu, 1 Feb 1996 15:19:56 +0600 Date: Thu, 1 Feb 1996 15:19:56 -0600 (CST) From: Ernesto Perez X-Sender: ernesto@uxmal To: rna@net.bio.net Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII content-length: 37 subscribe RNA ernesto@cifn.unam.mx From BIOSCI-REQUEST Wed Feb 7 09:37:48 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA15811; Wed, 7 Feb 1996 09:37:48 -0800 Received: from agc3.agr.ca (gw.agr.ca [192.197.71.131]) by net.bio.net (8.6.12/8.6.6) with ESMTP id JAA15800; Wed, 7 Feb 1996 09:37:43 -0800 From: CHISHOLM@bcrssu.agr.ca Received: from gateway.ncr.agr.ca (agc6.agr.ca [192.197.71.68]) by agc3.agr.ca (8.7.1/8.7.1) with ESMTP id MAA07417 for ; Wed, 7 Feb 1996 12:33:53 -0500 (EST) Received: from VMGW.AGR.CA ([142.61.33.3]) by gateway.ncr.agr.ca (8.7.1/8.7.1) with SMTP id MAA15222 for <@gateway.ncr.agr.ca:rna@net.bio.net>; Wed, 7 Feb 1996 12:32:20 -0500 (EST) Date: 07 Feb 1996 12:28:37 -0400 (EDT) Subject: cDNA from total RNA To: rna Message-id: <01I0XJXI8OIQ000KKN@VMGW.AGR.CA> X-VMS-To: GW"rna@net.bio.net" MIME-version: 1.0 Content-transfer-encoding: 7BIT Does anyone have a protocol for synthesizing cDNAs from total RNA? I'm interested in making a cDNA library and have not had much success going through an oligodT column. I'm not sure about the polyA tails on the RNA (it's from fruit). Thanks J Chisholm From BIOSCI-REQUEST Fri Feb 9 02:01:03 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA08005; Fri, 9 Feb 1996 02:01:03 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA07974; Fri, 9 Feb 1996 02:00:26 -0800 Date: Fri, 9 Feb 1996 02:00:26 -0800 From: BIOSCI Administrator Message-Id: <199602091000.CAA07974@net.bio.net> To: rna@net.bio.net Subject: BIOSCI miniFAQ, ver. 14-DEC-95 (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Mon Feb 12 11:22:20 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id LAA22435; Mon, 12 Feb 1996 11:22:20 -0800 Received: from mta3.nts.uci.edu (mta3.nts.uci.edu [128.200.220.2]) by net.bio.net (8.6.12/8.6.6) with SMTP id LAA22422 Received: by mta3.nts.uci.edu id AA25066 (5.67b/IDA-1.4.4 for plantbio@net.bio.net); Mon, 12 Feb 1996 11:22:54 -0800 Received: from gandalf.bio.uci.edu by mta3.nts.uci.edu with SMTP id AA25047 (5.67b/IDA-1.4.4 for plantbio@net.bio.net); Mon, 12 Feb 1996 11:22:49 -0800 Received: from ccMail by gandalf.bio.uci.edu id AA824152760 Mon, 12 Feb 96 11:19:20 PST Date: Mon, 12 Feb 96 11:19:20 PST From: "Michael R. MULLIGAN" Encoding: 1657 Text Message-Id: <9601128241.AA824152760@gandalf.bio.uci.edu> To: arab-gen@net.bio.net, chlamy@net.bio.net, photosyn@net.bio.net, rna@net.bio.net, plantbio@net.bio.net Subject: re: post-doctoral position POSTDOCTORAL POSITION IN RNA EDITING IN PLANTS We are interested in RNA editing in plant mitochondria and chloroplasts. RNA editing is a post-transcriptional process that converts cytidines to uridines, and consequently changes the amino acid specified by a codon. As a result, the DNA encodes genes with "mistakes" that are corrected post-transcriptionally by editing. My lab is funded for two projects- one project addresses the consequences of incomplete editing. Some populations of transcripts are highly heterogeneous as a result of incomplete editing. We have recently shown (Plant Cell 8:107) that both unedited and edited ribosomal protein subunit 12 mRNAs are translated, but only the edited translation products assemble into ribosomes. The incompletely edited translation products, which have aberrant amino acid residues, fail to accumulate as ribosomal subunits and are free in the matrix. A second project in the lab addresses mechanistic aspects of the editing process. Funding for a post-doctoral scientist is available through grants from the USDA and Human Frontiers Science Program. Applicants should send their resume, statement of research interests, and names and addresses of three referees to: Dr. R. Michael Mulligan Department of Developmental and Cell Biology University of California Irvine, CA 92717 **** Located six miles from the ocean in Orange County, UCI is in the center of a flourishing high technology and cultural center. The campus is very strong in biomedical research. We are an equal opportunity employer committed to excellence through diversity. From BIOSCI-REQUEST Mon Feb 12 12:58:37 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id MAA04991; Mon, 12 Feb 1996 12:58:37 -0800 Received: from agc3.agr.ca (gw.agr.ca [192.197.71.131]) by net.bio.net (8.6.12/8.6.6) with ESMTP id MAA04988; Mon, 12 Feb 1996 12:58:35 -0800 Received: from gateway.ncr.agr.ca (agc6.agr.ca [192.197.71.68]) by agc3.agr.ca (8.7.1/8.7.1) with ESMTP id PAA22836 for ; Mon, 12 Feb 1996 15:54:43 -0500 (EST) Received: from EM.AGR.CA ([142.61.33.4]) by gateway.ncr.agr.ca (8.7.1/8.7.1) with SMTP id PAA10822 for ; Mon, 12 Feb 1996 15:53:06 -0500 (EST) Received: from GW1-Message_Server by EM.AGR.CA with Novell_GroupWise; Mon, 12 Feb 1996 15:43:20 -0500 Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Mon, 12 Feb 1996 14:05:10 -0500 From: Les Willis To: rna@net.bio.net Subject: Optimizing long transcription reactions Dear Netters: I would appreciate suggestions on optimizing a transcription reaction with a very long (19.3 kB) template. Yes, that's 19.3 (not a typo). I've been able to get the full length product but I also produce a fair amount of smearing (above and below the band). The protocol I'm currently using is as follows: 10 ul ds DNA template (2ug) 10 ul Promega 10X Transcription Buffer 5 ul 100 mM DTT 1 ul RNaisin (Promega 40 U/ul) 3 ul T7 RNA Polymerase (Promega 20 U/ul) 2.5ul each rNTP 10 mM stock of each (Promega) (10 ul total) 18.5 ul dH20 --------- 50 ul total Overlay with mineral oil and place at 37 C for 1 hour 1ul RQ DNase (Promega 1U/ ul) added, 37 C for another 30 minutes Phenol/ Chloroform; Ppt with NH4Ac (2.5 M final conc.) and 3 vol EtOH Any tips on how to increase my yield of full length transcript would be appreciated. Thanks in advance. Les. Les Willis Agriculture Canada Summerland, B.C. WillisL@em.agr.ca From BIOSCI-REQUEST Tue Feb 13 13:37:41 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA12388; Tue, 13 Feb 1996 13:37:41 -0800 Received: from agc3.agr.ca (gw.agr.ca [192.197.71.131]) by net.bio.net (8.6.12/8.6.6) with ESMTP id NAA12385; Tue, 13 Feb 1996 13:37:39 -0800 Received: from gateway.ncr.agr.ca (agc6.agr.ca [192.197.71.68]) by agc3.agr.ca (8.7.1/8.7.1) with ESMTP id QAA28518 for ; Tue, 13 Feb 1996 16:33:46 -0500 (EST) Received: from EM.AGR.CA ([142.61.33.4]) by gateway.ncr.agr.ca (8.7.1/8.7.1) with SMTP id QAA02367 for ; Tue, 13 Feb 1996 16:32:08 -0500 (EST) Received: from GW1-Message_Server by EM.AGR.CA with Novell_GroupWise; Tue, 13 Feb 1996 16:30:03 -0500 Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Tue, 13 Feb 1996 16:33:36 -0500 From: Les Willis To: rna@net.bio.net Subject: Re:Optimizing Long Transcription Reactions - responses Hello Everyone: Thanks to those of you who responded to my query about optimizing long transcription reactions. There were numerous suggestions including incubating at 42C, increasing rNTP conc., lower the amount of template, polishing the template ends, using DMSO, extending the reaction time to 4-6 hours, using DNA binding proteins, stabilizing with BSA, etc. I plan to do tests to explore a number of these suggestions. I also have deletions of the 19.3 kB template (17, 15, 13, 11kB) which will also (hopefully) be tested. I'll let everyone know what I find works best. Thanks again for the help. Les. Les Willis Agriculture Canada Summerland, B.C. WillisL@em.agr.ca From BIOSCI-REQUEST Thu Feb 15 15:22:46 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA02663; Thu, 15 Feb 1996 15:22:46 -0800 Received: from CPWSCA.PSC.EDU (a.psc.edu [128.182.66.105]) by net.bio.net (8.6.12/8.6.6) with SMTP id PAA02656; Thu, 15 Feb 1996 15:22:39 -0800 Date: Thu, 15 Feb 1996 18:21:20 -0500 (EST) From: HECKATHO@B.PSC.EDU To: rna@net.bio.net Message-Id: <960215182120.20402d62@B.PSC.EDU> Subject: Pittsburgh Supercomputing Center 1996 Biomed Workshops ************************************************************************** PITTSBURGH SUPERCOMPUTING CENTER BIOMEDICAL SUPERCOMPUTING INITIATIVE Biomedical Workshops offered by the Pittsburgh Supercomputing Center typically consist of theoretical lectures taught by leaders in the respective scientific discipline, and extensive hands-on computer sessions. During the computer sessions, participants are able to work on the examples provided or on their own experimental data. Attendance is limited to 20 participants to allow one-on-one instruction and encourage scientific interactions and discussions. Application deadlines are six weeks prior to the workshop. Researchers nationwide are invited to apply. For additional information, please refer to http://www.psc.edu/biomed/workshops.html CONTACT INFORMATION: Nancy Blankenstein, Biomedical Program Assistant, (412)268-4960, blankens@psc.edu TO APPLY FOR A WORKSHOP, please fill out the application form. The following four workshops will be offered during 1996: SUPERCOMPUTING TECHNIQUES FOR BIOMEDICAL RESEARCHERS; May 5-9. The purpose of this workshop is to introduce attendees to the concepts of supercomputing. The main goal is to provide researchers with a firm basis from which to analyze their applications for implementation in a supercomputing environment. After a brief introduction to PSC's supercomputing environment, including the architectures of the CRAY C90 and T3E, various vector and massively parallel programming models will be discussed. Message passing, performance monitoring, optimization techniques, and heterogeneous supercomputing will be introduced with emphasis on practical considerations. Specific real-world biomedical supercomputing applications will be presented to illustrate these concepts. Finally, a panel discussion will attempt to address researchers' individual application questions. NUCLEIC ACID AND PROTEIN SEQUENCE ANALYSIS; June 9-14. Emphasis will be on alignment of and pattern extraction from multiple sequences. Topics to be discussed include Comparing and aligning sequences Identifying informative patterns in a set of sequences Using extracted informative patterns to identify related sequences. Leaders are Dr. Gary Churchill, Cornell University; Dr. Michael Gribskov, San Diego Supercomputing Center and Dr. Hugh B. Nicholas, Jr., Pittsburgh Supercomputing Center. METHODS AND APPLICATIONS OF MOLECULAR MECHANICS AND DYNAMICS TO MOLECULES OF BIOLOGICAL INTEREST; August 7-10. Instructors include Dr. David Case, the Scripps Research Institute, Thomas Cheatham III, UCSF; Prof. Peter A. Kollman, UCSF, Dr. David A. Pearlman, Vertex Pharmaceuticals; and Bill Ross, UCSF. General aspects of molecular mechanics and dynamics theory and software will be discussed. The program AMBER will be utilized extensively in demonstrations. ADVANCED NUCLEIC ACID AND PROTEIN SEQUENCE ANALYSIS; Dates TBA; subject to funding availability. Open to researchers who have previously attended one of the PSC's annual "Nucleic Acid and Protein Sequence Analysis" workshops or who have appreciable experience with computerized sequence analysis. The workshop will build on previous experience to teach techniques for analyzing families and superfamilies of genes and proteins. Please fill out the following application form. APPLICATION FORM - Biomedical Workshop Application Deadline: six weeks prior to each workshop. PITTSBURGH SUPERCOMPUTING CENTER BIOMEDICAL SUPERCOMPUTING INITIATIVE WORKSHOP APPLICATION FORM Workshop I am interested in attending:_____________________________________ Name: ________________________________________________________________ Affiliation: ________________________________________________________________ Address: ________________________________________________________________ (Business) ________________________________________________________________ ________________________________________________________________ (Home) ________________________________________________________________ Telephone: ____________________________ ______________________________ (Business) (Home) *Social Security Number: _______-_____-_______ Citizenship:___________________ Electronic Mail Address:_______________________________________________________ Status: ___Graduate ___Post-doctoral Fellow ___Faculty ___Other (specify) Please indicate specifically any special housing, transportation or dietary arrangements you will need: ___________________________________________________ How did you learn about this workshop:_________________________________________ REQUIREMENTS: Applicants must submit a completed application form and a cover letter. The letter should describe, in one or two paragraphs, your current research, and how participating in the workshop will enhance this research. Please include a brief statement describing your level of experience with computers. Faculty members, staff and post-docs should provide a curriculum vitae. Graduate students must have a letter of recommendation from a faculty member. Please return all application materials six weeks before the workshop you are applying for to: Biomedical Workshop Applications Committee Pittsburgh Supercomputing Center 4400 Fifth Avenue, Suite 230C Pittsburgh, PA 15213 *Disclosure of Social Security Number is voluntary. PSC does not discriminate on the basis of race, color, religion, sex, age, creed, national or ethnic origin, or handicap. From BIOSCI-REQUEST Wed Feb 21 14:11:41 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id OAA04697; Wed, 21 Feb 1996 14:11:41 -0800 Received: from nj2.n-jcenter.com (nj2.n-jcenter.com [205.160.185.3]) by net.bio.net (8.6.12/8.6.6) with SMTP id OAA04692; Wed, 21 Feb 1996 14:11:36 -0800 Received: from flpm1_06.n-jcenter.com by nj2.n-jcenter.com with SMTP (5.65/1.2-eef) id AA26618; Wed, 21 Feb 96 17:06:46 -0500 Date: Wed, 21 Feb 96 17:06:46 -0500 Message-Id: <9602212206.AA26618@nj2.n-jcenter.com> X-Sender: gerardo@n-jcenter.com X-Mailer: Windows Eudora Version 1.4.5 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: rna@net.bio.net From: gerardo@n-jcenter.com (Gerardo Medrano) Subject: subscribe "Si pequena es la Patria, uno grande la suena..." Ruben Dario From BIOSCI-REQUEST Fri Feb 23 12:38:15 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id MAA21396; Fri, 23 Feb 1996 12:38:15 -0800 Received: from nj2.n-jcenter.com (nj2.n-jcenter.com [205.160.185.3]) by net.bio.net (8.6.12/8.6.6) with SMTP id MAA21392; Fri, 23 Feb 1996 12:38:11 -0800 Received: from flpm1_14.n-jcenter.com by nj2.n-jcenter.com with SMTP (5.65/1.2-eef) id AA23338; Fri, 23 Feb 96 15:33:35 -0500 Date: Fri, 23 Feb 96 15:33:35 -0500 Message-Id: <9602232033.AA23338@nj2.n-jcenter.com> X-Sender: gerardo@n-jcenter.com X-Mailer: Windows Eudora Version 1.4.5 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: rna@net.bio.net From: gerardo@n-jcenter.com (Gerardo Medrano) Subscribe End "Si pequena es la Patria, uno grande la suena..." Ruben Dario