From BIOSCI-REQUEST Tue Jul 9 02:00:08 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA23147; Tue, 9 Jul 1996 02:00:08 -0700 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA23127; Tue, 9 Jul 1996 02:00:04 -0700 Date: Tue, 9 Jul 1996 02:00:04 -0700 From: BIOSCI Administrator Message-Id: <199607090900.CAA23127@net.bio.net> To: rna@net.bio.net Subject: IMPORTANT - BIOSCI Fundraising Update! BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From BIOSCI-REQUEST Sat Jul 13 02:29:02 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA04985; Sat, 13 Jul 1996 02:29:02 -0700 Received: from po8.andrew.cmu.edu (PO8.ANDREW.CMU.EDU [128.2.10.108]) by net.bio.net (8.6.12/8.6.6) with ESMTP id CAA04981; Sat, 13 Jul 1996 02:28:59 -0700 Received: (from postman@localhost) by po8.andrew.cmu.edu (8.7.5/8.7.3) id FAA17792; Sat, 13 Jul 1996 05:29:01 -0400 Received: via switchmail; Sat, 13 Jul 1996 05:29:00 -0400 (EDT) Received: from po6.andrew.cmu.edu via qmail ID ; Sat, 13 Jul 1996 05:28:17 -0400 (EDT) Received: from unix14.andrew.cmu.edu via qmail ID ; Fri, 12 Jul 1996 13:45:46 -0400 (EDT) Received: from mms.4.60.Jun.27.1996.03.02.53.sun4.51.EzMail.2.0.CUILIB.3.45.SNAP.NOT.LINKED.unix14.andrew.cmu.edu.sun4m.54 via MS.5.6.unix14.andrew.cmu.edu.sun4_51; Fri, 12 Jul 1996 13:45:46 -0400 (EDT) Message-ID: <8ltcx_K00YUq0EM1E0@andrew.cmu.edu> Date: Fri, 12 Jul 1996 13:45:46 -0400 (EDT) From: Alexander J Ropelewski To: Outbound News Subject: CCL:biomedical workshop announcement Cc: Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , Outbound News , chemistry@osc.edu, rna@net.bio.net, amber@cgl.ucsf.edu, Outbound News , carol@newsmaster.tgc.com BIOMEDICAL WORKSHOPS PITTSBURGH SUPERCOMPUTING CENTER August 25-28, 1996 The Pittsburgh Supercomputing Center is offering an "ADVANCED NUCLEIC ACID AND PROTEIN SEQUENCE ANALYSIS" workshop. The workshop is open to researchers who have previously attended one of the PSC's annual "Nucleic Acid and Protein Sequence Analysis" workshops or who have previous experience with computerized sequence analysis. The workshop will build on previous experience to teach techniques for what is commonly referred to as "homology modeling", specifically the methods known as threading techniques. This involves fitting the sequences of proteins whose structure is not known to the known three-dimensional structure of another protein. The workshop will feature the threading techniques developed by Dr. Stephen H. Bryant of the Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine. Dr. Bryant's work focuses on empirical energy functions that measure the complementarity of sequence and folding motif and on algorithms for rapid threading. His group is also developing computer programs for automatically creating homology models derived from core structural motifs identified by other sequence analysis techniques. Instructors are: Dr. Stephen H. Bryant, National Center for Biotechnology Information, National Library of Medicine Dr. Michael Gribskov, San Diego Supercomputer Center and Dr. Hugh B. Nicholas Jr., Pittsburgh Supercomputing Center. APPLICATION DEADLINE: July 17, 1996. For additional information, please refer to http://www.psc.edu/biomed/workshops.html CONTACT INFORMATION: Nancy Blankenstein, Biomedical Program Assistant, (412)268-4960, blankens@psc.edu ********** PITTSBURGH SUPERCOMPUTING CENTER ADVANCED SEQUENCE ANALYSIS WORKSHOP APPLICATION Name: ________________________________________________________________ Affiliation: ________________________________________________________________ Address: ________________________________________________________________ (Business) ________________________________________________________________ ________________________________________________________________ (Home) ________________________________________________________________ Telephone: ____________________________ ______________________________ (Business) (Home) *Social Security Number: _______-_____-_______ Citizenship:___________________ Electronic Mail Address:_______________________________________________________ Status: ___Graduate ___Post-doctoral Fellow ___Faculty ___Other (specify) Please indicate specifically any special housing, transportation or dietary arrangements you will need: __________________________________________ How did you learn about this workshop:_________________________________________ REQUIREMENTS: Applicants must submit a completed application form and a cover letter. The letter should describe, in one or two paragraphs, the sequence analysis problems encountered in your research, and how participating in the workshop will enhance this research. Please include a brief statement describing your level of experience with computers. Faculty members, staff and post-docs should provide a curriculum vitae. Graduate students must have a letter of recommendation from a faculty member. Please return all application materials to: Biomedical Workshop Applications Committee Pittsburgh Supercomputing Center 4400 Fifth Avenue, Suite 230C Pittsburgh, PA 15213 Direct inquiries to: Nancy Blankenstein, blankens@psc.edu or 412/268-4960. *Disclosure of Social Security Number is voluntary. PSC does not discriminate on the basis of race, color, religion, sex, age, creed, national or ethnic origin, or handicap. From BIOSCI-REQUEST Fri Jul 19 00:03:14 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id AAA15337; Fri, 19 Jul 1996 00:03:14 -0700 Received: from sun1.biomed.cas.cz (sun1.biomed.cas.cz [147.231.40.1]) by net.bio.net (8.6.12/8.6.6) with SMTP id AAA15330; Fri, 19 Jul 1996 00:03:07 -0700 Received: from 147.231.43.68 (jforejt.biomed.cas.cz) by sun1.biomed.cas.cz (4.1/SMI-4.1) id AA22925; Fri, 19 Jul 96 09:02:32 +0200 Message-Id: <31EF41E1.CB8@biomed.cas.cz> Date: Fri, 19 Jul 1996 09:05:53 +0100 From: Jiri Forejt X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Mime-Version: 1.0 To: rna@net.bio.net Subject: PolyA addition X-Url: http://www.bio.net:80/hypermail/RNA/ Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Does anyone know how specific is the place of addition of PolyA tail to a transcript of a particular gene (its particular allele). For example, with a gene XY is the addition of polyA always 34 nt after polyA signal OR is it 34 plus minus 3 nt (or so). Thanks for any comments Jiri Forejt From BIOSCI-REQUEST Thu Jul 25 17:54:15 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id RAA27849; Thu, 25 Jul 1996 17:54:15 -0700 Received: from sangam.ncst.ernet.in (sangam.ncst.ernet.in [202.41.110.33]) by net.bio.net (8.6.12/8.6.6) with ESMTP id RAA27846; Thu, 25 Jul 1996 17:54:04 -0700 Received: (from uucp@localhost) by sangam.ncst.ernet.in (8.6.12/8.6.6) with UUCP id GAA02554 for rna@net.bio.net; Fri, 26 Jul 1996 06:27:43 +0530 Received: from dbeb.iitd.ernet.in (netearth) by henna.iitd.ernet.in (4.1/SMI-4.1-MHS-7.0 ) id AA09737; Thu, 25 Jul 96 16:46:04 IST X-Organisation: Indian Institute of Technology, New Delhi. Received: by dbeb.iitd.ernet.in; id AA21001; Thu, 25 Jul 1996 16:45:55 +0530 Received: by dbeb; Thu, 25 Jul 1996 16:45:55 +0530 id AA21001; Thu, 25 Jul 1996 16:45:55 +0530 Date: Thu, 25 Jul 1996 16:45:54 +0530 (IST) From: Sanjay Kumar Shahi Sender: Sanjay Kumar Shahi Reply-To: Sanjay Kumar Shahi Subject: ribozyme research To: rna@net.bio.net Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII I am working (for my graduate study i.e Ph.D.) on a project involving post-transcriptional gene regulation by targeted ribozyme. I would like to know if there is any group in Germany working on the projects related to the above i.e post-transcriptional gene regulation by targeted ribozyme, or some other related projects! Thanks and waiting for kind reply. S K Shahi