From BIOSCI-REQUEST Wed Nov 6 23:37:44 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id XAA19089; Wed, 6 Nov 1996 23:37:44 -0800 Received: from dfw-ix11.ix.netcom.com (dfw-ix11.ix.netcom.com [206.214.98.11]) by net.bio.net (8.6.12/8.6.6) with ESMTP id XAA19084; Wed, 6 Nov 1996 23:37:28 -0800 Received: from 204.30.69.130 (min-mn4-02.ix.netcom.com [204.30.69.130]) by dfw-ix11.ix.netcom.com (8.6.13/8.6.12) with SMTP id XAA03885 for ; Wed, 6 Nov 1996 23:37:33 -0800 Message-ID: <32819255.2554@ix.netcom.com> Date: Thu, 07 Nov 1996 01:40:05 -0600 From: Michael Ravnitzky Reply-To: MikeRav@ix.netcom.com X-Mailer: Mozilla 2.01E (Macintosh; U; PPC) MIME-Version: 1.0 To: rna@net.bio.net Subject: get a list of military technical reports on RNA and related topics X-URL: http://www.bio.net/hypermail/RNA/ Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit I thought you might find this an interesting government information resource, on subject[s] you or your associates are interested in. If not, I apologize. Could you forward it on to the right person? At a government agency called DTIC: Defense Technical Information Center, there are a large number of government technical reports on almost any subject under the sun. NOTE: Donıt bother using the DTIC web site--in a nutshell, itıs worthless and omits the bulk of the two million technical reports in the DTIC collection. Send a letter--youıll get much better results. You'll have to select your own keywords for the exact subjects youıre interested in. I suggest you select no more than ten keywords or less for each letter. Please select your keyword[s] carefully and include plural[s] like I just did. Put an *OR* between each keyword or subject term. The fee is likely to be free or only a few bucks. You probably want to include a statement in the letter such as *I agree to pay reasonable fees associated with this request. Please notify me if the cost will exceed $30.*, so that they won't delay the processing of the request. Remember, they will try to dissuade you from asking for such a list. If they send you a letter, and you donıt respond, they will withdraw your request and you wonıt get your information. To get your bibliography [list of technical reports], send a letter of request as follows: To: Defense Technical Information Center Attn: DTIC-RSM [Kelly D. Akers, FOIA Manager] 8725 John J. Kingman Road, Suite 0944 Fort Belvoir, VA 22060-6128 USA Phone: 703-767-9194 Dear Ms. Akers: I request the following records under the provisions of the Freedom of Information Act: A computer generated technical report bibliography [for all computerized index years] of reports on the subject[s]/keyword[s] of: ________________ OR _________________ OR ________________ OR _______________ OR _________________ OR _______________ This is a request for DTIC records, please don't forward my request to NTIS. Please include both classified and unclassified records in your search. If any of the records are classified, please review them for release, or the release of nonsensitive portions. I am an individual, noncommercial requester and this request is not being made for commercial purposes. [OR YOU MIGHT INSTEAD INDICATE IF YOU ARE A COMMERCIAL REQUESTER, OR AN EDUCATIONAL OR SCIENTIFIC INSTITUTION, OR A REPRESENTATIVE OF THE MEDIA] I also agree to pay up to $35 for reasonable fees associated with this request. Sincerely, ______________ From BIOSCI-REQUEST Thu Nov 7 20:42:59 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id UAA28792; Thu, 7 Nov 1996 20:42:59 -0800 Received: from cs.sandia.gov (cs.sandia.gov [132.175.13.2]) by net.bio.net (8.6.12/8.6.6) with SMTP id UAA28787; Thu, 7 Nov 1996 20:42:42 -0800 Received: from frodo2.cs.sandia.gov by cs.sandia.gov with smtp (Smail3.1.28.1 #5) id m0vLe07-000XcRC; Thu, 7 Nov 96 16:36 MST Received: by frodo2.cs.sandia.gov (SMI-8.6/SMI-SVR4) id QAA14988; Thu, 7 Nov 1996 16:33:17 -0700 Date: Thu, 7 Nov 1996 16:33:17 -0700 From: scistra@cs.sandia.gov (Sorin C. Istrail) Message-Id: <199611072333.QAA14988@frodo2.cs.sandia.gov> To: rna@net.bio.net Subject: RECOMB97: Hotel Reservation and Registration Information Please call the ElDorado hotel and make your reservation AS SOON AS POSSIBLE. This is a four-star and four-diamond hotel and we have a limited number of rooms avaiblable. ElDorado will direct the overflow to another hotel. FIRST ANNUAL INTERNATIONAL CONFERENCE ON COMPUTATIONAL MOLECULAR BIOLOGY RECOMB 97 http://www.cs.sandia.gov/recomb97 January 20-23, 1997 Eldorado Hotel Santa Fe, New Mexico Sponsored by SIGACT with support from SLOAN Foundation US Department of Energy ELDORADO HOTEL RESERVATIONS INFORMATION **** A four-star, four diamond-hotel **** Phones: 1-800 955-4455 505-988-4455 Fax: 505-995-4544 (reservations only) Webpage: http://www.eldoradohotel.com Email: rez@eldoradohotel.com Room Rates: Single/double $85 Sunday(1/19), Monday(1/20), Tuesday(1/21), Wednesday(1/22), Thursday(1/23), Friday(1/24) Saturday (1/25) Single/Double $135 Saturday(1/18) (For comparison, if reservations are not made at the RECOMB97 group rate, the average single/double rate would be $144 for these types of rooms.) RECOMB97 REGISTRATION INFORMATION A registration form can be obtained from the conference web page http://www.cs.sandia.gov/recomb97 Advanced Registration ACM Member Non-ACM Member $285 $360 Late/On-site Registration (After December 12) ACM Member Non-ACM Member $360 $435 Full time student Advanced Registration $160 Late/On-site Registration (After Decemebr 12) $210 Registration fee includes: one copy of the proceedings, four lunches, eight coffee breaks, and a banquet ticket. Student registration fee includes all of the above except the banquet ticket. For more information check out the conference web page http://www.cs.sandia.gov/recomb97 or contact Sorin Istrail RECOMB97 Conference Chair Sandia National Laboratories Massively Parallel Computing Research Laboratory MS 1110 Albuquerque, NM 87185-1110 Phone: (505) 845-7612 Fax : (505) 845-7442 Secretary: (505) 845-7432 Email: scistra@cs.sandia.gov www : http://www.cs.sandia.gov/~scistra From BIOSCI-REQUEST Sat Nov 9 02:00:25 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA03436; Sat, 9 Nov 1996 02:00:25 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA03410; Sat, 9 Nov 1996 02:00:11 -0800 Date: Sat, 9 Nov 1996 02:00:11 -0800 From: BIOSCI Administrator Message-Id: <199611091000.CAA03410@net.bio.net> To: rna@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Tue Nov 12 18:38:26 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id SAA10033; Tue, 12 Nov 1996 18:38:26 -0800 Received: from biocserver.BIOC.CWRU.Edu (root@biocserver.BIOC.CWRU.Edu [129.22.152.13]) by net.bio.net (8.6.12/8.6.6) with ESMTP id SAA10018; Tue, 12 Nov 1996 18:38:09 -0800 Received: from [129.22.152.23] (bioc38935.BIOC.CWRU.Edu [129.22.152.23]) by biocserver.BIOC.CWRU.Edu with SMTP (8.7.3+aaa/CWRU-2.1-Linux(BIOC)) id CAA30277; Wed, 13 Nov 1996 02:39:19 GMT (from mcpheete@biocserver.bioc.cwru.edu for ) Date: Wed, 13 Nov 1996 02:39:19 GMT X-Authentication-Warning: biocserver.BIOC.CWRU.Edu: Host bioc38935.BIOC.CWRU.Edu [129.22.152.23] claimed to be [129.22.152.23] Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: rna@net.bio.net From: mcpheete@biocserver.bioc.cwru.edu (David S. McPheeters) Subject: Postdoctoral position November 12, 1996 *********** POSTDOCTORAL RESEARCH OPPORTUNITY *********** A postdoctoral research position is available immediately to study the roles of the yeast small nuclear RNAs and their associated proteins in nuclear pre-mRNA splicing. We are currently taking a combined biochemical and genetic approach to study factors involved in i) the interaction of the yeast U2 small nuclear RNA and the pre-mRNA branch site sequence and ii) the structural transitions in the spliceosome involving the U2 small nuclear RNA. Experience with molecular biology techniques is required and persons with experience in yeast genetics are preferred. Salary is competitive and dependent upon qualifications. English proficiency is also required, as some supervision of graduate students is expected. Please send a curriculum vitae, a brief summary of research experience, and the names of three references to: Dr. David S. McPheeters Department of Biochemistry School of Medicine W432 Case Western Reserve University Cleveland, OH 44106-4935 This research program is part of Case Western Reserve University's Center for RNA Molecular Biology. For more information on the Center for RNA Molecular Biology at CWRU, visit our web site at: http://biocserver.cwru.edu/RNA ------------------------------------------------------------- David S. McPheeters Assistant Professor Department of Biochemistry - School of Medicine W432 Case Western Reserve University Cleveland, OH 44106-4935 mcpheete@biocserver.bioc.cwru.edu (216) 368-8816 FAX (216) 368-4544 ------------------------------------------------------------- From BIOSCI-REQUEST Tue Nov 19 18:04:26 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id SAA24850; Tue, 19 Nov 1996 18:04:26 -0800 Received: from humvee.doit.wisc.edu (humvee.doit.wisc.edu [144.92.9.20]) by net.bio.net (8.6.12/8.6.6) with ESMTP id SAA24837; Tue, 19 Nov 1996 18:04:09 -0800 Received: from audumla.students.wisc.edu by humvee.doit.wisc.edu; id UAA22656; 8.7.5/50; Tue, 19 Nov 1996 20:04:45 -0600 Received: from CISPPP by audumla.students.wisc.edu; id UAA35914; 8.6.9W/42; Tue, 19 Nov 1996 20:04:41 -0600 Message-Id: <199611200204.UAA35914@audumla.students.wisc.edu> Date: Tue, 19 Nov 96 20:06:34 -0800 From: "Anne-Marie K. Walker" Organization: UW-Madison X-Mailer: Mozilla 1.22 (Windows; I; 16bit) MIME-Version: 1.0 To: rna@net.bio.net Subject: LOOK HERE FOR EASY $$$$$ Content-Transfer-Encoding: 7bit Content-Type: text/plain 1(I>(J$BAw(J From BIOSCI-REQUEST Thu Nov 21 02:42:27 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA05172; Thu, 21 Nov 1996 02:42:27 -0800 Received: from haymarket.ed.ac.uk (haymarket.ed.ac.uk [129.215.128.53]) by net.bio.net (8.6.12/8.6.6) with ESMTP id CAA05167; Thu, 21 Nov 1996 02:42:06 -0800 Received: from srv0.bio.ed.ac.uk (srv0.bio.ed.ac.uk [129.215.136.240]) by haymarket.ed.ac.uk (8.6.13/8.6.12) with ESMTP id KAA03537 for ; Thu, 21 Nov 1996 10:42:42 GMT Received: from BIO-SRV0/SpoolDir by srv0.bio.ed.ac.uk (Mercury 1.21); 21 Nov 96 10:42:43 +0000 Received: from SpoolDir by BIO-SRV0 (Mercury 1.21); 21 Nov 96 10:42:14 +0000 From: "Jean Beggs" Organization: Edinburgh University To: RNA@net.bio.net Date: Thu, 21 Nov 1996 10:42:11 +0000 Subject: postdoc position Priority: normal X-mailer: Pegasus Mail for Windows (v2.01) Message-ID: POSTDOCTORAL POSITION INSTITUTE OF CELL AND MOLECULAR BIOLOGY UNIVERSITY OF EDINBURGH, UK A position is available for a postdoctoral molecular biologist to study protein splicing factors in the yeast Saccharomyces cerevisiae in the laboratory of Jean Beggs. Experience in yeast genetics and molecular biology would be deal for this post. Expertise in the analysis of nucleic acid-protein interactions would also be appropriate. This laboratory specialises in the analysis of nuclear pre-mRNA splicing, using a combination of genetic and biochemical approaches to characterise protein splicing factors and RNA-protein interactions (e.g. Cooper et al., 1995, EMBO J. 14:2066-2075; Teigelkamp et al., 1995, EMBO J.14:2602-2612). Accommodation is in new laboratories built and equipped to a very high standard. The post will be funded by the Wellcome Trust for 23 months in the first instance, with salary in the range stlg14,319 - stlg21,519 p.a. (under review; starting salary no higher than stlg18,985). Applications, including a full CV and details (include e-mail addresses) of two referees, to: Professor Jean D. Beggs Institute of Cell and Molecular Biology University of Edinburgh King's Buildings Mayfield Road Edinburgh EH9 3JR UK Tel: 44 131 650 5351 Fax: 44 131 650 8650 From BIOSCI-REQUEST Thu Nov 21 13:22:58 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA06288; Thu, 21 Nov 1996 13:22:58 -0800 Received: from acc (acc.haverford.edu [165.82.1.30]) by net.bio.net (8.6.12/8.6.6) with SMTP id NAA06224; Thu, 21 Nov 1996 13:22:40 -0800 Received: from [165.82.4.208] (s208.hilles_academic.haverford.edu) by acc (4.1/SMI-4.1) id AA08213; Thu, 21 Nov 96 16:21:12 EST Date: Thu, 21 Nov 96 16:21:11 EST Message-Id: <9611212121.AA08213@acc> To: rna@net.bio.net From: msanter@haverford.edu (Mel Santer) Subject: SD sequences in mRNAs in E. coli Is there an estimate of how many mRNAs in E. coli do not have Shine-Dalgarno sequences? From BIOSCI-REQUEST Fri Nov 22 01:04:00 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id BAA14854; Fri, 22 Nov 1996 01:04:00 -0800 Received: from gatekeeper.oss.akzonobel.nl (gatekeeper.oss.akzonobel.nl [192.87.3.2]) by net.bio.net (8.6.12/8.6.6) with ESMTP id BAA14850; Fri, 22 Nov 1996 01:03:42 -0800 Received: (from mail@localhost) by gatekeeper.oss.akzonobel.nl (8.7.5/8.7.3) id KAA18424 for ; Fri, 22 Nov 1996 10:06:19 +0100 (MET) Received: from apou02.akzonobel.nl(145.49.90.250) by gatekeeper.oss.akzonobel.nl via smap (V2.0alpha) id xma018836; Fri, 22 Nov 96 10:06:09 +0100 Received: by apou02.akzonobel.nl id KAA06415; Fri, 22 Nov 1996 10:04:03 GMT Date: Fri, 22 Nov 1996 10:04:03 GMT Received: from umc by apou02.akzonobel.nl via MR/VESTA with conversational-MRIF; Fri, 22 Nov 96 10:04:00 +0000 Posted: Fri, 22 Nov 96 10:01:17 +0000 Sender: bvgemen@am.otbc01.umc.akzonobel.nl From: "Bob van Gemen" Message-ID: <0617011022111996/A44107/OTBV05> App-Message-ID: <0617011022111996/A44107/OTBV05/11ABB2810F00> To: "RNA@NET.BIO.NET" Subject: RNase H recognition sites Sensitivity: Company-Confidential Dear RNA fans, One of the enzymatic activities in the NASBA nucleic amplification reaction is RNase H. The activity is associated with both the AMV-RT and E.coli RNase H. We were wandering if there is anybody who is knows the recognition sites on the RNA for these two enzymes with RNase H activity? In all the literature only very little is described about theses sites, if they exist at all. Any form of help will be appreciated very much. Bob van Gemen Groupleader Molecular Biology Organon Teknika Boseind 15 5281 RM Boxtel The Netherlands Tel: +31-411-654480 Fax: +31-411-654427 E-mail: bvgemen@am.otbc01.umc.akzonobel.nl