From BIOSCI-REQUEST Sun Feb 9 02:00:10 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA08923; Sun, 9 Feb 1997 02:00:10 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA08907; Sun, 9 Feb 1997 02:00:06 -0800 Date: Sun, 9 Feb 1997 02:00:06 -0800 From: BIOSCI Administrator Message-Id: <199702091000.CAA08907@net.bio.net> To: rna@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Tue Feb 11 21:31:40 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id VAA23726; Tue, 11 Feb 1997 21:31:40 -0800 Received: from freelunch.freenet.kiev.ua (root@freelunch.freenet.kiev.ua [194.44.28.250]) by net.bio.net (8.6.12/8.6.6) with ESMTP id VAA23721; Tue, 11 Feb 1997 21:31:33 -0800 Received: from wlab.UUCP (uuwlab@localhost) by freelunch.freenet.kiev.ua (8.8.5/8.8.5) with UUCP id HAA28359 for rna@net.bio.net; Wed, 12 Feb 1997 07:21:32 +0200 (EET) X-Authentication-Warning: freelunch.freenet.kiev.ua: uuwlab set sender to wlab!wlab.freenet.kiev.ua!ivanov using -f Received: by wlab.freenet.kiev.ua (UUPC/@ v5.09gamma, 14Mar93); Tue, 11 Feb 1997 08:33:46 +0200 To: rna@net.bio.net Message-Id: Organization: World Laboratory Ukrainian Branch From: Vladimir Ivanov Date: Tue, 11 Feb 97 08:33:45 +0200 X-Mailer: BML [MS/DOS Beauty Mail v.1.36] Subject: Seeking_INTAS_partners Lines: 64 Dear colleagues , I and my colleagues are looking for the partners of the research projects application and implementation by by the funding of INTAS .Last call of INTAS has dead line at April 2,1997. INTAS, the International Association for the promotion of co-operation with scientists from the New Independent States of the former Soviet Union was established as an European initiative to promote, by an international effort, scientific research activities in the New Independent States and scientific co-operation between scientists in these countries and the international scientific community. The INTAS members are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Israel, Italy, Luxembourg, The Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, the United Kingdom and the European Community. We should like to co-operate with the partners aiming to develop and implement the mentioned below research projects. Field 4.Life Science Subfield 100 General Biology FLOW CYTOFLOUROMETRY ANALYSIS OF TROPHICAL HETEROGENEITY OF BACTERIAL AND YEAST POPULATION Field 4.Life Science Subfield 100 General Biology CONSTRUCTION OF BACTERIAL PHYLOGENETIC TREES BASED ON 16S rRNA SEQUENCE DATA AND G+C CONTENT OF DNA (different phylogenetic groups of bacteria) Field.4 Life Sciences; Subfield 300 Physiology and fundamental Medical Science CONTRACEPTIVE EFFECT OF SOME REPRESENTATIVES FROM VAGINAL MICROFLORA Field 5.Earth Sciences, Environment, Energy Subfield 200 Geochemistry INTERACTION OF NITRIFICATION AND FERROUS OXIDATION IN SOIL AND AQUATIC ECOSYSTEMS Looking forward for mutual research co-operation. Yours sincerely, Vladimir Ivanov ********************************************************** Prof.Vladimir Ivanov Faculty of Biology, Ukrainian National University , 60 Vladimirskaya Str.,Kiev 252017,Ukraine Tel:380-44-266-6206; Tel/Fax :380-44-244-4403 ;380-44-216-7012 E-mail: ivanov@wlab.freenet.kiev.ua ********************************************************** P.S.INTAS information package can be obtained on written request (post,fax or e-mail) INTAS, 58 Avenue des Arts, box 8, B-1000 Brussels, Belgium Fax: +32-2-549 01 56 E-mail: call96@intas.be The information package is also available on the following INTERNET sites: CORDIS: http://www.cordis.lu/intas/home.html INTAS: http://www.ib.be/intas From BIOSCI-REQUEST Wed Feb 19 07:54:19 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id HAA13671; Wed, 19 Feb 1997 07:54:19 -0800 Received: from dino.nci.nih.gov (dino.nci.nih.gov [156.40.115.200]) by net.bio.net (8.6.12/8.6.6) with ESMTP id HAA13660; Wed, 19 Feb 1997 07:54:16 -0800 Received: from [156.40.227.193] by dino.nci.nih.gov (Netscape Mail Server v2.01) with ESMTP id AAA16390 for ; Wed, 19 Feb 1997 10:54:05 -0400 X-Sender: harfordj@pop.nci.nih.gov Message-Id: Mime-Version: 1.0 Content-Type: text/enriched; charset="us-ascii" Date: Wed, 19 Feb 1997 10:55:57 -0500 To: rna@net.bio.net From: Joe Harford Subject: San Francisco Symp. 97: Transaltion & Stability of mRNA CourierSan Francisco Symposium 97: Translation and Stability of mRNA will be held Oct. 12-14, 1997 at the San Francisco Airport Hilton Hotel. The meeting is being organized by Joe Harford, Michael Katze, and James Larrick. A similar, highly successful meeting was held in 1995. The speakers for SF97 will include: Joel Belasco, Tom Dever, Tom Donahue, Gideon Dreyfus, Ellie Ehrenfeld, Stanley Fields, Elizabeth Goodwin, Mathias Hentze, Allan Jacobson, Michael Katze, Jack Keene, Ruth Lehmann, Lynne Maquat, William Merrick, Joel Richter, Jeff Ross, Alan Sachs, Daniel Schoenberg, Robert Singer, and Nahum Sonenberg. The Keynote Lecture will be given by Paul Schimmel. Opportunity will be provided for poster presentations by other participants. For additional information re. registration, email paimm@netgat.net or fax 415-694-7717. Since space is limited, registration will be on a first-come-first-serve basis. Joe Harford, Ph.D. Assoc. Director for Special Projects National Cancer Institute Bldg. 31, Rm. 11A48 Bethesda, MD 20892 Phone 301-496-5534 Fax 301-496-2471 From BIOSCI-REQUEST Wed Feb 19 09:29:09 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA27134; Wed, 19 Feb 1997 09:29:09 -0800 Received: from dino.nci.nih.gov (dino.nci.nih.gov [156.40.115.200]) by net.bio.net (8.6.12/8.6.6) with ESMTP id JAA27130; Wed, 19 Feb 1997 09:29:07 -0800 Received: from [156.40.227.193] by dino.nci.nih.gov (Netscape Mail Server v2.01) with ESMTP id AAA17494 for ; Wed, 19 Feb 1997 12:28:56 -0400 X-Sender: harfordj@pop.nci.nih.gov Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 19 Feb 1997 12:30:53 -0500 To: rna@net.bio.net From: Joe Harford Subject: email correction The correct email address for inquiries re. San Francisco Symposium 97: Tranlation & Stability of mRNA is paimm@netgate.net. Joe Harford, Ph.D. Assoc. Director for Special Projects National Cancer Institute Bldg. 31, Rm. 11A48 Bethesda, MD 20892 Phone 301-496-5534 Fax 301-496-2471 From BIOSCI-REQUEST Fri Feb 28 16:16:38 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id QAA06701; Fri, 28 Feb 1997 16:16:38 -0800 Received: from sun1.tcd.ie (sun1.tcd.ie [134.226.1.29]) by net.bio.net (8.6.12/8.6.6) with ESMTP id QAA06684; Fri, 28 Feb 1997 16:16:34 -0800 Received: from 134.226.67.33 (sci67033.sci.tcd.ie [134.226.67.33]) by sun1.tcd.ie (8.8.5/8.7.3) with SMTP id AAA23880 for ; Sat, 1 Mar 1997 00:17:29 GMT Message-ID: <331767C5.2CB4@tcd.ie> Date: Sat, 01 Mar 1997 00:18:29 +0100 From: Adrian Bracken Reply-To: brackena@tcd.ie Organization: Trinity College, Dublin Ireland X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) MIME-Version: 1.0 To: rna@net.bio.net Subject: POLYACRLAMIDE NORTHERNS FOR LARGE BANDS? X-URL: http://www.bio.net/hypermail/RNA/ Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit IS IT POSSIBLE TO RUN A POLY ACRLAMIDE (3.5%) - UREA INSTEAD OF A FORMALDEHYDE AGAROSE FOR RESOLVING BANDS IN THE RANGE 1000 - 2000 AND THEN DO A ELECTROBLOT NORTHERN, PLEASE TELL ME IT IS...RIGHT NOW A FORMALDEHYDE GEL IS NOT POSSIBLE IN THIS LAB. (AND ANYWAY I AM WELL PRACTISED AT POLYACRLAMIDE RNA GELS AND ELECROBLOTTING NORTHERNS). PLEASE MAIL ME ASAP AT BRACKENA@TCD.IE. THANK YOU, ADRIAN BRACKEN.