From BIOSCI-REQUEST Fri Apr 4 05:03:07 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id FAA07797; Fri, 4 Apr 1997 05:03:07 -0800 Received: from MSSERV.RZ-Berlin.MPG.DE (msserv.RZ-Berlin.MPG.DE [141.14.129.143]) by net.bio.net (8.6.12/8.6.6) with SMTP id FAA07794; Fri, 4 Apr 1997 05:03:05 -0800 Received: from 158.250.34.26.genebee.msu.su (ribosome.genebee.msu.su) by MSSERV.RZ-Berlin.MPG.DE (MX V4.1 VAX) with SMTP; Fri, 04 Apr 1997 15:03:15 +0100 Message-ID: <3344F564.7B9@mpimg-berlin-dahlem.mpg.de> Date: Fri, 04 Apr 1997 16:34:44 +0400 From: Pavel Baranov Reply-To: baranov@mpimg-berlin-dahlem.mpg.de Organization: Moscow State University X-Mailer: Mozilla 3.0 (Win95; I) MIME-Version: 1.0 To: rna@net.bio.net Subject: dye for electrophoresis Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear colleges! I'd appreciate very much if you'll inform me about any dye for gels (like xylene cyanol) that migrates in 4% denaturating gel about 300-1000 bp rna or dna. Thanks. -- Pavel V. Baranov Moscow State University http://www.mpimg-berlin-dahlem.mpg.de/~baranov/ ftp://ribosome.genebee.msu.su tel: (095)-939-54-18 fax: (095)-939-31-81 From BIOSCI-REQUEST Fri Apr 4 05:44:39 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id FAA13572; Fri, 4 Apr 1997 05:44:39 -0800 Received: from MSSERV.RZ-Berlin.MPG.DE (msserv.RZ-Berlin.MPG.DE [141.14.129.143]) by net.bio.net (8.6.12/8.6.6) with SMTP id FAA13562; Fri, 4 Apr 1997 05:44:37 -0800 Received: from 158.250.34.26.genebee.msu.su (ribosome.genebee.msu.su) by MSSERV.RZ-Berlin.MPG.DE (MX V4.1 VAX) with SMTP; Fri, 04 Apr 1997 15:44:58 +0100 Message-ID: <3344FF2F.2DAC@mpimg-berlin-dahlem.mpg.de> Date: Fri, 04 Apr 1997 17:16:31 +0400 From: Pavel Baranov Reply-To: baranov@mpimg-berlin-dahlem.mpg.de Organization: Moscow State University X-Mailer: Mozilla 3.0 (Win95; I) MIME-Version: 1.0 To: rna@net.bio.net Subject: dye for electrophoresis Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear colleges! I'd appreciate very much if you'll inform me about any dye for gels (like xylene cyanol) that migrates in 4% denaturating gel about 300-1000 bp rna or dna. Thanks. -- Pavel V. Baranov Moscow State University http://www.mpimg-berlin-dahlem.mpg.de/~baranov/ ftp://ribosome.genebee.msu.su tel: (095)-939-54-18 fax: (095)-939-31-81 From BIOSCI-REQUEST Wed Apr 9 02:00:14 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA28202; Wed, 9 Apr 1997 02:00:14 -0700 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA28176; Wed, 9 Apr 1997 02:00:06 -0700 Date: Wed, 9 Apr 1997 02:00:06 -0700 From: BIOSCI Administrator Message-Id: <199704090900.CAA28176@net.bio.net> To: rna@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Wed Apr 9 17:58:40 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id RAA01562; Wed, 9 Apr 1997 17:58:40 -0700 Received: from pobox1.oit.umass.edu (mailhub.oit.umass.edu [128.119.166.151]) by net.bio.net (8.6.12/8.6.6) with ESMTP id RAA01531 Received: from wash2214.umass.edu (dh10-112.res.umass.edu) by pobox1.oit.umass.edu (PMDF V5.1-5 #20973) with SMTP id <0E8EDEQPB002CW@pobox1.oit.umass.edu>; Wed, 9 Apr 1997 20:59:15 -0400 (EDT) Date: Wed, 09 Apr 1997 20:57:27 -0400 From: Brian Scott Subject: Free molecular visualization workshops for profs To: protein-analysis@net.bio.net, rapd@net.bio.net, recom@net.bio.net, rna@net.bio.net, staden@net.bio.net, str-nmr@net.bio.net, x-plor@net.bio.net Reply-to: bscott@biochem.umass.edu Message-id: <334C3AF7.6EA7@biochem.umass.edu> Organization: University of Massachusetts MIME-version: 1.0 X-Mailer: Mozilla 3.01Gold [de] (Win95; I) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit Free Workshops on Molecular Visualization for Undergraduate Bioscience Teaching Amherst MA -- Summer, 1997-8 Understanding the three-dimensional structures of proteins, DNA, RNA, and their interactions is difficult from flat pictures, yet grasping structure is important to understanding function. Free software is now available which displays attention-grabbing 3D animated images of biological molecules in depth-cued spacefilling color. RasMol (http://www.umass.edu/microbio/rasmol), which works on Windows or Macs, encourages self-directed exploration. Chime (http://www.umass.edu/microbio/chime) allows annotated preset views of molecules to be delivered as web tutorials. Free, hands-on workshops will be held this summer at the University of Massachusetts in Amherst to prepare college faculty with no prior experience to use molecular visualization in their classes. Participants will travel to the Amherst campus on three separate days (two this summer, one the following summer), and are responsible for their own travel expenses (and therefore are expected primarily from the Northeastern USA). Overnight accomodations will be provided free to those needing them (based on distance traveled). Participants will be expected to incorporate molecular visualization into their teaching, and to mentor two faculty colleagues at their home institution. The workshops will be led by Eric Martz, a Professor in the Department of Microbiology. Martz has innovated molecular structure tutorials which are in use throughout the USA and in dozens of other countries. The web site he created (see above) was visited by 40,000 people in 1996. 1997 dates are June 17 and 30 (workshop A), or June 19 and July 2 (workshop B). Workshops A and B also meet for one day in late June 1998. To obtain more detailed information and a REGISTRATION FORM, visit http://www.umass.edu/microbio/rasmol/workshop.htm or FAX a request to 413-545-1578, or email a request to emartz@microbio.umass.edu. Supported by the National Science Foundation (Undergraduate Faculty Enhancement, Division of Undergraduate Education) and the University of Massachusetts. /* - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor (Immunology), Dept Microbiology, Univ Massachusetts, Amherst MA US 01003-5720 413-545-2325 FAX:545-1578. RasMol Home Page, http://www.umass.edu/microbio/rasmol Other web projects listed at http://www.umass.edu/microbio/rasmol/em-web.htm - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/ From BIOSCI-REQUEST Tue Apr 22 07:35:02 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id HAA10132; Tue, 22 Apr 1997 07:35:02 -0700 Received: from mail.bus.wisc.edu (mail.bus.wisc.edu [144.92.9.29]) by net.bio.net (8.6.12/8.6.6) with ESMTP id HAA10124; Tue, 22 Apr 1997 07:35:00 -0700 Received: from 144.92.64.222 by mail.bus.wisc.edu; id JAA26420; 8.7.5/50; Tue, 22 Apr 1997 09:27:39 -0500 Message-ID: <335C40AC.1BFB@dfrc.wisc.edu> Date: Tue, 22 Apr 1997 08:38:08 +0400 From: Chris Odt Reply-To: chriso@dfrc.wisc.edu Organization: USDA X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) MIME-Version: 1.0 To: RNA@net.bio.net Subject: subscribe rna Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit subscribe rna From BIOSCI-REQUEST Wed Apr 23 20:31:28 1997 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id UAA13360; Wed, 23 Apr 1997 20:31:28 -0700 Received: from UConnVM.UConn.Edu (uconnvm.uconn.edu [137.99.26.3]) by net.bio.net (8.6.12/8.6.6) with SMTP id UAA13357; Wed, 23 Apr 1997 20:31:26 -0700 Received: from UCONNVM.UCONN.EDU by UConnVM.UConn.Edu (IBM VM SMTP V2R2) with BSMTP id 9463; Wed, 23 Apr 97 23:32:21 EDT Received: from UConnVM.UConn.Edu (NJE origin RHODES@UCONNVM) by UCONNVM.UCONN.EDU (LMail V1.2c/1.8c) with BSMTP id 4357; Wed, 23 Apr 1997 23:32:21 -0400 Date: Wed, 23 Apr 97 23:30:45 EDT From: RHODES@uconnvm.uconn.edu Subject: RE: RNase H (fwd) To: rna@NET.BIO.NET X-Mailer: MailBook 96.02.327 Message-Id: <970423.233221.EDT.RHODES@UConnVM.UConn.Edu> MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Content-Transfer-Encoding: 7BIT Sorry if this is redundant, but the original post to HIV-biol brought no replies...anyone here with information to share??? >One of those things that apparently slips by when an area of >research (albeit huge) is somewhat afield of one's own area... >That's the only way I can rationalize the newfound (to ME) >concept of RNase H being responsible for removing the rna >after the reverse transcriptase has had its fun, as has now >been pointed out to me in a couple of very general references. >The problem is that the references are _very_ general, and the >putative action of RNase H in this capacity was referred to >only in figures, as an aside, and not discussed explicitly. >It makes sense that such a ubiquitous enzyme should interact >with an RNA/DNA hybrid, but I'd like to know more..... > >Is RNase H actually involved in the infection process or is >this another function of the multi-talented RT? Can anyone >refer me to a good discussion of this topic?? Please respond >directly to me, as I am not a subscriber to HIV-biol. (or RNA) > >thanks 10^6! | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-4998 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O |