From BIOSCI-REQUEST Fri Jan 2 20:38:11 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id UAA04325; Fri, 2 Jan 1998 20:38:11 -0800 Received: from dns01.ops.usa.net (dns01.ops.usa.net [204.68.24.137]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id UAA04322; Fri, 2 Jan 1998 20:38:09 -0800 Received: (qmail 14116 invoked by alias); 3 Jan 1998 04:37:23 -0000 Received: (qmail 13996 invoked from network); 3 Jan 1998 04:37:14 -0000 Received: from unknown (HELO usa.net) (202.103.3.228) by dns01.ops.usa.net with SMTP; 3 Jan 1998 04:37:14 -0000 Message-ID: <34ADC001.5A347B2@usa.net> Date: Sat, 03 Jan 1998 12:35:13 +0800 From: zhaojun1 Reply-To: zhaojun1@usa.net X-Mailer: Mozilla 4.04 [en] (Win95; I) MIME-Version: 1.0 To: rna@net.bio.net Subject: help me design ribozyme! Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear Sir, I'd like to design and synthesize ribozyme, unfortunately, I do not have appropriate PC program, I want to know who or which company can design and synthesize ribozyme? My e mail is: zhaojun1@usa.net Thanks for your kind help! Zhao Jun, MD From BIOSCI-REQUEST Mon Jan 5 16:23:56 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id QAA13473; Mon, 5 Jan 1998 16:23:56 -0800 Received: from genome.biotech.washington.edu (genome.biotech.washington.edu [128.95.231.12]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id QAA13470; Mon, 5 Jan 1998 16:23:55 -0800 Received: (from eugene@localhost) by genome.biotech.washington.edu (8.8.5/8.8.5) id QAA23650 for rna@net.bio.net; Mon, 5 Jan 1998 16:23:11 -0800 Date: Mon, 5 Jan 1998 16:23:11 -0800 From: Eugene Kolker Message-Id: <199801060023.QAA23650@genome.biotech.washington.edu> To: rna@net.bio.net Subject: Recomb98: Registration CALL FOR RECOMB 98 Registration Registration is now starting for RECOMB 98. Please visit our website for registration instructions: http://www.mssm.edu/biomath/recomb98.html If you do NOT want to be in our mailing list, please reply with Subject line "Recomb98: remove". From BIOSCI-REQUEST Fri Jan 9 02:00:13 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA21205; Fri, 9 Jan 1998 02:00:13 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA21189; Fri, 9 Jan 1998 02:00:08 -0800 Date: Fri, 9 Jan 1998 02:00:08 -0800 From: BIOSCI Administrator Message-Id: <199801091000.CAA21189@net.bio.net> To: rna@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From BIOSCI-REQUEST Fri Jan 9 10:46:40 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id KAA21025; Fri, 9 Jan 1998 10:46:40 -0800 Received: from snipe.biotech.ufl.org (snipe.biotech.ufl.org [128.227.25.4]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id KAA21015; Fri, 9 Jan 1998 10:46:36 -0800 Received: from biotech.ufl.org by snipe.biotech.ufl.org (8.7/4.09) id NAA24114; Fri, 9 Jan 1998 13:45:50 -0500 (EST) Message-ID: <34B66FFD.16625DA3@biotech.ufl.org> Date: Fri, 09 Jan 1998 13:44:13 -0500 From: Martin Munzer Reply-To: mmunzer@biotech.ufl.org Organization: CyGene, Inc. X-Mailer: Mozilla 4.02 [en]C-DIAL (WinNT; U) MIME-Version: 1.0 To: rna@net.bio.net Subject: New DNA technology Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit New DNA technology The following text is a press release sent out Wednesday through PRNewswire. Contrary to the hype and hysteria over human cloning, these technologies will prove of great benefit to mankind and be available soon. If this does not interest you, please forward it to someone who might be interested. Thanks, Martin Munzer PRESS RELEASE Florida Biotech Companies Fill DNA Technology Gap with New Diagnostic Technologies Alachua, Florida, Jan. 7, /PRNewswire/ -- AGENDA, Inc. and CyGene, Inc., two Florida based biotech companies, have signed a co-licensing agreement to market their next-generation DNA diagnostic technologies. Under the terms of the agreement, AGENDA will market CyGene's patent pending TPA (TM) technology exclusively, and in conjunction with its own RFTA (TM) technology. 1997 was an exceptional year for DNA discoveries. Important genetic sequences, some responsible for diseases such as Alzheimer's, Rheumatoid Arthritis, Cancers, Heart Disease, etc. were recognized. These were added to the huge arsenal of already recognized genetic sequences like HIV, Hepatitis C and numerous contagious or hereditary diseases. One critical tool missing, which would add to the value of these discoveries, is a technology that will quickly, reliably and economically recognize these specific genes when only a few copies are present. AGENDA's RFTA (TM) (patent pending) technology complements TPA (TM) in that both will detect any known genetic sequence while using different methods. These technologies make early reliable diagnosis of HIV, Hepatitis C, cancer, parasitic, viral, bacterial and hereditary diseases a reality. Both processes can be automated, to provide fast and unprecedented first and second opinions regarding the presence or absence of the targeted genes. The ability of these technologies to handle large quantities of DNA or RNA set them apart from other techniques, which are prone to false results. For the first time, very early disease recognition (before the onset of clinical symptoms) is made possible by these next-generation DNA diagnostic technologies. This will open the door to entirely new approaches to disease prevention, management and cure. Early stage diagnosis will make cure scenarios a reality, for many terminal diseases. First generation DNA technology can only reliably detect HIV, 6-7 months post infection. TPA/RFTA (TM) will be able to detect HIV accurately shortly after the time of infection. Similar to computer operating systems, RFTA (TM) and TPA (TM) represent a technology base from which applications can be developed for agricultural, veterinary, health sciences, and human diagnostics. Based on proven molecular biology principles, both technologies can be configured to complete automation and may be performed in tandem using a single sample. AGENDA is actively seeking licensees and companies involved in the design and production of biotech automation equipment. This is the first public announcement of the existence of TPA (TM) and RFTA (TM). Contact: Martin Munzer, Executive VP, 904-418-1616 Email mmunzer@dna-diagnostics.com http://www.dna-diagnostics.com/ and http://www.cygene.com From BIOSCI-REQUEST Tue Jan 13 08:36:02 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id IAA29640; Tue, 13 Jan 1998 08:36:02 -0800 Received: from postoffice.webcow.com (postoffice.webcow.com [206.251.6.250]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id IAA29637; Tue, 13 Jan 1998 08:36:01 -0800 Received: from cygene-2.biotech.ufl.org ([128.227.25.117]) by postoffice.webcow.com (Post.Office MTA v3.1 release PO203a ID# 0-41990U5000L500S0) with SMTP id AAA10635 for ; Tue, 13 Jan 1998 08:35:12 -0800 Received: by cygene-2.biotech.ufl.org with Microsoft Mail id <01BD2017.120CD750@cygene-2.biotech.ufl.org>; Tue, 13 Jan 1998 11:33:29 -0500 Message-ID: <01BD2017.120CD750@cygene-2.biotech.ufl.org> From: mmunzer@dna-diagnostics.com (dna-diagnostics.com) To: "'rna@net.bio.net'" Subject: Re: New DNA/RNA Technologies Date: Tue, 13 Jan 1998 11:28:54 -0500 MIME-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Thank you all for your overwhelmingly positive response to our = announcement of new DNA Diagnostic technologies. Due to the volume of = inquiries, we have compiled a series of FAQ's, to respond to the = majority of your questions. We have posted them on our CyGene web site = along with several letters of opinion from professionals who have = reviewed the TPA and RFTA patent documents. RFTA opinion letters will be = posted on our AGENDA site shortly. We will respond to all other inquiries as soon as possible. These = technologies also detect RNA sequences. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Martin Munzer=20 AGENDA, Inc. http://www.dna-diagnostics.com a.k.a. Advanced Genetic Diagnostic Associates=20 Confirming "Heirlines" Through State of the Art DNA Analysis ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From BIOSCI-REQUEST Wed Jan 21 08:54:30 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id IAA22569; Wed, 21 Jan 1998 08:54:30 -0800 Received: from agr.agr.kuleuven.ac.be ([134.58.73.1]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id IAA22566; Wed, 21 Jan 1998 08:54:27 -0800 Received: by agr.agr.kuleuven.ac.be with VINES-ISMTP; Wed, 21 Jan 98 18:53:01 +0200 Date: Wed, 21 Jan 98 18:53:00 +0200 Message-ID: X-Priority: 3 (Normal) To: From: "Ilse Wiame" Reply-To: Errors-to: Subject: How to quantitate RT-PCR? X-Incognito-SN: 297 X-Incognito-Version: 4.11.2 MIME-Version: 1.0 Content-type: text/plain; charset=us-ascii Is there a plant gene that is encoded in the nucleus and that is constitutively expressed in plants in all cell types and in every stage of development? I want to use primers designed to that gene to quantitate RT-PCR. Or are there other ways to quantitate RT-PCR in plants? From BIOSCI-REQUEST Thu Jan 29 07:56:26 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id HAA12564; Thu, 29 Jan 1998 07:56:26 -0800 Received: from VM1.MCGILL.CA (VM1.McGill.CA [132.206.27.2]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id HAA12415 Date: Thu, 29 Jan 1998 07:55:59 -0800 From: dinkmaster@hotmail.com Message-Id: <199801291555.HAA12415@net.bio.net> Received: from hotmail.com by VM1.MCGILL.CA (IBM VM SMTP V2R3) with TCP; Thu, 29 Jan 98 10:54:58 EST To: dinkmaster@hotmail.com Subject: Smith Family Reunion Hi there Smiths! It's that time again! It seems like every time we turn around, it's reunion time again. Has it really been five years? Meg and I are in charge of it this go round, so we'd like to start by filling you in with what is going on in these Smith's neck of the woods. We are living in Guam, as most of you know. Meg just birthed a healthy baby in December, named Pat. As most of you know from our Christmas letter, As you may recall, Pat is a eunich. Thank you all for the kind words of inspitation. We love Pat dearly. Speaking of love, we love it here, but it tends to get a bit wet in monsoon season. Our youngest son, Tom Jr. got caught in a gullywasher, but our trusty pooch Lucky pulled him out before the waterfall. (Yay Lucky! Way to go!) Anyway, the reunion is set for April 17th, 18th, and 19th at Yellowstone. We have made special arrangements, and will have a whole campground to ourselves. Take the main road in, and hit side trail #34 up to campground 34c. We will be having a potluck supper Friday night, so everybody bring their best dish and their appatites! (Linda, that means your ambrosia salad!! YUM!) On a sadder note, Uncle Robert fell and broke his hip, so please call him and wish him a speedy recovery so he might be able to make the reunion. Be sure to RSVP to me ASAP. I have to have a complete headcount by Feburary 1st. We can't wait to see you there. Afterall, blood is thicker that water. Daniel "Dinkmaster" Smith P.S. Aunt Becky was cleard of all charges, so it is safe to bring the kids! Yay Aunt Becky!!!!!