From BIOSCI-REQUEST Mon May 4 16:49:35 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id QAA28420; Mon, 4 May 1998 16:49:35 -0700 Received: from 32.100.128.122 (slip-32-100-128-122.nc.us.ibm.net [32.100.128.122]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id QAA28035 Date: Mon, 4 May 1998 16:48:45 -0700 Message-Id: <199805042348.QAA28035@net.bio.net> From: algree@mailexcite.com To: Medical, Professionals Subject: Free Allergy Screening! X-Reply-To: www.freeyellow.com/members3/alrgee/ Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit /////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// You can now add allergy screening and custom immunotherapy to your practice- and there are no upfront costs. Benefits include continuity of care for the patient, excellent desensitization programming, and significantly increased revenues for your practice. Complete program includes training, laboratory based allergy screening, and complete immunotherapy program in injection ready format. See how "Allergy Treatment is Made Easy" through the BioSafe, "Is Life Making You Sick" allergy treatment program. Register for your free allergy screening kits and complete information package: http://www.freeyellow.com/members3/alrgee/ From BIOSCI-REQUEST Tue May 5 00:14:09 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id AAA19518; Tue, 5 May 1998 00:14:09 -0700 Received: from wap-serv.mti.uni-jena.de (wap-serv.mti.uni-jena.de [141.35.65.141]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id AAA19515; Tue, 5 May 1998 00:14:07 -0700 Received: from [141.35.66.162] ([141.35.66.162]) by wap-serv.mti.uni-jena.de (AIX4.3/UCB 8.7/8.7) with SMTP id JAA14362; Tue, 5 May 1998 09:12:12 +0200 (METDST) X-Authentication-Warning: wap-serv.mti.uni-jena.de: Host [141.35.66.162] didn't use HELO protocol Message-ID: <354F3A4F.3962@mti-n.uni-jena.de> Date: Tue, 05 May 1998 09:11:59 -0700 From: Ulrike Gausmann Reply-To: ugau@mti-n.uni-jena.de Organization: Klinikum der FSU Jena, Anatomie II X-Mailer: Mozilla 3.0Gold (Win16; I) MIME-Version: 1.0 To: rna@net.bio.net CC: ugau@mti-n.uni-jena.de Subject: Help: T7 in vitro transcription - premature termination? Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Hallo, I am performing in vitro transcription using T7 RNA polymerase (run-off transcription, Ribomax-Kit). My controls work fine, no matter if I include CAP analogue or not. Now I subcloned another sequence in frame to my reporter gene and the result is the following: These RNA samples (with and also without CAP analogue) analyzed with gel electrophoresis show degradation (?) or premature termination. Since I had RNase contamination before, I know how degraded RNA looks like - the gel pattern now seems to contain mostly discrete bands. The size of the largest RNA species is exactly what I expect from the fusion gene. I decreased the reaction temperature to 30 degrees which did not result in more complete transcript. Any ideas? Now I am looking for T7 termination signals in my construct which should be special hairpin structures (am I right?). Does anybody know how to detect these sequences (I do not have access to Unix computers)? Thank you very much for any help, Ulrike -- Ulrike Gausmann | D-07743 Jena Institut fuer Anatomie | Germany Anatomie II | Tel +49 (0)3641 938553 Teichgraben 7 | email ugau@mti-n.uni-jena.de From BIOSCI-REQUEST Thu May 7 09:43:59 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id JAA12008; Thu, 7 May 1998 09:43:59 -0700 Received: from fox.tamu.edu (root@unix.tamu.edu [128.194.103.25]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id JAA11991; Thu, 7 May 1998 09:43:48 -0700 Received: from medpath-leibowitz-lab.tamu.edu (medpath-leibowitz-lab.tamu.edu [165.91.32.220]) by fox.tamu.edu (8.8.8/8.8.8) with SMTP id LAA00941 for ; Thu, 7 May 1998 11:41:31 -0500 (CDT) Message-ID: <3552002E.2F1B@medicine.tamu.edu> Date: Thu, 07 May 1998 11:40:46 -0700 From: SANTOSH KUMAR NANDA Reply-To: nanda@medicine.tamu.edu Organization: TAMU X-Mailer: Mozilla 3.0 (Win16; I) MIME-Version: 1.0 To: rna@net.bio.net Subject: Reg: Purification of RNA probe Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Hi, I have to purify invitro transcribed RNA of high specific activity in a smaller volume. Could you suggest me necessary steps to take care or any purification system. Thanks in advance Santosh NAnda Texas A&M University nanda@medicine.tamu.edu From BIOSCI-REQUEST Thu May 7 10:50:37 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id KAA24870; Thu, 7 May 1998 10:50:37 -0700 Received: from 166.55.58.27 (usr29-dialup27.mix2.Atlanta.mci.net [166.55.58.27]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id KAA24427 Date: Thu, 7 May 1998 10:50:05 -0700 Message-Id: <199805071750.KAA24427@net.bio.net> From: Screening@mailexcite.com To: Medical, Professional Subject: Free Allergy Screening! X-Reply-To: www.freeyellow.com/members3/algree/ Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit ///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// You can now add allergy screening and custom immunotherapy to your practice- and there are no upfront costs. Benefits include continuity of care for the patient, excellent desensitization programming, and significantly increased revenues for your practice. Complete program includes training, laboratory based allergy screening, and complete immunotherapy program in injection ready format. See how "Allergy Treatment is Made Easy" through the BioSafe, "Is Life Making You Sick" allergy treatment program. Register for your free allergy screening kits and complete information package: http://www.freeyellow.com/members3/alrgee/ From BIOSCI-REQUEST Thu May 7 21:26:57 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id VAA13210; Thu, 7 May 1998 21:26:57 -0700 Received: from GRACE1.SPANIT.COM (grace1.spanit.com [204.50.114.1]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id VAA12898 Received: from laparkan (port404.spanit.com [204.50.114.68]) by GRACE1.SPANIT.COM (8.8.5/8.7.3) with SMTP id AAA25907; Fri, 8 May 1998 00:24:05 -0400 (EDT) Date: Fri, 8 May 1998 00:24:05 -0400 (EDT) Message-Id: <199805080424.AAA25907@GRACE1.SPANIT.COM> X-Sender: noni@drlarose.com From: "Dr. J. La Rose" To: Your Health! Subject: Important Health Announcement Reply-To: noni@drlarose.com MIME-Version: 1.0 Content-Type: multipart/mixed; boundary=XX8DFF8DBB-00CE8DFFXX Content-Transfer-Encoding: 7bit Noni! Noni! Noni! No! You are not receiving Email in a different language, so don't panic. My name is Dr. La Rose and I am taking the liberty of popping up in your Email to tell you about a simple fruit that is being called the most important natural health discovery for decades. It will only take you a few moments and I urge you to find out how adding a few ounces of juice from a single fruit to your diet, may significantly improve your health and well being. Do visit the web site. http://www.drlarose.com It will be worth your time. I promise!!! Thank you for your interest and best wishes for your good health. Ia-Orana! --XX8DFF8DBB-00CE8DFFXX Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Noni! Noni! Noni!

No! You are not receiving Email in a different language, so don't panic.

My name is Dr. La Rose and I am taking the liberty of popping up
in your Email to tell you about a simple fruit that is being called
the most important natural health discovery for decades.

It will only take you a few moments and I urge you to find out
how adding a few ounces of juice from a single fruit to your diet,
may significantly improve your health and well being.

Do visit the web site.
http://www.drlarose.com

It will be worth your time. I promise!!!

Thank you for your interest and best wishes for your good health.

Ia-Orana! --XX8DFF8DBB-00CE8DFFXX-- From BIOSCI-REQUEST Sat May 9 02:00:13 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA04336; Sat, 9 May 1998 02:00:13 -0700 Received: (from biohelp@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA04308; Sat, 9 May 1998 02:00:09 -0700 Date: Sat, 9 May 1998 02:00:09 -0700 From: BIOSCI Administrator Message-Id: <199805090900.CAA04308@net.bio.net> To: rna@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From BIOSCI-REQUEST Tue May 12 02:31:08 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id CAA09976; Tue, 12 May 1998 02:31:08 -0700 Received: from pop.awi-bremerhaven.de (pop.AWI-Bremerhaven.DE [134.1.2.139]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id CAA09973; Tue, 12 May 1998 02:31:06 -0700 Received: from bio2p12.awi-bremerhaven.de ([134.1.7.66]) by pop.awi-bremerhaven.de (Netscape Mail Server v2.0) with SMTP id AAA30407 for ; Tue, 12 May 1998 11:25:13 +0100 Message-ID: <35581685.45A@awi-bremerhaven.de> Date: Tue, 12 May 1998 11:29:41 +0200 From: "Rene Groben" Reply-To: rgroben@awi-bremerhaven.de Organization: Alfred-Wegener-Institute X-Mailer: Mozilla 3.01 (Win95; I) MIME-Version: 1.0 To: rna@net.bio.net Subject: RNA-Protein binding Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear colleagues, can anyone tell me, which parts/nucleotide positions of 16S, 18S and/ or 28S rRNA binds to the proteins in ribosomes? Thank you, Rene Groben -- Dr. Rene Groben Alfred Wegener Institute for Polar and Marine Research BIO II Am Handelshafen 12 27570 Bremerhaven Germany Tel.: +49 (0)471/4831452 Fax.: +49 (0)471/4831425 e-Mail: rgroben@awi-bremerhaven.de From BIOSCI-REQUEST Tue May 12 07:20:57 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id HAA17365; Tue, 12 May 1998 07:20:57 -0700 Received: from nun.oit.unc.edu (nun.oit.unc.edu [152.2.25.66]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id HAA17362; Tue, 12 May 1998 07:20:55 -0700 Received: from localhost (jbullard@localhost) by nun.oit.unc.edu (8.8.7/8.8.7) with SMTP id KAA19412; Tue, 12 May 1998 10:18:44 -0400 (EDT) Date: Tue, 12 May 1998 10:18:44 -0400 (EDT) From: Jim Bullard To: Rene Groben cc: rna@net.bio.net Subject: Re: RNA-Protein binding In-Reply-To: <35581685.45A@awi-bremerhaven.de> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Rene, There are countless papers out there dealing with positions of the different ribosomal proteins relative to the ribosomal RNA and tRNA and mRNA. I would suggest looking some of these papers up and a good start would be doing a Medline search by author using some of these names: Brimacombe, Noller, Zimmerman, Nierhaus, Dahlberg, Hill, Wintermeyer and others. There is also several models including those by Brimacombe and Frank. You may be opening a can of worms. Good luck. Jim Bullard CB# 3290 Venable Hall University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Lab 919-966-3184 Fax 919-966-3675 e-mail jbullard@nun.oit.unc.edu On Tue, 12 May 1998, Rene Groben wrote: > Dear colleagues, > > can anyone tell me, which parts/nucleotide positions of 16S, 18S and/ > or 28S rRNA binds to the proteins in ribosomes? > > Thank you, > > Rene Groben > > -- > Dr. Rene Groben > Alfred Wegener Institute for Polar and Marine Research > BIO II > Am Handelshafen 12 > 27570 Bremerhaven > Germany > > Tel.: +49 (0)471/4831452 > Fax.: +49 (0)471/4831425 > e-Mail: rgroben@awi-bremerhaven.de > From BIOSCI-REQUEST Mon May 18 12:10:56 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id MAA27886; Mon, 18 May 1998 12:10:56 -0700 Received: from client3.server (209-142-5-162.stk.inreach.net [209.142.5.162]) by net.bio.net (8.6.12-r-beta/8.6.6) with SMTP id MAA27419 From: mary3456@yahoo.com Message-Id: <199805181908.MAA27419@net.bio.net> Date: Mon, 18 May 1998 08:27:25 Subject: Get 400 Search Engines, 5.75 (1) Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: <7tms_r@net.bio.net> Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: Apparently-To: *** LIMITED TIME SPECIAL OFFER *** For Only $5.75 (1) We Will Submit Your Web Site To Over 400 Of The Net's Hottest Search Engines, Directories & Indexes. If you're site isn't listed in the Search Engines, how can people find you to buy your products or services? * Over 90% of Internet Users Use Search Engines To Find Products & Services. If You're Not In Them, They Can't Find You. * Immediately Increase Your Sites Exposure * Your Competition Is Getting Noticed - Are You? Get Noticed By Your Prospects. Let us help you get the exposure you need. Let us take the work load off your back so you can get back to managing your business. We're the professionals that are here to help you have a shot at having a successful marketing experience with the internet. Visit Our Web Site To Learn More: http://www.tiffiny.com/sitesubmissions Thank You (1) The price for this service is $69 prepaid which covers the cost of submitting your site every three months for an entire year. We have shown the price of $5.75 to show you how inexpensive this program really is when the overall cost is annualized. Minimum 12 month term and full prepayment required. ====================================== Name removal requests. Send to: TO: webmaster@tiffiny.com SUB: remove ====================================== From BIOSCI-REQUEST Mon May 25 04:58:03 1998 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12-r-beta/8.6.6) id EAA28705; Mon, 25 May 1998 04:58:03 -0700 Received: from dux4.tcd.ie (dux4.tcd.ie [134.226.1.194]) by net.bio.net (8.6.12-r-beta/8.6.6) with ESMTP id EAA28702; Mon, 25 May 1998 04:58:01 -0700 Received: from 134.226.67.33 (sci67033.sci.tcd.ie [134.226.67.33]) by dux4.tcd.ie (8.8.7/8.8.7) with SMTP id MAA29455; Mon, 25 May 1998 12:55:56 +0100 (BST) Message-ID: <35695CD4.508A@tcd.ie> Date: Mon, 25 May 1998 12:58:12 +0100 From: Adrian Bracken Organization: Trinity College X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) MIME-Version: 1.0 To: rna@net.bio.net Subject: T7 transcribe from a fragment generated by a T7 start site primer????? X-URL: http://www.bio.net/hypermail/RNA/ Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit I am wondering if it is possible to: T7 a DNA fragment generated using a T7 start site primer or does T7 require sequence before the start site?? Please e-mail me at brackena@tcd.ie