From owner-schistosoma@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: (Dave Johnston) Newsgroups: bionet.organisms.schistosoma Subject: Schistosoma Genome Initiative Date: 11 Jul 1996 07:59:06 +0100 Lines: 73 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4s28nq$o5q@mserv1.dl.ac.uk> X-POPmail-Charset: British Original-To: schisto@dl.ac.uk Dear Schistosome Researchers, At the recent meeting of the WHO Schistosoma Genome Network it was agreed that all Network members would subscribe to the schisto newsgroup and would use it as a forum for announcements and requests relating to the Genome Initiative. This serves the treble purpose of (1) having a convenient, archived means of communication between all Network members (thank you, BIOSCI) (2) publicising the results of Network activity and the availabity of new protocols and resources to the wider schisto community (3) providing an easy way for the wider schisto community to contact Network members. We hope that this decision will encourage communication and the sharing of resources within the entire community and the exploitation of results from the Genome Initiative. Hi Paul, sorry for the delay in replying. I felt pretty jet-lagged also, finally touching base at 1.00am on Sunday morning. With respect to the clone names, Ian and I have put our heads together and suggest the following which we will circulate to schisto newsgroup next Wednesday. Hopefully everyone will be happy woth this (:-)) ABCCCCCC.DEF (UPPER CASE throughout) where: A=species (M=mansoni, J=japonicum, others yet to be coded) B=stage (A=adult, E=egg, L=miracidium (larvae), S=sporocyst, C=cercaria, L= lung, M=male adult, F=female adult etc) CCCCCC=unique clone identifier, freeform 6 characters (must use all 6) D=laboratory (R=Rene Rachou, N=NHM, T=TBRI, A=Ain Shams, M=UFMG, Q=QIMR, P= Inst. Pasteur etc) E=unique library identifier F=primer (3=T3, 7=T7, F=M13 forward, R=M13 reverse, S=SK, K=KS etc) I have written to NCBI to ask if there is anyway that existing submissions can be automatically changed via some kind of dataparsing script if we tell them how the old code relates to the new code. Hopefully they will say yes! With respect to your japonicum submissions, sorry but the second email (NCBI's response to your mailing) did not arrive so could you please send it again. I will be away for the next couple of days and back in next Wednesday. PS. We didn't get the Wellcome Trust Cyclin/Ras grant (*hit, oh *hit, oh * hit!) Best wishes, David DAJ David A. Johnston Executive Secretary to the WHO Schistosoma Genome Network, Researcher, Biomedical Parasitology Division, Dept of Zoology, The Natural History Museum, Cromwell Road, South Kensington, London SW7 5BD, England tel 0171 9389297 (from outside UK: 44 171 9389297) fax 0171 9388754 (from outside UK: 44 171 9388754) email daj@nhm.ac.uk From owner-schistosoma@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: (Dave Johnston) Newsgroups: bionet.organisms.schistosoma Subject: Schistosoma Genome Initiative- Proposed EST designation Date: 11 Jul 1996 08:27:58 +0100 Lines: 65 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4s2adu$q3a@mserv1.dl.ac.uk> X-POPmail-Charset: British Original-To: schisto@dl.ac.uk For the past 18 months, some of the labs involved in the WHO Schistosoma Genome Initiative's gene discovery effort have been using a standardised nomenclature for naming cDNA clones and the expressed sequence tag (EST) sequences derived from them. At a recent meeting of the WHO Genome Network, it was decided to revise this nomenclature so that the designation could be used as a file name under the DOS operating system (ie. limiting it to 8+3 letters long). Below is the proposed new nomenclature. clone = ABCCCCCC.DE EST derived from clone ABCCCCCC.DE = ABCCCCCC.DEF (UPPER CASE throughout) where: A=species (M=mansoni, J=japonicum, other species to be added as required) B=stage (A=adult, E=egg, L=miracidium (larvae), S=sporocyst, C=cercaria, L=lung, M= male adult, F=female adult, others to be added as required) CCCCCC=unique clone identifier (freeform 6 characters (must use all 6)) D=laboratory (A=Ain Shams University, Cairo; M=Federal University of Minas Gerias, Belo Horizonte; N=Natural History Museum, London; P= Institute Pasteur, Lille; Q=Queensland Institute for Medical Research, Brisbane; R=Centro de Pesquisas Rene Rachou, Belo Horizonte; T=Theodor Bilharz Research Institute, Cairo; others to be added as required) E=unique library identifier (to be asigned by the designating lab to distinguish between multiple libraries from the same life cycle stage) F=primer used to generate the EST (3=T3, 7=T7, F=M13 forward, R=M13 reverse, S=SK, K=KS, others to be added as required) Please mail comments and suggestions concerning this proposed new designation to the newsgroup for discussion. (Note to the EST producing Labs. Once we have decided on the final designation, NCBI have confirmed that they are happy to update the existing schisto ESTs on DBEST to reflect this new designation. All that they require is to be told how the old clone/EST names need to be altered to reflect the new designation and they will write data parsing scripts to do the updating automatically so that all the schisto records will have the same type of designation. I will arrange this for all the submitting labs.) DAJ David A. Johnston Executive Secretary to the WHO Schistosoma Genome Network, Researcher, Biomedical Parasitology Division, Dept of Zoology, The Natural History Museum, Cromwell Road, South Kensington, London SW7 5BD, England tel 0171 9389297 (from outside UK: 44 171 9389297) fax 0171 9388754 (from outside UK: 44 171 9388754) email daj@nhm.ac.uk From owner-schistosoma@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: (Dave Johnston) Newsgroups: bionet.organisms.schistosoma Subject: RE: Schistosoma Genome Initiative Date: 11 Jul 1996 09:30:20 +0100 Lines: 17 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4s2e2s$200@mserv1.dl.ac.uk> X-POPmail-Charset: British Original-To: schisto@dl.ac.uk Whoops, sorry about the unintended extra bit on the end of my initial message :-) DAJ David A. Johnston Executive Secretary to the WHO Schistosoma Genome Network, Researcher, Biomedical Parasitology Division, Dept of Zoology, The Natural History Museum, Cromwell Road, South Kensington, London SW7 5BD, England tel 0171 9389297 (from outside UK: 44 171 9389297) fax 0171 9388754 (from outside UK: 44 171 9388754) email daj@nhm.ac.uk From owner-schistosoma@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!anatomy.su.oz.au!kwadwo From: kwadwo@anatomy.su.oz.au (Kwadwo Appiah) Newsgroups: bionet.organisms.schistosoma Subject: (none) Date: 16 Jul 1996 16:46:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607162211.IAA02757@thor.anatomy.su.oz.au> NNTP-Posting-Host: net.bio.net unsubscribe schisto Kwadwo Appiah From owner-schistosoma@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!anatomy.su.oz.au!kwadwo From: kwadwo@anatomy.su.oz.au (Kwadwo Appiah) Newsgroups: bionet.organisms.schistosoma Subject: How do I unsubscribe? Date: 16 Jul 1996 01:24:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607160824.SAA29289@thor.anatomy.su.oz.au> NNTP-Posting-Host: net.bio.net Could someone tell me how to unsubscribe from this mail list please. Thanks in advance. Kwadwo. From owner-schistosoma@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!agate!spool.mu.edu!usenet.eel.ufl.edu!news.ultranet.com!homer.alpha.net!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: JAN CZEKAJEWSKI <75144.2413@CompuServe.COM> Newsgroups: bionet.mycology,bionet.organisms.schistosoma,bionet.plants,bionet.plants.education,bionet.toxicology Subject: Measure Ferment. O2/CO2 exchange Date: 19 Jul 1996 16:48:23 GMT Organization: Columbus Instruments Lines: 14 Message-ID: <4soe8n$gk4$3@mhadg.production.compuserve.com> Xref: biosci bionet.mycology:4385 bionet.organisms.schistosoma:144 bionet.plants:11983 bionet.plants.education:826 bionet.toxicology:828 We have developed fermentation gas exchange computer which measures precisely oxygen consumption and CO2 production in multiple fermentors. It employs one set of precision gas analyzers having 0.001% resolution. Differential measurements of gas (O2 and CO2 with optional H2S, CH4, H2) levels at the input and output of fermentor combined with gas mass flow measurements lead to precise measurement of mg O2 consumed and mg CO2 produced. Equipment can work with wide range of O2 and Co2 levels (0-100%) and can be used for both aerobic as well as anaerobic processes. It can be connected to variety of fermentors. If you like to receive description of this instrument, please e-mail your street address. JCZEK From owner-schistosoma@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.organisms.schistosoma Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 19 Jul 1996 02:00:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607190900.CAA25078@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-schistosoma@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!news.sol.net!newspump.sol.net!news.mindspring.com!news.sprintlink.net!news-dc-5.sprintlink.net!news.sprintlink.net!news-dc-9.sprintlink.net!news1.sunbelt.net!wofford.edu!holmanbm From: holmanbm@wofford.edu Newsgroups: bionet.organisms.schistosoma Subject: exit Date: 24 Jul 96 10:48:30 EST Organization: Info Avenue INTERNET Access Lines: 2 Message-ID: <1996Jul24.104830@wofford.edu> NNTP-Posting-Host: truth.wofford.edu here todo. here todo. mister i cant find my dog. can you help? From owner-schistosoma@net.bio.net Fri Jul 26 23:00:00 1996 Path: biosci!UCDAVIS.EDU!mpcarlos From: mpcarlos@UCDAVIS.EDU ("Maria Paz S. Carlos") Newsgroups: bionet.organisms.schistosoma Subject: SILCIBIO CONGRESO IN PUERTO RICO: REGISTRACION Y RESUMENES (CALL FOR ABSTRACTS) Date: 27 Jul 1996 11:22:12 -0700 Organization: University of California Lines: 277 Sender: daemon@net.bio.net Distribution: world Message-ID: <31FA612B.71B6@ucdavis.edu> Reply-To: mpcarlos@ucdavis.edu NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. --------------54A74EF92B2 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit PLEASE POST AND CIRCULATE THIS OFFICIAL ANNOUNCEMENT. WE LOOK FORWARD TO SEEING YOU IN PUERTO RICO. 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this newgroup already. I would like to know if there are any rapid diagnostic tests available for schistomoniasis, either for fecal testing or serological testing. My company is building a network of distributors for marketing rapid, inexpensive, easy-to-use diagnostic tests worldwide. I would greatly appreciate learning the current status of schisto diagnosis - what tests are available and whether these tests suffer from problems with specificity or sensitivity. Regards, Mark Hughes *************************************************** Mark Hughes, DVM, MS RapidTest Diagnostics International, Inc. 4524 Felter Road Milpitas, CA 95035 USA Phone/Fax: 510-441-RTDX (441-7839) E-mail: MHughes77@aol.com *************************************************** From owner-schistosoma@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Sean Ryan Newsgroups: bionet.general,bionet.jobs.wanted,bionet.organisms.pseudomonas,bionet.organisms.schistosoma,bionet.parasitology Subject: Irish First Class Hons. Parasitology Grad. for Ph.D Date: 31 Jul 1996 21:41:20 -0700 Organization: Trinity College, Dublin, Ireland Lines: 12 Sender: biohelp@net.bio.net Distribution: world Message-ID: <4tn8rd$57a@web3.tcd.ie> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.general:22883 bionet.jobs.wanted:7249 bionet.organisms.pseudomonas:142 bionet.organisms.schistosoma:149 bionet.parasitology:1686 I am a graduate of zoology from Trinity College Dublin with a first class hons degree and a specialisation in Parasitology and am now looking for a suitable Ph.D. subject involving the epidemiology/pathology of a parasite(s) of medical/veterinary importance. If anybody has a place for a Ph.D. student or knows of someone who has, I would appreciate an e-mail to kpryan@tcd.ie thanks..Sean Ryan ps replies from Australia/Europe especially welcome.