From owner-schistosoma@net.bio.net Tue Nov 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.organisms.schistosoma Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 25 Nov 1998 01:13:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 248 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73ghnh$q2p@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Dr.Christoph G.Grevelding Genetic Parasitology Institute of Genetic & Center for Biological and Medical Research Heinrich-Heine-Universitaet Universitaetsstrasse 1 40225 Duesseldorf Tel.:(49)-211-81-13070 Fax :(49)-211-81-12333 (49)-211-81-12279 e-mail: Christoph.Grevelding@uni-duesseldorf.de http://www.uni-duesseldorf.de/WWW/MathNat/Parasitologie/gen_para.htm From owner-schistosoma@net.bio.net Tue Nov 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: David DeMallie Newsgroups: bionet.organisms.schistosoma Subject: transmission electron micrographs Date: 25 Nov 1998 08:43:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73hc32$bt5@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for transmission electron micrographs of Shistosoma Mansoni for an upcoming exhibit at the American Museum of Natural History. Any help in finding TEMs would be greatly appreciated. Sincerely, David DeMallie (212) 769-5074 From owner-schistosoma@net.bio.net Tue Nov 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Gabbuti Andrea Newsgroups: bionet.organisms.schistosoma Subject: Research articles about neuroschistosomiasis Date: 25 Nov 1998 08:43:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73hc37$bti@net.bio.net> NNTP-Posting-Host: net.bio.net I am an italian doctor, and a patient of mine present. amaurosi. I need articles about lesion by schistosoma in CNS Thanks for the Help Andrea Gabbuti From owner-schistosoma@net.bio.net Tue Nov 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Dr. Christoph Grevelding" Newsgroups: bionet.organisms.schistosoma Subject: network problems Date: 25 Nov 1998 06:15:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73h3e7$mfa@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Schisto-members! Due to a total computer crash I had some serious problems in maintaining the network for some weeks. Now my computer and the net seem to work again. There are some messages which have been stored for a while due to this occurrence. I will forward them to you during the next hours. Sincerely, Christoph Grevelding (Moderator) From owner-schistosoma@net.bio.net Wed Nov 25 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "xjx1@canterbury.ac.uk" Newsgroups: bionet.organisms.schistosoma Subject: Seek for collaborator Date: 26 Nov 1998 00:48:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73j4lj$8ud@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all: A novel plant molluscicide is initial proven have a high potency to amphibious snail of intermediate host of S japonium, Oncomelania hupensis; fresh water snail of intermediate host of s. mansoni, Biomphalaria glabarate and the intermediate host of Fascioliasis, Lymnaea stagnalis. It hope to seek for collaborator to further research with Biomphalaria snail and Bulinus snail. If anyone has a interesting in this field, please reply this e-mail address. we could discuss in detail. With best regards, Xing-jian Xu Associate Professor ---------------------- xjx1@canterbury.ac.uk From owner-schistosoma@net.bio.net Wed Nov 25 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Dr. Christoph Grevelding" Newsgroups: bionet.organisms.schistosoma Subject: Schistosoma Genome Network - Informatics Training Opportunity Date: 26 Nov 1998 01:33:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 81 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73j79i$da0@net.bio.net> NNTP-Posting-Host: net.bio.net This is a message forwarded for David A. Johnston: Dear all, At the last Schisto Network Meeting, we made a recommendation that formal training in bioinformatics be arrange for the Network. The other Networks made similar recommendations and so Mark Blaxter applied to the WHO Genome Steering Committee for funds to hold a cross-network bioinformatics training Workshop. Funding was approved at the last Committeee Meeting. The plan is to hold the Workshop at the Hinxton Hall Genome Centre near Cambridge, UK (the home of the Sanger Centre, European Bioinformatics Institute and Wellcome Pathogen Genome Centre) from 29/03 1999 to 02/04 1999, with participants arriving 28/03. For each Network, funding will probably be available to send 3-4 scientists (including the Network Secretaries). Training will be provided by both external experts and members of the Parasite Genome community. We are, therefore asking for nominations from individuals in the Schisto Network who would like to attend the meeting. Potential Applicants should, ideally: (1) be from a laboratory in a disease endemic country (2) be reasonably computer literate (3) be from a laboratory that is actively working in schisto genome analysis (mapping or gene discovery) and (ideally) has reasonable internet conections (4) be able to demonstrate a future commitment to the schisto initiative (5) be prepared and able to pass on expertise gained at the Workshop to other schisto / parasite genome workers in their country. HOW TO APPLY Please send to me, by email, fax or post (a) a C. V. (b) 2 letters supporting your application (c) a covering letter describing how the training will be of benefit both to you and to the Schisto Genome Network. All applications will be considered by a "Committee" of Phil LoVerde (as Network Co-ordinator), Gui Correa de Oliviera (as PI of the main schisto EST initiative) and myself (as Network Secretary). Although the committee will accept all applications, they will favor applications from junior faculty/young research scientists establishing themselves in field of schistosome genomics and post genomics NOTE NOTE NOTE NOTE NOTE NOTE NOTE NOTE NOTE The closing date for applications is 30TH November 1998. With best wishes, David. David A. Johnston, Secretary to the WHO Schistosoma Genome Network, Biomedical Parasitology Division, Dept. of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, England, UK. Tel: 0171-938-9297 (from outside the UK: 44-171-938-9297) Fax: 0171-938-9297 / 9249 / 8754 (from outside the UK: 44-171-938****) eMail daj@nhm.ac.uk Please note the new WWW site address: http://www.nhm.ac.uk/hosted_sites/schisto/ The Biomedical Parasitology Division is a WHO Collaborating Centre for the identification of schistosomes and their snail hosts. From owner-schistosoma@net.bio.net Thu Nov 26 22:00:00 1998 Path: biosci!biosci!not-for-mail From: CC Queiroz Newsgroups: bionet.organisms.schistosoma Subject: Acid Activation Protocol for TGF-beta Detection Date: 27 Nov 1998 01:12:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <73lqdf$c1r@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Friends, I am currently trying to set up a protocol for circulating TGF-beta1 detection by using reagents from R&D Systems. I am aware that it is necessary to perform an acid activation of the sera samples before assaying, however, I could not find detailed protocol information about this acidic treatment, even trough the R&D company itself. I would really appreciate if someone could send me the detailed protocol for acid activation of sera samples or inform some reference where I would be able to get it. In addition, if is this possible, since I am also aware that some researchers do not acidify the samples prior assaying for TGF-beta, I would like to know which assay is more accurate and why. Thank you very much in advance, Clo ---------------------------------------------------- Claudia Carvalho Queiroz Visiting Graduate Student Dep. of Biology, University of York, Heslington, York Y01 5DD, UK ccq1@york.ac.uk ---------------------------------------------------- From owner-schistosoma@net.bio.net Mon Nov 30 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Albert Kaltenberg Newsgroups: bionet.organisms.schistosoma Subject: Postdoctoral Position (schistosoma mansoni) in Duesseldorf / Germany Date: 30 Nov 1998 23:50:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <740742$l52@net.bio.net> NNTP-Posting-Host: net.bio.net A postdoctoral position (BAT II a) is available in a laboratory working on Molecular Parasitology and Signal Transduction http://www.uni-duesseldorf.de/WWW/MathNat/Parasitology/ The group in Duesseldorf is working on signal transduction molecules in the human parasitic worm Schistosoma mansoni. These molecules govern the maturation program of vitelline cells in females, an essential developmental process that is induced by pairing with the male. After identification of a couple of female-specifically expressed genes whose activity is regulated by the male, the new project concerns techniques that have been successfully used in signal transduction research during the last few years. The program tries to identify further signalling molecules that react with known molecules by protein-protein interaction.The methods include the yeast two-hybrid system, cloning of receptor targets and others. The final goal is to elucidate the signalling pathway that regulates vitelline cell proliferation and differentiation in female schistosomes and to open ways to inhibit their action. Literature: Grevelding, C.G., Sommer, G., and Kunz, W. (1997). Female-specific gene expression in Schistosoma mansoni is regulated by pairing. Parasitology 115, 635-640. Schuessler, P., Grevelding, C.G., and Kunz, W. (1997). Identification of Ras, MAP kinases, and a GAP protein in Schistosoma mansoni by immunoblotting and their putative involvement in male-female interaction. Parasitology 115, 629-634. Information: Prof. Dr. Werner Kunz Section of Genetic Parasitology Institute for Genetics, Heinrich-Heine-University, Universitaetsstr.1, D-40225 Duesseldorf, Germany Tel.+ Fax: (49)211-81-12333 E-mail: Werner.Kunz@uni-duesseldorf.de