From owner-schistosoma@net.bio.net Mon Aug 02 10:48:00 1999 Path: biosci!biosci!not-for-mail From: Bernd Kalinna Newsgroups: bionet.organisms.schistosoma Subject: PhD position (English version) Date: 2 Aug 1999 04:48:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <7o40hv$gdu@net.bio.net> NNTP-Posting-Host: net.bio.net Humboldt-Universität zu Berlin Lehrstuhl für Molekulare Parasitologie AG Schistosomen PhD position (Salary: BAT IIa/2, German Research Council) to work in the following project areas: - Studies on the function of Cathepsin D of Schistosoma mansoni utilising transgenic worms. The role of Cathepsin D will be studied with reverse genetics (transgenic worms, knock-outs, inducible expression) and biochemical methods (fluorometrie). Aim of the study is also to validate Cathepsin D as a therapeutic target. - Isolation und characterisation of the promotor of the schistosomal aspartic protease To express the transgen in Schistosomes it is important to characterise promotor- and regulatory sequences of the aspartic protease gen. In this project a genomic DNA library of S. mansoni will be screened with specific probes, clones sequenced and positive clones characterised further. - Protective potential of the aspartic protease of Schistosoma mansoni Recombinant protein (insect cell system) and DNA-vaccine vectors will be used to immunise laboratory animals. The humoral and cellular immune responses will be characterised. Prerequisites: Strong base in Molecular Biology, Biochemistry, Parasitology or Immunology. Information and applications: Humboldt-Universität zu Berlin, Lehrstuhl für Molekulare Parasitologie, AG Schistosomen, Philippstr.13, 10115 Berlin Dr. Bernd Kalinna Tel: 030-2093-6055 Fax: 030-2093-6051 email: bernd.kalinna@rz.hu-berlin.de http://www.biologie.hu-berlin.de/molpara/web/index.html From owner-schistosoma@net.bio.net Thu Aug 12 07:18:00 1999 Path: biosci!biosci!not-for-mail From: Jeff Bailey Newsgroups: bionet.organisms.schistosoma Subject: S. mansoni DNA isolation from human stools Date: 12 Aug 1999 01:18:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <7ou000$3g6@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Schisto Researchers, We are currently attempting to isolate S. mansoni egg DNA from human stool samples through a two-step process of initial egg purification followed by DNA extraction. Unfortunately the eggs we do purify contain contaminating fecal material and the DNA preparations show poor amplification due to PCR inhibitors (as well as possibly degraded DNA). I would appreciate any advice on how to better isolate the eggs or the DNA. Thank you, Jeff P.S. For the DNA preps, I have been using a Qiagen QIAamp kit to remove some of the PCR inhibitors. Although I have not directly done the egg isolations, I believe we use several initial filter steps and then a sucrose gradient. -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- Jeff Bailey Graduate Student work: 216-368-0834 Chakravarti Lab fax: 216-368-5857 2109 Adelbert Rd e-mail: jab@po.cwru.edu Cleveland, OH 44106 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= From owner-schistosoma@net.bio.net Tue Aug 24 06:49:00 1999 Path: biosci!biosci!not-for-mail From: Carl Zimmer Newsgroups: bionet.organisms.schistosoma Subject: Looking for SEMs of adult schistosomes Date: 24 Aug 1999 00:49:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: greveld@uni-duesseldorf.de Distribution: world Message-ID: <7ptipp$41r@net.bio.net> NNTP-Posting-Host: net.bio.net Greetings: I'm currently working on a book about parasites intended for the general audience. I'm looking for SEMs of the parasites I discuss in the book, both for interior illustrations and for a collage on the cover. I'm particularly interested in SEMs of adult schistosomes. I'd be grateful to communicate with any researchers who have images they think would be suitable, or tips on other people who might have them. Thanks for your time Best wishes Carl Zimmer 41-15 45th St., Apt. 2J Sunnyside, NY 11104 USA zimmer@panix.com phone: (718) 729-7452 fax: (603) 452-1666