From owner-schisto@hgmp.mrc.ac.uk Fri Sep 1 08:10:14 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3EFE917A5C; Fri, 1 Sep 2000 08:10:13 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 32BE417A89; Fri, 1 Sep 2000 08:10:10 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: Nick Smith Date: Thu, 31 Aug 2000 20:18:13 -0700 Subject: 8th International Coccidiosis Conference Message-Id: <20000901071010.32BE417A89@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Dear Parasitologist, I am pleased to announce the launch of the website for the combined meeting of the 8th International Coccidiosis Conference and the Annual Meeting of the Australian Society for Parasitology in Palm Cove, Cairns, in Australia's tropical north from July 9-13, 2001. The website will be updated regularly with new information, including announcements for registration and abstract submission (the Second Announcement will be placed on the website in October, 2000). Please look in on us regularly ! The Website can be found at : http://www.science.uts.edu.au/depts/cmb/mpu/conference.html Best wishes, Nick Dr Nick Smith Molecular Parasitology Unit Tel. ++61 2 9514 4013 Department of Cell and Molecular Biology FAX ++61 2 9514 4026 University of Technology, Sydney Email Nick.Smith@uts.edu.au Westbourne St, Gore Hill, NSW, 2065, Australia From owner-schisto@hgmp.mrc.ac.uk Wed Sep 6 08:28:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 6306217A62; Wed, 6 Sep 2000 08:28:39 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3002317A68; Wed, 6 Sep 2000 08:28:38 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: David.Szczebak@state.ma.us ("Szczebak-FWE, David" (David Szczebak)) Date: Tue, 05 Sep 2000 23:11:47 +0100 Subject: schisto question Message-Id: <20000906072838.3002317A68@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Hello parasitologists, A possibly ignorant question from someone who knows next to nothing about schistosomiasis (excuse me for using this forum): What are the consequences from a human injesting schistosome eggs or larvae (miracidae?), or for that matter, actual flukes (cercaria?)? Can someone contract schisto. from drinking water contaminated with any of the above? After having contracted it and while researching schisto. I was unable to find any mention of this. David Szczebak From owner-schisto@hgmp.mrc.ac.uk Thu Sep 7 08:17:16 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 28FD617A93; Thu, 7 Sep 2000 08:17:15 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 245B217AEF; Thu, 7 Sep 2000 08:17:14 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: cromero@iq.usp.br ("Celso") Date: Wed, 06 Sep 2000 18:41:04 +0100 Subject: weight of schisto worm Message-Id: <20000907071714.245B217AEF@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Hello all, Please, I need data about weight of schistosoma mansoni worm total weight (average for males and females). Exist such information? In another hand, I looking information about PCR and RT-PCR protocols for individual schistosoma woms. If you have any experience in this field, please help me. Many Thanks Celso Romero From owner-schisto@hgmp.mrc.ac.uk Thu Sep 7 09:07:41 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7912D17B78; Thu, 7 Sep 2000 09:07:40 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5627117B7A; Thu, 7 Sep 2000 09:07:39 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: Date: Thu, 07 Sep 2000 08:42:22 +0100 (BST) Subject: Re: weight of schisto worm Message-Id: <20000907080739.5627117B7A@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Celso, in our experience, the wet-weight of 10 worms is about 0.2g (mixed sex). In the past, we developed a Direct-PCR protocol using total worms or even total larval stages without prior DNA extraction. In the meantime this protocol has been used by Helene Monet from Perpignan (France) and Martin Kalbe from Erlangen (Germany) successfully. However, we have not yet tried to use this protocol for RT-PCR which might be difficult. For RT-PCR, you should consider to extract the RNA from individual worms using common kits like RNAeasy from Qiagen or the Dynabead magnetic-particle procedure (Dynal). Both work well in our hands. Here are some references which might be helpful in this respect: 1: Grevelding, C.G., Kampkoetter, A. and Kunz, W. (1997) Schistosoma mansoni: PCR on adult and larval stages without DNA extraction. Experimental Parasitology 85, 99-100 2: Grevelding, C.G., Kampkoetter, A., Hollmann, M., Schaefer, U. and Kunz, W. (1996) Direct PCR on fruitflies and blood flukes without prior DNA isolation. Nucleic Acids Research 24, 4100-4101 3: Schuessler, P., Gohr, L.G., Sommer, S., Kunz, W. and Grevelding, C.G. (1995) A simple method for the combined isolation of nucleic acids and protein from minor amounts of tissue. Trends in Genetics 11, 378-379 Sincerely, Christoph G. Grevelding >Hello all, > >Please, I need data about weight of schistosoma mansoni worm total weight >(average for males and females). Exist such information? > >In another hand, I looking information about PCR and RT-PCR protocols for >individual schistosoma woms. If you have any experience in this field, >please help me. > >Many Thanks > >Celso Romero From owner-schisto@hgmp.mrc.ac.uk Thu Sep 7 15:03:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id EBBE617A9B; Thu, 7 Sep 2000 15:03:39 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 10F9917A9E; Thu, 7 Sep 2000 15:03:37 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: TRISTEN775@aol.com Date: Thu, 07 Sep 2000 07:52:02 -0400 (EDT) Subject: Re: weight of schisto worm Message-Id: <20000907140337.10F9917A9E@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Dr. Celso: I think you will find the S. mansoni weight data you seek in the following article. Good luck in your work. If you need more information, it may be in my thesis written at Johns Hopkins in 1969. Sorry, can't help with the PCR questions. The mean weight of freeze dried, individually weighed male S. mansoni worms taken from a mouse at 45-43 days post infection was 87.4 micrograms, for females 26.4 micrograms. J Parasitol 1972 Jun;58(3):489-94 Related Articles, Books Changes in dry weight and glycogen content as criteria for measuring the postcercarial growth and development of Schistosoma mansoni. Lennox RW, Schiller EL PMID: 5042056, UI: 72230417 Robert W. Lennox, Sc.D. 4024 North Stuart Street Arlington, Virginia 22207 (703) 532-8587 tristen775@aol.com From owner-schisto@hgmp.mrc.ac.uk Tue Sep 26 08:24:49 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1093417B3B; Tue, 26 Sep 2000 08:24:47 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id A6D7517B3C; Tue, 26 Sep 2000 08:24:46 +0100 (BST) To: schisto@net.bio.net Newsgroups: bionet.organisms.schistosoma From: hongsj@cau.ac.kr (Sung-Jong Hong) Date: Tue, 26 Sep 2000 03:06:42 +0100 Subject: GST assay Message-Id: <20000926072446.A6D7517B3C@mercury.hgmp.mrc.ac.uk> Sender: owner-schisto@hgmp.mrc.ac.uk Precedence: bulk Dear schisto members, I assay an enzymatic activity of recombinant glutathione S-transferase (GST) for substrates. It is hard to find an extinction co-efficient for cumene hydroxide in papers available for me. Please, is there anybody who has experience with this topic and could help? Sincerely yours, Sung-Jong Hong Professor Hong Sung-Jong Chung-Ang University Faculty of Medicine;Department of Parasitology 221 Heukseok-dong;Tongjak-gu;Seoul;156-756;Korea tel;fax:+82 (2) 826-1123 tel;work:+82 (2) 820-5683