A postdoctoral position is available in the laboratory of Doris Wagner
at the University of Pennsylvania in Philadelphia
(http://www.bio.upenn.edu/faculty/wagner/) effective immediately for a
highly motivated scientist interested in studying *the role of chromatin
remodeling ATPases* in plant development.
The three-year funded position is aimed at identification of the direct
targets of the SWI/SNF chromatin remodeling ATPase SPLAYED through
global binding studies (ChIP on chip) and at elucidating the changes in
nucleosome position and/or occupancy that result from the activity of
SPLAYED and related ATPases. The Wagner laboratory is a highly
interactive group of scientists located in a brand new Biology building
on the vibrant University of Pennsylvania campus, which features a
strong chromatin research community.
The ideal applicant should have prior experience with molecular biology
techniques (ChIP, real-time PCR) . Knowledge of plant development is
desirable. If you are interested in this position, please send (by
e-mail) a coverletter stating your research interests, a curriculum
vitae, and names as well as e-mail addresses of three references to:
Doris Wagner
wagnerdo from sas.upenn.edu
/_Related Publications:_/
Kwon, C. S. and Wagner, D. (2007). Unwinding chromatin – a few genes at
a time. Trends
Genet.
Bezhani, S., et al. (2007). Unique, shared and redundant roles for the
Arabidopsis SWI/SNF chromatin remodeling ATPases BRAHMA and SPLAYED.
Plant Cell 19, 403-416.
Kwon, C.S., et al. (2006). A role for chromatin remodeling in regulation
of CUC gene expression in the Arabidopsis cotyledon boundary.
Development 133, 3223-3230.
Su, Y., et al. (2006). The N-terminal ATPase AT-hook-containing region
of the Arabidopsis chromatin-remodeling protein SPLAYED is sufficient
for biological activity. Plant J 46, 685-699.
Kwon, C.S., Chen, C., and Wagner, D. (2005). WUSCHEL is a primary target
for transcriptional regulation by SPLAYED in dynamic control of stem
cell fate in Arabidopsis. Genes Dev 19, 992-1003.
Wagner, D. (2003). Chromatin regulation of plant development. Curr Opin
Plant Biol 6, 20-28.
/_Background:_/
Chromatin physically restricts accessibility of the information encoded
in the genome to regulatory proteins such as transcription factors.
Chromatin remodeling ATPases use the energy derived from ATP hydrolysis
to change genome accessibility by altering nucleosome position or by
transiently removing nucleosomes. The in vivo roles of these remodelers,
including those of the important SWI/SNF family, were recently described
using viable SWI/SNF ATPase null mutants in plants. These, as well as
genome wide expression studies have implicated chromatin remodeling
ATPases in the regulation of many important developmental pathways. In
each pathway they appear to regulate expression of very few key
developmental regulators.
--
Doris Wagner
Department of Biology, 103G Lynch
University of Pennsylvania wagnerdo from sas.upenn.edu
415 S. University Ave Phone: (215) 898-0483
Philadelphia, PA 19104-6018 Fax: (215) 898-8780
