Hi,
We've read all of the papers about Tnt and Tto1 being used as an agent
for mutagenesis in several crops, and are anxious to try this in other
species.
Unfortunately we have had zero luck obtaining these clones from the
laboratories that have published them. Despite countless emails we
cannot procure these materials.
Today we ordered the primers to amplify and clone them from tobacco
ourselves, but I thought a note to the community might find some
success.
Thank you for your assistance, we appreciate any advice you might have.
Kevin Folta
Kevin M. Folta
Assistant Professor
Horticultural Sciences Department and
Plant Molecular and Cellular Biology Program
PO Box 110690, 1301 Fifield Hall
Gainesville FL 32611
phone: 352-392-1928 ext 269 office
ext 270 lab
fax: 352-392-5653
www.arabidopsisthaliana.com
pmcb.ifas.ufl.edu <http://pmcb.ifas.ufl.edu/>