500ug/ml Carbenicillin or 200ug/ml Cefotaxin in the MS plate will kill
the agrobacteria, without harming the plants.
Good luck!
Morgan
----- Original Message -----
From: arab-gen-request from oat.bio.indiana.edu
Date: Thursday, April 26, 2007 1:01 pm
Subject: Arab-gen Digest, Vol 24, Issue 16
> Send Arab-gen mailing list submissions to
> arab-gen from net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/arab-gen
> or, via email, send a message with subject or body 'help' to
> arab-gen-request from net.bio.net
>
> You can reach the person managing the list at
> arab-gen-owner from net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Arab-gen digest..."
>
>
> Today's Topics:
>
> 1. MS Plate Contamination (Heather Babka)
> 2. POSTDOCTORAL POSITION (Sophia Stone)
>
>
> -------------------------------------------------------------------
> ---
>
> Message: 1
> Date: Tue, 24 Apr 2007 16:40:37 -0500
> From: "Heather Babka" <hbabka from iastate.edu>
> Subject: [Arabidopsis] MS Plate Contamination
> To: arab-gen from magpie.bio.indiana.edu
> Message-ID:
> <479139e20704241440h3e3c7589u29620ec0cceeaa64 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hello,
>
> I am trying to select T1 plants on MS plates with 30ug/mL
> Hygromycin. It
> seems that all of this newly collected seed is contaminated with
> bacteria(Agro?). Is there anything I can add to the media that
> would kill the
> Agrobacteria, but not harm the plants? Currently I sterilize with
50%
> Bleach + 0.02% Triton. I tried adding a 70% Ethanol wash but that
> did not
> help.
>
> Thanks,
> Heather
>
> --
> Heather L. Babka
> Interdepartmental Genetics
> Department of Genetics, Development, and Cell Biology
> 433 Bessey Hall
> Iowa State University
> Ames, IA 50011
> Phone: (515) 294-3509
> Fax: (515) 294-1337
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 25 Apr 2007 11:57:26 -0300
> From: Sophia Stone <Sophia.Stone from DAL.CA>
> Subject: [Arabidopsis] POSTDOCTORAL POSITION
> To: arab-gen from magpie.bio.indiana.edu
> Message-ID: <20070425115726.40d3bhssgiskgcoc from my1.dal.ca>
> Content-Type: text/plain; charset=ISO-8859-1
>
> POSTDOCTORAL POSITION
> Dalhousie University, Halifax, NS Canada
>
> A postdoctoral position is available starting September 2007 to
> study the
> biochemistry and molecular biology of the ubiquitination
> proteasome pathway in
> Arabidopsis thaliana. Successful applicants will join a new
> research group in
> the Biology department at Dalhousie University currently using a
> variety of
> techniques to define the pathway's role in plant development and
> response to
> stress. Candidates must have a strong background in protein
> biochemistry and
> molecular biology techniques. Previous experience in working with
> Arabidopsisis advantageous but not required. Salary will be
> commensurate with experience.
> Interested persons should send a CV and three letters of
> recommendation to Dr.
> Sophia Stone, Department of Biology, Dalhousie University, 1355
> Oxford Street
> Halifax, NS Canada B3H 4J1; email- Sophia.Stone from dal.ca or contact
> her at
> 902-494-4541 (http://biology.dal.ca/us/f/stone/stone.html).
>
>
> Sophia Stone
> Assistant Professor
> Department of Biology
> Life Science Centre
> Dalhousie University
> 1355 Oxford Street
> Halifax, Nova Scotia
> Canada B3H 4J1
> Email: Sophia.Stone from dal.ca
> Phone: 902-494-4541
> Fax: 902-494-3736
>
>
>
> ------------------------------
>
> _______________________________________________
> Arab-gen mailing list
> Arab-gen from net.bio.net
> http://www.bio.net/biomail/listinfo/arab-gen
>
> End of Arab-gen Digest, Vol 24, Issue 16
> ****************************************
>
