Hi Stefan,
you can obtain high quality RNA from etiolated seedlings using Qiagen
columns. The yield is low particularly if you grow your seedlings in
the absence of sucrose (e.g. 1/2X MS is what we typically do). Don't
grow your seedlings for more than 4-5 days (5 days is really the
maximum, I prefer 3-4 days)
in our hands what works best is to extremely quickly (under safe green
light) harvest and grind the etiolated seedlings under liquid N2 using
a mortar and pestle (pre-chilled using liquid N2). This fine powder
can then be stored at -70°C for short periods of time, we get best
results by immediately processing it to get RNA.
good luck Christian
--
Christian Fankhauser
CIG
Genopode Building
University of Lausanne
CH-1015 Lausanne
Phone ++41 21 692 3941
++41 21 692 3920 (Nathalie Clerc, administrative
assistant)
FAX ++41 21 692 3925
[1]http://www.unil.ch/cig/page8391_en.html
References
1. http://www.unil.ch/cig/page8391_en.html
