[Arabidopsis] RE: RNA extraction from etiolated seedlingsArab-gen Digest, Vol 27, Issue 9

[Wang XW]

Dear Stefan,

I also used the same kit (qiagen) and from same etiolated seedlings. And
It works well well.

I suggest you check:

1. low teperature extraction?
2.DEPC -treated water
3.exact seedlings (weight) the Kit required



Mr Xuewen Wang (PhD)
 
School of Biology
 Royal Holloway, Uni of London
Surrey UK
TW20 0EX
email: x.w.wang from rhul.ac.uk
 
 
 


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Today's Topics:

   1. RNA extraction from etiolated seedlings (Stefan Weinl)


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Message: 1
Date: Tue, 17 Jul 2007 11:05:45 +0200
From: Stefan Weinl <Stefan.Weinl from online.de>
Subject: [Arabidopsis] RNA extraction from etiolated seedlings
To: arab-gen from magpie.bio.indiana.edu
Message-ID: <469C8669.1010901 from online.de>
Content-Type: text/plain; charset=ISO-8859-15; format=flowed

We are in a process to built our own cDNA library and therefore we are 
trying to isolate RNA from etiolated seedlings. Up to now we tried 
column-based kits (Qiagen and Promega) and used TriFast solution to 
isolate RNA but did not receive suitable RNA.
Now we would like to use a classical protocol using phenol and LiCl. We 
would be very thankful for a protocol which works for etiolated 
seedlings, or any suggestions regarding RNA extraction from etiolated 
material.

Thank you in advance,

Stefan




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