From jmalonso from unity.ncsu.edu Tue Jul 1 08:41:51 2008 From: jmalonso from unity.ncsu.edu (Jose Alonso) Date: Tue Jul 1 13:01:00 2008 Subject: [Arabidopsis] broad host-rage vector Message-ID: Does anybody have an Arabidopsis (Col) cDNA expression library in a broad host-range vector? I would like to express Arabidopsis proteins in Gram(-) strains other than E. coli. Thanks, Jose Jose Alonso Phone: (919)515 5729 Fax: (919) 515 3355 Email: jmalonso@unity.ncsu.edu Web: http://www4.ncsu.edu/~jmalonso From ulrike.mayer from zmbp.uni-tuebingen.de Wed Jul 2 09:04:37 2008 From: ulrike.mayer from zmbp.uni-tuebingen.de (Ulrike Mayer) Date: Thu Jul 3 18:11:24 2008 Subject: [Arabidopsis] RFP anti mouse antibody Message-ID: <486B8AF5.2060009@zmbp.uni-tuebingen.de> Hi everybody, Does anybody know of a RFPanti mouse antibody which works in Western and immunos in Arabidopsis? Thanks for telling, Ulli -- Dr. Ulrike Mayer-Juergens University of Tuebingen ZMBP - Developmental Genetics Auf der Morgenstelle 3 72076 Tuebingen Tel.: ++49-7071/29-78887 Fax: ++49-7071/29-579 ulrike.mayer@zmbp.uni-tuebingen.de http://www.zmbp.uni-tuebingen.de/DevelopmentalGenetics From lab27 from unitus.it Wed Jul 2 05:31:50 2008 From: lab27 from unitus.it (lab27@unitus.it) Date: Thu Jul 3 18:11:29 2008 Subject: [Arabidopsis] XXth International Congress on Sexual Plant Reproduction Message-ID: <1b2ec5dc-71a5-4d7e-95cc-94f4ab936395@l42g2000hsc.googlegroups.com> XXth International Congress on Sexual Plant Reproduction Brasilia-DF , Brazil ---- August 4 - 8th, 2008 Registration sold – lower rate Dear colleague, I registered for the Congress at the regular fare of 620 - US$ but recently realized that it was not possible to me to attend the meeting. Because the cancelation policy does not allow any refund, my only possibility to recover (part of) my registration fee is to transfer my registration to someone that still have to pay. So this is to offer to you this possibility that will be helpful for both (for me to recover 500 US$ is enough). Of course I do have the availability of the Organizing Committee to make this change. If you are interested, pleased, do not hesitate to contact me ASAP (Andrea Mazzucato, mazz@unitus.it) From eurus from psb.ugent.be Wed Jul 2 06:54:41 2008 From: eurus from psb.ugent.be (Eugenia Russinova) Date: Thu Jul 3 18:11:33 2008 Subject: [Arabidopsis] job ad Message-ID: <486B6C81.3090809@psb.ugent.be> *Two 3-years PhD positions* are available in the lab of Jenny Russinova* (*VIB/Department of Plant Systems Biology/University of Ghent, Ghent, Belgium) *funded by the *European ITN FP7 Project BRAVISSIMO (BRAssinosteroid Venture Increasing StudentS' International MObility). The aim of the proposed research is to study the trafficking of membrane proteins in the model plant Arabidopsis. The projects will focus on functional analysis of the endocytosis of brassinosteroid receptor complex and further understanding of its significance for brassinosteroid signal transduction and plant growth. We are looking for a motivated and hard working young scientist with a solid background in cell and molecular biology, basic skills in biochemistry, a deep interest in receptor signalling and cell growth, and a nice and collegial working spirit. Experience with plants, /Arabidopsis/, GFP and microscopy is an advantage but not required. *_Profile:_* The applicants must have a master degree or equivalent university diploma in biology, molecular biology or biochemistry and be in the first four years (full-time equivalent) of their research careers, starting at the date of obtaining the degree which would formally entitle them to embark on a doctorate. The positions are available for non-Belgium nationalities only Good knowledge in English is obligatory. Good communication and writing skills are a necessity *_Contact:_* Send your motivation letter, CV and names of two referees by email to: Jenny Russinova (eurus@psb.ugent.be) -- ================================================================== Jenny Russinova, PhD Tel:+32 (0)9 331 39 31 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM eurus@psb.ugent.be http://www.psb.ugent.be ================================================================== From ericl89 from hotmail.com Wed Jul 2 10:39:30 2008 From: ericl89 from hotmail.com (Eric Lam) Date: Thu Jul 3 18:11:38 2008 Subject: [Arabidopsis] Postdoc Position in Chromatin and Epigenetics Message-ID: A POSTDOCTORAL POSITION is available at the Biotech Center, Rutgers Univers= ity, in the laboratory of Prof. Eric Lam (http://aesop.rutgers.edu/~lamlab)= for a highly motivated scientist interested in studying chromatin organiza= tion and global regulation of genes via epigenetic control. Recently, we h= ave established a set of about 300 mapped lines of novel transposants in Ar= abidopsis with single Ds insertions that allows functional and structural c= haracterization of global epigenetic control in living plants. This unique= resource promises to reveal interesting new insights to the complex networ= k of the epigenome using Arabidopsis as a paradigm. The successful applica= nt will apply this collection of lines to examine global epigenetic control= as a function of development and in response to stresses. In addition, th= ese lines will also be used to screen for novel chemicals that can regulate= epigenetic pathways using chemical biology approach. =20 The ideal applicant should have prior experience with molecular (ChIP a= ssay, PCR, cloning, northern/Southern blotting, etc.) and biochemical (west= ern blotting, enzyme assay, etc) techniques, advanced microscopy, and/or ch= emical biology approaches. Knowledge of epigenetic mechanisms is desirable = but not required. If you are interested in this position, please email a co= ver-letter stating your research interests, a curriculum vitae, and contact= information for three references to: Prof. Eric Lam, ericL89@hotmail.com The Biotech Center at Rutgers University is located in New Brunswick, N= ew Jersey, and enjoys a convenient location close to the famous Jersey Shor= e and Pocono Mountains while also easily accessible (within 1 hour by car o= r train) to metropolitan areas such as New York City and Philadelphia. The= Biotech Center at the School of Environmental and Biological Sciences in t= he Cook Campus is well-equipped with advanced instrumentations for molecula= r biology, biochemistry and microscopy. Rutgers University is an equal opp= ortunity employer that strongly encourages underrepresented groups to apply= for open positions. =20 Related Publications: "Genome-wide transposon tagging reveals location-dependent effects on trans= cription and chromatin organization in Arabidopsis" Plant J. Published On= line: Apr 12 2008; DOI: 10.1111/j.1365-313X.2008.03517.x =93DNA hypomethylation reduces homologous pairing of inserted tandem repeat= arrays in somatic nuclei of Arabidopsis thaliana=94 Plant J. 2005 Nov;44(= 4):531-40. "Visualizing chromosome structure/organization" Annual Rev. of Plant Biolo= gy, 2004; 55: 537-554. "Chromatin of endoreduplicated pavement cells has greater range of movement= than that of diploid guard cells in Arabidopsis thaliana" J. of Cell Scie= nce, 2003; 116: 2195-2201. "Detection of green fluorescence protein tagged chromosomes in live Arabido= psis thaliana" Genome Biology, 2001; 2: 0045.1-0045.10. Prof. Eric Lam=20 Biotechnology Center for Agriculture and the Environment Professor of Plant Science,=20 Rutgers, The State University of New Jersey, U.S.A. = From campanellj from mail.montclair.edu Tue Jul 1 18:34:50 2008 From: campanellj from mail.montclair.edu (James J. Campanella) Date: Thu Jul 3 18:11:41 2008 Subject: [Arabidopsis] pCambia1201 question Message-ID: Dear fellow workers in the mines of science, I have been tryingto work with the pCambia1201 plasmid for the lastseveral weeks, but ithas been driving my lab a bit crazy. My problemis that it does not wantto digest with most endonucleases we've tried.I simply can not use theplasmid if I can't cut it, so I have aproblem. It was suggested to meto try transferring the plasmid to DH5cells, which I did to no avail--the resultant plasmid still does notcut with almost every enzyme in mylibrary. I was finally able to getit to cut with XhoI and PvuII, buteven those were only partialdigests. Does anybody have anyadvice on working with this thing? The literaturedoes not suggest thatthere is even a problem, and the group thatengineered it could onlysuggest "trying all your enzymes until youfind one that cuts", which isa bit impractical. Thanks for any help, Jim Campanella ------------------- James J. Campanella, Associate Professor, Department of Biology and Molecular Biology Montclair State University 1 Normal Avenue Montclair, NJ 07043 Alternate email address: jcamp@alumni.uchicago.edu Ph: 973-655-4097 Fax: 973-655-7047 From cdouglas from interchange.ubc.ca Thu Jul 3 17:45:18 2008 From: cdouglas from interchange.ubc.ca (Carl Douglas) Date: Thu Jul 3 18:11:46 2008 Subject: [Arabidopsis] Postdoctoral positions at The University of British Columbia Message-ID: Poplar biofuels: Postdoctoral positions in bioinformatics, genomics, molecular biology, and biochemistry University of British Columbia, Vancouver BC, Canada Six postdoctoral positions in bioinformatics, genomics, molecular biology, and biochemistry are available as part of a recently funded Genome British Columbia Applied Genomics Innovation Program (AGIP) project ?Optimized Populus feedstocks and novel enzyme systems for a British Columbia bioenergy sector?. Positions will be for two to three years, starting in the Fall of 2008, and will be located at the University of British Columbia. This multi-investigator project is centered at the University of British Columbia (Departments of Botany, Wood Science, Forest Sciences, the Centre for Plant Research, and the Michael Smith Laboratories), with collaborators at the University of Victoria, and the support of technology platforms at the Vancouver Genome Sciences Centre, Prostate Centre Microarray Facility, and Centre for Molecular Medicine and Therapeutics (CMMT). International collaborators include the US Department of Energy, USDA Forest Service, and Umea Plant Science Center. If interested, please forward a cover letter indicating the position you are interested in, a CV, and the names of three references via email with the subject ?AGIP Biofuels Position? to: Carl Douglas, Project Leader AGIP Biofuels Department of Botany University of British Columbia Vancouver BC V6T 1Z4 CANADA E-mail: cdouglas@interchange.ubc.ca Postdoctoral Fellow position - Bioinformatics for population genomics As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will pioneer whole genome SNP discovery using massive parallel sequencing (Solexa) data. You will coordinate the assembly of short read data by bioinformatics staff at the Genome Sciences Centre, Vancouver. You will be responsible, together with the project database bioinformatician, for pipelining and databasing the SNPs. You will also be responsible for analytical work on the SNP variation patterns, including detection of the signature of selection and QTN detection, and for targeting 6000 SNPs of interest for expanded sampling using Illumina SNP detection platforms. The position represents a superb opportunity to work at the leading edge of population genomics and your work will underpin a major tree biology project. Experience with one or more of the following would be an advantage: next generation sequencing, short read assembly, perl programming, database management, statistical genetics, SNP detection assays and selection detection algorithms. Postdoctoral Fellow - Massive parallel sequencing, SNP detection and association genetics. As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will be responsible for the genome resequencing of trees from natural populations using chip-capture technology and Solexa sequencing. Your data will be analyzed and pipelined by bioinformatics colleagues. You will also be responsible for large-scale SNP assays involving >6000 SNPs and hundreds of trees using the Illumina Golden Gate system, and in collaborating with phenotypers and bioinformaticians to perform large scale association analyses with an extensive range of phenotypic traits. You will have access to state-of-the-art genomics platforms at the Vancouver Genome Sciences Centre and at the Centre for Molecular Medicine and Therapeutics. The project offers the chance to work at the forefront of connecting genome and phenotype in forest trees. Experience with some or all of the following would be advantageous: RNA purification, microarray technology, SNP detection assays, population genetics. Three Postdoctoral Fellow positions ? candidate gene discovery and analysis As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will be responsible for performing experiments to study Populus candidate genes that may be important in controlling secondary cell wall development and biochemistry. You will work with a bioinformatics team and use genome sequence information, expression profiling data, biological and mutant data, and phylogenetic analyses to identify candidate genes for further study in Populus. You will carry our a range of experiments in Populus including mis-expression in transgenic trees, expression profiling, and study of recombinant proteins to define the biological roles of these genes in secondary wall development. Experience in some or all of the following would be advantageous: molecular biology and genetics, biochemistry, RNA isolation and qRT-PCR, plant transformation, microarray technology. Postdoctoral Fellow position ? metabolic profiling and secondary cell wall biochemistry As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will be responsible for carrying out biochemical and metabolic analyses of the wood and secondary cell walls from natural and genetically modified Populus variants. This work will be carried out will the help and collaboration of team members working on the molecular biology of secondary wall formation in Populus, and international collaborators. Work will be carried out using state-of-the art analytical facilities in the Department of Wood Science at UBC. Familiarity and expertise in analytical biochemistry is required, with expertise in wood chemistry and metabolic profiling advantageous Postdoctoral Fellow position ? adaptive and biomass trait phenotyping As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will be responsible for obtaining phenotypic data on physiological traits related to adaptation and biomass productivity of trees from natural populations, as expressed in common garden and controlled environment experiments. These data will be analyzed with respect to the provenance of the genotypes (latitude, rainfall, elevation) and, in collaboration with other team members, related to population structure and tested for associations with SNP genotypes of candidate genes. Experience with some or all of the following would be advantageous: measurement of A/Ci curves by infra-red gas analysis, chlorophyll fluorescence, stable carbon and/or nitrogen isotope discrimination, hydroponics, water relations and frost hardiness assessment. Postdoctoral Fellow ? Wood rot fungi expression profiling As part of a team investigating the variation in natural populations of the completely sequenced model tree Populus trichocarpa (with particular reference to cellulosic biofuel traits), you will be responsible for carrying out studies on gene expression of wood rot fungi with completely sequenced genomes using microarray technology. This work will be carried out collaboratively with partners in the US, with the objective of understanding the nature of cell wall degrading systems deployed by these fungi, and the discovery of potential novel enzymes and enzyme complexes that could be deployed to efficiently de-construct Populus feedstocks of varying wood chemistry prior to fermentation of released sugars. Experience in some or all of the following would be advantageous: molecular biology, RNA isolation and qRT-PCR, transformation, microarray technology, fungal biology. --------------- Carl J. Douglas Professor University of British Columbia Department of Botany 6270 University Blvd Vancouver BC V6T 1Z4 Canada Tel: 1-604-822-2618 Fax: 1-604-822-6089 email: cdouglas@interchange.ubc.ca From schen from ufl.edu Sun Jul 6 11:23:35 2008 From: schen from ufl.edu (Sixue Chen) Date: Mon Jul 7 12:21:30 2008 Subject: [Arabidopsis] Postdoctoral Scholar - Functional Genomics of Guard Cell ABA Signaling Message-ID: <4870F187.3040206@ufl.edu> Two postdoctoral positions are open to study the redox and metabolomic regulatory networks underlying guard cell ABA signal transduction in model plant Arabidopsis thaliana. This project is based on collaborative efforts between Dr. Sally Assmann’s laboratory at Pennsylvania State University (http://www.bio.psu.edu/People/Faculty/Assmann/) and Dr. Sixue Chen’s laboratory at University of Florida (http://web.botany.ufl.edu/people/index.html). The two laboratories will work jointly on the following key aspects of ABA signal transduction in guard cells: a) ABA-responsive redox proteins and their functions; b) dynamics of guard cell metabolites in response to ABA; and c) systems biology models of guard cell ABA signal transduction. The postdoctoral associates will be responsible for 2D gel- and/or liquid chromatography-based redox proteomics, functional characterization of candidate redox-regulated proteins, metabolite profiling/metabolomics, and bioinformatics of guard cell redox proteins and metabolites. The associates are expected to receive multidisciplinary training, work in both laboratories if necessary, and be involved in the outreach activities of the project, such as organizing the summer proteomics workshop at University of Florida. Applicants are expected to have a strong publication record and demonstrated expertise in one or more of these areas: gel- and/or liquid chromatography-based proteomics, plant molecular biology, functional genomics, metabolite profiling and metabolomics, and/or plant systems biology. Experience with protein or metabolite separation/purification and mass spectrometry would be an advantage. Applicants should send a cover letter, a statement of research interests and a CV including the names and contact information of three references to: Dr. Sally Assmann, Department of Biology, Penn State University, 208 Mueller Lab., University Park, PA 16802, Email: sma3@psu.edu, or Dr. Sixue Chen, Department of Botany, UF Genetics Institute, Program of Plant Cell and Molecular Biology, University of Florida, PO Box 118526, Gainesville, FL 32610, Email: schen@ufl.edu. Applications will remain open until filled. Women and minorities are encouraged to apply. We encourage applications from individuals of diverse backgrounds. -- Sixue Chen Department of Botany, UF Genetics Institute, and Plant Molecular & Cellular Biology Program University of Florida Cancer & Genetics Research Complex, Room 438 1376 Mowry Road, PO Box 103610 Gainesville, FL 32610 USA Tel: +1 (352) 273-8330 Fax: +1 (352) 273-8284 Email: schen@ufl.edu Web page: http://www.botany.ufl.edu/people/index.html http://pmcb.ifas.ufl.edu/faculty.htm http://www.ufgi.ufl.edu/ From gzolla from yahoo.com Fri Jul 4 09:54:33 2008 From: gzolla from yahoo.com (Gaston Zolla) Date: Mon Jul 7 12:21:36 2008 Subject: [Arabidopsis] Hygromycin selection Message-ID: <79378.22604.qm@web31708.mail.mud.yahoo.com> Hi ? I was wondering if anyone has a protocol (or heard of one) for screening for hygromycin resistance by spraying the plants with hygromycin. I'd appreciate any information that people could offer. ? thanks in advance ? Gaston From gzolla from yahoo.com Fri Jul 4 09:09:23 2008 From: gzolla from yahoo.com (Gaston Zolla) Date: Mon Jul 7 12:21:40 2008 Subject: [Arabidopsis] ammoniun-free/nitrate-free MS medium Message-ID: <673965.88820.qm@web31708.mail.mud.yahoo.com> Hi Does?somebody know who is selling ammoniun-free/nitrate-free MS medium? it was sold by Sigma (cat no ?M9911)?or Lifetechnologies (cat no. 97-5068) and they are not more available!!!!!! Thanks in advance Gaston From rpj3 from leicester.ac.uk Mon Jul 7 12:14:27 2008 From: rpj3 from leicester.ac.uk (Jarvis, Dr R.P.) Date: Mon Jul 7 12:21:45 2008 Subject: [Arabidopsis] Postdoc position on chloroplast protein import, Leicester, UK Message-ID: <7722595275A4DD4FA225B92CDBF174A172A6819BBD@EXC-MBX3.cfs.le.ac.uk> Postdoctoral Research Associate Department of Biology University of Leicester, UK Salary Grade 7 - GBP 29,138 to GBP 33,780 per annum Available immediately for 36 months Ref: R3818 A BBSRC-funded postdoctoral position is available immediately for a strongly motivated individual to study the mechanism of chloroplast protein import in the model plant, Arabidopsis thaliana. Receptor components of the import machinery have multiple isoforms, and previous work indicated that these different isoforms define distinct, client-specific import pathways. This project aims to define these different pathways in greater detail. Experience of molecular biology research will be essential, and experience of research on Arabidopsis and/or organelle biology will be highly desirable. Informal enquiries are welcome and should be made to Dr Paul Jarvis (e-mail: rpj3@le.ac.uk or telephone: +44 116 223 1296). Downloadable application forms and further particulars are available from www.le.ac.uk/personnel/jobs. If you require a hard copy, please contact Personnel Services - tel: +44 116 252 2435, fax: +44 116 252 5140, email: recruitment4@le.ac.uk. Please note that CVs will only be accepted in support of a fully completed application form. Closing Date: 29 July 2008 Promoting equality of opportunity throughout the University ----------------------------------------------------------- Dr R. Paul Jarvis, Group Leader, Royal Society Rosenheim Research Fellow, Biology Department, University of Leicester, Leicester LE1 7RH, United Kingdom. ----------------------------------------------------------- Tel: +44-116-223-1296; Fax: +44-116-252-3330 E-mail: rpj3@le.ac.uk Web: ----------------------------------------------------------- From anirbanb from ncbs.res.in Tue Jul 8 07:54:15 2008 From: anirbanb from ncbs.res.in (Anirban Baral) Date: Tue Jul 8 11:53:05 2008 Subject: [Arabidopsis] Agrobacterium C 58 growth media Message-ID: <36381.192.168.1.1.1215521655.squirrel@mail.ncbs.res.in> Hi Arabineters, Can you please tell me if i need to add any antibiotic while growing untransformed Agrobacterium C58 in L.B ? Thanks in advance. Anirban -- Anirban Baral JUNIOR SCHOLAR NATIONAL CENTRE FOR BIOLOGICAL SCIENCES TATA INSTITUTE OF FUNDAMENTAL RESEARCH UAS-GKVK CAMPUS,BELLARY ROAD BANGALORE-560065,INDIA From phmasson from wisc.edu Wed Jul 9 15:07:28 2008 From: phmasson from wisc.edu (Patrick H Masson) Date: Wed Jul 9 15:09:28 2008 Subject: [Arabidopsis] Postdoc position available at the DOE Great Lakes Bioenergy Research Center Message-ID: <9EF74949-DAC8-4C6C-847F-6E241073BD91@wisc.edu> The new DOE Great Lakes Bioenergy Research Center (GLBRC) has one opening for postdoctoral research in a collaborative project on the identification and characterization of Arabidopsis genes and proteins involved in secondary cell wall biosynthesis and deposition. The position is immediately available and is located at the University of Wisconsin-Madison. Candidates should have a Ph.D. and a strong record of publication in peer-reviewed journals. They should have experience with bioinformatics, molecular genetics, and plant cell imaging (microscopy), and should be willing to collaborate with 3 other postdoctoral fellows working on distinct aspects of the same initiative. Applications should be submitted as single pdf files to postdoc@glbrc.wisc.edu . They should specify the position that they seek (position #20111.300), and include a statement of interest, vitae, names and contact information for 3 professional references. For further information, please contact Drs. Marisa Otegui (otegui@wisc.edu), Patrick H. Masson (phmasson@wisc.edu), David Rancour in the Bednarek laboratory (drancour@wisc.edu) or John Sedbrook (jcsedbr@ilstu.edu). Application review will begin immediately and continue until the position is filled. The University of Wisconsin-Madison is an Affirmative Action / Equal Opportunity Employer. From galbraith from arizona.edu Thu Jul 10 16:38:03 2008 From: galbraith from arizona.edu (David Galbraith) Date: Thu Jul 10 23:56:21 2008 Subject: [Arabidopsis] Microarray Workshop in Tucson, Arizona Message-ID: <6.2.3.4.2.20080710143323.082b20f0@ag.arizona.edu> NINTH INTERNATIONAL LONG-OLIGONUCLEOTIDE MICROARRAY WORKSHOP January 4-9, 2009 The University of Arizona Tucson, Arizona This workshop will comprise a combination of lectures and hand-on laboratory sessions. The participants will primarily employ Arabidopsis and maize (plant side) and human, bovine and porcine (animal side) whole genome 70-mer oligonucleotide microarrays in their laboratory work (for details of the plant arrays see http://www.ag.arizona.edu/microarray and http://www.maizearray.org/. The workshop will be divided into two parts: Part I (Sunday 4PM to Wednesday 5PM) will cover wet-lab aspects of microarray target production and amplification, microarray hybridization, and scanning. Part II (all day, Thursday and Friday) will concentrate on data extraction, statistical analysis, and experimental design. Together these topics are aimed at the goal of the participants obtaining optimal results using oligonucleotide-based microarrays. Part II may be taken separately. Specific topics to be covered include: * Experimental design. * Probe preparation and microarray printing. * Microarray rehydration and probe immobilization. * Target preparation, including RNA extraction, direct and indirect labeling, and amplification techniques. * Microarray hybridization. * Array scanning and data extraction. * Data analysis and archiving. Registration (Part I plus Part II) is $700, which includes costs of the microarrays and other supplies that you will use. Part II registration only is $300. Part I participants will be limited to 40 on a first-come, first-serve basis. Overall participation will be limited to 60 individuals. Note: There are a number of airline connections from Tucson to San Diego on Friday evening, allowing workshop participants convenient access to the Plant and Animal Genome XVII Meeting (January 10-14). For further details and to register, please contact David Galbraith (galbraith@arizona.edu) David W. Galbraith Professor of Plant Sciences & Professor, Bio5 Institute University of Arizona Office: 341 Keating Building Mailing address: BIO5 Institute The University of Arizona 1657 E. Helen St. Tucson, AZ 85721-0240 Tel: (520) 621-9153 Fax: (520) 626-4824 Email: galbraith@arizona.edu http://cals.arizona.edu/galbraith From aloraine from uncc.edu Fri Jul 11 10:03:06 2008 From: aloraine from uncc.edu (Loraine, Ann) Date: Fri Jul 11 12:11:48 2008 Subject: [Arabidopsis] postdoc position: bioinformatics & bench research, UNC Charlotte Message-ID: Postdoctoral researcher position in the Loraine lab at UNC Charlotte: A postdoctoral trainee position is available starting September, 2008 in the combined computational/experimental biology lab of Ann Loraine in the Bioinformatics Research Center at UNC Charlotte. The position will primarily involve bench research but will also have a substantial computational component. This position will be an excellent opportunity for a Ph.D. level scientist to gain expertise in bioinformatics while building an independent research program. For information about on-going projects in the lab, visit http://www.transvar.org. Candidate Profile: The ideal candidate should possess outstanding laboratory skills, including demonstrated ability to trouble-shoot and/or optimize lab protocols. Candidates should have extensive experience in Arabidopsis genetics, e.g., working with T-DNA insertion lines, growing plants, and performing crosses. He or she should have some experience applying bioinformatics techniques in research or classroom settings, such as simple scripting to automate repetitive tasks or using tools like R and Bioconductor to analyze data. Ability to learn and willingness to use software tools for managing research are absolutely essential. However, PhD-level expertise in computer science or advanced programming expertise is neither expected nor required. (We can teach you what you need to know.) The position is intended for PhD-level biologists who want to develop an independent, lab-based research program while at the same time gaining credentials in bioinformatics. Duties: Plan, perform, and trouble-shoot laboratory experiments; Present research at scientific conferences; Provide text and data for funding applications; Write scientific articles; Assist in setting up wet lab scheduled to open in October, 2008 at the North Carolina Research Campus; Assist in interviewing and training staff research technician for the lab; Help develop requirements documents for software development projects; Perform analysis of expression microarray data and other genome-scale data sets using custom tools developed by the PI and collaborators. Position Location: The position will be located at the North Carolina Research Campus, located around 20 minutes drive north of the UNC Charlotte main campus. Health insurance coverage is provided, with additional spousal or dependent coverage available for purchase. The applicant will join a vibrant and growing Bioinformatics program at UNCC, which currently includes ten faculty specializing in bioinformatics and computational biology. To apply: Send an e-mail to aloraine@uncc.edu with the following information: (1) introductory letter describing your interests and qualifications for the position, (2) a CV, (3) the names and contact information for 3 references, including at least one co-worker (e.g., fellow graduate student) from your thesis lab. From sascha.laubinger from tuebingen.mpg.de Fri Jul 11 03:37:53 2008 From: sascha.laubinger from tuebingen.mpg.de (Sascha Laubinger) Date: Fri Jul 11 12:12:35 2008 Subject: [Arabidopsis] At-TAX expression atlas Message-ID: Dear colleagues, We have set up a small Arabidopsis expression atlas (At-TAX) that is based on whole genome tiling arrays (http://www.weigelworld.org/resources/microarray/at-tax ). This allows for the first time expression analysis of more than 9,000 genes that cannot be analyzed with conventional arrays such as ATH1. The online tool for expression analysis is available trough http://jsp.weigelworld.org/tileviz/tileviz.jsp . We also identified more than a thousand novel transcribed regions in the genome that are visualized together with single probe raw intensities in all analyzed samples http://gbrowse.weigelworld.org/cgi-bin/gbrowse/attax/ . Additional information about the comparison of ATH1 and tiling arrays for expression analysis, non-polyadenylated transcripts in Arabidopsis etc., can be found in Sascha Laubinger, Georg Zeller, Stefan R. Henz, Timo Sachsenberg, Christian K. Widmer, Na?ra Naouar, Marnik Vuylsteke, Bernhard Sch?lkopf, Gunnar R?tsch and Detlef Weigel (2008) At-TAX: a whole genome tiling array resource for developmental expression analysis and transcript identification in Arabidopsis thaliana Genome Biology, 9:R112 Best wishes, Sascha --- Sascha Laubinger Max Planck Institute for Developmental Biology Spemannstrasse 37-39 D-72076 T?bingen Germany Tel: +49-7071-601 1406 Fax: +49-7071-601 1412 sascha.laubinger@tuebingen.mpg.de From vivian.irish from yale.edu Fri Jul 11 10:02:30 2008 From: vivian.irish from yale.edu (Vivian Irish) Date: Fri Jul 11 12:12:52 2008 Subject: [Arabidopsis] Postdoctoral positions -- transcriptional regulation of flower development Message-ID: Two full time postdoctoral associate positions are available in the laboratory of Dr. Vivian Irish, Dept. of Molecular, Cellular and Developmental Biology, Yale University. Arabidopsis petal development: The successful candidate will employ a variety of molecular genetic techniques and modeling approaches to elucidate the dynamics of transcriptional networks controlling petal organogenesis. Applicants should have experience in Arabidopsis molecular genetics, QTL analyses, and/or systems biology. Comparative analyses of flower and fruit development: The successful candidate will examine the changing roles of key transcription factors in regulating flower and fruit formation. This project will focus on utilizing genetically tractable members of the Solanaceae, and tomato in particular. Applicants should have experience in molecular genetics and phylogenetic analyses. For either position, a Ph.D. degree in molecular genetics, plant biology, or related field is required. Interested applicants should send a C.V., list of publications, brief summary of research experience and future goals, and names and addresses of three referees by email to Vivian Irish (Vivian.irish@yale.edu). Review of applications will begin immediately and continue until positions are filled. For further information on the lab interests, please see http://www.mcdb.yale.edu/irishlab/. For information on Yale University and New Haven please see http://www.yale.edu/. -- ************************** Vivian Irish Professor Department of Molecular, Cellular and Developmental Biology Yale University 266 Whitney Ave. New Haven, CT 06520-8104 phone 203-432-5572 FAX 203-432-5711 email vivian.irish@yale.edu http://www.mcdb.yale.edu/irishlab/ From jiangyqcn from hotmail.com Mon Jul 14 13:16:00 2008 From: jiangyqcn from hotmail.com (Jxy) Date: Mon Jul 14 13:34:07 2008 Subject: [Arabidopsis] primary and secondary At cell wall tissue Message-ID: Hi, colleagues, I want to know how to get the primary and secondary cell wall tissue from at plants less than one month old? Should I grow At plants to flowering before harvesting the secondary cell wall tissue such as stem? Thanks for your reply. Jiang _________________________________________________________________ If you like crossword puzzles, then you'll love Flexicon, a game which combines four overlapping crossword puzzles into one! http://g.msn.ca/ca55/208 From dsomers from crops.org Mon Jul 14 20:40:26 2008 From: dsomers from crops.org (David Somers) Date: Tue Jul 15 11:52:04 2008 Subject: [Arabidopsis] The Plant Genome - a new international online journal Message-ID: <1E763C55E318CD4CB6AD118FAE00ADB122630FDB@mail.agronomy.org> Please post this announcement. Dear Plant Genomics Colleague: The Plant Genome is an international open-access electronic journal published by the Crop Science Society of America. The goal of The Plant Genome is to provide the readership with a short submission-to-online publication of the latest advances and breakthroughs in plant genomics research. The Plant Genome publishes original research investigating all aspects of plant genomics. Technical breakthroughs reporting improvements in the efficiency and speed of acquiring and interpreting plant genomics data are welcome. The editorial board will give preference to novel reports that use innovative genomic applications that advance our understanding of plant biology and that may have applications to crop improvement. The journal also publishes invited review articles and perspectives that offer insight and commentary on recent advances in genomics and their potential for agronomic improvement. The first edition of the journal will be published on 23 July 2008. For more information on The Plant Genome, including instructions to authors and the editorial board, please go to The Plant Genome. Papers may be submitted through The Plant Genome's online submission website at: http://mc.manuscriptcentral.com/plantgenome. We look forward to receiving your manuscripts for publication in The Plant Genome. Sincerely, David A. Somers Editor From pspringer from ucr.edu Mon Jul 14 18:55:26 2008 From: pspringer from ucr.edu (Patricia Springer) Date: Tue Jul 15 11:52:44 2008 Subject: [Arabidopsis] Postdoc advertisement Message-ID: Please post the following job ad: thanks! A full time postdoctoral associate position is available immediately in the laboratory of Patricia Springer, Department of Botany and Plant Sciences, University of California, Riverside. The successful candidate will examine the role of individual members of the LOB-Domain (LBD) family of transcription factors in Arabidopsis development. This family of transcription factors has been implicated in a number of different developmental processes in plants, including the control of organ boundaries, adaxial-abaxial organ polarity, lateral root initiation, gametophyte development, and inflorescence branching. We seek to understand the functions of additional genes in this family. For more information about the available projects, contact Patricia Springer (pspringer@ucr.edu). A Ph.D. degree in molecular genetics, plant biology, or a related field is required. Candidates must have excellent communication skills. Interested applicants should send a C.V., list of publications, brief summary of research experience and future goals, and names and addresses of three referees by email to Patricia Springer (pspringer@ucr.edu). Review of applications will begin immediately and continue until position is filled. For further information on the lab interests, please see http://cepceb.ucr.edu/members/springer.htm. For information on the department and UCR, see http://plantbiology.ucr.edu/. -- Patricia Springer Associate Professor of Genetics Department of Botany and Plant Sciences Center for Plant Cell Biology University of California Riverside, CA 92521 patricia.springer@ucr.edu phone: 951-827-5785 fax: 951-827-4437 http://cepceb.ucr.edu From 2007203013 from njau.edu.cn Wed Jul 16 19:16:13 2008 From: 2007203013 from njau.edu.cn (2007203013@njau.edu.cn) Date: Wed Jul 16 23:32:10 2008 Subject: [Arabidopsis] about aux7 Message-ID: <3e9ea3cb-a884-44a6-93b1-164feca6c8fa@m73g2000hsh.googlegroups.com> hello anybody, i want to find a mutant which is insensitive to the auxin(IAA or GA3),so i find the type called aux7,i want to know the detail message about it ? thanks very much! From rpalanivelu from hotmail.com Thu Jul 17 02:41:35 2008 From: rpalanivelu from hotmail.com (Ravi Palanivelu) Date: Thu Jul 17 11:49:14 2008 Subject: [Arabidopsis] Reproductive biology conference in Tucson, Arizona, USA (October 17-19, 2008) Message-ID: <92f7c347-f3dc-4173-9312-cb73968df498@25g2000hsx.googlegroups.com> Dear colleagues, We invite you to participate in an upcoming conference in the field of plant reproductive biology, "Frontiers in Sexual Plant Reproduction III" (http://www.cals.arizona.edu/FSPRIII), that will be held in Tucson, Arizona on October 17-19, 2008 in the Tucson Marriott University Park. Early bird registration closes on July 31, 2008. Please register on or before this date to take advantage of low fees. The registration fees for the three-day meeting includes food {Breakfast, 10/17 - 10/19; lunch (10/17- 10/19) & dinner (10/17)}, transportation and visit to desert museum on 10/18 and banquet dinner in desert museum on 10/18. Participants are encouraged to submit an abstract and present a poster. Three faculty judges will identify top three posters for a cash award of $500 (first prize), $300 (second prize) and $200 (third prize). The prizes will be announced and awarded during the conference banquet dinner (October 18th, 2008). Six major sessions have been planned for the conference: Male gametophyte development and function, Female gametophyte development and function, Endosperm development and imprinting, Compatible and incompatible pollen-pistil interactions, Evolution of plant reproduction, Systems biology of plant reproduction and emerging technologies Preliminary program and information on registration, abstracts, lodging and travel is posted at the FSPR III conference website: http://www.cals.arizona.edu/FSPRIII. We look forward to seeing you in Tucson in October 2008 for an exciting scientific meeting on plant reproduction. Sincerely, The organizers: Dr. Ravi Palanivelu, University of Arizona (rpalaniv@ag.arizona.edu) Dr. Dmitry Belostotsky, University of Missouri, Kansas City. From huangy from performanceplants.com Fri Jul 18 13:23:43 2008 From: huangy from performanceplants.com (Dr. Yafan Huang) Date: Fri Jul 18 18:01:41 2008 Subject: [Arabidopsis] Three Research Positions Opened in Performance Plants Message-ID: <000001c8e903$6b8a7970$1d01a8c0@pp.loc> Performance Plants, Inc. Permanent Research Scientists Three Positions Available Immediately Two molecular biologists and one plant physiologist positions are immediately available in the Gene Discovery Program at the Company's Kingston research facility. The candidates for the first molecular position should have demonstrated laboratory skills and experience in plant tissue culture and transgenic regeneration. The candidates for the second molecular position should possess sound and broad molecular and biochemical laboratory skills. The applicants for the plant physiologist position should have specialized research background working in the field of plant phenotypic and physiological analysis. Applicants for these positions should possess a B.Sc. or M.Sc. degree, and have a strong background in plant biology. Extensive experience and knowledge on plant anatomy and development will be a plus. The candidates will ideally be detail-orientated, well-organized, enthusiastic and able to appreciate goal and team-based research and development. Performance Plants Inc. is a Canadian plant biotechnology company, whose mission is to discover, develop and validate innovative plant technologies that optimize crop performance and agricultural productivity while improving food & fuel security, preserving the environment and promoting global sustainability. The Company's head office is located in the beautiful city of Kingston by Lake Ontario. Interested applicants should forward their curriculum vitae (including the names of three references) to: Human Resources, Performance Plants Inc. 700 Gardiners Road Kingston, Ontario K7M 3X9, Canada Email: humanresources@performanceplants.com. Fax: 1-(613)-545-3618. Performance Plants Inc. offers a complete benefits package and is an equal opportunity employer. Only applicants considered for an interview will receive further communication after their initial application. From jdfriesner from ucdavis.edu Sat Jul 19 09:40:27 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Sat Jul 19 13:08:01 2008 Subject: [Arabidopsis] Arabidopsis conference abstracts and program book available online Message-ID: <200807191440.m6JEeRrm013592@cucujus.ucdavis.edu> Abstracts and conference book for the 19th International Conference on Arabidopsis Research are available online: at conference website: abstracts: http://www.plantconferences.org/Arabidopsis2008/abstracts/ program: http://www.plantconferences.org/Arabidopsis2008/Arabidopsis08_Program.pdf at TAIR: http://www.arabidopsis.org/news/abstracts.jsp From shahzadbio from yahoo.com Sat Jul 19 11:31:09 2008 From: shahzadbio from yahoo.com (shahzad anjam) Date: Sun Jul 20 16:49:30 2008 Subject: [Arabidopsis] Re: Arab-gen Digest, Vol 39, Issue 8 Message-ID: <330267.23732.qm@web36302.mail.mud.yahoo.com> Dear Cooleagues, I have transgenic wheat plants in hand, varified by?PCR. But I am disappointed?that when I try its southren from genomic DNA, it never produce results. The southren of the PCR product of the same plants is positive but from genomic DNA, it is not. I?am using Non-redioactive Chromogenic Detection?kit (Fermentas) for southren. I restrict the genomic DNA with HindIII enzyme. I have also used almost 20 ug DNA in southren. I am in trouble in this case. I have also performed the physiological assays of plants, and they are showing significantly different results from controll plants, (indicating the presence of gene presence). Without doing southren I am not able to publish the rsults. If someone can help me plz. contact met at shahzadbio@yahoo.com thanks ? ? ? ? Muhammad Shahzad Anjam? Biotechnology, Institute of Biotechonology ,? Bahauddin Zakariya University , Multan (www.bzu.edu.pk) Pakistan . Office (Phone) +92(0)61 9210463, +92(0)61 9210071-4, Ext: 3900-3904 Cell Phone: +92-333-8174001 E-mail: shahzadbio@yahoo.com ----- Original Message ---- From: "arab-gen-request@oat.bio.indiana.edu" To: arab-gen@magpie.bio.indiana.edu Sent: Thursday, July 17, 2008 22:03:38 Subject: Arab-gen Digest, Vol 39, Issue 8 Send Arab-gen mailing list submissions to ??? arab-gen@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit ??? http://www.bio.net/biomail/listinfo/arab-gen or, via email, send a message with subject or body 'help' to ??? arab-gen-request@net.bio.net You can reach the person managing the list at ??? arab-gen-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Arab-gen digest..." Today's Topics: ? 1. about aux7 (2007203013@njau.edu.cn) ? 2. Reproductive biology conference in Tucson, Arizona,??? USA ? ? ? (October 17-19, 2008) (Ravi Palanivelu) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Jul 2008 17:16:13 -0700 (PDT) From: 2007203013@njau.edu.cn Subject: [Arabidopsis] about aux7 To: bionet-genome-arabidopsis@moderators.isc.org Message-ID: ??? <3e9ea3cb-a884-44a6-93b1-164feca6c8fa@m73g2000hsh.googlegroups.com> Content-Type: text/plain; charset=ISO-8859-1 hello? anybody, i want to find a mutant which is insensitive to the auxin(IAA or GA3),so i find the type called aux7,i want to know the detail message about it ? thanks very much! ------------------------------ Message: 2 Date: Thu, 17 Jul 2008 00:41:35 -0700 (PDT) From: Ravi Palanivelu Subject: [Arabidopsis] Reproductive biology conference in Tucson, ??? Arizona,??? USA (October 17-19, 2008) To: bionet-genome-arabidopsis@net.bio.net Message-ID: ??? <92f7c347-f3dc-4173-9312-cb73968df498@25g2000hsx.googlegroups.com> Content-Type: text/plain; charset=ISO-8859-1 Dear colleagues, We invite you to participate in an upcoming conference in the field of? plant reproductive biology, "Frontiers in Sexual Plant Reproduction III" (http://www.cals.arizona.edu/FSPRIII), that will be held in Tucson, Arizona on October 17-19, 2008 in the Tucson Marriott University Park. Early bird registration closes on July 31, 2008. Please register on or before this date to take advantage of low fees. The registration fees for the three-day meeting includes food {Breakfast, 10/17 - 10/19; lunch (10/17- 10/19) & dinner (10/17)}, transportation and visit to desert museum on 10/18 and banquet dinner in desert museum on 10/18. Participants are encouraged to submit an abstract and present a poster. Three faculty judges will identify top three posters for a cash award of $500 (first prize), $300 (second prize) and $200 (third prize). The prizes will be announced and awarded during the conference banquet dinner (October 18th, 2008). Six major sessions have been planned for the conference: Male gametophyte development and function, Female gametophyte development and function, Endosperm development and imprinting, Compatible and incompatible pollen-pistil interactions, Evolution of plant reproduction, Systems biology of plant reproduction and emerging technologies Preliminary program and information on registration, abstracts, lodging and travel is posted at the FSPR III conference website: http://www.cals.arizona.edu/FSPRIII. We look forward to seeing you in Tucson in October 2008 for an exciting scientific meeting on plant reproduction. Sincerely, The organizers: Dr. Ravi Palanivelu, University of Arizona (rpalaniv@ag.arizona.edu) Dr. Dmitry Belostotsky, University of Missouri, Kansas City. ------------------------------ _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen End of Arab-gen Digest, Vol 39, Issue 8 *************************************** Send instant messages to your online friends http://uk.messenger.yahoo.com From jerome.bove from umontreal.ca Mon Jul 21 12:26:14 2008 From: jerome.bove from umontreal.ca (Jerome Bove) Date: Mon Jul 21 15:52:20 2008 Subject: [Arabidopsis] Postdoc position Message-ID: - Dynamics and spatial organization of vesicle trafficking and secretion in plant cell morphogenesis -* *The University of Montreal is among the top 100 universities worldwide. A section of the Department of biological sciences is located in the Montreal Botanical Garden at the Plant Biology Research Institute (Institut de recherche en biologie v=E9g=E9tale, IRBV). The Laboratory of plant cell bio= logy and cytomechanics is interested in the architecture and mechanics of plant cell structure and the dynamics of plant cell morphogenesis. We use microscopy, micro-manipulation and mechanical modeling to understand plant cell functioning and the mechanics of cellular growth processes. Plant cell morphogenesis requires the targeted transport of cell wall polymers and secreted proteins to precisly defined regions on the cell surface. In growing pollen tubes, this involves highly efficient vesicle trafficking to the extreme, growing end of the cell which elongates at rate= s of several micrometers per minute. In the present project, the subcellular localization of proteins involved in the targeting of these secretory vesicles will be studied using transformation with GFP (green fluorescent protein). This project is financed by the Human Frontier Science Program. The following article reports recent data related to the present project: Bove J, Vaillancourt B, Kroeger J, Hepler PK, Wiseman PW, Geitmann A (2008) Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching. Plant Physiology, www.plantphysiol.org/cgi/doi/10.1104/pp.108.120212 Other publications from the Geitmann lab can be found at the web site indicated below. Requirements =95 PhD in plant molecular biology or related area, received within the las= t five years. =95 Excellent academic record. =95 Excellent communication skills. =95 Very strong background in plant molecular biology (vector construction, Arabidopsis genetics and transformation): The candidate must be able to completely independently plan, organize, and carry out the molecular biolog= y aspects of the project as well as assist graduate students with these techniques. =95 Experience with fluorescent protein detection and confocal laser scanni= ng microscopy. =95 Experience in transmission electron microscopy is an asset. =95 Knowledge of the French language is not mandatory but represents an ass= et. Application Please send a detailed CV containing academic transcripts, a brief outline of experience, a list of publications, and the names of three references to the e-mail address below. The successful candidate will start in September 2008 or soon after. The initial contract will have a duration of one year. Extension may be possible depending on the availability of research funds. Contact Dr. Anja Geitmann Institut de recherche en biologie v=E9g=E9tale D=E9partement de sciences biologiques, Universit=E9 de Montr=E9al 4101, rue Sherbrooke est, Montr=E9al, Qu=E9bec H1X 2B2, Canada anja.geitmann@umontreal.ca; phone 514-872 8492 www.irbv.umontreal.ca/geitmann.htm From nicholas.provart from utoronto.ca Tue Jul 22 13:19:55 2008 From: nicholas.provart from utoronto.ca (Nicholas Provart) Date: Tue Jul 22 14:53:28 2008 Subject: [Arabidopsis] Cross-species expression viewing at the BAR: Poplar eFP Browser Message-ID: <200807221820.m6MIKLO18472@net.bio.net> Hello everyone, Just in time for the 19th ICAR: cross-species expression viewing at the Bio-Array Resource (BAR)! We have precomputed all orthologs between poplar and Arabidopsis using OrthoMCL. In the output eFP Browser page for a given Arabidopsis gene, if there are poplar orthologs then links to a poplar developmental series eFP Browser are provided, to help with the identification of the corresponding poplar functional ortholog. We also provide links to the sequence alignments between a given Arabidopsis gene and its poplar ortholog(s). The cross-species feature and the Poplar eFP Browser are available at htpp://bar.utoronto.ca. The data for the Poplar eFP Browser come from Olivia Wilkins in the Campbell Laboratory at the University of Toronto, and will be the subject of a forthcoming publication. Thanks to Hardeep Nahal and Justin Foong for implementing the Poplar eFP Browser. Best regards and hope to see you in Montreal! Nick ......................................... Nicholas Provart, PhD Assistant Professor, Plant Bioinformatics & Systems Biology Director, Graduate Program in Genome Biology and Bioinformatics Member, Centre for the Analysis of Genome Evolution and Function Rm 3051, Dept. of Cell and Systems Biology, Uni. Toronto 25 Willcocks St., Toronto, ON. M5S 3B2. CANADA Tel. (+1) (416) 978-7141, Fax. (+1) (416) 978-5878 URL. http://www.csb.utoronto.ca/faculty/provart-nicholas Arabidopsis Tools. http://www.bar.utoronto.ca email. nicholas.provart@utoronto.ca TTC. Spadina LRT, Willcocks St. From ojahns from CeBiTec.Uni-Bielefeld.DE Thu Jul 24 04:05:44 2008 From: ojahns from CeBiTec.Uni-Bielefeld.DE (Oliver Jahns) Date: Thu Jul 24 11:41:14 2008 Subject: [Arabidopsis] Estland-1 (Est-1) seeds! Message-ID: <000301c8ed6c$7553dba0$5ffb92e0$@Uni-Bielefeld.DE> Hello everybody, does anyone have Estland-1 (Est-1) seeds? I really need some of those. Thanks, Oliver ------------------------------ Oliver Jahns Dept. of Genome Research University of Bielefeld D-33594 Bielefeld Germany Room: G0-133 Phone: +49-(0)521-106-8730 Fax: +49-(0)521-106-6423 ojahns@cebitec.uni-bielefeld.de From raymond.didonato from biobase-international.com Fri Jul 25 13:01:49 2008 From: raymond.didonato from biobase-international.com (Raymond DiDonato) Date: Fri Jul 25 14:11:04 2008 Subject: [Arabidopsis] BIOBASE Products for Plant Researchers Message-ID: <488A150D.2000803@biobase-international.com> For over ten years, BIOBASE has offered researchers high quality, manually curated databases of relevant scientific literature, including TRANSFAC? and YPD^(TM), which save researchers time by offering easy access to the essential data they need to perform their analyses. We also offer gene expression analysis tools and software. We thought you may find our latest plant focused products useful in your research. * The BIOBASE Knowledge Library^(TM): Plant Edition* BKL PLANT is a resource for researchers studying crop optimization, disease resistance, stress tolerance and other areas of plant research, and contains: . Protein information representing over 100 plant species, including Arabidopsis, rice, and maize . Manually curated ontology assignments to capture biological and molecular function, cellular localization, gene and protein expression, and phenotype details . In-depth gene regulation data, signaling / metabolic pathways, and more *ExPlain^(TM) PLANT* The ExPlain^(TM) Analysis Platform provides users the ability to generate hypotheses regarding the upstream cause(s) (Key Molecules such as kinases, adaptor proteins, receptors, etc.) of observed gene expression changes. . Access over 50,000 promoters combined from Arabidopsis and rice representing nearly 60 matrices . Link to detailed promoter binding site and matrix information . Use microarray, ChIP-chip, or proteomics data to build upstream and downstream pathways . Go beyond traditional microarray analysis to find non-differentially expressed factors influencing gene expression . Use BKL-Plant pathway data for upstream and downstream target analysis or supplement with your own interaction data To learn more about BKL-Plant and ExPlain-Plant, please visit our website at: http://www.biobase-international.com/pages/index.php?id=446 To register for a free trial of BKL-Plant or ExPlain-Plant, please visit us at: https://portal.biobase-international.com/cgi-bin/portal/addtrial.cgi From bd.gregory.salk from gmail.com Sat Jul 26 21:31:06 2008 From: bd.gregory.salk from gmail.com (Brian D. Gregory) Date: Sun Jul 27 23:34:16 2008 Subject: [Arabidopsis] Data added to smRNAome AnnoJ browser Message-ID: <788947B6-2916-481E-9F34-27AC8F440A26@salk.edu> To the Arabidopsis Community, We have added the deep sequencing data, which maps uncapped and =20 cleaved transcripts of Arabidopsis from: 1) Gregory, B.D., O=92Malley, R.C., Lister, R., Urich, M.A., Tonti-=20= Filippini, J., Chen, H., Millar, A.H., and Ecker, J.R. 2008. A link =20 between RNA metabolism and silencing affecting Arabidopsis =20 development. Dev. Cell 14: 854-866. 2) Addo-Quaye, C., Eshoo, T.W., Bartel, D.P., and Axtell, M.J. =20 2008. Endogenous siRNA and miRNA targets identified by sequencing of =20 the Arabidopsis degradome. Curr. Biol. 18: 758-762. 3) German, M.A., Pillay, M., Jeong, D.H., Hetawal, A., Luo, S., =20 Janardhanan, P., Kannan, V., Rymarquis, L.A., Nobuta, K., German, R., =20= De Paoli, E., Lu, C., Schroth, G., Meyers, B.C., Green, P.J. 2008. =20 Global identification of microRNA-target RNA pairs by parallel =20 analysis of RNA ends. Nat. Biotechnol. doi:10.1038/nbt1417. into our dynamic Web2.0 based genomic browser called AnnoJ, which is =20 designed to effectively handle both current and emerging genomic data-=20= types. The browser displays (from the megabase down to the single =20 base) these sequencing data sets. These data sets are all open access, and to view them in this browser =20= just go to the URL below with Firefox or Safari: http://neomorph.salk.edu/aj/pages/smRNAome.html Brian D. Gregory Damon Runyon Postdoctoral Fellow Plant Biology Laboratory (Ecker) The Salk Institute for Biological Studies 10010 North Torrey Pines Rd. LaJolla, CA 92037 Phone (858) 453-4100 x1533 =20= From altpeter from ufl.edu Mon Jul 28 16:02:30 2008 From: altpeter from ufl.edu (Altpeter,Fredy) Date: Mon Jul 28 18:42:14 2008 Subject: [Arabidopsis] Postdoctoral Research Associate Position in Breeding and Biotechnology of Biofuel Crops Message-ID: <18967E83B2946D4DBB46899C01D36A73367FE8AD39@UFEXCH-MBXCL01.ad.ufl.edu> Postdoctoral Research Associate Position in Breeding and Biotechnology of Biofuel Crops Position is available for postdoctoral research associate in the Laboratory of Plant Molecular Physiology, Agronomy Department, IFAS, University of Florida. Lignocellulosic materials produced by biomass grasses are a promising feedstock to produce low-cost ethanol. Our research program integrates traditional plant breeding, mutagenesis, advanced genetic transformation technology and functional genomics to identify, isolate and engineer limiting factors for improvement of grasses http://agronomy.ifas.ufl.edu/pdfs/Genes.pdf. Postdoctoral research will focus on both, the genetic improvement and the reduction of invasiveness, of the biofuel crop elephantgrass. Research will involve the comparison of traditional breeding, interspecific hybridization, chemical mutagenesis and transgenic approaches. This is an ideal opportunity for meaningful publications and future professional development in either academia or industry. Excellent communication skills and publications demonstrating expertise in a range of traditional breeding and molecular techniques are required for the postdoctoral position. Annual starting salary is $ 30,000 plus benefits. Interested applicants should send: Current resume including names, addresses, and telephone numbers of three references Applications and questions related to the application process should preferably be send by e-mail to altpeter@ufl.edu or addressed to: Dr. Fredy Altpeter Agronomy Department University of Florida, IFAS PO Box 11O300 Gainesville, FL 32611-300 Review of applications will start immediately and continue until a suitable candidate is identified. Gainesville, the home town of the University of Florida, is located half way between the Atlantic Ocean and the Gulf of Mexico with a population of 140 000 (46 000 students) and has frequently achieved top ratings in quality of life and affordability http://gainesville.com/apps/pbcs.dll/article?AID=/20070506/SUNFRONT/7.... More information on the University of Florida is available at http://www.ufl.edu University of Florida is an Affirmative Action/Equal Opportunity Employer (male/female) and does not discriminate on the basis of race, color, creed, religion, national origin, ancestry, citizenship, age, gender, sexual orientation, disability, or Vietnam Era Veteran status in the offering of all benefits, services, and educational and employment opportunities. Women and minorities are encouraged to apply. ********************************************************** Fredy Altpeter Associate Professor Laboratory of Molecular Plant Physiology Agronomy Department University of Florida, IFAS PO Box 11O300 2191 McCarty Hall Gainesville, FL32611-300 Tel.: 352 392 1823 ext. 204, 216 or 218 Fax: 352 392 7248 E-mail: altpeter@ufl.edu http://agronomy.ifas.ufl.edu/faculty/altpeter.shtml http://pmcb.ifas.ufl.edu/faculty.htm http://www.ufgi.ufl.edu/aspzzz/members1.asp http://www.energy.ufl.edu/index.php?src=faculty&ID=79 ********************************************************** From altpeter from UFL.EDU Mon Jul 28 16:11:11 2008 From: altpeter from UFL.EDU (Altpeter,Fredy) Date: Mon Jul 28 18:42:19 2008 Subject: [Arabidopsis] Two Graduate Research Assistantships (1 Ph.D, 1 MS) in Genetic Improvement of BIOENERGY Grasses Message-ID: <18967E83B2946D4DBB46899C01D36A73367FE8AD3A@UFEXCH-MBXCL01.ad.ufl.edu> Two Graduate Research Assistantships (1 Ph.D, 1 MS) in Genetic Improvement of BIOENERGY Grasses are available in the Laboratory of Plant Molecular Physiology, Agronomy Department, IFAS, Univ. of Florida http://agronomy.ifas.ufl.edu/Altpeter.HTML. Lignocellulosic materials produced by biomass grasses are a promising feedstock to produce low-cost ethanol. Genetic improvement of grasses through genetic engineering or targeted mutagenesis will enhance their quality and reduce costs associated with biomass conversion to fuel. A description of our research objectives to develop genetically enhanced grasses can be found at: http://www.mae.ufl.edu/Energy/index.php?src=faculty&ID=79. Efficient nuclear grass transformation or mutagenesis protocols are established in our laboratory. Job Description: Selected candidates will focus on RNAi suppression of rate limiting genes in the lignin biosynthetic pathway or high level expression of cell wall degrading enzymes to enhance biomass quality and quantity. This will include gene cloning, tissue culture, gene transfer, molecular and biochemical characterization of improved grasses. Qualifications: The successful applicants must have a first-class Master's or BS degree in Molecular Biology, Biotechnology, Biochemistry, Plant Science or Microbiology. The successful Ph.D candidates will have demonstrated experience in standard molecular biology techniques and plant transformation and a demonstrated ability to perform research at an advanced level. Superior communication skills and the ability to work both independently and in a team are essential. Stipend: Stipend is commensurate with training and experience and will include tuition and a contribution to health insurance. To Apply: You will find information on how to apply at http://agronomy.ifas.ufl.edu/gradadmission.html. Send also a cover letter describing your research interests and career goals, CV, transcripts, and the contact information for three references preferably by e-mail to faltpeter@ifas.ufl.edu or addressed to: Dr. Fredy Altpeter, Agronomy Department, University of Florida, IFAS, PO Box 11O300, Gainesville, FL 32611-300. Review of applications will start immediately and continue until suitable candidates are identified. Research may potentially start as early as September 2008 but applicants must apply for a January 2009 start date in the Ph.D. or Masters program. Gainesville, the home town of the University of Florida, is located half way between the Atlantic Ocean and the Gulf of Mexico with a population of 140 000 (46 000 students) and has frequently achieved top ratings in quality of life and affordability http://gainesville.com/apps/pbcs.dll/article?AID=/20070506/SUNFRONT/7.... More information on the University of Florida is available at http://www.ufl.edu. University of Florida is an Affirmative Action/Equal Opportunity Employer (male/female) and does not discriminate on the basis of race, color, creed, religion, national origin, ancestry, citizenship, age, gender, sexual orientation, disability, or Vietnam Era Veteran status in the offering of all benefits, services, and educational and employment opportunities. Women and minorities are encouraged to apply. ********************************************************** Fredy Altpeter Associate Professor Laboratory of Molecular Plant Physiology Agronomy Department University of Florida, IFAS PO Box 11O300 2191 McCarty Hall Gainesville, FL32611-300 Tel.: 352 392 1823 ext. 204, 216 or 218 Fax: 352 392 7248 E-mail: altpeter@ufl.edu http://agronomy.ifas.ufl.edu/faculty/altpeter.shtml http://pmcb.ifas.ufl.edu/faculty.htm http://www.ufgi.ufl.edu/aspzzz/members1.asp http://www.energy.ufl.edu/index.php?src=faculty&ID=79 ********************************************************** From clchiham from ucdavis.edu Tue Jul 29 12:09:54 2008 From: clchiham from ucdavis.edu (CECILIA CHI-HAM) Date: Tue Jul 29 14:06:30 2008 Subject: [Arabidopsis] FW: Please Post: PostDoctoral Position, Univ California Davis Message-ID: Please post the following postdoctoral position ad: Postdoctoral position to work on novel corn genotypes at the University of California, Davis. Research will involve metagenomic analysis of free-living and endophytic microbes associated with corn, analysis of aerial root mucigel composition and of nitrogen assimilation. Ph.D. with experience in molecular genetics and plant physiology required. Spanish language skills preferred. Contact Dr. Alan Bennett at abbennett@ucdavis.edu . From rpalanivelu from hotmail.com Tue Jul 29 13:25:19 2008 From: rpalanivelu from hotmail.com (Ravi Palanivelu) Date: Tue Jul 29 14:06:46 2008 Subject: [Arabidopsis] Early-bird registration reminder: Plant Reproductive biology conference in Tucson, AZ, USA Message-ID: <771c7f03-e6e9-4ea4-b01e-c4838fabdd63@n33g2000pri.googlegroups.com> Dear colleagues, We invite you and your lab to participate in an upcoming conference in the field of plant reproductive biology, "Frontiers in Sexual Plant Reproduction III" (http://www.ag.arizona.edu/FSPRIII), that will be held in Tucson, Arizona on October 17-19, 2008 in the Tucson Marriott University Park. Six major sessions have been planned for the conference: Male gametophyte development and function, Female gametophyte development and function, Endosperm development and imprinting, Compatible and incompatible pollen-pistil interactions, Evolution of plant reproduction, Systems biology of plant reproduction and emerging technologies Preliminary program and information on registration, abstracts, lodging and travel is posted at the FSPR III conference website: http://www.ag.arizona.edu/FSPRIII. **Early bird registration closes on July 31, 2008. Please register on or before this date to take advantage of low fees**. The registration fees for the three-day meeting includes food {Breakfast, 10/17- 10/19; lunch (10/17- 10/19) & dinner (10/17)}, transportation and visit to desert museum on 10/18 and banquet dinner in desert museum on 10/18. Participants are encouraged to submit an abstract and present a poster. Three faculty judges will identify top three posters for a cash award of $500 (first prize), $300 (second prize) and $200 (third prize). The prizes will be announced and awarded during the conference banquet dinner (October 18th, 2008). Please forward this to other colleagues who may be interested in this announcement. We look forward to seeing you in October 2008 for an exciting scientific meeting on plant reproduction. Sincerely, The organizers: Dr. Ravi Palanivelu, University of Arizona (rpalaniv@ag.arizona.edu) Dr. Dmitry Belostotsky, University of Missouri, Kansas City. From bouchez from versailles.inra.fr Thu Jul 31 03:15:18 2008 From: bouchez from versailles.inra.fr (David Bouchez) Date: Thu Jul 31 11:44:59 2008 Subject: [Arabidopsis] Post-doc positions at IJPB (Versailles, France) Message-ID: SEVEN POST-DOCTORAL POSITIONS AVAILABLE The Jean-Pierre Bourgin Institute, Versailles, France For candidates with outstanding academic record and excellent communication skills. Positions are located in Versailles (France) for 3 years, starting approx. January 2009. Salary is 2200-2500 euros per month. Further information and contact : http://www-ijpb.versailles.inra.fr/en/institut/offres.htm The Jean-Pierre Bourgin Institute hosts 25 research groups devoted to plant biology, totalizing 330 people, comprised of 230 permanent staff and 100 post-docs, PhD students and others students. The IJPB has a long-standing and respected expertise in the field of plant biology, including genomics, genetics, breeding, metabolism, cell biology and development. All tools and resources relevant for integrative plant biology are available on site. Local facilities in Versailles include outstanding experimental facilities dedicated to plant culture (4500 m2 glasshouses and 500 m2 controlled environment rooms), state of the art technical platforms for Microscopy and Cytology (4 confocal microscopes, classical microscopy, etc), Protein Biochemistry (electrophoresis, chromatography, MALDI-TOF MS…), Chemistry (FT-IR, several mass spec including GC-TOF and LC-MS/MS…), and Bioinformatics. David Bouchez Institut Jean-Pierre Bourgin Station de Génétique et Amélioration des Plantes INRA Centre de Versailles - 78026 Versailles Cedex France tel : +33 (0)1 30 83 33 94 - fax : +33 (0)1 30 83 33 19 e-mail : bouchez@versailles.inra.fr http://www-ijpb.versailles.inra.fr cytoskeleton group : http://www-ijpb.versailles.inra.fr/en/sgap/equipes/cyto/ From braam from rice.edu Thu Jul 31 15:38:55 2008 From: braam from rice.edu (Janet Braam) Date: Thu Jul 31 19:22:42 2008 Subject: [Arabidopsis] Plant Biology Lab Technician Position - Rice University Message-ID: <2C73D60B-ADD0-42E3-BF8A-AD928E3A511C@rice.edu> PLANT BIOLOGY LAB TECHNICIAN POSITION AVAILABLE PLANT STRESS RESPONSES: CALCIUM SIGNALING, REDOX AND NITRIC OXIDE REGULATION, AUTOPHAGY MECHANISMS, CHROMATIN MODIFICATION Laboratory of Janet Braam Biochemistry and Cell Biology Rice University A technician position is available to participate in research aimed to determine mechanisms by which plants sense environmental stresses, interpret calcium signals, and regulate cellular and organismal responses. Bachelor's degree in biological science is required with at least one year of research experience in plant biology, cell biology, biochemistry, fluorescence microscopy, and/or genetics. Strong communication skills and an ability and willingness to collaborate are essential. Applicants should send a curriculum vitae, a cover letter describing research experience and interests, and the names and email addresses for at least three references to: braam@rice.edu Janet Braam Chair and Professor Biochemistry and Cell Biology Rice University Houston, TX 77005-1892 http://bioc.rice.edu/~braam/ Applications will be reviewed until the position is filled. Rice University is an equal opportunity/affirmative action employer. From gzolla from yahoo.com Thu Jul 31 10:41:14 2008 From: gzolla from yahoo.com (Gaston Zolla) Date: Fri Aug 1 12:44:58 2008 Subject: [Arabidopsis] seeds of CH5B-Gus Message-ID: <663375.22502.qm@web31702.mail.mud.yahoo.com> Hi Does anybody has some seeds of?? CH5B-GUS, line L7, that was developed by Dr.Bleecker? Thanks in advance Gaston