From rpalanivelu from hotmail.com Sun Jun 1 23:11:54 2008 From: rpalanivelu from hotmail.com (Ravi Palanivelu) Date: Mon Jun 2 01:24:32 2008 Subject: [Arabidopsis] Deadlines for abstract submission and financial application submission to FSPRIII conference have been extended! Message-ID: <3bdc3817-c5b2-4302-bde2-6ff3f234ea8f@l28g2000prd.googlegroups.com> Dear colleagues, We would like to bring to your attention the following announcements: 1. The deadline for abstracts to be considered for an oral presentation in the upcoming Frontiers in Sexual Plant Reproduction III (FSPRIII), to be held in Tucson, Arizona on October 17-19, 2008 has been extended to June 16, 2008 (Monday). In each of the six sessions planned for the conference, two abstracts submitted by the deadline will be selected for oral presentations. Please access the following link to learn more about abstract submission: http://cals.arizona.edu/FSPRIII/abstracts.html 2. Deadline for financial assistance application submission has also been extended to June 16, 2008 (Monday). With generous support from USDA, we are pleased to announce that 10 awards (each = $500) will be available to graduate students and postdocotoral fellows. Women and participants from under represented groups will be given priority. To be considered for the award, please register, email your abstract and a brief justification demonstrating the need for support no later than June 16, 2008 to Ravi Palanivelu at rpalaniv@ag.arizona.edu. Please access the following link to learn more about obtaining financial assistance to attend FSPRIII: http://cals.arizona.edu/FSPRIII/assistance.html 3. We are pleased to announce that the secure online registration for the FSPRIII is now open. Please access the following link to register for this conference: http://cals.arizona.edu/FSPRIII/conference-registration.html Six major sessions have been planned for the conference: Pollen development and tube growth, Female gametophyte development and function, Endosperm development and imprinting, Compatible and incompatible pollen-pistil interactions Evolution of plant reproduction, Systems biology of plant reproduction and emerging technologies We invite you to participate in this meeting and share your latest results on reproductive biology research with your colleagues. We look forward to seeing you in Tucson in October 2008 for an exciting scientific meeting on plant reproduction. Sincerely, The organizers: Dr. Ravi Palanivelu, University of Arizona (rpalaniv@ag.arizona.edu) Dr. Dmitry Belostotsky, University of Missouri, Kansas City. From braam from rice.edu Mon Jun 2 08:45:31 2008 From: braam from rice.edu (Janet Braam) Date: Mon Jun 2 23:36:32 2008 Subject: [Arabidopsis] Postdoctoral Position Announcement Message-ID: POSTDOCTORAL POSITION AVAILABLE: PLANT STRESS RESPONSES: CALCIUM SIGNALING, REDOX AND NITRIC OXIDE REGULATION, AUTOPHAGY MECHANISMS Laboratory of Janet Braam Biochemistry and Cell Biology Rice University A postdoctoral position is available in research to determine the mechanisms by which plants sense environmental stresses, interpret calcium signals, and regulate cellular and organismal responses. A Ph.D. in biological science is required. A strong background in plant biology, cell biology, biochemistry, and/or genetics is desired. Research experience in the areas of autophagy, mitochondrial function, and/or nitric oxide regulation is advantageous. Excellent communication skills and an ability and willingness to collaborate are essential. Only highly motivated individuals with proven track records should apply. Applicants should send a curriculum vitae, a cover letter describing research experience and interests, and the names and email addresses for at least three references to: braam@rice.edu Janet Braam Chair and Professor Biochemistry and Cell Biology Rice University Houston, TX 77005-1892 http://bioc.rice.edu/~braam/ Applications will be reviewed until the position is filled. Rice University is an equal opportunity/affirmative action employer. From itot from tll.org.sg Mon Jun 2 04:07:43 2008 From: itot from tll.org.sg (Toshiro Ito) Date: Mon Jun 2 23:39:04 2008 Subject: [Arabidopsis] gateway binary vector for Norf selection Message-ID: Hello everyone, I am looking for a gateway destination vector (with attR1 and attR2) for Arabidopsis transformation of single DNA fragment, resistant to the herbicide norflurazon. If anyone is willing to share such a plasmid in the backbone of pZP, pCAMBIA or any binary vector with high transformation efficiency, I would be really grateful. Thank you, Toshiro -- -------------------------------------- Toshiro Ito itot@tll.org.sg Temasek Life Sciences Laboratory 1 Research Link National University of Singapore Singapore 117604 Tel: 65-6872-7016, -7742/-7710 (lab) Fax: 65-6872-7016, -7007 (shared) http://www.tll.org.sg/itot.asp From Paul.Devlin from rhul.ac.uk Mon Jun 2 14:11:08 2008 From: Paul.Devlin from rhul.ac.uk (Devlin Paul) Date: Mon Jun 2 23:39:29 2008 Subject: [Arabidopsis] Plant Circadian Biology Postdoctoral Position Message-ID: Role of FHY3 and FAR1 in transcriptional regulation of circadian clock resetting by red light Ref KB/4985 Applications are invited for a Postdoctoral Research Assistant to work in the School of Biological Sciences, Royal Holloway University of London, under the supervision of Dr Paul Devlin. Salary for this post is ?25,136 per annum inclusive of London Allowance. The post is available after the 1st July 2008 and is for 36 months. The project involves investigation of the role of two transcription factors, FHY3 and FAR1, in light input to the plant circadian clock. This is a collaborative project with Dr Haiyang Wang, Boyce Thompson Institute for Plant Research, Ithaca, NY and will also involve spending three months at The Boyce Thompson Institute. For background information, see Allen et al., 2006, Plant Cell, 18, 2506-2516 and Lin et al., 2007, Science, 318, 1302-5. The investigation will involve a range of techniques including luciferase imaging; in vivo binding and activation assays; inducible expression analysis; mutant studies; promoter dissection; and protein interaction studies. Candidates will have a PhD in Plant Molecular Biology, or will have submitted a thesis prior to taking up the appointment. A background in plant molecular genetics and a strong interest in circadian biology are essential. Experience in any of the techniques involved would also be an advantage. Informal enquiries should be addressed to Dr Paul Devlin paul.devlin@rhul.ac.uk Further details and an application form are available at http://www.rhul.ac.uk/personnel/Ads/bio4985.html or from Personnel Department, Royal Holloway, University of London, Egham, Surrey TW20 0EX; fax: +44 (0)1784 274900 telephone: +44 (0)1784 414241 email: recruitment@rhul.ac.uk Please quote the reference number KB/4985 Closing date for receipt of applications is midday, 30th June 2008. Interviews are due to take place early July 2008 Dr Paul Devlin School of Biological Sciences Royal Holloway, University of London Egham Surrey TW20 0EX Tel: +44 (0)1784 414698 Fax: +44 (0)1784 414224 Email: paul.devlin@rhul.ac.uk From grelon from versailles.inra.fr Tue Jun 3 02:38:53 2008 From: grelon from versailles.inra.fr (Mathilde GRELON) Date: Tue Jun 3 11:15:21 2008 Subject: [Arabidopsis] post-doc position Message-ID: POST-DOCTORAL FELLOWSHIP A postdoctoral position in Molecular Biology and Genetics is available at INRA of Versailles. Post Doc Project: Meiotic recombination initiation mechanisms in Arabidopsis thaliana In budding yeast, meiotic recombination events are initiated by DNA double strand breaks (DSBs) catalysed by the Spo11 protein. In this species, DSB formation also requires nine other proteins whose molecular function is poorly understood. Nevertheless, recent studies tend to group them into subcomplexes, indicating that they can be intimately linked and can form a large recombination initiation complex in which DSBs are made. Many investigations have been carried out to identify in other organisms, homologs of these DSB forming proteins. Unfortunately, unlike Spo11, most of these are not conserved across kingdoms. Furthermore, even when DSB proteins are conserved, their role in meiotic DSB formation is missing. A large-scale forward genetic approach to isolate plant meiotic genes has revealed the existence of new meiotic functions that could be necessary for meiotic DSB formation in the model plant Arabidopsis thaliana. The selected candidate will be in charge of the cloning and the characterisation of several of these genes at the molecular, genetic and cytological levels. A large range of tools are already available in the laboratory (as chromosome structure or meiotic recombination progression markers), while others will be developed by the selected candidate in order to analyse the function of the isolated DSB forming proteins, as well as their in vitro or in vivo interactions. Candidate profile Applicants should have completed their PhD less than 5 years ago. A strong background in molecular biology, genetics or cytology will be appreciated. The position is available before the end of the year for a period of two years. If interested, please send an application outlining research experience and interests, curriculum vitae, and the names and addresses of two references to Dr. Mathilde Grelon (grelon@versailles.inra.fr), Institut Jean-Pierre Bourgin, INRA de Versailles, Station de G?n?tique et d'Am?lioration des Plantes, UR-254, Route de Saint-Cyr, F-78026 Versailles, France. Phone: (+33)(0)1.30.83.33.08. Fax:(+33)(0)1.30.83.33.19 Significant recent publications of the group - Mercier R., Grelon M. (2008) Meiosis in plants: ten years of gene discovery. Cytogenetics and Genome Research. Reviews in Plant Cytogenetics. 120(3-4):281-290. - de Muyt A., Vezon D., Gendrot G., Gallois J.-L., Stevens R., Grelon M. (2007) AtPRD1 is required for meiotic double strand break formation in Arabidopsis thaliana. EMBO J. 2007 Sep 19; 26(18):4126-37 - Chelysheva L., Gendrot G., Vezon D., Doutriaux M.-P., Mercier R., Grelon M. (2007) Zip4/Spo22 is required for class I CO formation but not for synapsis completion in Arabidopsis thaliana. PLoS Genet. 2007 May 25;3(5):e83. - Chelysheva L., Diallo S., Vezon D., Gendrot G., Vrielynck N., Belcram K., Rocques N., M?rquez-Lema A., Bhatt A. M., Horlow C., Mercier R., M?zard C, Grelon M. (2005) AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis. J. Cell Sci. Oct 15;118(Pt 20):4621-32 -Grelon M, Vezon D, Gendrot G, Pelletier G. (2001) AtSPO11-1 is neccessary for efficient meiotic recombination in plants. EMBO J. Feb 1;20(3):589-600. -- ******************************************************************* Dr. Mathilde Grelon Institut Jean-Pierre Bourgin INRA de Versailles Station de Genetique et d'Amelioration des Plantes UR-254 Route de Saint-Cyr F-78026 Versailles France Phone: (33).1.30.83.33.08 Fax: (33).1.30.83.33.19 ****************************************************************** From ZIMMERL from si.edu Tue Jun 3 09:07:39 2008 From: ZIMMERL from si.edu (Zimmer, Elizabeth (Liz)) Date: Tue Jun 3 11:15:27 2008 Subject: [Arabidopsis] technician position available Message-ID: Biological Science Technician SALARY RANGE: 39,330.00 - 62,546.00 USD per year OPEN PERIOD: Tuesday, May 27, 2008 to Tuesday, June 17, 2008 SERIES & GRADE: GS-0404-07/07 POSITION INFORMATION: Full Time Career/Career-Conditional PROMOTION POTENTIAL: 09 DUTY LOCATIONS: few vacancies - Suitland, MD WHO MAY BE CONSIDERED: Citizens or nationals of the United States. This position is also being announced under Agency Merit Promotion, Ann# 08A-RB-294018-MPA-NMNH. To apply, go to USAjobs.gov and search on "NMNH botany" For more information, contact Liz Zimmer at zimmerl@si.edu, Ken Wurdack at wurdackk@si.edu, Jun Wen at wenj@si.edu, Lee Weigt at weigtl@si.edu, Warren Wagner at wagnerw@si.edu, funkv@si.edu or kressj@si.edu . JOB SUMMARY: The Smithsonian Institution is a unique working and learning environment that offers you continuing awareness of the world's treasures in culture, art, and science. If your goals include challenging work, being a valued member of an organization, and participation in exciting opportunities of exploration, become a part of our staff. Enjoy family friendly benefits; a complimentary membership to the Resident Associate Program with discounts on thousands of educational and cultural programs offered annually; discounts at Smithsonian membership programs, shops, and restaurants; and much more! This position is located in National Museum of Natural History, Laboratories of Analytical Biology (LAB). The purpose of this position is to assist with the development of molecular genetic research and laboratory protocols. This position would be especially suitable for recent undergraduates or graduate students. KEY REQUIREMENTS: U.S. Citizenship or U.S. National status required. Post-appointment background investigation is required. You will be required to serve a one-year probationary period. This position has a career progression to GS-404-8/GS-404-9 MAJOR DUTIES: The duties of this position include, but are not limited to the following: - Conducts plant DNA isolations. - Performs polymerase chain reactions (PCR) in laboratory. - Analyzes PCR and DNA sequence products. - Completes AFLP, DNA fragment, microsatellite and primer work. - Conducts computer analysis of molecular data. - Performs initial estimation of genetic differentiation in species or populations. - Carries out molecular genetic laboratory experiments pertinent to various research projects of the laboratory and Department of Botany Scientists. - Assists in the training of students, interns and other laboratory staff. Elizabeth Anne Zimmer, Ph.D. Principal Investigator/Research Biologist Department of Botany Laboratories of Analytical Biology National Museum of Natural History, Smithsonian Institution Museum Support Center, MRC 534 4210 Silver Hill Rd. Suitland, MD 20746 Phone: 301-238-1118 FAX: 301-238-3059 Cell: 703-863-9806 email: zimmerl@si.edu URL: http://www.lab.si.edu/zimmer.html From esther.yakir from mail.huji.ac.il Tue Jun 3 11:04:01 2008 From: esther.yakir from mail.huji.ac.il (Esther Yakir) Date: Tue Jun 3 11:15:31 2008 Subject: [Arabidopsis] phospho-serine antibodies Message-ID: <43860b510806030904u4e6116e6u6ce8b0a5b0b240ce@mail.gmail.com> Hallo all Has anyone successfully used commercially available phospho-serine antibodies in westerns/immunoprecipitations of specific phosphorylated Arabidopsis proteins? Thanx Eti Yakir Dept Plant and Environmental Sciences, Hebrew University, Jerusalem, 91904 Israel From sean.cutler from ucr.edu Fri Jun 6 12:32:13 2008 From: sean.cutler from ucr.edu (Sean Cutler) Date: Fri Jun 6 14:38:06 2008 Subject: [Arabidopsis] Screening library for plant chemical genomics Message-ID: <4849749D.8080409@ucr.edu> Hello everyone- Over the past few years my lab has assembled a collection of ~3600 bioactive small molecules tailored for use in plant chemical genomics. We have shared this library with several labs to assist their screening initiatives and this email is to notify any interested users of its availability. More information about the library can be found at: http://cutlerlab.blogspot.com/2008/05/latca.html. Thanks- Sean Cutler -- Assistant Professor University of California, Riverside Department of Botany and Plant Sciences Center for Plant Cell Biology Department of Chemistry (CFM) 5451 Boyce Hall Riverside, CA 92521 (office) 951.827.6990 (lab) 951.827.6991 (fax) 951.827.4437 http://cutlerlab.blogspot.com/ From hudmatt from gmail.com Fri Jun 6 16:03:55 2008 From: hudmatt from gmail.com (Matt Hudson) Date: Fri Jun 6 16:23:53 2008 Subject: [Arabidopsis] Postdoctoral and graduate student positions in biofuel feedstock genomics Message-ID: <2ecd04bc0806061403p363b5cb8x278e1af5c0cbe94d@mail.gmail.com> A postdoctoral research position and graduate student assistantship are available immediately as part of the Feedstock Genomics program of the Energy Biosciences Institute at the University of Illinois, Urbana-Champaign. Both positions are to develop bioinformatics tools and to perform sequence assembly, analysis and visualization on resource-poor species utilizing next-generation sequencing techniques. The focus of the analysis will be on forthcoming sequences from Miscanthus, Sugar Cane and other Andropogoneae grass species with potential as lignocellulosic feedstocks. For the postdoctoral position, experience with plant genome sequence analysis is essential, and high throughput bioinformatics analysis skills are highly desirable. For the graduate assistantship a strong interest in computational biology and a background (MS preferred or BS) in either computer science or biology is necessary. The Andropogoneae Feedstock Genomics program is a collaboration between the laboratories of Steve Moose, Ray Ming and Matt Hudson at the University of Illinois, and Dan Rokhsar at the DoE Joint Genome Institute. The successful candidates for these positions will be located at the Urbana-Champaign branch of the Energy Biosciences Institute, which is funded by BP, plc. From sean.cutler from ucr.edu Tue Jun 10 17:20:34 2008 From: sean.cutler from ucr.edu (Sean Cutler) Date: Tue Jun 10 18:22:32 2008 Subject: [Arabidopsis] URL correction Message-ID: <484EFE32.4080801@ucr.edu> Hello everyone, There was a problem with the URL in the archived version of my prior post regarding LATCA. The correct url is: http://cutlerlab.blogspot.com/2008/05/latca.html Thanks- Sean Cutler -- Assistant Professor University of California, Riverside Department of Botany and Plant Sciences Center for Plant Cell Biology Department of Chemistry (CFM) 5451 Boyce Hall Riverside, CA 92521 (office) 951.827.6990 (lab) 951.827.6991 (fax) 951.827.4437 http://cutlerlab.blogspot.com/ From gzolla from yahoo.com Wed Jun 11 06:30:51 2008 From: gzolla from yahoo.com (Gaston Zolla) Date: Thu Jun 12 11:22:12 2008 Subject: [Arabidopsis] Brassinosteroids signal Message-ID: <313699.71644.qm@web31706.mail.mud.yahoo.com> Hi Does anybody know of an inhibitor for Brassinosteroid signal? thank in advance Gaston From wyatts from ohio.edu Wed Jun 11 14:20:15 2008 From: wyatts from ohio.edu (Sarah Wyatt) Date: Thu Jun 12 11:22:31 2008 Subject: [Arabidopsis] Graduate position available Message-ID: <4850256F.5000607@ohio.edu> /Plant growth and development/: Research and teaching assistantships are still available. The major goal of the research is to use molecular genetics, physiology and bioinformatics to identify/characterize the molecular components that link gravity stimulus perception and the differential growth response. The successful candidate must be accepted to either the interdisciplinary Molecular and Cell Biology Graduate Program or the Environmental and Plant Biology Graduate Program at Ohio University. For more information contact Dr. Sarah Wyatt, Environmental and Plant Biology, Ohio University, Athens, OH (wyatts@ohio.edu ), http://oak.cats.ohiou.edu/wyatts/wylab.html . -- ______________________________________________________________________ Sarah E. Wyatt, PhD Associate Professor Enivornmental and Plant Biology 315 Porter Hall 22 Richland Ave. Ohio University Athens, OH 45701 (740) 593-1133 Phone (740) 593-1130 FAX From anirbanb from ncbs.res.in Fri Jun 13 04:50:16 2008 From: anirbanb from ncbs.res.in (Anirban Baral) Date: Fri Jun 13 18:44:34 2008 Subject: [Arabidopsis] sterilization of arabidopsis seeds Message-ID: <52775.192.168.1.1.1213350616.squirrel@mail.ncbs.res.in> Dear Arabineters, can you suggest me a conveient way to surface sterilize Arabidopsis seeds,this is meant to grow calli out of seedlings. Please mail me to anirbanb@ncbs.res.in Thanks in advance -- Anirban Baral JUNIOR SCHOLAR NATIONAL CENTRE FOR BIOLOGICAL SCIENCES TATA INSTITUTE OF FUNDAMENTAL RESEARCH UAS-GKVK CAMPUS,BELLARY ROAD BANGALORE-560065,INDIA From bsterlinger from gmail.com Fri Jun 13 09:18:00 2008 From: bsterlinger from gmail.com (bsterlinger@gmail.com) Date: Fri Jun 13 18:45:12 2008 Subject: [Arabidopsis] How to Take Closeup Pics on Arabidopsis Flowers Message-ID: <1ef42a7e-9a5f-4bb0-be79-e94ca2d7e6c3@25g2000hsx.googlegroups.com> Hi everybody: I am trying to use a digital camera (Olympus Camedia C-5050 ZOOM) to take a closeup pictures of Arabidopsis flowers and siliques. The camera can take pictures at as close as 3 cm to an object. But when I take a closeup photo and blow it up in Word or PowerPoint, it loses some resolution. I want to take a picture and use it for publication using a digital camera. Please help and thank you for your time. Cheers, Bruce From carl.pike from fandm.edu Fri Jun 13 07:12:06 2008 From: carl.pike from fandm.edu (Carl Pike) Date: Fri Jun 13 18:45:45 2008 Subject: [Arabidopsis] Gateway-compatible Vector Message-ID: <42242AD4-A5B0-4002-B6B6-93DC290DB20D@fandm.edu> I have a gene which is in the Gateway entry vector pENTR/SD-DTOPO. My understanding (as a newcomer to Gateway technology) is that this entry vector can be used to produce C-terminal fusions, but not for N- terminal fusions. I would like to produce a his-tagged protein, but a destination vector is only available commercially for the N- terminal his-tagged fusion. Does anyone know of a destination vector available for producing the C-terminal his tagged recombinant using my entry vector and the commercially available recombinase? Thank you. Carl Pike Franklin and Marshall College From aboisson from biomail.ucsd.edu Fri Jun 13 21:58:19 2008 From: aboisson from biomail.ucsd.edu (Aurelien BOISSON) Date: Sat Jun 14 00:07:18 2008 Subject: [Arabidopsis] Roomate for the XX International Congress on Sexual Plant Reproduction? Message-ID: Hi everybody, is there anyone who would be interested in sharing a hotel room with me at the XX International Congress on Sexual Plant Reproduction to be held in Bras?lia August 4-8, 2008 ? Please contact me by email at aboisson@ucsd.edu, Best regards, Aurelien Aurelien BOISSON, Ph.D. Julian Schroeder's Lab, Division of Biological Sciences, University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0116, USA Tel: (858) 534 7432 Fax: (858) 534 7108 Email: aboisson@biomail.ucsd.edu http://www-biology.ucsd.edu/labs/schroeder/index.html From Christian.Hardtke from unil.ch Sun Jun 15 06:16:59 2008 From: Christian.Hardtke from unil.ch (Christian Hardtke) Date: Sun Jun 15 14:51:54 2008 Subject: [Arabidopsis] Post-doc in Plant Growth & Development, Lausanne, Switzerland Message-ID: <0768260C-C788-4B29-9F2C-7CB559B7580B@unil.ch> A postdoctoral position is available starting in October 2008 or later in the lab of Christian Hardtke at the Department of Plant Molecular Biology, University of Lausanne, Switzerland. Our department offers excellent research infrastructure combined with a lively, interactive and international scientific community, located in the beautiful Lake Geneva region. This position is funded by the University of Lausanne and includes a light teaching load (supervision and partial preparation of a practical, B.Sc. level lab course once per year). The candidate is expected to build on recent progress in our lab along the lines of one of our main projects: 1. Biochemical and cell biological analysis of transcriptional and post-translational hormonal control of the BRX family and interacting proteins in Arabidopsis development (building on Mouchel et al, Nature, 2006; & recent progress). 2. Molecular genetic analysis of natural variation in secondary growth of the Arabidopsis vasculature (building on Sibout et al., Current Biology, 2008; & recent progress). 3. Investigation of the impact of natural structural genome variation on plant performance (building on Shindo et al., New Phytologist, in press; & recent progress). This project requires a pronounced interest and ideally demonstrated expertise in bioinformatics approaches. More details about the individual projects are available upon request. Strongly motivated candidates with demonstrated expertise in molecular techniques are encouraged to apply. Please forward applications including a curriculum vitae and contact information for two senior scientist referees by e-mail to christian.hardtke@unil.ch. lab web page: www.unil.ch/dbmv/page21142_en.html From Christian.Hardtke from unil.ch Sun Jun 15 06:17:31 2008 From: Christian.Hardtke from unil.ch (Christian Hardtke) Date: Sun Jun 15 14:52:02 2008 Subject: [Arabidopsis] Post-doc in Plant Cell Biology, Lausanne, Switzerland Message-ID: <0B2AF1DF-0E0E-47FE-AA66-6A1E1ABC7A8D@unil.ch> A postdoctoral position is available starting immediately in the lab of Christian Hardtke at the Department of Plant Molecular Biology, University of Lausanne, Switzerland. Our department offers excellent research infrastructure combined with a lively, interactive and international scientific community, located in the beautiful Lake Geneva region. The candidate for this SNF-funded position is expected to build on recent progress in our lab to investigate the role of BRX family proteins in various aspects of growth and development in Arabidopsis and other species, with an emphasis on the transcriptional and post- translational hormonal control of BRX and its interaction partners (building on Mouchel et al, Genes & Development, 2004; Mouchel et al., Nature, 2006; & recent progress). More details about the project are available upon request. Strongly motivated candidates with demonstrated expertise in molecular techniques are encouraged to apply. Please forward applications including a curriculum vitae and contact information for two senior scientist referees by e-mail to christian.hardtke@unil.ch. lab web page: www.unil.ch/dbmv/page21142_en.html From Christian.Hardtke from unil.ch Sun Jun 15 06:18:33 2008 From: Christian.Hardtke from unil.ch (Christian Hardtke) Date: Sun Jun 15 14:52:07 2008 Subject: [Arabidopsis] Ph.D. position in Plant Systems Biology, Lausanne, Switzerland Message-ID: <02CB68C0-0C05-4DB2-8D28-329387A6C4F0@unil.ch> A Ph.D. student position is available starting immediately in the lab =20= of Christian Hardtke at the Department of Plant Molecular Biology, =20 University of Lausanne, Switzerland. Our department offers excellent =20 research infrastructure combined with a lively, interactive and =20 international scientific community, located in the beautiful Lake =20 Geneva region. This position is part of the larger =93Plant Growth in a Changing =20 Environment=94 network of SystemsX, the Swiss Systems Biology = initiative. The candidate will work on two inter-connected sub-projects: 1. Comprehensive investigation of the genetic and environmental =20 parameters that determine later stage secondary growth vigor of the =20 Arabidopsis vasculature (building on Sibout et al., Current Biology, =20 2008; & recent progress). 2. Quantitative modeling of a hormone-controlled, multi-level =20 feedback loop that determines the homeostasis of the activity of a =20 pivotal regulator of vascular growth (building on recent progress). More details about the project are available upon request. Strongly motivated candidates with a solid background knowledge in =20 molecular techniques and a strong interest in bioinformatics and =20 modeling approaches are encouraged to apply. Please forward applications including a curriculum vitae and contact =20 information for two senior scientist referees by e-mail to =20 christian.hardtke@unil.ch. lab web page: www.unil.ch/dbmv/page21142_en.html From Jose.Gonzalez from sdstate.edu Mon Jun 16 09:30:40 2008 From: Jose.Gonzalez from sdstate.edu (Gonzalez, Jose) Date: Mon Jun 16 15:59:40 2008 Subject: [Arabidopsis] Research Associate I position available. Message-ID: <5323AEB9BA07874090D896D29441D6C601C92DD5@ATLANTIS-EVS.jacks.local> Please read the ad carefully. RESEARCH ASSOCIATE I Plant Science Department South Dakota State University A full-time 12-month Research Associate I position within the Plant Science Department of South Dakota State University. This is a grant funded position, annual reappointment contingent upon availability of funds and satisfactory performance. DUTIES AND RESPONSIBILITIES: The incumbent will primarily be responsible for the autonomous coordination, conduction, and reporting of the molecular biology component within the Seed Molecular Biology group. This includes the oversight of duties carried out by hourly and/or temporary employees as well as graduate students and other program contributors. Specific duties associated with this component will entail, though not be limited to, design and conduct experiments to meet research goals and to prepare grant proposals and progress reports. REQUIRED QUALIFICATIONS: An MS degree in Plant Science, Plant Pathology, Plant Molecular Biology or related field. Professional experience in molecular biology after the MS degree is required. Additional requirements include effective interpersonal, oral and written communication skills; and willingness to work flexible hours when necessary. PREFERRED QUALIFICATIONS: (1) diverse PCR protocols, (2) DNA, RNA and protein extraction, electrophoresis and hybridization, (3) working in sterile conditions with bacterial cultures or plant tissue culture, (4) growing plants in greenhouse and field, (5) writing grants and /or scientific publications, (6) experimental design and data analysis, and (7) laboratory management, including personnel supervision. Familiarity with the molecular biology of two or more biological systems in addition to protocol development and troubleshooting are also highly desirable qualities. SALARY: Salary will be commensurate with qualifications. UNIVERSITY/COMMUNITY: SDSU is located in Brookings, South Dakota, a community of approximately 18,800 near the east central border of South Dakota on Interstate 29. The city has an excellent K-12 education system, is accessible to major medical facilities, has an active cultural and social environment, and has numerous lakes and parks within driving distance. It is 50 miles north of Sioux Falls, a city of close to 150,000. APPLICATION DEADLINE: July 15, 2008 APPLICATION PROCESS: To apply, visit the on-line employment site at: https://yourfuture.sdbor.edu, search by the position title, view the job announcement, and click on "apply for this posting." The system will guide you through the electronic application form. The employment site will also require the attachment of a cover letter (a statement explaining how you meet required and preferred qualification is required), resume, and a list of professional references. Applications should present an accurate description of abilities, knowledge, skills, and interests relevant to the qualifications. For questions on the position, contact Dr. Jose L. Gonzalez ( jose.gonzalez@sdstate.edu) (605-688-6907). For assistance with the on-line employment process, contact SDSU Human Resources at (605) 688-4128. South Dakota State University is committed to affirmative action, equal opportunity and the diversity of its faculty, staff and students. Women and minorities are encouraged to apply. Arrangements for accommodations required by disabilities can be made at TTY (605) 688-4394. SDSU prohibits discrimination on the basis of race, color, creed, religion, national origin, ancestry, citizenship, gender, marital status, pregnancy, sexual orientation, age, disability, veteran's status or any other protected class in the offering of all educational programs and employment opportunities. Individuals with concerns regarding discrimination should contact: Equal Opportunity Officer/Title IX Coordinator, Human Resources, Administration 324, SDSU, Brookings, SD 57007. Jos? L. Gonz?lez Jose L. Gonzalez Assistant Professor. Dept. of Plant Sciences South Dakota State University Brookings, SD 57007 USA Phone (office):605-688-6907 Fax: 605-688-4452 http://plantsci.sdstate.edu/gonzalez/gonzalez lab.htm From kbrowning from mail.utexas.edu Tue Jun 17 11:33:11 2008 From: kbrowning from mail.utexas.edu (Karen Browning) Date: Tue Jun 17 12:17:13 2008 Subject: [Arabidopsis] antibodies to modifications--any work in Arabidopsis? Message-ID: <4857E747.4060303@mail.utexas.edu> Does anyone have experience using commercial antibodies to protein modifications (acetylation, methylation, glycosylation, sumoylation etc) with Arabidopsis or other plant protein targets? If so, please send the names of the company and specific antibody used. Thank you. -- *********************************************************** Dr. Karen S. Browning Associate Professor Department of Chemistry and Biochemistry The University of Texas at Austin Welch Hall 5.248, 24th and Speedway (Overnight Deliveries) 1 University Station A5300 (Regular Mail) Austin TX 78712-0165 kbrowning@mail.utexas.edu http://research.cm.utexas.edu/kbrowning/ 512-471-4562 512-471-8696 FAX From Nemo.Peeters from toulouse.inra.fr Wed Jun 18 05:41:15 2008 From: Nemo.Peeters from toulouse.inra.fr (Nemo Peeters) Date: Wed Jun 18 11:33:37 2008 Subject: [Arabidopsis] Postdoctoral position in plant-microbe interaction, at LIPM, Toulouse, France Message-ID: <4858E64B.2060405@toulouse.inra.fr> 19-months postdoc position, at LIPM, Toulouse, France To study the mode of action of the F-box "GALA" type III effector proteins of Ralstonia solanacearum on Arabidopsis. Available from 1st September 2008 (starting no later than 1st January 2009) Location: The LIPM (www.toulouse.inra.fr/centre/lipm), in Toulouse, France, is a dynamic and international group of researchers studying plant-microbe interactions. It provides an excellent scientific environment and facilities to carry out this project. The Position: Together with the project leader, a PhD student and technical assistance, the candidate will carry out Arabidopsis molecular genetics, bacterial infection studies, protein biochemistry in order to identify and characterize the targets of the GALA proteins injected by R. solanacearum in plant cells. Annual Gross salary of 25 611 EUR (expected Net salary around 1800 EUR/month including health and unemployment benefits). Starting the 1^st of February 2008 or soon after. The candidate: Should have experience in plant molecular genetics and preferably also protein biochemistry The candidate is expected to have a strong enthusiasm (not necessary previous experience) for plant-pathogenic bacteria interactions. Good English scientific communication skills are essential. Application: Please send email inquiry and application, with subject header "postdoc GALA", to Nemo Peeters (peeters@toulouse.inra.fr, tel:+33561285592). Application should contain a cover letter outlining previous experience, a curriculum vitae and up to three reference contact details. Living in Toulouse: Fourth largest city in France, Toulouse is attractive in terms of cultural and sport activities. The South of France is renowned for its quality of life. References: *Kajava, AV., Anisimova, M. and Peeters, N. (2008). Origin and evolution of GALA-LRR, a new member of the CC-LRR subfamily: from plants to bacteria? PLoS ONE 3(2): e1694. *Angot, A., Vergunst, A., Genin, S., and Peeters, N. (2007). Exploitation of eukaryotic ubiquitin signaling pathways by effectors translocated by bacterial type III and type IV secretion systems. PLoS Pathog /3/, e3. *Angot, A., Peeters, N., Lechner, E., Vailleau, F., Baud, C., Gentzbittel, L., Sartorel, E., Genschik, P., Boucher, C., and Genin, S. (2006). /Ralstonia solanacearum /requires F-box-like domain-containing type III effectors to promote disease on several host plants. Proc Natl Acad Sci U S A /103/, 14621-14625. From qin.zeng from monsanto.com Wed Jun 18 13:16:36 2008 From: qin.zeng from monsanto.com (ZENG, QIN [AG/1005]) Date: Wed Jun 18 15:52:15 2008 Subject: [Arabidopsis] alternatives to chloral hydrate Message-ID: <37AF799DE152DB48B1DBD42B6311190504FBA523@NA1000EXM04.na.ds.monsanto.com> If you have answer for the following questions, I would really appreciate it. What is the history of chloral hydrate being used as the tissue clearing agent? What are the tissue clearing mechanisms for chloral hydrate? Are there any other chemicals that have equivalent functions? Thanks, Qin ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Qin Zeng, Ph.D 700 Chesterfield Village Parkway, GG5181-D Chesterfield, MO 63017 (636)-737-6970 --------------------------------------------------------------------------------------------------------- This e-mail message may contain privileged and/or confidential information, and is intended to be received only by persons entitled to receive such information. If you have received this e-mail in error, please notify the sender immediately. Please delete it and all attachments from any servers, hard drives or any other media. Other use of this e-mail by you is strictly prohibited. All e-mails and attachments sent and received are subject to monitoring, reading and archival by Monsanto, including its subsidiaries. The recipient of this e-mail is solely responsible for checking for the presence of "Viruses" or other "Malware". Monsanto, along with its subsidiaries, accepts no liability for any damage caused by any such code transmitted by or accompanying this e-mail or any attachment. --------------------------------------------------------------------------------------------------------- From chinnatal from gmail.com Thu Jun 19 02:58:26 2008 From: chinnatal from gmail.com (channa keshava) Date: Thu Jun 19 13:19:18 2008 Subject: [Arabidopsis] Re: Arab-gen Digest, Vol 38, Issue 9 In-Reply-To: <200806181703.m5IH3fO15865@net.bio.net> References: <200806181703.m5IH3fO15865@net.bio.net> Message-ID: <1f32ce730806190058p13ad4b37g6ce825331c8bd10c@mail.gmail.com> I am a PhD student in Prof. Huelskamp lab, Cologne, Germany. I am in need of Arabidopsis wildtype seeds of Nossen (No-0) ecotype. If anybody has please send it to me soon. Channa Keshavaiah Botanical Institute-III Gyrhofstrasse-15, 50931 University of Cologne, Cologne Germany On 6/18/08, arab-gen-request@oat.bio.indiana.edu < arab-gen-request@oat.bio.indiana.edu> wrote: > > Send Arab-gen mailing list submissions to > arab-gen@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/arab-gen > or, via email, send a message with subject or body 'help' to > arab-gen-request@net.bio.net > > You can reach the person managing the list at > arab-gen-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Arab-gen digest..." > > > Today's Topics: > > 1. antibodies to modifications--any work in Arabidopsis? > (Karen Browning) > 2. Postdoctoral position in plant-microbe interaction, at LIPM, > Toulouse, France (Nemo Peeters) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 17 Jun 2008 11:33:11 -0500 > From: Karen Browning > Subject: [Arabidopsis] antibodies to modifications--any work in > Arabidopsis? > To: arab-gen@magpie.bio.indiana.edu > Message-ID: <4857E747.4060303@mail.utexas.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Does anyone have experience using commercial antibodies to protein > modifications (acetylation, methylation, glycosylation, sumoylation etc) > with Arabidopsis or other plant protein targets? > > If so, please send the names of the company and specific antibody used. > Thank you. > -- > *********************************************************** > Dr. Karen S. Browning > Associate Professor > Department of Chemistry and Biochemistry > The University of Texas at Austin > Welch Hall 5.248, 24th and Speedway (Overnight Deliveries) > 1 University Station A5300 (Regular Mail) > Austin TX 78712-0165 > > kbrowning@mail.utexas.edu > http://research.cm.utexas.edu/kbrowning/ > > 512-471-4562 > 512-471-8696 FAX > > > > ------------------------------ > > Message: 2 > Date: Wed, 18 Jun 2008 12:41:15 +0200 > From: Nemo Peeters > Subject: [Arabidopsis] Postdoctoral position in plant-microbe > interaction, at LIPM, Toulouse, France > To: Arab-gen@magpie.bio.indiana.edu > Message-ID: <4858E64B.2060405@toulouse.inra.fr> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > 19-months postdoc position, at LIPM, Toulouse, France > To study the mode of action of the F-box "GALA" type III effector > proteins of Ralstonia solanacearum on Arabidopsis. > > Available from 1st September 2008 (starting no later than 1st January 2009) > > Location: > The LIPM (www.toulouse.inra.fr/centre/lipm), in Toulouse, France, is a > dynamic and international group of researchers studying plant-microbe > interactions. It provides an excellent scientific environment and > facilities to carry out this project. > > The Position: > Together with the project leader, a PhD student and technical > assistance, the candidate will carry out Arabidopsis molecular genetics, > bacterial infection studies, protein biochemistry in order to identify > and characterize the targets of the GALA proteins injected by > R. solanacearum in plant cells. > > Annual Gross salary of 25 611 EUR (expected Net salary around 1800 > EUR/month including health and unemployment benefits). > Starting the 1^st of February 2008 or soon after. > > The candidate: > Should have experience in plant molecular genetics and preferably also > protein biochemistry The candidate is expected to have a strong > enthusiasm (not necessary previous experience) for plant-pathogenic > bacteria interactions. Good English scientific communication skills are > essential. > > Application: > Please send email inquiry and application, with subject header "postdoc > GALA", to Nemo Peeters (peeters@toulouse.inra.fr, tel:+33561285592). > Application should contain a cover letter outlining previous experience, > a curriculum vitae and up to three reference contact details. > > Living in Toulouse: > Fourth largest city in France, Toulouse is attractive in terms of > cultural and sport activities. The South of France is renowned for its > quality of life. > > > References: > *Kajava, AV., Anisimova, M. and Peeters, N. (2008). Origin and evolution > of GALA-LRR, a new member of the CC-LRR subfamily: from plants to > bacteria? PLoS ONE 3(2): e1694. > > *Angot, A., Vergunst, A., Genin, S., and Peeters, N. (2007). > Exploitation of eukaryotic ubiquitin signaling pathways by effectors > translocated by bacterial type III and type IV secretion systems. PLoS > Pathog /3/, e3. > > *Angot, A., Peeters, N., Lechner, E., Vailleau, F., Baud, C., > Gentzbittel, L., Sartorel, E., Genschik, P., Boucher, C., and Genin, S. > (2006). /Ralstonia solanacearum /requires F-box-like domain-containing > type III effectors to promote disease on several host plants. Proc Natl > Acad Sci U S A /103/, 14621-14625. > > > > > > > > ------------------------------ > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > End of Arab-gen Digest, Vol 38, Issue 9 > *************************************** > From baskin from bio.umass.edu Thu Jun 19 14:31:40 2008 From: baskin from bio.umass.edu (Tobias Baskin) Date: Thu Jun 19 18:55:03 2008 Subject: [Arabidopsis] postdoc opening Message-ID: Postdoctoral Position Auxin transport in arabidopsis roots University of Massachusetts, Amherst, MA Bard College at Simon's Rock, Great Barrington, MA The labs of Eric Kramer (Bard College at Simon's Rock) and Tobias Baskin (University of Massachusetts, Amherst) have a position for a postdoctoral fellow. Kramer and Baskin have a joint, NSF-sponsored grant to study transport in the arabidopsis root, with a focus on auxin. Highest priority will be given to candidates with demonstrated skill in plant cell biology and microscopy. Experience with arabidopsis is NOT required (for all you maize folks). Experience with or willingness to learn mathematical/computer simulation techniques is preferred, but not essential. The postdoc will live near Amherst, MA, with occasional trips to Simon's Rock. The position starts Sept 1, 2008, and screening applicants will begin immediately. Initial appointment is for one year, with the possibility of renewal for up to three more years. Persons from groups under-represented in the sciences are strongly encouraged to apply. Contact: Send a single PDF file containing a cover letter, CV, publication list, and the names/emails of three references to Dr. Eric Kramer, ekramer@simons-rock.edu. -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 From jglazebr from umn.edu Thu Jun 19 18:28:14 2008 From: jglazebr from umn.edu (Jane Glazebrook) Date: Thu Jun 19 18:55:35 2008 Subject: [Arabidopsis] Post-doctoral position available Message-ID: <006c01c8d264$25be9a30$713bce90$@edu> POST-DOCTORAL POSITION IN ARABIDOPSIS DISEASE RESISTANCE Jane Glazebrook Laboratory Department of Plant Biology University of Minnesota St. Paul, Minnesota, USA 55108 A post-doctoral scientist is needed to study genetics of disease resistance in Arabidopsis thaliana. Part of the project involves study of genes required for resistance to the fungal necrotroph Alternaria brassicicola. Another part of the project involves investigation of the effects of cell wall alterations on resistance to various pathogens. The work provides opportunities to develop and enhance skills in genetics, molecular biology, and computational biology. Interested scientists may also participate in programs to develop teaching skills. The position is available beginning September 1, 2008, and is funded for three years. The University of Minnesota provides excellent research and educational opportunities. The successful applicant will be based primarily in the Center for Microbial and Plant Genomics on the St. Paul campus and will have convenient access to a diverse array of modern facilities. The Twin Cities area is often cited as providing one of the highest qualities of life among major US cities. Diverse cultural and recreational activities are readily available. Applicants should have a PhD and strong molecular biology and quantitative skills. To apply for this position, submit a curriculum vita (resum?), a letter describing your research interests and experience and the names and addresses of three references. The University of Minnesota is committed to the policy that all persons shall have equal access to its programs, facilities, and employment. Send application to: Jane Glazebrook (jglazebr@umn.edu) Jane Glazebrook, Ph.D. Associate Professor Department of Plant Biology University of Minnesota Rm 250 BioScience Center 1445 Gortner Ave. St. Paul, MN 55108 U.S.A. Phone: (612) 624-5194 Fax: (612) 624-6264 e-mail: jglazebr@umn.edu From peifenz from acoma.stanford.edu Thu Jun 19 17:10:04 2008 From: peifenz from acoma.stanford.edu (Peifen Zhang) Date: Thu Jun 19 18:56:07 2008 Subject: [Arabidopsis] Plant Metabolic Network News Announcement Message-ID: <485AD93C.6040608@acoma.stanford.edu> Dear Plant Researchers, The Plant Metabolic Network (PMN) is pleased to announce the initial launch of its official web site (http://plantcyc.org) and the first release of PlantCyc, a comprehensive biochemical pathway reference database dedicated to the plant kingdom. The PMN is an NSF-funded collaborative project among databases and biochemists with a common goal to build a broad network of plant metabolic pathway databases. The central database of PMN, PlantCyc (version 1.0), has over 500 pathways and about 3000 enzymes involved in primary and secondary metabolism, consolidated from over 290 plant species. The majority of these pathways come from AraCyc (version 4.5, http://www.arabidopsis.org/biocyc/index.jsp) and MetaCyc (version 12.0, http://metacyc.org). Many pathway diagrams were manually extracted from the plant literature. They are either supported by experimental evidence or are based on expert hypotheses. A small portion of the pathways were computationally predicted but validated by curators with supporting evidence in the literature. Evidence codes are attached to pathways and to enzymes to indicate data quality. In addition to PlantCyc, the PMN will develop and host a collection of single-species databases like that of AraCyc (Arabidopsis). We are currently developing databases for several agronomically important plants including poplar, soybean, wheat and maize. PMN will also host databases developed elsewhere such as RiceCyc (rice), MedicCyc (medicago) and LycoCyc (tomato). These single-species pathway databases place the sequenced and annotated genomes or ESTs in a biochemical context to facilitate the discovery of enzymes and the engineering of metabolic pathways. They also provide a platform for users to display and analyze functional genomics data, such as those generated from microarray and metabolomic experiments. Nearly 30 plant biochemists serve on the PMN editorial board to provide overall vision and guidance to the PMN project and they play an active role in ensuring the high quality of the information present in the PMN. The enhancement of the PMN databases is an ongoing effort. All users are encouraged to send corrections, feedback, and new data. Please contact us at curator@plantcyc.org. The PMN Team From kulbir18 from gmail.com Fri Jun 20 14:06:27 2008 From: kulbir18 from gmail.com (Kulbir Singh) Date: Fri Jun 20 17:16:34 2008 Subject: [Arabidopsis] Gus staining Message-ID: <34b347610806201206q74e73cb3t6b248221f10fc3a1@mail.gmail.com> Hello everybody, I have a problem in Gus staining. The problem is that the gene i am interested in, is expressed at low levels. So i dont see gus staining in the tissue where i expect to see it until i have a line with multiple copies of *Gene promoter:Gene:GUS* fusion construct. I have screened around 110 T2 lines to find around 7-8 homozygous lines in T3 generation with single locus insertion. But none of them expresses gene at a level to see the Gus staining in tissue i expect. Can anyone who had this problem suggest me what i can do in my situation. I am trying to screen more T2 lines by tranforming some plants again. But this will take few months to reach the T3 generation. Thanks, -- Kulbir Singh From eurus from psb.ugent.be Mon Jun 23 01:22:36 2008 From: eurus from psb.ugent.be (Eugenia Russinova) Date: Mon Jun 23 16:43:29 2008 Subject: [Arabidopsis] Post Doctoral Position Message-ID: <485F412C.9070803@psb.ugent.be> Dear colleagues, Could you please post our ad on your mailing list (see the text below)? With kind regards, Jenny Russinova CropDesign NV, located in Gent, Belgium is one of the worldwide leading , inoovative biotech companies, focusing on the improvement of crops. CropDesign has developed the high throughput phenotyping platform called TraitMill TM. This TraitMill platform is used to unravel the function of thousands of genes involved in yield and yield stability signalling pathways. CropDesign is a subsidiary of BASF Plant Science. Together with 6 other competitive research centres across the world, we form the global research platform of BASF Plant Science with the common goal to develop new improved products. Within the framework of the FP7 BRAVISSIMO project, coordinated by the Flemish Intstitute of Biotechnology (VIB, Gent, Belgium), CropDesign is looking for a young not graduated PhD student with a european non-belgian citizenship and with less that 4 years experience in Biology research. This BRAVISSIMO project is to discover new pathways involved in brassinosteroids in plants. The work to be done is directed towards crop improvement, mostly rice but with applications in other cereals and other crops such as corn and soybean. The student we are looking for should be very motivated, able to work in a group and in a company environment. Ideally the candidate would defend his/her PhD around December 2008. Please send curiculum vitae and letter of interest to Anja Beeckmans, anja.beeckmans@basf.com -- ================================================================== Jenny Russinova, PhD Tel:+32 (0)9 331 39 31 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM eurus@psb.ugent.be http://www.psb.ugent.be ================================================================== From nate.waldron from ocrp.org Wed Jun 25 10:18:29 2008 From: nate.waldron from ocrp.org (Nate Waldron) Date: Tue Jul 1 13:01:26 2008 Subject: [Arabidopsis] Arabidopsis Follow up: Rubisco Message-ID: <146953a30806250818g64263fe0wfb84278040616b58@mail.gmail.com> Hello, I found your posting about removing rubisco from plant extracts using PEI and I was wondering if it would be possible to get the protocols you used to develop the stock solution of PEI and the actual extraction protocol. I have been having a very similar problem with a protein I've been trying to extract/purify. My protein will only come out in alkaline pH's with fairly high salt concentrations, which, as you know, also extracts a whole lot of RubisCo. My protein is also very similar in size to Rubisco as well which has made it harder to purify. Anyway, if you wouldn't mind emailing a copy of the protocols I would really appreciate it. Thanks much. -Nate Waldron From vkalavacharla from desu.edu Wed Jun 25 07:45:01 2008 From: vkalavacharla from desu.edu (Venugopal Kalavacharla) Date: Tue Jul 1 13:01:44 2008 Subject: [Arabidopsis] Postdoctoral position-Plant Molecular Genetics, Dover, DE Message-ID: <005f01c8d6c1$4798af40$0c21a8c0@YOUR75A2502B97> Post Doctoral Research Associate-Plant Molecular Genetics, Delaware State University, Dover, DE The College of Agriculture and Related Sciences at Delaware State University is seeking qualified applicants for the position of Post Doctoral Research Associate, Plant Molecular Genetics. Applicants must have experience working with the genetics of plant host-pathogen interactions, construction of genomic and cDNA libraries; RNA interference is an added plus. Essential functions of the job include: 1. Conduct plant molecular genetics and genomics research and manage laboratory, greenhouse and growth room experiments. 2. Communicate effectively with faculty, students, University administrators and professional groups. 3. Provide leadership by encouraging excellence in research, education and outreach activities that are undertaken by the Plant Molecular Genetics and Genomics Group. 4. Mentor undergraduate and graduate students, technicians, and other laboratory staff in research programs. 5. Serve as a representative for activities of the Plant Molecular Genetics & Genomics Group and promote its best interests. Other functions: 1. Perform high school and community outreach. 2. Perform research in area(s) of this expertise. REQUIRED, KNOWLEDGE, SKILLS, and ABILITIES: 1. Research experience in molecular genetics and genomics. 2. Ability to work successfully with diverse students and faculty. 3. Effective written and verbal communication skills. QUALIFICATION STANDARDS: 1. Earned Ph.D. level degree and work experience in Plant Molecular Biology, Plant Molecular Genetics, or Genomics/Plant Pathology/Plant Biology. 2. Experience in advanced molecular genetic, genomic and proteomic techniques. 3. Added Plus: Prior experience in plant tissue culture, host-pathogen interactions and/or working with model or non-model plant species, management of laboratory, greenhouse, and growth room experiments and mentoring students. Letter of interest, resume and letters of recommendation can be submitted to: Dr. Venu Kalavacharla, Department of Agriculture and Related Sciences, Delaware State University, 1200 N. DuPont Highway, Dover, DE 19901. Phone: (302) 857-6492 or vkalavacharla@desu.edu. Delaware State University is an equal opportunity employer and does not discriminate because of race, creed, national or ethnic origin, sex or disability. Venu (Kal) Kalavacharla Ph.D. Plant Molecular Genetics & Genomics Department of Agriculture & Natural Resources Delaware State University Dover, DE 19901 Ph:(302) 857-6492 Fax:(302) 857-6402 Email: vkalavacharla@desu.edu