From owner-contents@net.bio.net Wed Feb 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Blood Cells, Molecules, & Diseases, vol. 24, no. 02, 31 January 1998 Date: 5 Feb 1998 10:32:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 108 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6bd0km$i39@net.bio.net> NNTP-Posting-Host: net.bio.net AU Trapp-OM. Beykirch-MK. Petrides-PE. TI Treatment of Patients with Chronic Myelogenous Leukemia and a High Platelet Count SO Blood-Cells-Mol-Dis. 1998 Jan 31. 24(2). P. 9-13. DE chronic myelogenous leukemia DE thrombocytosis DE anagrelide DE hydroxyurea AB Chronic myelogenous leukemia (CML) is usually treated with hydroxyurea or interferon-alpha. In some patients high platelet counts develop although leukocyte counts are well controlled with these drugs. If in such a situation cytoreductive therapy has to be intensified by a increase of the dosage, anemia and leukocytopenia as well as adverse effects of the drugs are likely to occur. In twelve CML patients we have therefore combined the basic CML treatment with anagrelide. This drug which selectively reduces platelet counts has been shown to be efficacious in the control of thrombocytosis in essential thrombocythemia. The diagnosis had been confirmed in all CML patients by cytogenetic and/or molecular biological analysis. The median age of our group was 58 years. Five were women and seven men. All patients were on treatment with hydroxyurea, some of them had previously received treatment with interferon-alpha (alone or in combination with hydroxyurea), busulfan or melphalan. Prior to the initiation of anagrelide treatment the platelet count was between 970,000 and 3,600,000/microl (median about 2,000,000/microl). Seven patients had thrombohemorrhagic complications. All twelve patients, experienced hematologic responses, since their platelet counts decreased to less than 600,000/microl. The median platelet count after reduction was 343,000/microl. The median dosage required to achieve these responses and to maintain them for a period of at least four weeks was 1.9 mg/day. Thrombohemorrhagic complications disappeared or did not recur in all symptomatic patients. Adverse effects were seen in 3/12 patients: headache (1), tachycardia (1), palpitation (1) and fluid retention (1). Whereas these symptoms were mild and transitory they caused one patient to request discontinuation of treatment. Currently five patients are still on treatment with anagrelide (median duration of treatment 11 months) while therapy had to be discontinued in the seven others because of bone marrow transplantation, development of osteomyelofibrosis, blast crisis or on patient request. In our experience anagrelide is a useful therapeutic adjunct when thrombocytosis in patients with CML cannot properly controlled alone with traditional drugs. CC Reprint request to: Petro E.Petrides, M.D., Department of CC Medicine III, University of Munich Medical School Grosshadern, CC 15 Marchioninistrasse, 81377 Munich, Germany, CC phone: 49 89 70953133, fax: 49 89 70958137, CC email: petro.petrides@med3.med.uni-muenchen.de AU Broxmeyer-HE. Orazi-A. Hague-NL. Sledge-GWJr. Rasmussen-H. Gordon-MS. TI Myeloid Progenitor Cell Proliferation and Mobilization Effects of BB10010, a Genetically Engineered Variant of Human Macrophage Inflammatory Protein-1alpha, in a Phase I Clinical Trial in Patients with Relapsed/Refractory Breast Cancer SO Blood-Cells-Mol-Dis. 1998 Jan 31. 24(2). P. 14-30. DE chemokine DE MIP-1alpha DE BB10010 DE myelopoiesis DE hematopoietic progenitor cells DE cell cycle DE clinical trial AB Macrophage Inflammatory Protein (MIP)-1alpha is myelosuppressive in vitro and in vivo for hematopoietic stem and immature subsets of myeloid progenitor cells, demonstrates some myeloprotective effects in mice treated with Ara-C and hydroxyurea, and has stem/progenitor cell mobilizing activity in mice. Based on these observations, BB10010, a genetic variant of MIP-1alpha, was assessed for effects on marrow and blood myeloid progenitor cells in patients with relapsed/refractory breast cancer. MIP-1alpha readily polymerizes, whereas BB10010 has a reduced tendency to form large polymers at physiological pH and ionic strength and retains biological activity. Patients were injected with 5, 10, 30 or 100 microg/kg BB10010 s.c. daily for 3 days. BB10010 significantly reduced the cycling status of marrow myeloid progenitors from pretreatment levels of 39-58% to 0 - 11% one day after the third and last injection of BB10010. This was associated with significant decreases in frequency of marrow progenitors (number of colonies formed per number of cells plated) and percent biopsied marrow CD34+ cells. The suppressive effects were reversible in patients and the rapidity of this reversal demonstrated in mouse studies. BB10010 had no effect on nucleated cellularity or on the proliferation of nucleated cells as assessed in marrow biopsies from the patients. These latter effects may in part reflect the noted decreased apoptosis of nucleated cells by BB10010. BB10010 also demonstrated significant but modest myeloid progenitor cell mobilizing capacity. Blood progenitors were in a slow or non-cycling state prior to treatment and this did not change after administration of BB10010. The above effects of BB10010 were similar at the four different dosage levels assessed. These results demonstrate in humans the suppressive and mobilizing effects of MIP-1alpha and BB10010 previously noted in vivo in mice. CC Reprint request to: Hal E. Broxmeyer, Ph.D., Walther CC Oncology Center, Indiana University School of Medicine, CC 1044 West Walnut Street, Indianapolis, IN 46202-5121, CC phone (317)274-7510, fax (317)274-7592, CC email: hal_broxmeyer@iucc.iupui.edu From owner-contents@net.bio.net Wed Feb 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Aquatic Microbial Ecology, vol. 14, no. 02, 13 February 1998 Date: 5 Feb 1998 11:19:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 86 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6bd3b7$qoi@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC AME 14(2) CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Aquatic Microbial Ecology (ISSN 0948-3055) CC Volume 14, Number 2 (1998) CC February 13 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Noble-R-T. Fuhrman-J-A. TI Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 113-118. AU Vadstein-O. TI Evaluation of competitive ability of two heterotrophic planktonic bacteria under phosphorus limitation. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 119-127. AU Donachie-S-P. Zdanowski-M-K. TI Potential digestive function of bacteria in krill Euphausia superba stomachs. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 129-136. AU Pakulski-J-D. Aas-P. Jeffrey-W. Lyons-M. Von-Waasenbergen-L. Mitchell-D. Coffin-R. TI Influence of light on bacterioplankton production and respiration in a subtropical coral reef. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 137-148. AU Vosjan-J-H. van-Noort-G-J. TI Enumerating nucleoid-visible marine bacterioplankton: bacterial abundance determined after storage of formalin fixed samples agrees with isopropanol rinsing method. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 149-154. AU Tillmann-U. TI Phagotrophy by a plastidic haptophyte, Prymnesium patelliferum. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 155-160. AU Hadas-O. Berman-T. TI Seasonal abundance and vertical distribution of Protozoa (flagellates, ciliates), and bacteria in Lake Kinneret, Israel. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 161-170. AU Kuehn-K-A. Suberkropp-K. TI Diel fluctuations in rates of CO2 evolution from standing dead leaf litter of the emergent macrophyte Juncus effusus. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 171-182. AU Piker-L. Schmaljohann-R. Imhoff-J-F. TI Dissimilatory sulfate reduction and methane production in Gotland Deep sediments (Baltic Sea) during a transition period from oxic to anoxic bottom water (1993-1996). SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 183-193. AU Hansen-L-B. Finster-K. Fossing-H. Iversen-N. TI Anaerobic methane oxidation in sulfate depleted sediments: effects of sulfate and molybdate additions. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 195-204. AU Lienemann-C-P. Heissenberger-A. Leppard-G-G. Perret-D. TI Optimal preparation of water samples for the examination of colloidal material by transmission electron microscopy. SO Aquat-Microb-Ecol. 1998 Feb 13. 14(2). P 205-213. From owner-contents@net.bio.net Sun Feb 08 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Marine Ecology Progress Series, vol. 162, no. x, 12 February 1998 Date: 9 Feb 1998 11:35:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 165 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6bnlpn$4s0@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC MEPS 162 CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Marine Ecology Progress Series (ISSN 0171-8630) CC Volume 162 (1998) CC February 12 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Kvitek-R-G. Conlan-K-E. Iampietro-P-J. TI Black pools of death: hypoxic, brine-filled ice gouge depressions become lethal traps for benthic organisms in a shallow Arctic embayment. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 1-10. AU Schell-D-M. Barnett-B-A. Vinette-K-A. TI Carbon and nitrogen isotope ratios in zooplankton of the Bering, Chukchi and Beaufort seas. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 11-23. AU Wood-A-M. Phinney-D-A. Yentsch-C-S. TI Water column transparency and the distribution of spectrally distinct forms of phycoerythrin-containing organisms. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 25-31. AU Boon-A-R. Duineveld-G-C-A. TI Chlorophyll a as a marker for bioturbation and carbon flux in southern and central North Sea sediments. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 33-43. AU Kromkamp-J. Barranguet-C. Peene-J. TI Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 45-55. AU Charpy-L. Blanchot-J. TI Photosynthetic picoplankton in French Polynesian atoll lagoons: estimation of taxa contribution to biomass and production by flow cytometry. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 57-70. AU Chapman-M-G. TI Relationships between spatial patterns of benthic assemblages in a mangrove forest using different levels of taxonomic resolution. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 71-78. AU de-Nys-R. Dworjanyn-S-A. Steinberg-P-D. TI A new method for determining surface concentrations of marine natural products on seaweeds. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 79-87. AU Coma-R. Ribes-M. Gili-J-M. Zabala-M. TI An energetic approach to the study of life-history traits of two modular colonial benthic invertebrates. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 89-103. AU Naganuma-T. Takasugi-H. Kimura-H. TI Abundance of thraustochytrids in coastal plankton. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 105-110. AU Lasker-H-R. Kim-K. Coffroth-M-A. TI Production, settlement, and survival of plexaurid gorgonian recruits. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 111-123. AU Mouneyrac-C. Amiard-J-C. Amiard-Triquet-C. TI Effects of natural factors (salinity and body weight) on cadmium, copper, zinc and metallothionein-like protein levels in resident populations of oysters Crassostrea gigas from a polluted estuary. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 125-135. AU Crooks-J-A. TI Habitat alteration and community-level effects of an exotic mussel, Musculista senhousia. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 137-152. AU Arsenault-D-J. Himmelman-J-H. TI Size-related decrease in spatial refuge use by Iceland scallops Chlamys islandica: ontogenetic behavioural changes or decreasing refuge availability? SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 153-161. AU Heipel-D-A. Bishop-J-D-D. Brand-A-R. Thorpe-J-P. TI Population genetic differentiation of the great scallop Pecten maximus in western Britain investigated by randomly amplified polymorphic DNA. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 163-171. AU Wagner-M. Durbin-E. Buckley-L. TI RNA:DNA ratios as indicators of nutritional condition in the copepod Calanus finmarchicus. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 173-181. AU Lindley-J-A. George-C-L. Evans-S-V. Donkin-P. TI Viability of calanoid copepod eggs from intertidal sediments: a comparison of three estuaries. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 183-190. AU Takahashi-K. Kawaguchi-K. TI Diet and feeding rhythm of the sand-burrowing mysids Archaeomysis kokuboi and A. japonica in Otsuchi Bay, northeastern Japan. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 191-199. AU Haywood-M-D-E. Heales-D-S. Kenyon-R-A. Loneragan-N-R. Vance-D-J. TI Predation of juvenile tiger prawns in a tropical Australian estuary. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 201-214. AU Palma-A-T. Wahle-R-A. Steneck-R-S. TI Different early post-settlement strategies between American lobsters Homarus americanus and rock crabs Cancer irroratus in the Gulf of Maine. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 215-225. AU Tuck-I-D. Hall-S-J. Robertson-M-R. Armstrong-E. Basford-D-J. TI Effects of physical trawling disturbance in a previously unfished sheltered Scottish sea loch. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 227-242. AU Karakassis-I. Tsapakis-M. Hatziyanni-E. TI Seasonal variability in sediment profiles beneath fish farm cages in the Mediterranean. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 243-252. AU Zeller-D-C. TI Spawning aggregations: patterns of movement of the coral trout Plectropomus leopardus (Serranidae) as determined by ultrasonic telemetry. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 253-263. AU Fromentin-J-M. Stenseth-N-C. Gjosaeter-J. Johannessen-T. Planque-B. TI Long-term fluctuations in cod and pollack along the Norwegian Skagerrak coast. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 265-278. AU Culik-B-M. Luna-Jorquera-G. Oyarzo-H. Correa-H. TI Humboldt penguins monitored via VHF telemetry. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 279-286. CC REVIEW AU Best-P-B. Findlay-K-P. Sekiguchi-K. Peddemors-V-M. Rakotonirina-B. Rossouw-A. Gove-D. TI Winter distribution and possible migration routes of humpback whales Megaptera novaeangliae in the southwest Indian Ocean. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 287-299. CC NOTE AU Bak-R-P-M. Meesters-E-H. TI Coral population structure: the hidden information of colony size-frequency distributions. SO Mar-Ecol-Prog-Ser. 1998 Feb 12. 162. P 301-306. From owner-contents@net.bio.net Wed Feb 11 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Bioelectromagnetics, vol. 19, no. 02, 1998 Date: 12 Feb 1998 13:00:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 118 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6bvntp$m2p@net.bio.net> NNTP-Posting-Host: net.bio.net CC BIOELECTROMAGNETICS VOLUME 19, No. 2, 1998 CC Table of Contents CC ======================================================================== CC NOTE: Please contact your library or the authors for reprints or further CC information about specific articles, NOT the editor, Society, or CC Publisher! CC CC For information about the journal, contact the Editor. For member CC subscription information, contact the Society at 7519 Ridge Road, CC Frederick, MD 21702-3519. For library or other non-member subscription CC information, contact the Publisher, Wiley/Liss Inc., 605 Third Avenue, CC New York, NY 10158-0012, Attn.: Subscription Dept., 9th Floor. CC CC Table of Contents from recent previous issues are available on the CC Bioelectromagnetics Society Home Page (http://biomed.ucr.edu/bems.htm) or CC the Publishers' Home Page for this journal CC (http://journals.wiley.com/0197-8462/). CC CC Ben Greenebaum, Editor CC University of Wisconsin-Parkside, Box 2000, Kenosha, WI 53141-2000 CC Internet: bems@uwp.edu CC =========================================================================== CC Bioelectromagnetics CC CC Journal of Bioelectromagnetics Society, the Society for Physical CC Regulation in Biology and Medicine, and the European Bioelectromagnetics CC Association CC CC Volume 19, Number 2, 1998 CC (c) Wiley-Liss, Inc. CC Editor's Note AU Greenebaum-B TI Talking to Each Other SO Bioelectromagnetics, 1998. 19(2) P 67. CC Articles AU Cheng-K. Goldman-R.J. TI Electric Fields and Proliferation in a Dermal Wound Model: Cell Cycle Kinetics SO Bioelectromagnetics, 1998. 19(2). P 68-74. AU Tabrah-F.L. Ross-P. Hoffmeier-M Gilbert,Jr-F. TI Clinical Report on Long-Term Bone Density After Short-Term EMF Application SO Bioelectromagnetics, 1998. 19(2). P 75-78. AU Sienkiewicz-Z.J. Haylock-R.G.E. Saunders-R.D. TI Deficits in Spatial Learning After Exposure of Mice to a 50Hz Magnetic Field SO Bioelectromagnetics, 1998. 19(2). P 79-84. AU Simko-M. Kriehuber-R. Weiss-D.G. Luben-R.A. TI Effects of 50Hz EMF Exposure on Micronucleus Formation and Apoptosis in Transformed and Nontransformed Human Cell Lines SO Bioelectromagnetics, 1998. 19(2). P 85-91. AU Bruner-L.J. Harvey-J.R. TI Synchronization of Pacemaker Cell Firing by Weak ELF Fields: Simulation by a Circuit Model SO Bioelectromagnetics, 1998. 19(2). P 92-97. AU Sastre-A. Cook-M.R. Graham-C. TI Nocturnal Exposure to Intermittent 60 Hz Magnetic Fields Alters Human Cardiac Rhythm SO Bioelectromagnetics, 1998. 19(2). P 98-106. AU Volpe-P. Parasassi-T. Esposito-C. Ravagnan-G. Giusti-A.M. Pasquarelli-A. Eremenko-T. TI Cell Membrane Lipid Molecular Dynamics in a Solenoid Versus a Magnetically Shielded Room SO Bioelectromagnetics, 1998. 19(2). P 107-111. AU Lagroye-I. Poncy-J.L. TI Influence of 50-Hz Magnetic Fields and ionizing Radiation on c-jun and c-fos Oncoproteins SO Bioelectromagnetics, 1998. 19(2). P 112-116. AU Lai-H. Carino-M.A. Ushijima-I. TI Acute Exposure to a 60 Hz Magnetic Field Affects Rats' Water-Maze Performance SO Bioelectromagnetics, 1998. 19(2). P 117-122. AU Rosen-L.A. Barber-I. Lyle-D.B. TI A 0.5 G, 60Hz Magnetic Field Suppresses Melatonin Production in Pinealocytes SO Bioelectromagnetics, 1998. 19(2). P 123-127. CC Brief Communications AU Pakhomova-O.N. Belt-M.L. Mathur-S.P. Lee-J.C. Akyel-Y. TI Ultra-Wide Band Electromagnetic Radiation Does Not Affect UV-Induced Recombination and Mutagenesis in Yeast SO Bioelectromagnetics, 1998. 19(2). P 128-130. AU Ismael-S.J. Callera-F. Garcia-A.B. Baffa-O. Falcao-R.P. TI Increased Dexamethasone-Induced Apoptosis of Thymocytes from Mice Exposed to Long-Term Extremely Low Frequency Magnetic Fields SO Bioelectromagnetics, 1998. 19(2). P 131-135. CC Comments AU Adair-R. TI Extremely Low Frequency Electromagnetic Fields Do Not Interact Directly with DNA SO Bioelectromagnetics, 1998. 19(2). P 136-137. AU Blank-M. Goodman-R. TI Reply to R.K. Adair SO Bioelectromagnetics, 1998. 19(2). P 138. From owner-contents@net.bio.net Sun Feb 15 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Blood Cells, Molecules, & Diseases, vol. 24, no. 03, 15 February 1998 Date: 16 Feb 1998 12:41:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 102 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6ca891$88p@net.bio.net> NNTP-Posting-Host: net.bio.net AU Pellegrino-CM. Rybicki-AC. Musto-S. Nagel-RL. Schwartz-RS. TI Molecular Identification and Expression of Erythroid K:Cl Cotransporter in Human and Mouse Erythroleukemic Cells SO Blood-Cells-Mol-Dis. 1998 Feb 15. 24(3). P. 31-40. DE erythrocyte DE cation transport DE cell volume DE erythropoiesis DE K562 DE MEL AB A major pathway for K+ efflux in human reticulocytes and young RBCs is K:Cl cotransport (K:Cl-CT). The activity of K:Cl-CT is increased in pathologic RBCs containing hemoglobins S and C and may contribute to the abnormal dehydration state of these cells. Human K:Cl-CT (gene product KCC1) has been recently sequenced from human (hKCC1), rabbit and rat tissue by Gillen et al. (J Biol Chem 271:16237, 1996). We report here the sequence of KCC1 from human and mouse erythroleukemic cells (K562 and MEL cells, respectively). The cDNA for human erythroid-KCC1 is 100% identical to hKCC1 and the cDNA for mouse erythroid-KCC1 shares 89% identity with hKCC1, which translates to 96% identity at the amino acid level. Mammalian KCC1 is strongly conserved with >95% identity between human, rabbit, rat, and mouse KCC1 proteins. We did not detect any full-length mRNA transcripts of human erythroid-KCC1 in circulating reticulocytes. We detected two mRNA isoforms of human erythroid-KCC1 that resulted in C-terminal truncated proteins (73 amino acid and 17 amino acids,respectively). Human and mouse erythroid-KCC1 differed at several consensus sites including a predicted PKC phosphorylation site at (108)threonine and a predicted CK2 phosphorylation site at (51)serine,within the predicted cytoplasmic N-terminal, that are present in human but not mouse erythroid-KCC1. Expression of MEL-KCC1 mRNA increases substantially upon DMSO-induced differentiation opening the possibility that erythroid-KCC1 plays a role in early erythroid maturation events. The molecular identification of erythroid-KCC1 is an important step towards understanding the physiologic role mediated by this protein in young and pathologic RBCs and during erythropoiesis, as well as providing a new tool for the elucidation of pathways and signals involved in RBC volume regulation. CC Reprint request to: Robert S. Schwartz, Ph.D., Associate CC Professor of Medicine, Division of Hematology, Albert CC Einstein College of Medicine-Montefiore Medical Center, CC 111 East 210th Street, Bronx, NY 10467-22490, CC phone (718)920-5135, fax (718)881-7108, CC email: rschwart@worldnet.att.net AU Yin-X. Dailey-HA. TI Erythroid 5-Aminolevulinate Synthase is Required for Erythroid Differentiation in Mouse Embryonic Stem Cells SO Blood-Cells-Mol-Dis. 1998 Feb 15. 24(3). P. 41-53. DE heme biosynthesis DE ALA synthase DE erythropoiesis DE regulation AB We have examined the induction of the enzymes of the heme biosynthetic pathway during erythroid differentiation of mouse embryonic stem (ES) cells. Following transfer to appropriate medium all of the pathway enzymes are induced within three days. Unlike differentiating mouse erythroleukemia cells (Lake-Bullock, H. and Dailey, H.A. Mol Cell Biol 13:7122-7132,1993), all of the enzymes appear to be induced simultaneously and not sequentially in differentiating ES cells. The role of erythroid 5-aminolevulinate synthase (ALAS-2) in this differentiation process was examined by disruption of the ALAS-2 gene. The targeting vector used for disruption replaced all of exons 4 to 6 with a selectable neomycin resistance gene. The resulting genetically modified (ALAS-2 knockout) cells, as well as normal ES cells were used to study induction of heme biosynthesis. Following 10 days of culture in methylcellulose media significant morphological differences between the embryoid bodies (EBs) of the two cell lines were observed. ES cells exhibited morphology of typical EBs with a dark field (blood island) in the center, while ALAS-2 knockout ES cells developed very poorly both in size and shape. At 8 days of differentiation, only 3% of all EBs contained visible erythropoietic cells (i.e., stained positively for hemoglobin) in the ALAS-2 knockout cell line, compared with 50% in ES cells. Most of the genes in the heme synthetic pathway were expressed to a stable level within 3 to 6 days after induction in normal ES cells, while the ALAS-2 knockout cell line failed to significantly increase the level of expression of these genes. Fetal beta-globin mRNA was not detectable in the differentiating ALAS-2 knockout cells, whereas mRNA for this gene was detected in normal ES cells within 3 days of differentiation. These results suggest that ALAS-2 is necessary for ES cell erythroid differentiation and that there is an interrelationship between heme and globin synthesis in differentiating ES cells. CC Reprint request to: Harry A. Dailey, Ph.D., Department of CC Biochemistry and Molecular Biology, University of Georgia, CC Athens GA 30602-7229, phone (706) 542-2690, CC fax (706)542-7567, email: dailey@bscr.uga.edu From owner-contents@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Diseases of Aquatic Organisms, vol. 32, no. 01, 26 February 1998 Date: 19 Feb 1998 10:13:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6chsno$88p@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC DAO 32(1) CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Diseases of Aquatic Organisms (ISSN 0177-5103) CC Volume 32, Number 1 (1998) CC February 26 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Zupanovic-Z. Lopez-G. Hyatt-A-D. Green-B. Bartran-G. Parkes-H. Whittington-R-J. Speare-R. TI Giant toads Bufo marinus in Australia and Venezuela have antibodies against 'ranaviruses'. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 1-8. AU Holzschu-D-L. Wooster-G-A. Bowser-P-R. TI Experimental transmission of dermal sarcoma to the sauger Stizostedion canadense. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 9-14. AU Marty-G-D. Freiberg-E-F. Meyers-T-R. Wilcock-J. Farver-T-B. Hinton-D-E. TI Viral hemorrhagic septicemia virus, Ichthyophonus hoferi, and other causes of morbidity in Pacific herring Clupea pallasi spawning in Prince William Sound, Alaska, USA. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 15-40. AU Feng-S. Woo-P-T-K. TI Characterization of a 200 kDa glycoprotein (Cs-gp200) on the pathogenic piscine haemoflagellate Cryptobia salmositica. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 41-48. CC REVIEW AU Wiklund-T. Dalsgaard-I. TI Occurrence and significance of atypical Aeromonas salmonicida in non-salmonid and salmonid fish species: a review. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 49-69. CC NOTES AU Abollo-E. Lopez-A. Gestal-C. Benavente-P. Pascual-S. TI Long-term recording of gastric ulcers in cetaceans stranded on the Galician (NW Spain) coast. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 71-73. AU Arias-C. Gestal-C. Rodriguez-H. Soto-M. Estevez-J. TI Palaemon elegans, an intermediate host in the life-cycle of Aggregata octopiana. SO Dis-Aquat-Org. 1998 Feb 26. 32(1). P 75-78.