From owner-contents@net.bio.net Thu Aug 06 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Scott Hanson Newsgroups: bionet.journals.contents Subject: Diseases of Aquatic Organisms, vol. 33, no. 03, 30 July 1998 Date: 7 Aug 1998 16:43:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC DAO 33(3) CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Diseases of Aquatic Organisms (ISSN 0177-5103) CC Volume 33, Number 3 (1998) CC July 30 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Palm-R-C-Jr. Landolt-M-L. Busch-R-A. TI Route of vaccine administration: effects on the specific humoral response in rainbow trout Oncorhynchus mykiss. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 157-166. AU Chakroun-C. Grimont-F. Urdaci-M-C. Bernardet-J-F. TI Fingerprinting of Flavobacterium psychrophilum isolates by ribotyping and plasmid profiling. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 167-177. AU Dawson-L-H-J. Pike-A-W. Houlihan-D-F. McVicar-A-H. TI Effects of salmon lice Lepeophtheirus salmonis on sea trout Salmo trutta at different times after seawater transfer. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 179-186. AU Tojo-J-L. Santamarina-M-T. TI Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. II: Gyrodactylus sp. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 187-193. AU Tojo-J-L. Santamarina-M-T. TI Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. III: Ichthyobodo necator. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 195-199. AU Rantanen-J-T. Valtonen-E-T. Holopainen-I-J. TI Digenean parasites of the bivalve mollusc Pisidium amnicum in a small river in eastern Finland. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 201-208. AU Ragone-Calvo-L-M. Walker-J-G. Burreson-E-M. TI Prevalence and distribution of QPX, Quahog Parasite Unknown, in hard clams Mercenaria mercenaria in Virginia, USA. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 209-219. AU Nadala-E-C-B-Jr. Tapay-L-M. Loh-P-C. TI Characterization of a non-occluded baculovirus-like agent pathogenic to penaeid shrimp. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 221-229. CC NOTES AU Nadala-E-C-B-Jr. Loh-P-C. TI A comparative study of three different isolates of white spot virus. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 231-234. AU Usheva-L-N. Odintsova-N-A. TI Hyperplastic growth of mucous cells in the mantle of the mussel Modiolus kurilensis from a heavily polluted area of Amursky Bay, Sea of Japan. SO Dis-Aquat-Org. 1998 Jul 30. 33(3). P 235-238. From owner-contents@net.bio.net Thu Aug 06 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Scott Hanson Newsgroups: bionet.journals.contents Subject: Marine Ecology Progress Series, vol. 169, no. x, 6 August 1998 Date: 7 Aug 1998 16:45:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 167 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC MEPS 169 CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Marine Ecology Progress Series (ISSN 0171-8630) CC Volume 169 (1998) CC August 6 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Uthicke-S. Klumpp-D-W. TI Microphytobenthos community production at a near-shore coral reef: seasonal variation and response to ammonium recycled by holothurians. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 1-11. AU Flynn-K-J. TI Estimation of kinetic parameters for the transport of nitrate and ammonium into marine phytoplankton. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 13-28. AU Waser-N-A. Yin-K. Yu-Z. Tada-K. Harrison-P-J. Turpin-D-H. Calvert-S-E. TI Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 29-41. AU Lizon-F. Seuront-L. Lagadeuc-Y. TI Photoadaptation and primary production study in tidally mixed coastal waters using a Lagrangian model. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 43-54. AU Lavaniegos-B-E. Gomez-Gutierrez-J. Lara-Lara-J-R. Hernandez-Vazquez-S. TI Long-term changes in zooplankton volumes in the California Current System--the Baja California region. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 55-64. AU Bargagli-R. Monaci-F. Sanchez-Hernandez-J-C. Cateni-D. TI Biomagnification of mercury in an Antarctic marine coastal food web. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 65-76. AU Moodley-L. Schaub-B-E-M. van-der-Zwaan-G-J. Herman-P-M-J. TI Tolerance of benthic foraminifera (Protista: Sarcodina) to hydrogen sulphide. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 77-86. AU Kelman-D. Kushmaro-A. Loya-Y. Kashman-Y. Benayahu-Y. TI Antimicrobial activity of a Red Sea soft coral, Parerythropodium fulvum fulvum: reproductive and developmental considerations. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 87-95. AU Goericke-R. Montoya-J-P. TI Estimating the contribution of microalgal taxa to chlorophyll a in the field--variations of pigment ratios under nutrient- and light-limited growth. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 97-112. AU Moisan-T-A. Olaizola-M. Mitchell-B-G. TI Xanthophyll cycling in Phaeocystis antarctica: changes in cellular fluorescence. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 113-121. AU Townsend-E-C. Fonseca-M-S. TI Bioturbation as a potential mechanism influencing spatial heterogeneity of North Carolina seagrass beds. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 123-132. AU Procaccini-G. Mazzella-L. TI Population genetic structure and gene flow in the seagrass Posidonia oceanica assessed using microsatellite analysis. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 133-141. AU Taylor-R-B. Peek-J-T-A. Rees-T-A-V. TI Scaling of ammonium uptake by seaweeds to surface area:volume ratio:geographical variation and the role of uptake by passive diffusion. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 143-148. AU Calvo-Ugarteburu-G. McQuaid-C-D. TI Parasitism and invasive species: effects of digenetic trematodes on mussels. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 149-163. AU Beck-M-W. TI Comparison of the measurement and effects of habitat structure on gastropods in rocky intertidal and mangrove habitats. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 165-178. AU Johnson-M-P. Burrows-M-T. Hawkins-S-J. TI Individual based simulations of the direct and indirect effects of limpets on a rocky shore Fucus mosaic. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 179-188. AU Lynch-D-R. Gentleman-W-C. McGillicuddy-D-J-Jr. Davis-C-S. TI Biological/physical simulations of Calanus finmarchicus population dynamics in the Gulf of Maine. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 189-210. AU Melle-W. Skjoldal-H-R. TI Reproduction and development of Calanus finmarchicus, C. glacialis and C. hyperboreus in the Barents Sea. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 211-228. AU Metz-C. TI Feeding of Oncaea curvata (Poecilostomatoida, Copepoda). SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 229-235. AU Sulkin-S. Lehto-J. Strom-S. Hutchinson-D. TI Nutritional role of protists in the diet of first stage larvae of the Dungeness crab Cancer magister. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 237-242. AU Pinn-E-H. James-R. Atkinson-A. Rogerson-A. TI Particle size selectivity and resource partitioning in five species of Thalassinidea (Crustacea: Decapoda). SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 243-250. AU Connell-S-D. TI Effects of predators on growth, mortality and abundance of a juvenile reef-fish: evidence from manipulations of predator and prey abundance. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 251-261. AU Trathan-P-N. Murphy-E-J. Croxall-J-P. Everson-I. TI Use of at-sea distribution data to derive potential foraging ranges of macaroni penguins during the breeding season. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 263-275. CC NOTES AU Wunsch-M. Richter-C. TI The CaveCam--an endoscopic underwater videosystem for the exploration of cryptic habitats. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 277-282. AU Pedersen-O. Borum-J. Duarte-C-M. Fortes-M-D. TI Oxygen dynamics in the rhizosphere of Cymodocea rotundata. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 283-288. AU Noda-T. Fukushima-K. Mori-T. TI Daily settlement variability of the barnacle Semibalanus cariosus: importance of physical factors and density-dependent processes. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 289-293. AU Mokady-O. Loya-Y. Lazar-B. TI Ammonium contribution from boring bivalves to their coral host--a mutualistic symbiosis? SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 295-301. AU Gaspar-M-B. Castro-M. Monteiro-C-C. TI Influence of tow duration and tooth length on the number of damaged razor clams Ensis siliqua. SO Mar-Ecol-Prog-Ser. 1998 Aug 6. 169. P 303-305. From owner-contents@net.bio.net Fri Aug 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Biosci Administrator Newsgroups: bionet.journals.contents Subject: Aquatic Microbial Ecology, vol. 15, no. 3, 31 August 1998 Date: 15 Aug 1998 14:45:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 84 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6r4vhs$ada@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC AME 15(3) CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Aquatic Microbial Ecology (ISSN 0948-3055) CC Volume 15, Number 3 (1998) CC August 31 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Nagasaki-K. Yamaguchi-M. TI Effect of temperature on the algicidal activity and the stability of HaV (Heterosigma akashiwo virus). SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 211-216. AU Crottereau-C. Delmas-D. TI Exoproteolytic activity in an Atlantic pond (France): estimates of in situ activity. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 217-224. AU Francis-C-A. Francis-A-K. Golet-D-S. Ward-B-B. TI Quantification of catechol 2,3-dioxygenase gene homology and benzoate utilization in intertidal sediments. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 225-231. AU Maximilien-R. de-Nys-R. Holmstroem-C. Gram-L. Givskov-M. Crass-K. Kjelleberg-S. Steinberg-P-D. TI Chemical mediation of bacterial surface colonisation by secondary metabolites from the red alga Delisea pulchra. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 233-246. AU Guerrini-F. Mazzotti-A. Boni-L. Pistocchi-R. TI Bacterial-algal interactions in polysaccharide production. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 247-253. AU Kormas-K-A. Kapiris-K. Thessalou-Legaki-M. Nicolaidou-A. TI Quantitative relationships between phytoplankton, bacteria and protists in an Aegean semi-enclosed embayment (Maliakos Gulf, Greece). SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 255-264. AU Letelier-R-M. Karl-D-M. TI Trichodesmium spp. physiology and nutrient fluxes in the North Pacific subtropical gyre. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 265-276. AU Burford-M-A. Pearson-D-C. TI Effect of different nitrogen sources on phytoplankton composition in aquaculture ponds. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 277-284. AU Weisse-T. Montagnes-D-J-S. TI Effect of temperature on inter- and intraspecific isolates of Urotricha (Prostomatida, Ciliophora). SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 285-291. AU Skovgaard-A. TI Role of chloroplast retention in a marine dinoflagellate. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 293-301. AU Christian-J-R. Karl-D-M. TI Ectoaminopeptidase specificity and regulation in Antarctic marine pelagic microbial communities. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 303-310. CC NOTE AU Blaabjerg-V. Finster-K. TI Sulphate reduction associated with roots and rhizomes of the marine macrophyte Zostera marina. SO Aquat-Microb-Ecol. 1998 Aug 31. 15(3). P 311-314. From owner-contents@net.bio.net Sun Aug 23 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Biotechnologia Aplicada, vol. 15, no. 02, Apr-Jun 1998 Date: 24 Aug 1998 12:40:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 506 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6rsfji$4kg@net.bio.net> NNTP-Posting-Host: net.bio.net BIOTECNOLOGIA APLICADA CC Vol. 15 No. 2 (April-June, 1998) CC ISSN 0864-4551 (printed) CC ISSN 1027-2852 (electronic) CC Copyright 1998, Elfos Scientiae. CC Founded in 1983 and published as Interferón y CC Biotecnología until 1990, Biotecnología Aplicada is a CC peer-reviewed scientific journal. It appears four times CC per year, sponsored by the Ibero-Latin-American Society CC of Biotechnology Applied to Health. CC BIOTECNOLOGIA APLICADA is a vehicle of Iberian-Latin- CC American scientists working in different fields of modern CC biotechnology for the diffusion of their experiences and CC results. Some of the main topics that are considered for CC publication are the following: CC a) Molecular genetics and recombinant DNA technology; CC b) Therapeutic and prophylactic uses of molecules CC obtained by biotechnological methods; CC c) Immunotechnology and development of diagnostic CC methods; CC d) Chemistry, biochemistry and design of molecules with CC biological activity; CC e) Application of recombinant DNA techniques and its CC products in agriculture and animal science; CC f) Transgenesis; CC g) Fermentation, recovery and purification of biotech- CC nological products; CC h) Scaling-up, plant design and economic evaluation; CC i) Control and automation of processes. CC All rights reserved. No part of this publication may be CC reproduced, stored or transmitted in any form or by any CC means without the prior permission of the Publisher. By CC submitting a manuscript the authors agree that the CC copyright for their article is transferred to the CC Publisher if and when the article is accepted for CC publication. The points of view expressed in the Journal CC are exclusive responsibility of the authors. CC This journal is indexed by the following publications: CC Biosis, Current Biotechnology Abstracts, Derwent Biotech- CC nology Abstracts, Cambridge Scientific Abstracts, CC Chemical Abstracts, Biological Abstracts, EMBASE, CC Elsevier BIOBASE, Index Medicus, LilaCS, CC BioJournals (Internet). CC Correspondence and subscriptions CC Ave. 31 entre 158 y 190, CC Cubanacán, Playa CC Ciudad de La Habana, CC Apdo. 6072, Habana 6, Cuba. CC Telephones: (53-7) 33 1917 / 21-8466 / 21-8164 CC Fax: (53-7) 33 1917 / 21-8070 / 33-6008 CC E-mail: elfos@cigb.edu.cu CC Full papers are available online through BIOLINE System CC (Internet) through the WWW or by e-mail. Annual subscrip- CC tion or single document purchase are possible. Contact CC the following for further information: CC URL:http://www.bd.org.br/bioline/ CC E-mail address: bio@biostrat.demon.co.uk AU Jorge Berlanga, Silvia Álvarez, José de la Fuente and Pedro López-Saura IN Mammalian Cells Genetics Division, Clinical Trials Division. Center for Genetic Engineering and Biotechnology. PO Box 6162, Havana 10600, Cuba. Telef: (53-7) 21 8164; Fax: (53-7) 21 8070; 33 8008; E-mail: jorge.berlanga@cigb.edu.cu TI Considerations on the transforming potential of the epidermal growth factor DE EGF DE carcinogenesis DE malignancy DE toxicitySO Biotecnologia Aplicada 1998;15:65-69 AB The epidermal growth factor (EGF) is a potent mitogen for a variety of epithelial and mesenchyma derived cells. Cells treated with EGF exhibit transient metabolic changes and a morphologic phenotype which mirror those found in malignant counterparts. EGF binds to a specific membrane receptor with intrinsic tyrosine kinase activity coded by a cellular proto-oncogene, whose mutated form has been largely implicated in malignancy. Experimental evidence indicates that EGF potentiates chemical carcinogenesis in vivo as well as viral transformation in vitro. Thus EGF has been historically considered as a tumor promoter. Nevertheless, recent experimental findings, mostly derived from preclinical models, strongly suggest that EGF treatment is insufficient to initiate benign or malignant transformation. On the contrary, in certain scenarios EGF may act as a potent cytoprotective agent for normal cells, and sensitizes cancer cells to anti- tumor therapy. All these experimental data provide fundamental basis for the clinical use of EGF in terms of safety. The clinical experience accumulated SO far suggests that EGF does not seem to initiate carcinogenesis. CC Review Article CC Language: English AU Rolando González-Cabañas, Joel Ferrero-Bibilonia, María G Morales-Menéndez, Angel Aguilera-Rodríguez y Pedro López-Saura IN Centro de Investigaciones Biológicas, apartado postal 6996, La Habana, Cuba. Telef: (53-7) 21 9635; E-mail: CLINTR@cigbdec.cigb.edu.cu; Instituto de Gastroenterología, 25 e I, Vedado, La Habana, Cuba. TI Inmunogenicidad del interferón alfa-2b recombinante (Heberón alfa R). Detección de anticuerpos mediante un ensayo inmunoenzimático y neutralización de actividad antiviral DE antibodies DE anti-interferon alpha-2 DE ELISA DE neutralization SO Biotecnologia Aplicada 1998;15:71-76 AB We studied the presence of anti-interferon antibodies in samples of healthy or sick individuals treated or not with natural leukocyte (Heberón alfa N) or recombinant alpha-2b (Heberón alfa R) interferon (IFN) by means of an immunoenzymatic assay (ELISA), "sandwich"-type (IFN alpha-2b/sample /protein A- peroxidase) with specificity confirmation. The IFN alpha-2b antiviral activity neutralization was alSO investigated. No anti-IFN antibodies were found by ELISA in 184 not treated individuals while among other samples the bioassay detected neutralizing activity in subjects with viral chronic hepatitis (8/181; 4.4 %) and neoplasia (11/121; 9.1 %). Ninety-eight healthy subjects or patients with other illness were negative. There was no positivity in leukocyte IFN-treated individuals, 27 of them evaluated by ELISA and 52 by the bioassay. After the treatment with IFN alpha-2b positivity by ELISA was confirmed in a girl with lymphangioma and in an adult with laryngeal papillomatosis among 177 patients. These ELISA-positive sera had neutralizing titers of 1:25 and 1:64 000 respectively. IFN alpha-2b treatment induced neutralizing activity in 13.1 % (19/145) of the patients evaluated by bioassay. The total incidence of treatment-induced antibodies, evaluated by either method, was 6.4 % (19/296). This is similar to what has been reported for IFN alpha-2b. CC Research Article CC Language: Spanish AU José Raymundo Enríquez del Valle, Guillermo Carrillo- Castañeda y Juan Velázquez Mendoza. IN Colegio de Postgraduados, Montecillo, Méx. 56230, México. TI Producción de vitroplántulas de tomate (Lycopersicon esculentum Mill) y determinación de la producción de fruto DE shoot induction DE micropropagation DE yield SO Biotecnologia Aplicada 1998;15:77-82 AB Appropriate technology to enhance shoot induction ability and vitroplants quality, characteristics determined by the genotype but strongly affected by the environment, might facilitate the generation of transgenic crops. 1. In the tomato variety Contessa 98 % of the leaf explants formed callus but shoot induction: a) was observed in 59.97 % of the explants being 2.4 the mean number of shoots per explant if the back side of the leaf disks were placed in contact with the medium. Half as much was the percentage obtained putting the other side of the explants in contact with the medium; b) was modified by age and period of the year in which the leaf tissue of the donor plant were developed since 95.7, 90.2 and 49.6 % of the explants obtained from 4, 6 and 8 weeks old plants respectively developed shoots being, in the same order, 4.27, 4.32 and 2.61 the mean number of shoots obtained per explant; c) was detected in 98 % of vitroplantlet leaf tissue explants and 3.27 mean shoots per explant were obtained. 2. Similar performance of the plants obtained from seed and from leaf tissue was observed, in particular fruit quality and yields, when all of them were cultured under soil conditions. CC Research Article CC Language: Spanish AU Jorge Berlanga, Jorge Lodos, Osvaldo Reyes, Juan F Infante, Enrique Caballero and Pedro López-Saura IN Center for Genetic Engineering and Biotechnology. PO Box 6162, Havana 10600, Cuba. E-mail: jorge.berlanga@cigb.edu.cu; Department of Pathology, Finlay Institute, Havana, Cuba. TI Epidermal growth factor stimulated re-epithelialization in pigs. The possible role of acute-wound proteases DE wound healing DE EGF DE re-epithelialization SO Biotecnologia Aplicada 1998;15:83-87 AB The epithelial response to a daily topical treatment regime with three different epidermal growth factor (EGF) concentrations was evaluated in five pigs. Animals were kept under controlled environmental conditions, and 30 partial-thickness wounds were inflicted on the dorsum. These were assigned to treatments with 50, 10 and 2 µg of human reacombinant EGF (hr-EGF/g). Control wounds received cream vehicle or were untreated. Wounds were cleansed, inspected and treated every day accordingly. The wound-derived exudate was collected from the untreated wounds during the first 24 h post-injury, and filter-sterilized to qualitatively estimate the presence of protease activity on a fluorescent synthetic peptide by electrophoresis. After 7 EGF treatments, pigs were sacrificed on day 8 and the wounds were harvested and processed to determine the percentage of total re- epithelialization, average radius, and wound perimeter, as the linear ingrowth. The computerized morphometric study proved that only the largest dose significantly stimulated re-epithelialization, whereas the other doses were ineffective. It was alSO shown that the acute phase-wounds exudate exhibits proteolytic activity which completely degraded the fluorescent substrate. This proteolytic activity was similarly inhibited by 0.5 and 5 µg of a Kunitz-type protease inhibitor. Our results lead us to speculate that the bioavailability of topically applied EGF to accelerate wound healing may be reduced by proteases derived from local acute wounds. CC Research Article CC Language: English AU Elaine Santana, Violeta Labarta, Tania González y José de la Fuente IN División de Genética de Células de Mamíferos y División de Estudios Preclínicos y Clínicos, Centro de Ingeniería Genética y Biotecnología. Apartado postal 6162, La Habana, Cuba. Telf.:(53-7) 21 8466, 21 8164; Fax: (53-7) 21 8070, 33 8008; E-mail: transg@cigb.edu.cu TI Relación entre infección por vih y afecciones infecciosas y no infecciosas incluidas dentro del sida DE tuberculosis DE Kaposi's sarcoma DE epidemic neuropathy SO Biotecnologia Aplicada 1998;15:88-91 AB Since the human immunodeficiency virus (HIV) was proposed as the cause of the acquired immune deficiency syndrome (AIDS), many investigations have been conducted to establish the relationship between these two entities. Nevertheless, as alSO described for other viral diseases, the validity of the Koch's postulates has not been established for the HIV. Although most of the researchers consider that the HIV is the cause of AIDS, few argue that no relationship exists between these two entities. AIDS is characterized in most of the infected people by a debilitation of the immune system, followed by infectious and non-infectious diseases which finally result in patients death. Taking advantage of the exceptionally good conditions found in Cuba for the epidemiology and control of the HIV infection, we studied the incidence of tuberculosis (TB), Kaposi's sarcoma (KS) and epidemic neuropathy (EN) in HIV- (N = 11 004 563) and HIV+ (N = 1 303) individuals between 1986 and 1996. The odd ratio was calculated to compare the incidence of these diseases in HIV infected and non-infected people. The results indicated that for the diseases included in the AIDS (infectious: TB, odd > 22 and non-infectious: KS, odd > 3100), the incidence was higher among HIV infected individuals. For the NE, not included in the AIDS, the incidence in HIV non-infected individuals was higher than in HIV+ (odd = 0.49). These results added new evidences showing that for our study conditions, a cause-effect relationship exists between the HIV infection and the AIDS infectious (TB) and non- infectious (KS) diseases. CC Research Article CC Language: Spanish AU Idalmis Reverón, Alberto Agraz y Luis C Pérez IN División de Desarrollo Biofarmacéutico, Departamento de Estudios Clínicos, Centro de Ingeniería Genética y Biotecnología, apartado postal 6162, La Habana, Cuba. Fax: (53-7) 21 8675; E-mail: desarrollo@cigb.edu.cu TI Estudios de extracción del principio activo en cremas de interferón humano (?-2b recombinante y leucocitario) DE extraction DE cream DE quantitation DE recombinant alpha IFN DE leukocyte IFN DE base SO Biotecnologia Aplicada 1998;15:93-97 AB Extraction and quantitation studies of the active ingredient from an hydrophilic (potassium estearate)cream prepared either with recombinant or leukocyte human interferons, were done. The cream samples were treated with different extraction solutions including acids, alkali, surfactants and organic solvents. Recombinant alpha interferon (IFN) was best extracted using acetone (1-5 %) or using a two phase extraction system composed by diethyl ether: H2O (1:1). With this solutions about 80 % of the active ingredient was recovered from the cream. In the case of leukocyte IFN, the two phase extraction system using diethyl ether: H2O (1:1) extracted up to 95 % of the IFN. In addition other cream bases were assayed for the formulation of the IFNs creams. High recovery (99 %) of the active ingredient was obtained using diethyl ether: H2O (1:1) for the following creams: a) Potasium estearate, b) base composed of polysorbate 80 (0.83 %), propylenglycol (10 %), cetilyc alcohol (1.14 %), glyceride monoestearate (6.67 %), methylparabene (0.18 %), propylparabene (0.02 %), H2O: csp, combined with carbopol 940 gel to 0.5 %, and c) the same as b) without carbopol 940. CC Article on Techniques CC Language: Spanish AU Esther M Fajardo, Yilian Plascencia, Adelina Chávez, Xenia Muñoz, Regla Sosa, Eduardo Álvarez e Iyalili Labrador IN Instituto Finlay, Ave. 27 No. 19805, AP 16017, CP 11600, La Lisa, La Habana, Cuba. Tel.: (53-7) 21 4635; Fax: (53-7) 33 6075; E-mail: efajardo@finlay.edu.cu TI Metodología para el establecimiento del ensayo de potencia de la vacuna antipertussis de células completas DE pertussis vaccine DE pertussis DE potency DE OF-1 mouse SO Biotecnologia Aplicada 1998;15:98-101 AB The World Health Organization (WHO) Requirements for the whole-cell pertussis vaccine describe the potency test in a very general manner because it varies from one laboratory to another; one of the reasons for that variation could be the mouse strain used, therefore every laboratory shall evaluate the best conditions for its performance. To be considered valid, the effective dose 50 % (ED50) value shall be between the largest and the smallest immunizing doses and the regression do not show significant deviations from linearity. The optimal dose range is experimentally determined in each laboratory. For the establishment of this assay in our laboratory the OF-1 mouse strain was used. Different immunizing doses were inoculated to get a surviving response which had to fulfil a linear regression analysis for be considered appropriate. Ten assays were performed following the same methodology. Six resulted valid. The logarithmic transformation of the doses used and their survival percent showed a good linear regression (R ? 0.99) in the range of immunizing doses containing 2; 0.4 and 0.08 International Units (IU). Eight lots of Diphtheria-Tetanus-Pertussis (DTP) vaccine were alSO evaluated to prove that the potency test worked well. All the assays were valid according to WHO requirements. The methodology described in the present paper was considered appropriate to evaluate in our laboratory the potency of the whole-cell pertussis component in DTP vaccines. CC Article on Techniques CC Language: Spanish AU Nelson S Vispo, Manuel de J Araña, Glay Chinea, Ariana G Ojalvo and Gianni Cesareni IN Centro de Ingeniería Genética y Biotecnología, Apartado postal 6162, Ciudad de La Habana CP 10600, Cuba; Universitá di Roma Torvergata, Roma, Italy. TI Epitopic mapping of two anti-human Interleukin-2 monoclonal antibodies using a phage displayed-peptide library DE SO Biotecnologia Aplicada 1998;15:103 CC Report CC Language: English AU Bryan R and Williams G IN Department of Cancer Biology Lerner Research Institute, Cleveland Clinic Foundation, Cleveland Ohio, 44195 USA TI New paradigms in interferon and cytokine signaling SO Biotecnologia Aplicada 1998;15:104-105 CC Report CC Language: English AU Silvio E Perea, Omar López-Ocejo, Gustavo Bracho, Rolando García-Milián and Manuel de J Araña IN División de Farmacéuticos, Centro de Ingeniería Genética y Biotecnología, Apartado postal 6162. Ciudad de La Habana CP 10600. Cuba TI Molecular Bases for Human Papillomavirus resistance to Interferon Action SO Biotecnologia Aplicada 1998;15:105 CC Report CC Language: English AU Freya M Freyre, Javier E Vázquez, Marta Ayala, Leonardo Canaán-Haden, Hanssel Bell, Alberto Cintado and Jorge V Gavilondo IN Centro de Ingeniería Genética y Biotecnología, División de Inmunotecnología y Diagnóstico, apartado postal 6162, Ciudad de La Habana, 10600, Cuba. E-mail: Jorge.Gavilondo@cigb.edu.cu TI Cloning and expression of an anti cea single chain antibody fragment in the methylotrophic yeast pichia pastoris SO Biotecnologia Aplicada 1998;15:106 CC Report CC Language: English AU Vélez M, Carrascosa JL, Müller DJ y Engel A IN Laboratorio de Bajas Temperaturas C-III, Departamento Física de la Materia Condensada y Centro Nacional de Biotecnologia, CSIC, Universidad Autónoma de Madrid, E- 28049 Madrid, España; M.E.Müller-lnstitute for Microscopic Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland; Forschungszentrum Jülich, IBI-2: Structural Biology, D-52425 Jülich, Germany. TI Visualización mediante el microscopio de fuerzas atómicas del conector del bacteriófago ? 29 sumergido en solución SO Biotecnologia Aplicada 1998;15:107 CC Report CC Language: English AU Vivian Huerta, Vivian Morera, Nelia López, Lázaro Betancourt, Vladimir Besada, Gabriel Padrón, Gipsi Lima, María de los A Chávez, Julieta Delfín and Joaquín Díaz IN División de Química-Física, Centro de Ingeniería Genética y Biotecnología. Apartado postal 6162, Ciudad de La Habana, CP 10600, Cuba. E-mail: vivian.morera@cigb.edu.cu; Departamento de Bioquímica, Facultad de Biología, Universidad de La Habana, Calle 25 No. 455, Plaza, Ciudad de La Habana, Cuba. TI Characterization and 3D model of a new proteinase inhibitor isolated from STICHODACTYLA HELIANTHUS SO Biotecnologia Aplicada 1998;15:108 CC Report CC Language: English AU Glay Chinea, TirSO Pons, Osvaldo Olmea and Gabriel Márquez IN Division of Physical Chemistry, Center for Genetic Engineering and Biotechnology. PO Box 6162, Havana, Cuba. E-mail: Protein.Design@cigb.edu.cu TI Modeling the flavivirus envelope. Prediction of functional residues by the analysis of E-glycoprotein sequence conservation patterns SO Biotecnologia Aplicada 1998;15:109 CC Report CC Language: English AU Sergio Lizano IN Instituto Clodomiro Picado, Facultad de Microbiología, and Departamento de Bioquímica, Escuela de Medicina, Universidad de Costa Rica, San José, Costa Rica TI Streptokinase-mediated plasminogen activation: molecular studies using genetically engineered streptokinase variants SO Biotecnologia Aplicada 1998;15:110 CC Report CC Language: English AU Mercedes Delgado Fernández IN Instituto Superior Politécnico "José Antonio Echeverría", Centro de Inmunología Molecular, Calle 216 esq. A 15, Atabey, Playa, apartado. postal 16040, Habana 11600, Cuba Tel./Fax: (53-7) 335049, 333509, E- mail: mercedes@ict.cim.sld.cu TI Tendencias en los sistemas de gestión de calidad en la I+D biofarmacéutica DE quality DE R&D DE biopharmaceutical DE quality systems DE quality management SO Biotecnologia Aplicada 1998;15:111-116 AB To establish quality systems from the biopharmaceutical R&D is vital for this industry, in spite of being a thematic area, not very much developed in international level. This paper shows the tendencies in this field of interest, standing out the need and the way to establish a model of quality management which integrates the main factors that make quality and the innovation, since the first periods of the development of the product as a way to solve the current market conditions and the needs of our country. CC Focus CC Language: Spanish AU International Committee of Medical Journal Editors IN Annals of Internal Medicine, BMJ, Canadian Medical Association Journal, Journal of the American Medical Association, Lancet, Medical Journal of Australia, New England Journal of Medicine, New Zealand Medical Journal, Tidsskrift for Den Norske Lægeforening, Western Journal of Medicine, Index Medicus TI Uniform requirements for manuscripts submitted to biomedical journals, and additional statements, 1997 SO Biotecnologia Aplicada 1998;15:117-127 CC Focus CC Language: English