From scimedweb from mail.com Thu Oct 2 07:11:44 2008 From: scimedweb from mail.com (scimedweb@mail.com) Date: Thu Oct 2 09:19:39 2008 Subject: [Cell-biology] An update on tumor suppressor genes in breast cancer (book). Message-ID: <2ca21e2e-da04-43ce-8033-ea2c7fc4f9a4@m36g2000hse.googlegroups.com> Tumor suppressor genes in breast cancer (book). by Marc Lacroix InTextoResearch, Baelen (Wallonia), Belgium Nova Sciences Publishers, New York, ISBN 978-1-60456-326-9 (October 2008) https://www.novapublishers.com/catalog/product_info.php?products_id=3D6866 Breast cancer is characterized by the accumulation of genetic alterations, including point mutations and loss of entire DNA regions (=93loss of heterozygosity=94 or LOH). Among genes that are affected by such events, the =93tumor suppressor genes=94 (TSGs) have a peculiar interest since they often occupy pivotal positions in regulatory networks that control the cell cycle and/or encompass various signal transduction cascades. While a number of genes have been suggested as candidate TSGs in breast cancer, only a few of them have been confirmed in this status. They include TP53, BRCA1, BRCA2=85and are mainly involved in the control of DNA repair, cell proliferation, apoptosis and signaling. Some TSGs are linked to familial (hereditary) forms of breast cancer. The exact definition of what is a TSG is still debated. Recently, genes not affected by mutation or even LOH, but occasionally methylated have been considered as TSGs. Genes discussed: On chromosome 1: CLCA2, DIRAS3 (ARHI, RHOI, NOEY2), LPHH1, TTC4, RAD54L (RAD54), FABP3 (MDGI), RUNX3 (CBFA3, AML2), PRDM2 transcript 1 (RIZ transcript 1), SFN (14-3-3s) On chromosome 3: ROBO1 (DUTT1), PTPG (PTPRG); FHIT (FRA3B, included), PB1 (BAF180), RASSF1 transcript A, RBM5 (LUCA-15, H37), TUSC4 (NPRL2), TMEM158 (RIS1), SEMA3B, TGFBR2 (HNPCC6), APRG1 (C3ORF35), RARB transcript 2, VHL, ATR (FRP1) On chromosome 4: SLIT2, PRDM5, HPGD (PGDH1) On chromosome 5: DAB2 (C9), APC, IRF1 On chromosome 6: PLAGL1 (ZAC, LOT1), LATS1 (WARTS), IGF2R (MPRI) On chromosome 7: ST7 (TSG7, RAY1, FAM4A1, HELG) On chromosome 8: SFRP1 (FRP), DLC1 (ARHGAP7), BNIP3L, RHOBTB2 (DBC2), LZTS1 (FEZ1, F37), MTUS1 (ATIP), MCPH1 (BRIT1), ST18 On chromosome 9: CDKN2A (p16INK4A, p14ARF), SYK, DAB2IP (AIP1), TSC1 On chromosome 10: PTEN (MMAC1), PDCD4, MGMT On chromosome 11: WT1, TSG101, CDKN1C (BWS), CST6, IGSF4 (TSLC1, ST17, NECL2), ATM, LOH11CR2A (BCSC1) On chromosome 12: CCND2 On chromosome 13: LATS2, BRCA2 (FANCD1), RB1, ARL11 (ARLTS1) On chromosome 15: RAD51 (RECA) On chromosome 16: TSC2, CYLD, CTCF, CDH1, TERF2 (TRF2), TERFIP, FBXL8, LRRC29, ATBF1, WWOX, FBXO31, CBFA2T3 transcript B (MTG16), CPNE7, CDK10 (PISSLRE), FANCA, GAS11, C16ORF3 On chromosome 17: MAP2K4 (SERK1, SEK1, PRKMK4, MEK4), GABARAP, TP53, IC1, OVCA1(DPH1, DPH2L1), BRCA1, BECN1, SLC9A3R1 On chromosome 18: EPB41L3, SMAD4 (ELAC1, DPC4), DCC On chromosome 19: STK11 (LKB1) On chromosome 22: SMARCB1 (BAF47, SNF5), TMPRSS6, RRP22, CHEK2 (CHK2, RAD53), NF2, PRR5 From marziyeh.mojbafan from gmail.com Thu Oct 2 08:49:10 2008 From: marziyeh.mojbafan from gmail.com (marziyeh mojbafan) Date: Thu Oct 2 09:19:44 2008 Subject: [Cell-biology] help me in transfection Message-ID: Dear Paraic Kenny I am a master of science university student in genetics and my thesis is about Transfection of p19 cells with a kind of plasmid which give resistancy to Hygromycine to the transfected cells. I used lipofectamine for Transfection of my cells. I have a question; How long should we use this antibiotic to our cellular passages? I highly appreciate you if you reply my e-mail Best regards *Marziyeh Mojbafan* From info from noster-it.com Fri Oct 3 01:25:03 2008 From: info from noster-it.com (info@noster-it.com) Date: Fri Oct 3 11:48:46 2008 Subject: [Cell-biology] Join the Fastest Growing Bio Community Message-ID: <3ad9c404-bc76-4a24-ae40-704a163b8a64@f63g2000hsf.googlegroups.com> Join the Fastest Growing Bio Community - you know Facebook, Xing or MySpace, LinkedIn. In biology it exists as well: Visit academic portal http://biospace.ethz.ch register, confirm registration in email - join group, institute, create own group of interesst and share data, contacts, micorscope images, build network. You can be first and can create a future scientific group of interests. ETH University Zuerich Team From jjandova from azcc.arizona.edu Thu Oct 9 11:28:02 2008 From: jjandova from azcc.arizona.edu (Jana Jandova) Date: Thu Oct 9 13:16:28 2008 Subject: [Cell-biology] Murine mtDNA Message-ID: Hi Michael, I was wondering whether you found the program that will find the restriction sites of mouse mtDNA. I was able to find only the sequence but not the map. If you can help me it would be great. Thank you very much!!! Jana ________________________________ DISCLAIMER: This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. From nick.theodorakis from gmail.com Fri Oct 10 10:24:36 2008 From: nick.theodorakis from gmail.com (Nick Theodorakis) Date: Fri Oct 10 15:20:13 2008 Subject: [Cell-biology] George Palade dies Message-ID: <4169977a-8eb6-4427-84b6-8b49323545ae@o40g2000prn.googlegroups.com> NY Times article: LOS ANGELES =97 George E. Palade, whose discoveries about the intricate inner workings of cells helped give birth to the field of modern cell biology and earned him a Nobel Prize, died Tuesday at his home in Del Mar, Calif., at 95 . . . . ----- Nick -- Nick Theodorakis nick_theodorakis@hotmail.com contact form: http://theodorakis.net/contact.html From aumesh from stanford.edu Tue Oct 14 19:27:51 2008 From: aumesh from stanford.edu (Anita Umesh) Date: Tue Oct 14 20:16:08 2008 Subject: [Cell-biology] Dissolving lysophosphatidic acid Message-ID: Hi, I need some help dissolving lysophosphatidic acid so that it can be applied to cells. It seems to vary each time it is dissolved (final solution contains calcium (2mM) and magnesium (1mM). I am first dissolving it in a solution that contains fatty acid free bsa (1mM LPA in 1mM BSA containing PBS). I am sonicating this solution, as well as the calcium and magnesium containing final buffer. but it seems that the response (Calcium imaging using fura-2) that I am getting from the cells is variable - also seem to have to use pretty high concentrations >10uM in order to get a robust response. I notice that at higher concentrations of LPA, that my chamber containing the cells and the solution has precipitates in it by the time I am done imaging. Since people have used this to get calcium transients from cells, there must be a trick that I do not know about. Please help! Thank you,Anita --