From owner-7tms_r@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!CELLBIO.DUKE.EDU!stephen_ferguson From: stephen_ferguson@CELLBIO.DUKE.EDU ("Stephen Ferguson") Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 6 Sep 1995 09:52:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Stephen Ferguson Fergu007@mc.duke.edu please add my name ab=nd address to your list of subscribers From owner-7tms_r@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!ALCOR.BMS.COM!krystek From: krystek@ALCOR.BMS.COM (Stanley Krystek) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCR Conference Date: 6 Sep 1995 11:15:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509061812.AA11991@alcor> NNTP-Posting-Host: net.bio.net I would like to let people know about the IBC conference conference on GPCR's scheduled for December 11-13th in Philadelphia, PA. The conference is divided into the following sections: I Agonists & Antagonists Chairperson: Elizabeth Sugg (Glaxo Welcome) II Mapping Small Molecule Binding Sites Chairperson: Catherine Strader (Merck Research Laboratories) III Molecular Pharmacology of Peptide Receptors Chairperson: David Saussy (Glaxo Welcome) IV Inverse Agonism Chairperson: Michael Dennis (BioSignal, Inc.) V Understanding How G-Protein Receptors Tlak to Effectors Chairperson: David Manning (University of Pennsylvania) VI New Pathways of Signal Transduction Chairperson: Rita Huff (The Upjohn Company) The Final day of the conference is devoted to a workshop entitled: VII Structure, Modeling and Biophysics of GPCRs Chairperson: Eric Parker (Bristol-Myers Squibb) Chairperson: Stanley Krystek (Bristol-Myers Squibb) For Registration information you can contact IBC (see below) and for specific information on each section the Chairperson is listed. For more information or to receive a complete program brochure on the conference, please call IBC, Customer Service at (508)481-6400 Fax Number: (508) 481-4473 stan Stanley R. Krystek, Jr., Ph.D. Bristol-Myers Squibb Pharmaceutical Research Institute Department of Macromolecular Modeling P.O. Box 4000, Room H.3812, Princeton, N.J. 08543-4000 (609) 252-4100 FAX: (609) 252-6030 INTERNET: krystek@bms.com From owner-7tms_r@net.bio.net Thu Sep 07 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!howland.reston.ans.net!Germany.EU.net!zib-berlin.de!news.rrz.uni-hamburg.de!fokus.uke.uni-hamburg.de!METHNER From: methner@uke.uni-hamburg.de Newsgroups: bionet.molbio.proteins.7tms_r Subject: Ligands for a 50% homolog to the fmlp receptors Date: 8 Sep 1995 15:56:05 GMT Organization: University Hospital Eppendorf, Hamburg, Germany Lines: 25 Message-ID: <42pp2l$p38@rzsun02.rrz.uni-hamburg.de> Reply-To: methner@uke.uni-hamburg.de NNTP-Posting-Host: fokus.uke.uni-hamburg.de Hi everybody out there, I recently cloned a GPCR with a 50% homology to the fmlp receptor from a neuroblastoma cell line by degenerate PCR. I'd be grateful for any suggestions concerning ligands for this receptor. Do you think I could use the Xenopus oocyte system for ligand testing. Thank you Axel ================================================================ Wieso, Weshalb, Warum Wer nicht fragt bleibt dumm ================================================================ Axel Methner, M.D. Center for Molecular Neurobiology UKE-Haus 42 Martinistr. 52 D-22 246 Hamburg / Germany Tel: +49 40 47 17 50 75 Fax: +49 40 47 17 51 01 E-mail: Methner@uke.uni-hamburg.de From owner-7tms_r@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!news.sprintlink.net!in1.uu.net!EU.net!sun4nl!news.euro.net!usenet From: "Andre W. Schram" Newsgroups: bionet.molbio.proteins.7tms_r Subject: ANNOUNCEMENT: PROTEIN BIOTECHNOLOGY COURSE Date: 10 Sep 1995 16:43:52 GMT Organization: BioUpdate Foundation Lines: 26 Message-ID: <42v4k8$1l5@news.euro.net> NNTP-Posting-Host: dial6.nedernet.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news://news.nedernet.nl/bionet.molbio.proteins.7tms_r Protein Biotechnology Course held in the Netherlands October 30 - November 3 1995 Organised by the BioUpdate Foundation, the Netherlands Studies of the biochemistry of disease, coupled with advances in recombinant DNA technology have led to massive commercial interest in the production of highly purified, stable proteins. Apart from protein therapeutics, expanding markets also exist in diagnostics, industrial enzymes as biocatalysts, veterinary products and molecular biology reagents. This protein biotechnology course addresses developments in protein technology from protein extraction, through the various protein purification stages, to their presentation as stable products. The respective merits of various methods for protein characterisation and quality control are compared, issues of protein stability are treated in detail, and topics of current interest, such as protein engineering and protein glycosylation are reviewed. More information available on the BioUpdate homepage: http://www.nedernet.nl/bioupdate/index.html From owner-7tms_r@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!MAIL.MED.UPENN.EDU!brass From: brass@MAIL.MED.UPENN.EDU (Lawrence F. Brass) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: How to Get Free Cable TV..LEGIT Date: 11 Sep 1995 05:59:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >First I want to say I am sorry that this has nothing >to do with the topic of this group. You may see this >message in other newsgroups, the reason is I am subscribed >to those too so please dont flame me. I have been posting >and reading to this group for a while. I just wanted to >let you people out know there is a way to get Cable TV for >FREE. I recieved this letter in the mail from a friend >that had told me it works. I gave this to one of my friends >and he had free cable in his house within 20 mins. I have not >tried this method yet but may soon try if my cable goes up in >price. If anyone would like a copy send the following to the >address below. > flame you, indeed. This stuff has no business being here, irregardless of whether it is legal, a scam or simply misleading. ******************************************** * Lawrence Brass, MD PhD * * University of Pennsylvania * * Dept of Medicine * * CRB 678, 415 Curie Blvd * * Phila., PA 19104 * * brass@mail.med.upenn.edu * * 215-573-3540 phone * * 215-573-2189 fax * ******************************************** From owner-7tms_r@net.bio.net Mon Sep 11 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsserver.jvnc.net!synapse.bms.com!seiler.bms.com!user From: Seiler_S@msmail.bms.com (Steve Seiler) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Tightly-bound agonists Followup-To: bionet.molbio.proteins.7tms_r Date: Tue, 12 Sep 1995 13:12:15 -0400 Organization: BMS Lines: 19 Message-ID: NNTP-Posting-Host: seiler.bms.com Esteemed Netters, Are there any explanations as to why some agonists bind to their G-coupled receptors extremely tightly (usually studied under the conditions of low temp, presence of detergents, etc.) while antagonist binding seems to be relatively unaffected? Any explanation for the existence of the tight-binding states? Is there physiological significance to these tight-binding states? Any good discussions of the subject in the literature? Thanks for your thoughts. /Steve -- Steven Seiler Seiler_Steven_M.PRILVMS3@msmail.bms.com Dept of Pharmacology Bristol Myers Squibb Co Princeton, NJ From owner-7tms_r@net.bio.net Mon Sep 11 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!news-feed-1.peachnet.edu!news.cc.emory.edu!usenet From: Newsgroups: bionet.molbio.proteins.7tms_r Subject: Two-hybrids for 7tms_r ?? Date: 13 Sep 1995 02:04:43 GMT Organization: Emory University Lines: 8 Message-ID: <435e7r$h2r@moe.cc.emory.edu> NNTP-Posting-Host: jbear-sl.cc.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.molbio.proteins.7tms_r Has anyone heard if using the intracellular domains (loops or tails) of multi-pass transmembrane proteins (specifically g-prot. coupled receptors) for two-hybrids has worked? Has anyone tried it? I can't find any examples of this in the literature. Help. Jim Bear- Saxe lab, Emory University From owner-7tms_r@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!OCELOT.RUTGERS.EDU!WCUI From: WCUI@OCELOT.RUTGERS.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscription to discussion group mailing Date: 13 Sep 1995 12:57:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HV8E8J0OAC9ZM1WI@mbcl.rutgers.edu> NNTP-Posting-Host: net.bio.net I would like to be a member of this discussion group. I am a computational chemist working at Synaptic pharmaceutical Corp. My main interest is the structure of the GPCR receptors. Thanx. From owner-7tms_r@net.bio.net Wed Sep 13 23:00:00 1995 Path: biosci!MED.UNC.EDU!erweiss From: erweiss@MED.UNC.EDU ("Ellen R. Weiss") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral postion available in my lab Date: 14 Sep 1995 16:14:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509142312.AA06789@med.unc.edu> Reply-To: "Ellen R. Weiss" NNTP-Posting-Host: net.bio.net I have a postdoctoral postion available to investigate the regulation of signal transduction pathways activated by rhodopsin at the molecular level. Studies will focus on mutagenesis, in vitro expression and protein-protein interaction to identify sites critical for G protein coupling and desensitization through phosphorylation. Please send curriculum vitae and three letters of recommendation to: Dr. Ellen R. Weiss, Department of Cell Biology and Anatomy, The University of North Carolina at Chapel Hill, CB# 7090, 108 Taylor Hall, Chapel Hill, NC 27599. An Equal Opportunity/Affirmative Action Employer. Recent publications: Weiss, E. R., Osawa, S., Shi, W. and Dickerson, C. D. (1994) Effects of carboxyl-terminal truncation on the stability and G protein coupling activity of bovine rhodopsin. Biochemistry 33, 7587-7593. Osawa, S. and Weiss, E. R. (1994) The carboxyl terminus of bovine rhodopsin is not required for G protein activation. Mol. Pharmacol. 46, 1036-1040. Dickerson, C. D. and Weiss, E. R. (1995) The coupling of pertussis toxin-sensitive G proteins to phospholipase A2 and adenylyl cyclase in CHO cells expressing bovine rhodopsin. Exp. Cell Res. 216, 46-50. Shi, W., Osawa, S., Dickerson, C. D. and Weiss, E. R. (1995) Rhodopsin mutants distinguish sites important for the activation of rhodopsin kinase and Gt. J. Biol. Chem. 270, 2112-2119. From owner-7tms_r@net.bio.net Mon Sep 18 23:00:00 1995 Path: biosci!tcrl.jti.co.jp!hirano From: hirano@tcrl.jti.co.jp ("Daisuke Hirano") Newsgroups: bionet.molbio.proteins.7tms_r Subject: info Date: 19 Sep 1995 16:37:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509192336.AA28061@ns.tcrl.jti.co.jp> NNTP-Posting-Host: net.bio.net info info 7tms_r@net.bio.net From owner-7tms_r@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.swt.edu!pirates.cs.swt.edu!swrinde!emory!usenet From: medtjm@bimcore.emory.edu (T. J. Murphy) Newsgroups: bionet.molbio.proteins.7tms_r Subject: FYI: AHA Web site Date: 25 Sep 1995 21:21:05 GMT Organization: Biomolecular Computing Resource, Emory University Lines: 17 Distribution: world Message-ID: <4476g1$ns1@emory.mathcs.emory.edu> Reply-To: medtjm@bimcore.emory.edu NNTP-Posting-Host: bimcore.emory.edu See the American Heart Association's pages, it looks very comprehensive for scientists and lay alike: http://www.amhrt.org/ --- TJ Murphy Asst. Professor Dept of Pharmacology Emory University School of Medicine From owner-7tms_r@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!VENUS.HILI.COM!forray From: forray@VENUS.HILI.COM Newsgroups: bionet.molbio.proteins.7tms_r Subject: Suscribe to 7tms_r Date: 26 Sep 1995 19:13:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509270445.AA0022@ppp-nj-31.hili.com> Reply-To: forray@venus.hili.com NNTP-Posting-Host: net.bio.net I would appreciate if you could add my e-mail address to the 7tms_r group. Thanks Carlos //---------------------------------------------------------------------------- // Carlos Forray Claps // Paramus, NJ // e-mail forray@venus.hili.com From owner-7tms_r@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: microplate@aol.com (MICROPLATE) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Luminometers Date: 27 Sep 1995 19:31:29 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: root@newsbf02.news.aol.com Message-ID: <44cmsh$ids@newsbf02.news.aol.com> References: <950927111715_75763.2141_BHQ43-1@CompuServe.COM> Reply-To: microplate@aol.com (MICROPLATE) NNTP-Posting-Host: newsbf02.mail.aol.com Suggest you look at Dynatech in Arlington Virginia, they have a 800 number check it out on 1800 555 1212. Also Packard Instrument in Meriden CT. Good luck Roy L. Manns Polyfiltronics home office. Time is precious, enjoy it helping others. From owner-7tms_r@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!UMICH.EDU!mccauley From: mccauley@UMICH.EDU ("Laurie K. McCauley") Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-doc position Date: 27 Sep 1995 06:28:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITION UNIVERSITY OF MICHIGAN Departments of Hematology and Oncology and Periodontics/Prevention/Geriatrics A POSTDOCTORAL POSITION is available immediately to study mechanisms of PTH/PTHrP receptor regulation in bone metastasis associated with prostate cancer. The candidate will join a multidisciplinary group of investigators in the Comprehensive Cancer Center in a Specialized Program of Research Excellence in Prostate Cancer. Experience in cellular and molecular biology required. Send curriculum vitae and names of three references to: Laurie McCauley, University of Michigan, 1011 N. University Ave. Ann Arbor, MI 48109-1078, Telephone (313) 747-3206, FAX (313) 763-5503, e-mail: mccauley@umich.edu From owner-7tms_r@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!COMPUSERVE.COM!75763.2141 From: 75763.2141@COMPUSERVE.COM ("Jeremy I. Paul") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Luminometers Date: 27 Sep 1995 04:21:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <950927111715_75763.2141_BHQ43-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net I'm looking for some input from forum participants on luminometers that you have experience with out there. I'm especially interested in knowing about microplate type readers - their relative benefits and their various drawbacks. Thanks- Jeremy Paul From owner-7tms_r@net.bio.net Wed Sep 27 23:00:00 1995 Path: biosci!PPP.BEKKOAME.OR.JP!sannai From: sannai@PPP.BEKKOAME.OR.JP (Hiroshi TAKEDA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 28 Sep 1995 13:07:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509282004.FAA04633@bekkoame.bekkoame.or.jp> NNTP-Posting-Host: net.bio.net help From owner-7tms_r@net.bio.net Thu Sep 28 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!tank.news.pipex.net!pipex!warwick!lyra.csx.cam.ac.uk!cna20 From: cna20@cus.cam.ac.uk (Chris Airriess) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Antisense oligos to hamster Gs-alpha Date: 29 Sep 1995 15:40:38 GMT Organization: University of Cambridge, England Lines: 12 Message-ID: <44h41m$9a2@lyra.csx.cam.ac.uk> NNTP-Posting-Host: bootes.cus.cam.ac.uk Does anyone know of a commercial source for antisense oligos to Chinese hamster Gs-alpha? Rat, mouse and human are available from Biognostik but I would prefer to use the hamster. Thanks. Chris Airriess -- ----------------------------------------------------------------------- Dr. C.N. Airriess Tel +44 (0) 223 336604 Laboratory of Molecular Signalling Fax +44 (0) 223 461954 Department of Zoology From owner-7tms_r@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!soap.news.pipex.net!pipex!usenet From: M J Geisow Newsgroups: bionet.molbio.proteins.7tms_r Subject: G-proteins in EU Framework III and Conference Date: 1 Oct 1995 10:45:41 GMT Organization: UnipalmPIPEX server (post doesn't reflect views of UnipalmPIPEX) Lines: 26 Message-ID: <44lrgl$b4a@soap.news> NNTP-Posting-Host: am079.du.pipex.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1 (Windows; I; 16bit) If you are thinking of applying for a grant in the EU Fourth Framework (Structural Biology Area) You might like to read the World Wide Web document: http://www.cryst.bbk.ac.uk/CEC/cec.html This was prepared as a concise summary for UK applicants and was checked by the European Commission. Please also note that the scientific programme for the major international Protein Engineering meeting in Montpellier, France 2-6 March 1996 is now finalised. A CEC GRANT has been applied for to support attendance by all 151 Framework 3 Structural Biology grant holders. Information and registration at: http://www.cryst.bbk.ac.uk/CEC/pope5.html Finally, note that there is now a CALENDAR of World Structural Biology Events at: http://www.cryst.bbk.ac.uk/CEC/calendar.html NOTE: PLEASE ENTER THE ADDRESSES PRESERVING THE LETTER CASE AS SHOWN. M J Geisow Sept 1995 UK LINK Protein Engineering Programme Co-ordinator