From owner-7tms_r@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!swrinde!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!news.cc.ic.ac.uk!cplm1.bc.ic.ac.uk!user From: m.witty@ic.ac.uk (Mike Witty) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gustatory receptor Date: Wed, 04 Oct 1995 08:54:31 +0000 Organization: Imperial College, London, UK Lines: 5 Message-ID: NNTP-Posting-Host: cplm1.bc.ic.ac.uk Dear All, is there anyone out there interested in collaboration regarding isolation of clones for human taste receptors. I have a probe, but need a human toungue expression library. Using human tissues in my non-medical department causes all kinds of regulatory problems. Please reply to: mbwit@seqnet.dl.ac.uk From owner-7tms_r@net.bio.net Wed Oct 04 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: UBCG91C@CCS.BBK.AC.UK Newsgroups: bionet.molbio.proteins.7tms_r Subject: meeting announcement Date: 5 Oct 1995 10:50:06 +0100 Lines: 66 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4509oe$sd6@mserv1.dl.ac.uk> Original-To: 7TMS_R@dl.AC.UK BIOLOGICAL STRUCTURES GROUP BCA WINTER MEETING 13th DECEMBER 1995 10.30 am at BIRKBECK COLLEGE University of London ***************************************************************** * * * M E M B R A N E A N D S U R F A C E P R O T E I N S * * * ***************************************************************** Topics will include: Membrane Protein Expression Systems for Structural Studies Electron Diffraction of 2D Membrane Protein Crystals Modelling of Transmembrane Proteins & Receptors New Membrane Protein Structures by Xray, EM, and NMR Plenary lecturer: H. MICHEL, Max Planck Institute Organiser: Dr. B. A. WALLACE BIRKBECK COLLEGE University of London London, U.K. Please submit the enclosed application and a cheque BY POST. ------------------------------------------------------------------------------ APPLICATION FORM: (BLOCK CAPITALS PLEASE) Title: Prof/Dr/Mr/Mrs/Ms/(other)......... Name:............................... Address for Correspondence:................................................... ............................................................................... ............................................................................... Phone No:............ Fax No:............ E-mail:............................. I enclose a cheque* made payable to: BSG-BCA for the amount of: Pounds Please Tick BCA Member . . . . . . . . . . . . . . . . . . . . 35 _______ Non-Member . . . . . . . . . . . . . . . . . . . . 47 _______ BCA Student Member (See Below) . . . . . . . . . . 16 _______ Student Non-Member (See Below) . . . . . . . . . . 22 _______ (* This is inclusive of Lunch and Tea) FOR STUDENTS: I confirm the above named is eligible for student status. Signed:....................................Supervisor/Tutor/Head of Department Please return this application BEFORE 1ST DECEMBER to: Dr. B. A. WALLACE, Dept. of Crystallography, Birkbeck College, University of London, Malet Street, London, WC1E 7HX, UK. From owner-7tms_r@net.bio.net Wed Oct 04 23:00:00 1995 Path: biosci!ITSA.UCSF.EDU!barber From: barber@ITSA.UCSF.EDU (Diane Barber) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 5 Oct 1995 12:01:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am interested in subscribing to the newsgroup. Could someone please send me information? Diane Barber barber@itsa.ucsf.edu From owner-7tms_r@net.bio.net Sat Oct 07 23:00:00 1995 Path: biosci!novo.dk!byw From: byw@novo.dk (Robert Bywater) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 8 Oct 1995 02:59:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510080956.LAA09758@bach> NNTP-Posting-Host: net.bio.net The message from md@goodnet.com (Mike Denton) should never have been sent to this group. It is very likely a fraud, but in any case, it has nothing to do with '7TMs'. We need better quality-control on what is sent over the net to this group. Robert Bywater GPCR-researcher at Novo Nordisk A/S, Denmark. ****************************** * * * Robert Bywater * * * * Biostructure Department * * NOVO NORDISK A/S * * * * DK-2880 BAGSVAERD Denmark * * * * email byw@novo.dk * * fax :: +45 4442 1400 * ****************************** From owner-7tms_r@net.bio.net Sat Oct 07 23:00:00 1995 Path: biosci!RECEPTOR.MGH.HARVARD.EDU!lfk From: lfk@RECEPTOR.MGH.HARVARD.EDU (Lee F. (Frank) Kolakowski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation of the Group is Possible! Date: 8 Oct 1995 08:55:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510081544.AA03488@receptor> References: <199510080956.LAA09758@bach> NNTP-Posting-Host: net.bio.net Dear 7tms_r Netters, Messages like the one posted today are very common throughout the Internet. On 8 Oct 1995 02:59:00 -0700, byw@novo.dk (Robert Bywater) said: > The message from md@goodnet.com (Mike Denton) should never have > been sent to this group. It is very likely a fraud, but in any > case, it has nothing to do with '7TMs'. > We need better quality-control on what is sent over the net to this > group. The only solution to this problem is to change the group from one which is *un*moderated (all posts go to the mailing list and the newsgroup) to one which is moderated (all posts go to moderator, who then *reposts* messages which are appropriate for the group. This moderated route should be fine, but is not a job that I would be able to perform at this time. Let's discuss this possibility. One or two events a month are not too bad (IMHO) but might be more bothersome to those on the mail-list. Thank you for your opinions. Frank Kolakowski Email: lfk@receptor.mgh.harvard.edu 617-355-7515 (LAB) GCRDb-WWW As of November 1, 1995: Lee F. (Frank) Kolakowski, Jr., Ph.D. Dept of Pharmacology Univ Texas Health Science Center 7703 Floyd Curl Drive San Antonio, TX 78284-7764 ph: 210-567-4233 FAX: 210-567-4303 E-mail: kolakowski@uthscsa.edu lfk@receptor.mgh.harvard.edu See also: http://receptor.mgh.harvard.edu/GCRDBHOME.html From owner-7tms_r@net.bio.net Sun Oct 08 23:00:00 1995 Path: biosci!COMPUSERVE.COM!75763.2141 From: 75763.2141@COMPUSERVE.COM ("Jeremy I. Paul") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderated Forums Date: 9 Oct 1995 07:01:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <951009135528_75763.2141_BHQ75-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net Dear 7tms_r forum members- I am not in favor of moderating this forum since it smacks of a kind of oversight and "big brotherism" that we simply do not need. I susbscribe to this foum because I want to read everything in it in an unexpurgated form. I would much rather deal with the occasional piece of junk mail myself than cede that responsibility to anyone else. -Jeremy Paul From owner-7tms_r@net.bio.net Sun Oct 08 23:00:00 1995 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation in all things? Date: 9 Oct 1995 11:32:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: On 10/8/95, Lee ( Frank ) Kolalowski wrote: ------------------------------------------- >The only solution to this problem is to change the group from one >which is *un*moderated (all posts go to the mailing list and the >newsgroup) to one which is moderated (all posts go to moderator, who >then *reposts* messages which are appropriate for the group. > >This moderated route should be fine, but is not a job that I would be >able to perform at this time. > >Let's discuss this possibility. One or two events a month are not too >bad (IMHO) but might be more bothersome to those on the mail-list. Consider me as one vote AGAINST moderating this newsgroup. I subscribe to the listserver and read it through a newsserver. To date the volume of "offensive" mail has been minimal. IMHO, the 7tms_r group is functioning fine as is. John ------------------------------------------------------------------------ A. John Watson, Ph.D. __ __ __ Bristol-Myers Squibb Company /__/__/__/\ Pharmaceutical Reseach Institute /__/__/__/\/\ Central Nervous System Biology, Department 404 /__/__/__/\/\/\ 5 Research Parkway \__\__\__\/\/\/ Wallingford, CT 06492 \__\__\__\/\/ \__\__\__\/ watson_j@bms.com | 203.284.6745 | Standard Disclaimers Apply 203.284.7569 fax | ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.uiowa.edu!uunet!in2.uu.net!noc.near.net!das-news2.harvard.edu!oitnews.harvard.edu!bloch.nmr.mgh.harvard.edu!nntp.mgh.harvard.edu!lfk From: lfk@receptor.mgh.harvard.edu (Lee F. (Frank) Kolakowski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Excerpted TOC from JBC (October) Date: 11 Oct 1995 13:02:18 GMT Organization: Mass. General Hospital, Renal Unit Lines: 211 Distribution: world Message-ID: NNTP-Posting-Host: receptor.mgh.harvard.edu AU Daly-T-J. LaRosa-G-J. Dolich-S. Maione-T-E. Cooper-S. Broxmeyer-H-E. TI High activity suppression of myeloid progenitor proliferation by chimeric mutants of interleukin 8 and platelet factor 4 SO J-Biol-Chem. 1995 Oct 6. 270(40). p 23282. AU Chen-J. Bernstein-H-S. Chen-M. Wang-L. Ishii-M. Turck-C-W. Coughlin-S-R. TI Tethered ligand library for discovery of peptide agonists SO J-Biol-Chem. 1995 Oct 6. 270(40). p 23398. AU Torphy-T-J. Zhou-H-L. Foley-J-J. Sarau-H-M. Manning-C-D. Barnette-M-S. TI Salbutamol up-regulates PDE4 activity and induces a heterologous desensitization of U937 cells to prostaglandin E2. Implications for the therapeutic use of {beta}-adrenoceptor agonists. SO J-Biol-Chem. 1995 Oct 6. 270(40). p 23598. AU Zucker-S. Conner-C. DiMassmo-B-I. Ende-H. Drews-M. Seiki-M. Bahou-W-F. TI Thrombin induces the activation of progelatinase A in vascular endothelial cells. Physiologic regulation of angiogenesis. SO J-Biol-Chem. 1995 Oct 6. 270(40). p 23730. AU Ahmed-M-U. Hazeki-K. Hazeki-O. Katada-T. Ui-M. TI Cyclic AMP-increasing agents interfere with chemoattractant-induced respiratory burst in neutrophils as a result of the inhibition of phosphatidylinositol 3-kinase rather than receptor-operated Ca2+ influx SO J-Biol-Chem. 1995 Oct 6. 270(40). p 23816. AU Xu-Y. Ware-J-A. TI Selective inhibition of thrombin receptor-mediated Ca2+ entry by protein kinase C {beta} SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 23887. AU Yu-K. Bayona-W. Kallen-C-B. Harding-H-P. Ravera-C-P. McMahon-G. Brown-M. Lazar-M-A. TI Differential activation of peroxisome proliferator-activated receptors by eicosanoids SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 23975. AU Wayman-G-A. Hinds-T-R. Storm-D-R. TI Hormone stimulation of type III adenylyl cyclase induces Ca2+ oscillations in HEK-293 cells SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24108. AU Seufferlein-T. Rozengurt-E. TI Sphingosylphosphorylcholine activation of mitogen-activated protein kinase in Swiss 3T3 cells requires protein kinase C and a pertussis toxin-sensitive G protein SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24334. AU Seufferlein-T. Rozengurt-E. TI Sphingosylphosphorylcholine rapidly induces tyrosine phosphorylation of p125FAK and paxillin, rearrangement of the actin cytoskeleton and focal contact assembly. Requirement of p21rho in the signaling pathway SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24343. AU Colombo-M-I. Inglese-J. D'Souza-Schorey-C. Beron-W. Stahl-P-D. TI Heterotrimeric G proteins interact with the small GTPase ARF. Possibilities for the regulation of vesicular traffic SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24564. AU Ptasznik-A. Traynor-Kaplan-A. Bokoch-G-M. TI G protein-coupled chemoattractant receptors regulate Lyn tyrosine kinase Shc adapter protein signaling complexes. Vol. 270 (1995) 19969-19973 SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24622. AU Mitchell-L-W. Volin-M. Jaffe-E-K. TI The phylogenetically conserved histidines of Escherichia coli porphobilinogen synthase are not required for catalysis SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24054. AU Calvert-P-D. Klenchin-V-A. Bownds-M-D. TI Rhodopsin kinase inhibition by recoverin. Function of recoverin myristoylation SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24127. AU Azarian-S-M. King-A-J. Hallett-M-A. Williams-D-S. TI Selective proteolysis of arrestin by calpain. Molecular characteristics and its effect on rhodopsin dephosphorylation SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24375. AU Puri-R-N. Kumar-A. Chen-H. Colman-R-F. Colman-R-W. TI Inhibition of ADP-induced platelet responses by covalent modification of aggregin, a putative ADP receptor, by 8-(4-bromo-2,3-dioxobutylthio)ADP SO J-Biol-Chem. 1995 October 13, 1995. 270(41). p 24482. AU Buhl-A-M. Johnson-N-L. Dhanasekaran-N. Johnson-G-L. TI G{alpha}12 and G{alpha}13 stimulate Rho-dependent stress fiber formation and focal adhesion assembly SO J-Biol-Chem. 1995 October 20. 270(42). p 24631. AU Ferguson-S-S-G. Menard-L. Barak-L-S. Koch-W-J. Colapietro-A-M. Caron-M-G. TI Role of phosphorylation in agonist-promoted {beta}2-adrenergic receptor sequestration. Rescue of a sequestration-defective mutant receptor by {beta}ARK1 SO J-Biol-Chem. 1995 October 20. 270(42). p 24782. AU Eto-A. Akita-Y. Saido-T-C. Suzuki-K. Kawashima-S. TI The role of the calpain-calpastatin system in thyrotropin-releasing hormone-induced selective down-regulation of a protein kinase C isozyme, nPKC{epsilon}, in rat pituitary GH4C1 cells SO J-Biol-Chem. 1995 October 20. 270(42). p 25115. AU Rodriguez-C-G. Cundell-D-R. Tuomanen-E-I. Kolakowski-L-F-Jr.. Gerard-C. Gerard-N-P. TI The role of N-glycosylation for functional expression of the human platelet-activating factor receptor. Glycosylation is required for efficient membrane trafficking SO J-Biol-Chem. 1995 October 20. 270(42). p 25178. AU Dizhoor-A-M. Olshevskaya-E-V. Henzel-W-J. Wong-S-C. Stults-J-T. Ankoudinova-I. Hurley-J-B. TI Cloning, sequencing, and expression of a 24-kDa Ca2+-binding protein activating photoreceptor guanylyl cyclase SO J-Biol-Chem. 1995 October 20. 270(42). p 25200. AU Manser-E. Chong-C. Zhao-Z-S. Leung-T. Michael-G. Hall-C. Lim-L. TI Molecular cloning of a new member of the p21-Cdc42/Rac-activated kinase (PAK) family SO J-Biol-Chem. 1995 October 20. 270(42). p 25070. AU Hershey-J-C. Hautmann-M. Thompson-M-M. Rothblum-L-I. Haystead-T-A-J. Owens-G-K. TI Angiotensin II-induced hypertrophy of rat vascular smooth muscle is associated with increased 18 S rRNA synthesis and phosphorylation of the rRNA transcriptional factor, upstream binding factor SO J-Biol-Chem. 1995 October 20. 270(42). p 25096. AU Perlman-J-H. Wang-W. Nussenzveig-D-R. Gershengorn-M-C. TI A disulfide bond between conserved extracellular cysteines in the thyrotropin-releasing hormone receptor is critical for binding SO J-Biol-Chem. 1995 October 20. 270(42). p 24682. AU Eason-M-G. Liggett-S-B. TI Identification of a Gs coupling domain in the amino terminus of the third intracellular loop of the {alpha}2A-adrenergic receptor. Evidence for distinct structural determinants that confer Ga versus Gi coupling SO J-Biol-Chem. 1995 October 20. 270(42). p 24753. AU HYVONEN-M. MACIAS-M-J. NILGES-M. OSCHKINAT-H. SARASTE-M. WILMANNS-M. TI Structure of the binding site for inositol phosphates in a PH domain. SO EMBO-J. 1995 OCTOBER 2. 14(19). P.4676-4685. AU KALKBRENNER-F. DEGTIAR-V-E. SCHENKER-M. BRENDEL-S. ZOBEL-A. HESCHLER-J. WITTIG-B. SCHULTZ-G. TI Subunit composition of Go proteins functionally coupling galanin receptors to voltage-gated calcium channels. SO EMBO-J. 1995 OCTOBER 2. 14(19). P.4728-4737. AU Karoor-V. Baltensperger-K. Paul-H. Czech-M-P. Malbon-C-C. TI Phosphorylation of tyrosyl residues 350/354 of the {beta}-adrenergic receptor is obligatory for counterregulatory effects of insulin SO J-Biol-Chem. 1995 October 27. 270(43). p 25305. AU Szabo-M-C. Soo-K-S. Zlotnik-A. Schall-T-J. TI Chemokine class differences in binding to the Duffy antigen-erythrocyte chemokine receptor SO J-Biol-Chem. 1995 October 27. 270(43). p 25348. AU Kisselev-O. Ermolaeva-M. Gautam-N. TI Efficient interaction with a receptor requires a specific type of prenyl group on the G protein {gamma} subunit SO J-Biol-Chem. 1995 October 27. 270(43). p 25356. AU Berven-L-A. Crouch-M-F. Katsis-F. Kemp-B-E. Harland-L-M. Barritt-G-J. TI Evidence that the pertussis toxin-sensitive trimeric GTP-binding protein Gi2 is required for agonist- and store-activated Ca2+ inflow in hepatocytes SO J-Biol-Chem. 1995 October 27. 270(43). p 25893. AU Tjoelker-L-W. Eberhardt-C. Unger-J. Trong-H-L. Zimmerman-G-A. McIntyre-T-M. Stafforini-D-M. Prescott-S-M. Gray-P-W. TI Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad SO J-Biol-Chem. 1995 October 27. 270(43). p 25481. AU Mouillac-B. Chini-B. Balestre-M-N. Elands-J. Trumpp-Kallmeyer-S. Hoflack-J. Hibert-M. Jard-S. Barberis-C. TI The binding site of neuropeptide vasopressin V1a receptor. Evidence for a major localization within transmembrane regions. SO J-Biol-Chem. 1995 October 27. 270(43). p 25771. -- Frank Kolakowski Email: lfk@receptor.mgh.harvard.edu 617-355-7515 (LAB) GCRDb-WWW As of November 1, 1995: Lee F. (Frank) Kolakowski, Jr., Ph.D. Dept of Pharmacology Univ Texas Health Science Center 7703 Floyd Curl Drive San Antonio, TX 78284-7764 ph: 210-567-4233 FAX: 210-567-4303 E-mail: kolakowski@uthscsa.edu lfk@receptor.mgh.harvard.edu See also: http://receptor.mgh.harvard.edu/GCRDBHOME.html From owner-7tms_r@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!EA.OAC.UCI.EDU!eaug022 From: eaug022@EA.OAC.UCI.EDU (Ed Gerstin) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Help Date: 11 Oct 1995 17:34:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Sorry to have to post this, but could someone send me the information on how to change my e-mail address for this listserv? I knew how to do this at one point, but I can't seem to remember now....Thanks in advance. -Ed ------------------------------------------------------------- Ed Gerstin : To live long, eaug022@ea.oac.uci.edu : it is necessary Department of Pharmacology : to live slowly. College of Medicine : University of California, Irvine : - Cicero From owner-7tms_r@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!news.maz.net!news.omnilink.net!news.gni.net!thorin!rz.uni-karlsruhe.de!news.uni-stuttgart.de!uni-regensburg.de!fauern!rznews.rrze.uni-erlangen.de!faui0n.informatik.uni-erlangen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!krasel From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation of the Group is Possible! Date: 10 Oct 1995 18:48:45 GMT Organization: Dept. of Pharmacology, U Wuerzburg Lines: 22 Distribution: world Message-ID: <45ef6d$nii@winx03.informatik.uni-wuerzburg.de> References: <199510080956.LAA09758@bach> <9510081544.AA03488@receptor> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [version 1.2 PL2] Lee F. (Frank) Kolakowski (lfk@RECEPTOR.MGH.HARVARD.EDU) wrote: > The only solution to this problem is to change the group from one > which is *un*moderated (all posts go to the mailing list and the > newsgroup) to one which is moderated (all posts go to moderator, who > then *reposts* messages which are appropriate for the group. It is easy to bypass the moderation procedure. Recently the first newsreaders have appeared which allow everybody to post in a moderated forum without any approval. If one doesn't have this kind of software, you can still easily forge an approved message by hand. So, while moderation would protect us from the occasional spammer, it would certainly not prevent spamming by somebody _inclined_ to do so. Therefore, I am against moderation. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!UMICH.EDU!rneubig From: rneubig@UMICH.EDU (Rick Neubig) Newsgroups: bionet.molbio.proteins.7tms_r Subject: E-mail subscription information for 7tms_r Date: 12 Oct 1995 03:52:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 163 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To 7tms_r's, Frank asked me to keep an eye on this group while he moves to San Antonio (congrats on the new faculty position - Frank!). He had also asked me many months ago to help put together a FAQ (frequently asked questions) which could be posted at intervals. Anybody with suggestions for items for the FAQ, please send them to me or Frank. As a start to the FAQ, I'm reposting the subscribing information for E-mail folks. If this doesn't apply to you - please accept my apologies in advance for the use of the bandwidth. The short answer re subscribing and unsubscribing in North America is to send a message with one line to biosci-server@net.bio.net To unsubscribe it should come from your old address and say: unsubscribe 7tms_r To subscribe it should come from your new address and say: subscribe 7tms_r More detailed information follows re BIOSCI newsgroup subscriptions. Cheers, Rick _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 E-mail Subscription Requests and other Information -------------------------------------------------- For users in the Americas and Pacific Rim countries, e-mail subscription and cancellation requests are handled automatically by an e-mail server, although personal assistance is also available via the biosci-help@net.bio.net address. Once your e-mail address is added to a subscription list, mail will be sent to your address automatically each time someone posts a message. This will continue until you remove your address from the subscription list. You should first send the lists command to the address biosci-server@net.bio.net to obtain a listing of all current BIOSCI mailing lists. 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It will be necessary for you to contact biosci-help@net.bio.net and resubscribe. Please see the BIOSCI FAQ, mentioned at the beginning of this document, for more details on how BIOSCI handles addresses which reject mail. ==================== end of BIOSCI info ==================== From owner-7tms_r@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!COURRIER.USHERB.CA!mrolaple From: mrolaple@COURRIER.USHERB.CA (Marek Rola-Pleszczynski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 12 Oct 1995 10:33:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Subscribe ******************* Marek Rola-Pleszczynski,M.D. Immunologie Faculte de Medecine Universite de Sherbrooke 3001 12e Avenue N. Sherbrooke, QC J1H 5N4 Canada From owner-7tms_r@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!chempath.uct.ac.za!DAVID From: DAVID@chempath.uct.ac.za ("Dave Myburgh") Newsgroups: bionet.molbio.proteins.7tms_r Subject: XL-1 Blue E. coli.....dam+ ??? Date: 13 Oct 1995 06:26:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: Hi All Does anybody out there know whether XL-1 Blue cells are able to methylate adenine at position 6 in the sequence GATC i.e. G(m6A)TC? I have been trying to cut pcDNA/Amp and pBluescript, each with an insert containing the sequence TGATCA, which is the BclI recognition site. However, none of the plasmids gets digested. I have checked everywhere to see whether there is mention of XL-1 cells being dam+, but to no avail. We don't have any DpnI enzyme which only cuts methylated GATC sequences, so that idea won't work. Any help would be appreciated. Thanks Dave -------------------------------------------------------------------- Dave Myburgh :) :-) [|-) :( ;-) |-> Lab 227 Falmouth Bldg Dept. Chemical Pathology UCT Medical School :-)X }:) 8-) :-)> Observatory 7925 E-mail: david@chempath.uct.ac.za South Africa mybdav02@uctvms.uct.ac.za -------------------------------------------------------------------- From owner-7tms_r@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!novo.dk!jsr From: jsr@novo.dk (Jesper Rasmussen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: XL-1 Blue E. coli.....dam+ ??? Date: 14 Oct 1995 04:22:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510141120.AA07629@eagle.novo.dk> NNTP-Posting-Host: net.bio.net Hi Dave, XL-1 Blue is dam+ . The reason that its not listed in the genotype of that strain is that dam+ is the wildtype. Try transform your plasmids into a dam- strain (e.g. JM110 available from Stratagene) and prepare your DNA from there. Good luck, Jesper. >Hi All > >Does anybody out there know whether XL-1 Blue cells are able to >methylate adenine at position 6 in the sequence GATC i.e. G(m6A)TC? > >I have been trying to cut pcDNA/Amp and pBluescript, each with an >insert containing the sequence TGATCA, which is the BclI recognition >site. However, none of the plasmids gets digested. I have checked >everywhere to see whether there is mention of XL-1 cells being dam+, >but to no avail. We don't have any DpnI enzyme which only cuts >methylated GATC sequences, so that idea won't work. Any help would >be appreciated. > >Thanks > >Dave > > >-------------------------------------------------------------------- >Dave Myburgh :) :-) [|-) :( ;-) |-> >Lab 227 Falmouth Bldg >Dept. Chemical Pathology >UCT Medical School :-)X }:) 8-) :-)> >Observatory >7925 E-mail: david@chempath.uct.ac.za >South Africa mybdav02@uctvms.uct.ac.za >-------------------------------------------------------------------- ---------------------------------------------------------------------------- Jesper Rasmussen Molecular and Cellular Biology Health Care Discovery, Novo Nordisk A/S e-mail: jsr@novo.dk Phone: +45 4442 3881 Fax: +45 4444 4008 Mail: Novo Alle, 6B2.107, DK-2880 Bagsvaerd, Denmark --------------------------------------------------------------------------- From owner-7tms_r@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!swidir.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-ulm.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!uni-regensburg.de!faui0n.informatik.uni-erlangen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!krasel From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: XL-1 Blue E. coli.....dam+ ??? Date: 15 Oct 1995 19:29:59 GMT Organization: Dept. of Pharmacology, U Wuerzburg Lines: 17 Distribution: world Message-ID: <45rnfn$n8i@winx03.informatik.uni-wuerzburg.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [version 1.2 PL2] Dave Myburgh (DAVID@chempath.uct.ac.za) wrote: > Does anybody out there know whether XL-1 Blue cells are able to > methylate adenine at position 6 in the sequence GATC i.e. G(m6A)TC? Yup, I think so. It's a typical feature of WT E.coli and there are only a few strains which are not dam+. Only dam- strains are explicitely noted. Either you have to get a dam- bug (like JM110, if memory serves right) or you have to buy this damn DpnI. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!RI.NCVC.GO.JP!kame From: kame@RI.NCVC.GO.JP (Koichi Kokame) Newsgroups: bionet.molbio.proteins.7tms_r Subject: help Date: 16 Oct 1995 22:14:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510170511.AA07590@jsc.ri.ncvc.go.jp> NNTP-Posting-Host: net.bio.net help From owner-7tms_r@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!psgrain!nntp.teleport.com!usenet From: nevek@teleport.com (Kim A. Neve) Newsgroups: bionet.molbio.proteins.7tms_r,bionet.software Subject: curve-fitting software Date: 18 Oct 1995 18:23:06 GMT Organization: Portland VA Medical Center Lines: 6 Message-ID: <463gma$2bk@maureen.teleport.com> NNTP-Posting-Host: ip-pdx08-58.teleport.com Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.6 Xref: biosci bionet.molbio.proteins.7tms_r:477 bionet.software:13649 I am looking for advice on software for PC/Windows that can carry out simultaneous nonlinear regression analysis of multiple curves - that is, the ability to determine shared values for multiple sets of data by fitting to a user-defined function. Freeware or shareware is preferred. Any suggestions? From owner-7tms_r@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!news.sprintlink.net!newsfeed.internetmci.com!psgrain!nntp.teleport.com!ip-pdx05-47.teleport.com!user From: wolop@teleport.com () Newsgroups: bionet.molbio.proteins.7tms_r Subject: Peptide and Small Molecule Libraries Followup-To: bionet.molbio.proteins.7tms_r Date: Wed, 18 Oct 1995 12:35:46 -0700 Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 5 Message-ID: NNTP-Posting-Host: ip-pdx05-47.teleport.com We are a West Coast biotechnology company seeking peptide and small molecule libraries to screen g-protein linked receptors. We would prefer to establish a collaborative effort with providers of such compounds but may be willing to purchase or lease if necessary. Please e-mail or telephone Paul Woloshin 503-243-6422 From owner-7tms_r@net.bio.net Sat Oct 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.luc.edu!orion.it.luc.edu!scukier From: scukier@orion.it.luc.edu (Sam Cukierman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: PKA+PKC Date: 22 Oct 1995 13:03:45 GMT Organization: Loyola University Chicago Lines: 5 Message-ID: <46dffh$kv1@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: scukier X-Newsreader: TIN [version 1.2 PL2] Could anyone recommend me a critical review on consensus sites for PKA and PKC? I also want to learn about specificity of these sites to a given kinase? Many thanks. Sam From owner-7tms_r@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU (Bryan Roth) Newsgroups: bionet.molbio.proteins.7tms_r Subject: molecular biology position Date: 23 Oct 1995 11:10:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510231808.SAA13846@biocserver.BIOC.CWRU.Edu> A position is available on January 1 aimed at elucidating how drugs bind to serotonin receptors using techniques of molecular biology (e.g. site-directed mutagenesis). Salary is negotiable depending upon experience. ============================================================= Bryan L.Roth Department of Biochemistry Room W438 CWRU Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 roth@biocserver.cwru.edu 216-368-2730 (Office) 216-368-4544 (FAX) From owner-7tms_r@net.bio.net Mon Oct 23 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sesqui.net!rice!wintermute.rice.edu!user From: jdb@bioc.rice.edu (John Bishop) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Kinetics questions Followup-To: bionet.molbio.proteins.7tms_r Date: 24 Oct 1995 20:22:20 GMT Organization: Rice University Lines: 18 Distribution: world Message-ID: NNTP-Posting-Host: wintermute.rice.edu I have a couple of general questions that you all might give me a consensus on 1. Does there seem to be a range of values around which dissociation constants for 7tmsr with polypeptide ligands cluster? 2. Are there examples of 7tmsr that don't downregulate in response to constant excess ligand? 3. What might be the functional implications of having a ligand-receptor complex with an unusually long association half-life? Thanks for any input. jdb@bioc.rice.edu From owner-7tms_r@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!CBRC.MGH.HARVARD.EDU!Ethan_Lerner From: Ethan_Lerner@CBRC.MGH.HARVARD.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: ligand homologies Date: 25 Oct 1995 12:34:27 -0700 Organization: CBRC, Massachusetts General Hospital Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <1995Oct25.115550.2897466950@cbrc.mgh.harvard.edu> NNTP-Posting-Host: net.bio.net Subject: Time:11:34 AM OFFICE MEMO ligand homologies Date:10/25/95 Are there examples of structurally unrelated ligands (at least on a primary sequence level) particularly when the ligands are peptides/proteins, where each has potent agonist activity and can compete with the other for binding to a G-protein coupled receptor? Thus, the binding of interleukin-2 and 15 to the IL-2 receptor satisfies the peptide/protein ligand part of the equation but not the receptor. The reverse is true for morphine and the endogenous opioid peptides. Thanks for the help. Ethan Lerner e-mail: ethan_lerner@cbrc.mgh.harvard.edu From owner-7tms_r@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!sol.ctr.columbia.edu!news.uoregon.edu!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.sesqui.net!rice!wintermute.rice.edu!user From: jdb@bioc.rice.edu (John Bishop) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Kinetics questions Followup-To: bionet.molbio.proteins.7tms_r Date: 24 Oct 1995 22:55:27 GMT Organization: Rice University Lines: 8 Distribution: world Message-ID: References: NNTP-Posting-Host: wintermute.rice.edu I mistyped #3 which should have been: 3. What might be the functional implications of having a ligand-receptor complex with an unusually long dissociation half-life? Thanks. From owner-7tms_r@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!novo.dk!byw From: byw@novo.dk (Robert Bywater) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 26 Oct 1995 00:03:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510260700.JAA15829@bach> NNTP-Posting-Host: net.bio.net Ethan Lerner writes ( Oct. 25 ) : >Are there examples of structurally unrelated ligands (at least on a primary >sequence level) particularly when the ligands are peptides/proteins, where >each has potent agonist activity and can compete with the other for binding >to a G-protein coupled receptor? I hope Dr. Lerner will summarize and disseminate the replies. This is one of the most important questions, not only in the GPCR field but throughout medicinal chemistry. In the case of GPCRs which have peptide ( and therefore very flexible ) ligands, the problem becomes most acute. NB. Of course there is really no meaning in the expression 'structurally unrelated' as applied here - if two ligands have identical biological activity then at the level of the wavefunction, which is the most accurate level at which structure and molecular similarity can be considered, they must be more or less identical, regardless of the particular molecular skeleton from which that wavefunction is derived. Robert Bywater ****************************** * * * Robert Bywater * * * * Biostructure Department * * NOVO NORDISK A/S * * * * DK-2880 BAGSVAERD Denmark * * * * email byw@novo.dk * * fax :: +45 4442 1400 * ****************************** From owner-7tms_r@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!INFOBAHNOS.COM!durocher From: durocher@INFOBAHNOS.COM (Yves Durocher) Newsgroups: bionet.molbio.proteins.7tms_r Subject: human G-alpha-S cDNA Date: 31 Oct 1995 18:35:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for an eucaryotic expression vector for the human G protein alpha-S. Please contact me if you know someone who has it. Thanks Yves From owner-7tms_r@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!rutgers!goliath.montclair.edu!newsserver.jvnc.net!news.merck.com!internal.merck.com!user From: Doug_MacNeil@merck.com (Douglas MacNeil) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: ligand homologies Followup-To: bionet.molbio.proteins.7tms_r Date: Wed, 01 Nov 1995 16:33:00 -0500 Organization: Merck Rsearch Labs Lines: 26 Distribution: world Message-ID: References: <1995Oct25.115550.2897466950@cbrc.mgh.harvard.edu> Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit One possible example of a GPCR with two unrelated ligands maybe the melanocortin receptor. In mouse, aMSH and the agouti protein are potent agonists of MCR1. Both ligands are competitive with each other. Doug MacNeil Merck Research Labs Rahway, NJ In article <1995Oct25.115550.2897466950@cbrc.mgh.harvard.edu>, Ethan_Lerner@CBRC.MGH.HARVARD.EDU wrote: > Subject: Time:11:34 AM > OFFICE MEMO ligand homologies Date:10/25/95 > Are there examples of structurally unrelated ligands (at least on a primary > sequence level) particularly when the ligands are peptides/proteins, where > each has potent agonist activity and can compete with the other for binding > to a G-protein coupled receptor? Thus, the binding of interleukin-2 and 15 > to the IL-2 receptor satisfies the peptide/protein ligand part of the > equation but not the receptor. The reverse is true for morphine and the > endogenous opioid peptides. Thanks for the help. Ethan Lerner > e-mail: ethan_lerner@cbrc.mgh.harvard.edu The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender.