From owner-7tms_r@net.bio.net Thu Feb 01 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!Germany.EU.net!Dortmund.Germany.EU.net!nntp.gmd.de!news.ruhr-uni-bochum.de!news.uni-stuttgart.de!uni-regensburg.de!lrz-muenchen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Expression in bacteria Date: 30 Jan 1996 17:59:16 GMT Organization: University of Wuerzburg, Germany Lines: 33 Distribution: world Message-ID: <4elm9k$ke4@winx03.informatik.uni-wuerzburg.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] BARRY J MARGULIES (bjmarg@WELCHLINK.WELCH.JHU.EDU) wrote: > Hi, again, folks. I've been told that bacterial expression is a > notoriously poor recombinant system for mammalian GCRs (i.e., bacteria > have a difficult time making these proteins). My p.i. would like a > reference to this, besides "word of mouth," for an upcoming publication. > Any help? @article{schertler:92a, author = {Gebhard F. X. Schertler}, title = {Overproduction of membrane proteins.}, journal = {Curr. Op. Struct. Biol.}, volume = 2, pages = {534--544}, year = 1992, } @article{grisshammer:95, author = {R. Grisshammer and C. G. Tate}, title = {Overexpression of integral membrane proteins for structural studies.}, journal = {Q. Rev. Biophys.}, volume = 28, number = 3, pages = {315--422}, year = 1995 } --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Feb 01 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rsesh@aol.com (RSesh) Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-docs or entry level positions Date: 2 Feb 1996 19:07:02 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <4eu8v6$17u@newsbf02.news.aol.com> Reply-To: rsesh@aol.com (RSesh) NNTP-Posting-Host: newsbf02.mail.aol.com Hi! I am looking for industrial/academic post-docs in the field of protein chemistry -structure, stability, function/folding studies in the San Francisco Bay area. I will be graduating in June'96. Sangita From owner-7tms_r@net.bio.net Fri Feb 02 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!sun4nl!news.nic.surfnet.nl!surfnet.nl!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 2D and 3D structure of GPCR ligands Date: 1 Feb 1996 08:54:52 GMT Organization: University of Wuerzburg, Germany Lines: 45 Distribution: world Message-ID: <4epv4s$jim@winx03.informatik.uni-wuerzburg.de> References: <01I0NQZLRJPKA0URL2@mbcl.rutgers.edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] WCUI@OCELOT.RUTGERS.EDU wrote: > I like to find sources for 2D and 3D structures of GPCR ligands. It's difficult to answer this question because the GPCR ligands are so diverse. Traditionally structures of "small" and "large" molecules are stored in different databases. The 3D structures of small molecules, such as isoproterenol, might be available in the Cambridge Structural Database. I do not know of any URL and believe that the CSD is only accessible for $$$. Some of the structures might also be available in the Klotho database at http://ibc.wustl.edu/klotho/ The 3D structures of large ligands such as thrombin can be found in the PDB; have a look at http://www.pdb.bnl.gov and http://molbio.info.nih.gov/cgi-bin/pdb ("Molecules R Us", a full-text search facility of the PDB) As for 2D structures, for proteins there is always PIR or SWISSPROT; I have no idea if there is a database of structural formulae for "small" ligands. It would certainly be nice to have an integrated database of GPCR ligands, however, I think it might be advantageous if this were part of one of the two already existing GPCR databases at http://receptor.mgh.harvard.edu/GCRDBHOME.html http://www.sander.embl-heidelberg.de/7tm/ (these sites are maintained by Lee Kolakowski and Gert Vriend, respectively). Hope that helps, --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!COURRIER.USHERB.CA!mrolaple From: mrolaple@COURRIER.USHERB.CA (Marek Rola-Pleszczynski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 2D and 3D structure of GPCR ligands Date: 5 Feb 1996 09:33:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Have you seen the NMR-derived 3-D structures of two GPCR ligands, RANTES and interleukin-8, in J. Leuk. Biol., January 1996 issue? Cheers ******************* Marek Rola-Pleszczynski,M.D. Immunologie Faculte de Medecine Universite de Sherbrooke 3001 12e Avenue N. Sherbrooke, QC J1H 5N4 Canada From owner-7tms_r@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!gossip.pyramid.com!news.sedona.net!usenet From: Ann Rathbun Newsgroups: bionet.molbio.proteins.7tms_r Subject: Analytical Biochem Dev't Manager Date: 5 Feb 1996 19:15:33 GMT Organization: Sedona Internet Services, Inc. Lines: 52 Message-ID: <4f5l0l$6ku@labrat.sedona.net> NNTP-Posting-Host: client8.sedona.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Manager, Analytical Biochemistry Development We are seeking a Manager for a newly formed Methods Development group with our biopharmaceutical client company on the East Coast. The successful Ph.D.-level Protein Biochemist will be responsible for methods development for the characterization and evaluation of [protein] products and processes for our biopharmaceutical client company. Candidates must have exceptionally strong communication skills as he/she will be the key link with the company’s foreign collaborators and will be responsible for putting forth ideas and plans to implement their various projects. Skills/Background: · Ph.D. in Biochemistry, Analytical Chemistry or related discipline; · Analytical methods development for proteins, esp. recombinant; · Strong Mass Spec experience with is a requirement; · 4 plus years postdoc with supervisory [hiring, promotion and firing responsibilities] experience; · Industry experience is preferred; · Ideally this person will have GMP and Regulatory filing experience. Other Desired Skills: Amino Acid Analysis Bioassay Chromatography Circular Dichroism Electrophoresis HPLC Sequencing, N- & C- terminal Structure/Function Tryptic Mapping If you have an interest in this or other opportunities, please mail or FAX your CV/resume to RS&A to the attention of Ann G. Rathbun. All correspondence is held in strict confidence. Ann Rathbun Managing Director Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520) 284-3361 FAX E-mail: rathbun@ sedona.net From owner-7tms_r@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!BIOBASE.DK!thorup From: thorup@BIOBASE.DK (Jan Ulrik Thorup) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 11th International Symposium on Regulatory Peptides Date: 6 Feb 1996 05:29:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net 11th International Symposium on Regulatory Peptides (Formerly Gastrointestinal Hormones) Copenhagen, Denmark September 3-7, 1996 ------------------------------------------------------------------------------- The Symposium will discuss hormones, neuropeptides and paracrine peptides from molecular and cell biology, secretion, receptors and pharmacology to their role in cancer, neuropsychiatric, gastrointestinal and endocrine disorders. Plenary speakers: Sir James Black, London (Heptahelix receptors) Jeffrey M. Friedman, New York (Peptides and obesity) Carl-Henrik Heldin, Uppsala (PDGF and TGFb in growth control) Wieland Huttner,Heidelberg(Secretory granules in neuroendocrine cells) Gilbert Vassart, Brussels (Receptor mutants and disease) In addition 150 shorter oral and 300 poster presentations will be selected from submitted abstracts (deadline April 1, 1996) ------------------------------------------------------------------------------- Organizers: Information and registration: Jens F. Rehfeld Symposium on Regulatory Peptides Linda Hilsted c/o DIS Congress Service Copenhagen Linda Bardram Herlev Ringvej 2C Jan Fahrenkrug DK-2730 Herlev, Denmark Jens Juul Holst Phone;+45 44 92 44 92 Cathrine Oerskov Fax;+45 44 92 50 50 From owner-7tms_r@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!csn!nntp-xfer-2.csn.net!csn!ub!dsinc!netnews.upenn.edu!brass.med.upenn.edu!user From: brass@mail.med.upenn.edu (Skip Brass) Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-doc positions available Date: Tue, 06 Feb 1996 13:29:50 -0500 Organization: University of Pennsylvania Lines: 14 Message-ID: NNTP-Posting-Host: brass.med.upenn.edu Two NIH-funded post-doctoral are available in my lab at the University of Pennsylvania in Philadelphia. The projects focus on signal transduction events in platelets and endothelial cells, and deal primarily with G proteins and G protein coupled receptors. Molecular biology experience necessary. Transgenic mouse experience desirable for one of the positions. Contact me by e-mail for additional information. You can also e-mail or FAX me your CV. Skip Brass Dept of Medicine University of Pennsylvania brass@mail.med.upenn.edu FAX: 215-573-2189 From owner-7tms_r@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!tcrl.jti.co.jp!hirano From: hirano@tcrl.jti.co.jp ("Daisuke Hirano") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Searching for cell line Date: 6 Feb 1996 16:57:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <9602070057.AA16216@ns.tcrl.jti.co.jp> NNTP-Posting-Host: net.bio.net Searching for cell line Does anyone give me a "murine" endotherial cell line? I searched some cell banks, but could not find it. Daisuke Hirano Japan Tobacco Inc. Life Science Research Laboratory hirano@life.tcrl.jti.co.jp FAX:81-45-972-6205 From owner-7tms_r@net.bio.net Tue Feb 06 22:00:00 1996 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!lhc.nlm.nih.gov!crick.sura.net!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!news From: Dave Rominger Subject: Thue Schwartz email Content-Type: text/plain; charset=us-ascii Message-ID: <3118B030.43BC@a1.lldmpc.umc.dupont.com> Sender: news@es.dupont.com (USENET News System) Nntp-Posting-Host: esm155120.dmpc.com Content-Transfer-Encoding: 7bit Organization: DMPC Mime-Version: 1.0 Date: Wed, 7 Feb 1996 13:59:12 GMT X-Mailer: Mozilla 2.0b6a (Macintosh; I; PPC) Lines: 10 Does anyone know the email adress for Thue Schwartz? I could send a postal letter but would prefer an email. Dave _________________________________________________________ David Rominger DuPont Merck Pharmaceuticals romingdh@a1.lldmpc.umc.dupont.com Phone (302) 695-7142 FAX (302) 695-3730 From owner-7tms_r@net.bio.net Tue Feb 06 22:00:00 1996 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!lhc.nlm.nih.gov!crick.sura.net!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!news From: Dave Rominger Subject: LIGAND receptor Analysis Content-Type: text/plain; charset=us-ascii Message-ID: <3118CE04.296F@a1.lldmpc.umc.dupont.com> Sender: news@es.dupont.com (USENET News System) Nntp-Posting-Host: esm155120.dmpc.com Content-Transfer-Encoding: 7bit Cc: bionet.molec-model Organization: DMPC Mime-Version: 1.0 Date: Wed, 7 Feb 1996 16:06:28 GMT X-Mailer: Mozilla 2.0b6a (Macintosh; I; PPC) Lines: 17 We have been wroking with LIGAND for Macintosh. We are currently MAC based. Since the emergence of Powermac's the version of LIGAND from NIH no longer is written to run on a powerMac. Could someone offer a solution for LIGAND non-linear anlaysis similar to LIGAND, that will run on the powerMAC. Any suggestions, solutions would be appreciated. Dave _________________________________________________________ David Rominger DuPont Merck Pharmaceuticals romingdh@a1.lldmpc.umc.dupont.com Phone (302) 695-7142 FAX (302) 695-3730 From owner-7tms_r@net.bio.net Tue Feb 06 22:00:00 1996 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!lhc.nlm.nih.gov!crick.sura.net!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!news From: Dave Rominger Subject: Thue Schwartz email Content-Type: text/plain; charset=us-ascii Message-ID: <3118AFBF.120B@a1.lldmpc.umc.dupont.com> Sender: news@es.dupont.com (USENET News System) Nntp-Posting-Host: esm155120.dmpc.com Content-Transfer-Encoding: 7bit Organization: DMPC Mime-Version: 1.0 Date: Wed, 7 Feb 1996 13:57:19 GMT X-Mailer: Mozilla 2.0b6a (Macintosh; I; PPC) Lines: 10 Does anyone know the email adress for Thue Schwartz? I could send a postal letter but would prefer an email. Dave _________________________________________________________ David Rominger DuPont Merck Pharmaceuticals romingdh@a1.lldmpc.umc.dupont.com Phone (302) 695-7142 FAX (302) 695-3730 From owner-7tms_r@net.bio.net Tue Feb 06 22:00:00 1996 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!lhc.nlm.nih.gov!crick.sura.net!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!news From: Dave Rominger Subject: Thue Schwartz email Content-Type: text/plain; charset=us-ascii Message-ID: <3118B0A0.1B85@a1.lldmpc.umc.dupont.com> Sender: news@es.dupont.com (USENET News System) Nntp-Posting-Host: esm155120.dmpc.com Content-Transfer-Encoding: 7bit Organization: DMPC Mime-Version: 1.0 Date: Wed, 7 Feb 1996 14:01:04 GMT X-Mailer: Mozilla 2.0b6a (Macintosh; I; PPC) Lines: 10 Does anyone know the email adress for Thue Schwartz? I could send a postal letter but would prefer an email. Dave _________________________________________________________ David Rominger DuPont Merck Pharmaceuticals romingdh@a1.lldmpc.umc.dupont.com Phone (302) 695-7142 FAX (302) 695-3730 From owner-7tms_r@net.bio.net Tue Feb 06 22:00:00 1996 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Job Listing (Fyn Kinases work in Montreal... PhD. position) Date: 7 Feb 1996 18:57:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <31196372.2817@odyssee.net> NNTP-Posting-Host: net.bio.net Job Listing #2: Characterization of The RNA Binding Ability of SAM68 Dr.Stéphan Richard, Ph.D Lady Davis Institute for Medical Research Montreal, Quebec, Canada Project description: The focus of our laboratory is on signal transduction mediated by tyrosine kinases. We have previously discovered Sam68 which is a fyn, src family tyrosine kinase which binds SH3 domains. Further we have demonstrated that many other proteins with SH3 and SH2 domains bind Sam68 including ras-GTPase activating protein (GAP), phospholipase C-1 and Grb2 (Mol. Cell. Biol. 1995, 15:186). Sam68 defines a family of proteins that bind signal transduction molecules and RNA. Interestingly, phosphorylation of this protein abolishes its ability to bind RNA(J. Biol. Chem. 1995, 270:2010). Specifically the aims of the project are: 1.to delineate the RNA binding domains of Sam68 and to understand the mechanism by which phosphorylation abolishes RNA binding. 2.to identify physiologcal targets for the RNA binding daomain of Sam68. The student will gain knowledge in signal transduction, as well as techniques in molecular biology, cell culture, phosphorylation and immunology. Positions: Ph.D. Postion -to qualify, applicants must have a B.Sc. in molecular biology, biochemistry or immunology in addition to obtaining a GPA of greater than 3.4. To Apply: Please send your curriculum vitae and a copy of your transcript to: Dr. Stéphan Richard Lady Davis Institute 3755 Cote St. Catherine Road Montreal, Quebec Canada H3T 1E2 mcrd@musica.mcgill.ca (for more jobs in the Montreal Area try our web site....) http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html From owner-7tms_r@net.bio.net Wed Feb 07 22:00:00 1996 Path: biosci!UOFT02.UTOLEDO.EDU!wmesser From: wmesser@UOFT02.UTOLEDO.EDU (Bill Messer) Newsgroups: bionet.molbio.proteins.7tms_r Subject: postdoctoral fellowship available Date: 8 Feb 1996 13:38:34 -0800 Organization: The University of Toledo Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I0Z6YTTG2W00PW7S@UOFT02.UTOLEDO.EDU> NNTP-Posting-Host: net.bio.net Neurobiology of Muscarinic Receptor Subtypes A Postdoctoral Fellowship is available to study the biological=20 consequences of ligand selectivity and receptor activity from the= =20 molecular level to the whole animal. The position is funded from an = NIH=20 grant aimed at the development of novel and selective muscarinic=20 agonists for the treatment of Alzheimer=B9s disease. =20 A Ph.D. in Neuroscience, Pharmacology, Biochemistry or related field = is=20 required. Experience with signal transduction mechanisms,=20 neurotransmitter pharmacology and molecular biology is desirable. Th= e=20 position is available on April 1, 1996 with a starting salary of=20 $25,725. Letters of interest and references should be sent to William S. Messe= r,=20 Jr., Ph.D., Department of Medicinal and Biological Chemistry, College= of=20 Pharmacy, The University of Toledo, 2801 W. Bancroft St., Toledo, OH = =20 43606. Email inquiries are also welcome at wmesser@uoft02.utoledo.ed= u. From owner-7tms_r@net.bio.net Thu Feb 08 22:00:00 1996 Path: biosci!BIOCELL.IRMKANT.RM.CNR.IT!7tm From: 7tm@BIOCELL.IRMKANT.RM.CNR.IT (Transmembrane receptors) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Lysine in 2nd position of DRY-like motif? Date: 9 Feb 1996 08:21:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On Jan 30, 1996, Ken Rice wrote: >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> The DRY motif in the rhodopsin-beta2 family is seen in several variations: DRC, DRF, DRH, ERC, etc. Is anyone aware of a member of the family that has a lysine in the second position of the motif, e.g. DKY, EKC, or whatever? Thanks, Ken Rice >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Adding to K.Rice question: Are the 7TM receptors containing the DRF or ERF variants of the DRY motif, sharing other structural/functional properties? Thanks everybody, Raffaele Matteoni Inst of Cell Biology Natl Res Council I-00137 Roma Italy ------- From owner-7tms_r@net.bio.net Thu Feb 08 22:00:00 1996 Path: biosci!MAX.MPIBP.UNI-FRANKFURT.DE!teeter From: teeter@MAX.MPIBP.UNI-FRANKFURT.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 2D and 3D structure of GPCR ligands Date: 9 Feb 1996 03:17:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <0099DA6A.E0490240.25374@max.mpibp.uni-frankfurt.de> NNTP-Posting-Host: net.bio.net WCUI@OCELOT.RUTGERS.EDU wrote: > > I like to find sources for 2D and 3D structures of GPCR ligands. > Is there already a database that contains such data? There is an Atlas of the Three-Dimensional Structure of Drugs by Tollenaere, Moereels and Raymaekers. Many of these are imporatant GPCR ligands. Although this book is quite old (1979), so are many of the crsytal structures of important small molecule GPCR ligands. The authors may have coordinates in-house extracted from various data bases that could become the basis of such a data base. The book contains pictures of the drugs, their formulas and references to their activities and structures (nearly all crystallographic). It is to be a bridge between the structural chemists and the pharmacologists. > if so, where are they and how can I get them? The book is copywrited to Janseen Research Foundation and is published by Elsevier/North-Holland Biomedical Press. > if not, where should I start if I want build such database? > Any suggestions will be fully appreciated. > > Weili Cui, Ph.D. > Senior Scientist > Synaptic Pharmaceutical Corp. --------------------------------------------------------------------- Martha M. Teeter From owner-7tms_r@net.bio.net Thu Feb 08 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-lyon1.fr!news-rocq.inria.fr!news2.EUnet.fr!EU.net!howland.reston.ans.net!newsxfer.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: LIGAND receptor Analysis Date: Thu, 08 Feb 1996 14:36:46 -0500 Organization: University of Michigan Lines: 30 Message-ID: <311A50CE.5AEF@umich.edu> References: <3118CE04.296F@a1.lldmpc.umc.dupont.com> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b6a (Win95; I) CC: Harvey Motulsky Dave, What specific features of LIGAND are you looking for? Rick Dave Rominger wrote: > > We have been wroking with LIGAND for Macintosh. We are currently MAC > based. Since the emergence of Powermac's the version of LIGAND from > NIH no longer is written to run on a powerMac. > > Could someone offer a solution for LIGAND non-linear anlaysis similar > to LIGAND, that will run on the powerMAC. > > Any suggestions, solutions would be appreciated. > > Dave > > _________________________________________________________ > David Rominger > DuPont Merck Pharmaceuticals > romingdh@a1.lldmpc.umc.dupont.com > Phone (302) 695-7142 > FAX (302) 695-3730 -- _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 From owner-7tms_r@net.bio.net Sun Feb 11 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 12 Feb 1996 02:00:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602121000.CAA27567@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Sun Feb 11 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!newsfeed.internetmci.com!portal.gmu.edu!hearst.acc.Virginia.EDU!gems.vcu.edu!vshowalter From: vshowalter@gems.vcu.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: re: LIGAND receptor Analysis Message-ID: <1996Feb12.114655.2051@gems.vcu.edu> Date: 12 Feb 96 11:46:55 -0400 Organization: Medical College of Virginia Lines: 26 >From: Dave Rominger >Subject:LIGAND receptor Analysis >Date: Wed, 7 Feb 1996 16:06:28 GMT >Message-ID:<3118CE04.296F@a1.lldmpc.umc.dupont.com> >We have been wroking with LIGAND for Macintosh. We are currently MAC >based. Since the emergence of Powermac's the version of LIGAND from >NIH no longer is written to run on a powerMac. > >Could someone offer a solution for LIGAND non-linear anlaysis similar >to LIGAND, that will run on the powerMAC. > >Any suggestions, solutions would be appreciated. > >Dave > >_________________________________________________________ >David Rominger >DuPont Merck Pharmaceuticals * >romingdh@a1.lldmpc.umc.dupont.com * >Phone (302) 695-7142 >FAX (302) 695-3730 Actually, EBDA and LIGAND will run on a PowerMac. You need to turn the Modern memory manger off and restart with extensions off in order to run it. This worked for us. If it doesn't for you, call Apple. From owner-7tms_r@net.bio.net Mon Feb 12 22:00:00 1996 Path: biosci!THALAMUS.WUSTL.EDU!brodbecr From: brodbecr@THALAMUS.WUSTL.EDU (Robbin Brodbeck) Newsgroups: bionet.molbio.proteins.7tms_r Subject: More about LIGAND Date: 13 Feb 1996 05:49:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602131346.HAA06974@thalamus.wustl.edu> NNTP-Posting-Host: net.bio.net Dear 7TMers: We've been working for many years with the PC version of LIGAND, and would like to purchase the latest version of it for Macintosh...since that is now the primary computer in the lab, and our only PC has a mere 20MB HD. Can anyone point me in the right direction? Robbin Brodbeck ----------------------- Robbin M. Brodbeck, Ph.D. brodbecr@thalamus.wustl.edu Washington University School of Medicine 660 S. Euclid ave. Dept. of Anatomy and Neurobiology Box 8108 St. Louis, MO. 63110 (314) 362-3468 Fax (314) 362-3446 From owner-7tms_r@net.bio.net Mon Feb 12 22:00:00 1996 Path: biosci!THALAMUS.WUSTL.EDU!brodbecr From: brodbecr@THALAMUS.WUSTL.EDU (Robb Brodbeck) Newsgroups: bionet.molbio.proteins.7tms_r Subject: LIGAND Date: 13 Feb 1996 20:06:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602140403.WAA11411@thalamus.wustl.edu> NNTP-Posting-Host: net.bio.net The 7TM group has been very helpful in putting me in touch with the Biosoft company (PO Box 10938, Ferguson, MO 63135 Tel. (314) 524 8029, Fax. (314) 524 8129) which sells the LIGAND program for both Macs and PCs. I learned that a) Mac v.2.0 is currently available, and will run on a powerMac under the conditions previously discussed, b) the PC v.4.0 is a far superior program to the Mac v.2.0 and c) a new and much improved Mac version is a month or so away from release for ?$. I'm on the mailing list and will relay the information to the 7TM group as soon as more data is made available. Thanks for the help! Robb Robbin M. Brodbeck Ph.D. Washington University School of Medicine Dept. of Anatomy and Neurobiology Box 8118 660 S. Euclid Ave. St. Louis, MO 63110 (314) 362-3468 fax (314) 362-3446 brodbecr@thalamus.wustl.edu From owner-7tms_r@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Human beta-2 pdb file Date: 14 Feb 1996 08:42:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <960214.113929.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net Yes, I know there's no "real" structure, but does anyone know of a deposited PDB file or have one that they would be willing to email to me? Thanks mucho!! | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-1553 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O | From owner-7tms_r@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!MOOSE.UVM.EDU!tspaldin From: tspaldin@MOOSE.UVM.EDU ("Tracy A. Spalding") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Human beta-2 pdb file Date: 14 Feb 1996 16:15:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <960214.113929.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net The Swiss-model 7TM automated sequencing page uses a predicted B2-adrenergic receptor as a template http://expasy.hcuge.ch/cgi-bin/ProMod-GPCR.pl Has anyone else tried this service? I would be interested to hear about people's experiences with it. Yours, Tracy Spalding Department of Psychiatry University of Vermont Burlington VT 05405 (802) 656-2105 On 14 Feb 1996 RHODES@uconnvm.uconn.edu wrote: > Yes, I know there's no "real" structure, but does anyone know of > a deposited PDB file or have one that they would be willing to > email to me? Thanks mucho!! > > | O==O | > | DAVID G. RHODES O==O PHONE 860-486-5413 | > | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-1553 | > | UNIVERSITY OF CONNECTICUT O==O | > | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | > | O==O | > > From owner-7tms_r@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!NS.OSAKA.WEB.AD.JP!ktaka From: ktaka@NS.OSAKA.WEB.AD.JP (Kazuchika Takagaki) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 14 Feb 1996 21:01:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602150459.NAA25496@mb.osaka.infoweb.or.jp> NNTP-Posting-Host: net.bio.net subscribe 7tms_r From owner-7tms_r@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!BLUE.INCM.U-NANCY.FR!maigret From: maigret@BLUE.INCM.U-NANCY.FR Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Human beta-2 pdb file Date: 14 Feb 1996 10:17:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <960214.113929.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net Dear David, We have a model structure of human beta2-adrenergic GPCR (see Strosberg et al. Drug Design and Discovery, 1993, vol 9, 199-211). If you are interested in I can E-mail it in PDB or Biosym .car formats Bests Bernard Maigret Laboratory of Theoretical Chemistry University H. Poincare Nancy, Fr From owner-7tms_r@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.internetMCI.com!darwin.sura.net!maze.dpo.uab.edu!usenet From: Wayne Duck Newsgroups: bionet.molbio.proteins.7tms_r Subject: Dr. Jimmy Neill's GnRH Receptor Research Web Page Date: Wed, 14 Feb 1996 16:53:30 -0600 Organization: University of Alabama at Birmingham Lines: 22 Message-ID: <312267EA.4C55@phy164.physiology.uab.edu> NNTP-Posting-Host: neill.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Dear 7Tmers, Dr Jimmy D. Neill has a lab Web Page that has information on our research involving the GnRH Receptor. http://neill.physiology.uab.edu -- ---------------------------------------------------------- Wayne Duck "War Eagle" Research Assistant at UAB Dept. of Physiology and Biophysics guest2@phybio.bhs.uab.edu http://neill.physiology.uab.edu http://neill.physiology.uab.edu/wayne http://neill.physiology.uab.edu/wayne/auball.htm "Houston, Tranquility base here, The Eagle has landed." ---------------------------------------------------------- From owner-7tms_r@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Human beta-2 pdb file Date: 14 Feb 1996 17:27:45 GMT Organization: University of Wuerzburg, Germany Lines: 15 Distribution: world Message-ID: <4ft62h$823@winx03.informatik.uni-wuerzburg.de> References: <960214.113929.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] RHODES@UCONNVM.UCONN.EDU wrote: > Yes, I know there's no "real" structure, but does anyone know of > a deposited PDB file or have one that they would be willing to > email to me? Thanks mucho!! Check out the links at http://www.sander.embl-heidelberg.de/7tm/models/models.html --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Human beta-2 pdb file (fwd) Date: 15 Feb 1996 04:39:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <960215.073638.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net OK ! ! ! ! What a great group! Within a couple of hours I had 4 people point out the site at: http://www.sander.embl-heidelberg.de/7tm/models/models.html and more who offered their own editions. There were more this AM, and at least one person who wanted to know what I found out... Bottom line- If you haven't tried the page at Heidelberg, DO check it out!!! it is a tremendous resource... Once again - thanks to all who responded. }:) | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-1553 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O | From owner-7tms_r@net.bio.net Thu Feb 15 22:00:00 1996 Path: biosci!PT.CYANAMID.COM!hadcockj From: hadcockj@PT.CYANAMID.COM Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 16 Feb 1996 06:16:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I19XPG3CSECNRD7V@ptag2.pt.Cyanamid.COM> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Sun Feb 18 22:00:00 1996 Path: biosci!prius.jnj.com!gunnet From: gunnet@prius.jnj.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: (no subject) Date: 19 Feb 1996 10:42:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I1EDYY1XCY90NHR0@alloy.prius.jnj.com> NNTP-Posting-Host: net.bio.net I am looking for a cell line expressing high concentrations of the CGRP receptor. Is there one available or if not would anyone able to create a such a line in a timely fashion? From owner-7tms_r@net.bio.net Sun Feb 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!uwm.edu!msunews!netnews.upenn.edu!news.tju.edu!stubbs1.tjh.tju.edu!user From: yeagerm@jeflin.tju.edu (Mark Yeager) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: LIGAND Date: Mon, 19 Feb 1996 12:42:50 -0500 Organization: Thomas Jefferson University Lines: 13 Distribution: world Message-ID: References: <199602140403.WAA11411@thalamus.wustl.edu> NNTP-Posting-Host: stubbs1.tjh.tju.edu X-Newsreader: Yet Another NewsWatcher 2.2.0b4 In article <199602140403.WAA11411@thalamus.wustl.edu>, brodbecr@THALAMUS.WUSTL.EDU (Robb Brodbeck) wrote: > The 7TM group has been very helpful in putting me in touch with the Biosoft > company (PO Box 10938, Ferguson, MO 63135 Tel. (314) 524 8029, Fax. (314) > 524 8129) which sells the LIGAND program for both Macs and PCs. How can a private firm sell a program that was developed with public NIH funds? -- -- -.-- Mark Yeager -> yeagerm@jeflin.tju.edu Dept. Pathology and Cell Biology, Thomas Jefferson Univ., Phila., PA From owner-7tms_r@net.bio.net Sun Feb 18 22:00:00 1996 Path: biosci!ccmail.med.nyu.edu!ERIC.SIMON From: ERIC.SIMON@ccmail.med.nyu.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 19 Feb 1996 07:46:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I1E7WAZTDY8WW195@MCRCR6.MED.NYU.EDU> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!col.hp.com!csn!news-1.csn.net!magnus.acs.ohio-state.edu!news From: danforth.2@osu.edu (Douglas Danforth) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: LIGAND Date: Tue, 20 Feb 1996 14:37:01 GMT Organization: The Ohio State University Lines: 38 Message-ID: <4gcma9$mjb@charm.magnus.acs.ohio-state.edu> References: <199602140403.WAA11411@thalamus.wustl.edu> NNTP-Posting-Host: ddanforth.med.ohio-state.edu X-Newsreader: Forte Free Agent 1.0.82 yeagerm@jeflin.tju.edu (Mark Yeager) wrote: >In article <199602140403.WAA11411@thalamus.wustl.edu>, >brodbecr@THALAMUS.WUSTL.EDU (Robb Brodbeck) wrote: >> The 7TM group has been very helpful in putting me in touch with the Biosoft >> company (PO Box 10938, Ferguson, MO 63135 Tel. (314) 524 8029, Fax. (314) >> 524 8129) which sells the LIGAND program for both Macs and PCs. >How can a private firm sell a program that was developed with public NIH funds? >-- >-- -.-- >Mark Yeager -> yeagerm@jeflin.tju.edu >Dept. Pathology and Cell Biology, Thomas Jefferson Univ., Phila., PA Mark, I e-mailed NIH directly requesting information on LIGAND. Here is their reply. Doug Danforth danforth.2@osu.edu Mr. Danforth, Ligand is available for a small handling fee. Contact Arnie Cushing at (301) 585-0515 x2005. If you have any problems getting it, let us know. Steve Noe TASC CSB/DCRT From owner-7tms_r@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsserver.jvnc.net!crick.bms.com!NewsWatcher!user From: watson_j@bms.com (A. John Watson) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Epitope Tagging Suggestions Wanted Date: Fri, 16 Feb 1996 11:46:08 -0300 Organization: Bristol-Myers Squibb Lines: 20 Message-ID: NNTP-Posting-Host: 140.176.107.223 Xref: biosci bionet.molbio.methds-reagnts:40646 bionet.molbio.proteins.7tms_r:623 I am planning to epitope tag one of my favorite receptors and am wondering what systems have people found to be particularly reliable/unreliable with respect to (a) choice of tag sequence and (b) source of anti-tag antibody. I am aware of Brian Kobilka's Anal. Biochem. paper recommending the FLAG tag and anti-FLAG antisera from Eastman Kodak. All responses appreciated. Will post tabulated responses for the dining and dancing pleasure of all AJW. ---------------- John Watson Bristol-Myers Squibb Co. watson_j@bms.com --------------------------------------------------------------------- "If you're not part of the solution, you're part of the precipitate." --------------------------------------------------------------------- From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!sunsite.doc.ic.ac.uk!ukc!news From: David Mac Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Human beta-2 pdb file Date: 21 Feb 1996 11:42:18 GMT Organization: University of Kent Lines: 3 Message-ID: <4gf0eq$63g@rook.ukc.ac.uk> References: <960214.113929.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: pcbi015.ukc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: David,G.,RHODES You can obtain a model from GPCRDB (Dan Donelleys) model of the Human beta-2 receptor. From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!SEQAXP.BIO.CALTECH.EDU!VSLEPAK From: VSLEPAK@SEQAXP.BIO.CALTECH.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Feb 1996 09:36:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <960221093422.2021ee8b@seqaxp.bio.caltech.edu> NNTP-Posting-Host: net.bio.net Unsubscribe From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!chempath.uct.ac.za!FLANAGAN From: FLANAGAN@chempath.uct.ac.za ("Flanagan, C, Colleen, Dr") Newsgroups: bionet.molbio.proteins.7tms_r Subject: POST-DOCTORAL POSITION AVAILABLE Date: 21 Feb 1996 00:34:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITION AVAILABLE A position is available to investigate receptor-ligand interactions in the G protein-coupled receptor for the peptide hormone, gonadotropin releasing hormone (GnRH). Our research program involves multiple aspects of GnRH and the GnRH receptor including identifying contact sites of both agonist and antagonist ligands, development of novel ligands for clinical and veterinary applications, receptor activation and coupling to G-proteins and intracellular signal transduction, and regulation of receptor gene expression. Candidates should have a PhD (or equivalent degree). Research experience in expression of recombinant proteins, analysis of receptor-ligand interactions or protein chemistry would be desirable. The Regulatory Peptides Research Unit provides a strong research environment with emphasis on international collaboration and publication. The city of Cape Town offers the many cultural activities associated with a large cosmopolitan city, in addition to a mild climate and a unique outdoor life-style. Send applications, including CV, to: Prof. Robert P. Millar Regulatory Peptides Research Unit Department of Chemical Pathology University of Cape Town Medical School Observatory 7925, South Africa. or EMail: Millar@chempath.uct.ac.za or fax: +27-21-4066153 For more information, use one of the above addresses or telephone Prof Millar, who is currently in Seattle, at +1-206- 4426627 or fax him at +1-206-4426608. Interviews will commence early in March, but the position will remain open until a suitable candidate is found. Relevant recent publications include: 1. Sealfon, S. C. and Millar, R. P., Functional Domains of the Gonadotropin Releasing Hormone Receptor, Cell. Mol. Neurobiol. 15, 25-42 (1995). 2. Flangan, C.A., Becker, I.I, Davidson, J. S., Wakefield, I. K., Zhou, W., Sealfon, S. C. and Millar, R. P., Glutamate301 of the Mouse Gonadotropin Releasing Hormone Receptor Confers Specificity for Arginine8 of Mammalaian Gonadotropin Releasing Hormone, J. Biol. Chem. 269, 22636-22641 (1994). From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!MEDMIC.RULIMBURG.NL!P.Beisser From: P.Beisser@MEDMIC.RULIMBURG.NL ("Patrick Beisser, University of Limburg, The Netherlands") Newsgroups: bionet.molbio.proteins.7tms_r Subject: rat fibroblast like cell line Date: 21 Feb 1996 03:57:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I1H4QYY0AQ000XJQ@RL0002.RULIMBURG.NL> NNTP-Posting-Host: net.bio.net Dear 7tms_rs, Is there anyone among you who has a rat fibroblast like cell line? We are fed up with the fresh isolates from rat embryos we use for propagation of rat cytomegalovirus. If you have, please respond to: Patrick Beisser, dept. of Medical Microbiology, University of Maastricht, the Netherlands. P.O. Box 5800, 6202 AZ Maastricht, the Netherlands. E-mail: P.Beisser@MEDMIC.RULimburg.NL fax: --31 433876643 From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!sbphrd.com!Jonathan_A_Lee From: Jonathan_A_Lee@sbphrd.com (Jonathan A Lee) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Ligand Analysis Date: 21 Feb 1996 12:39:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <9602212341.AA1777@pho903.sbphrd.com> NNTP-Posting-Host: net.bio.net 7TMers, We have been using the EBDA package for fitting our competition data. Are there any programs (PC or MAC) that can utilize an EXCEL spreadsheet as an input file? Thanks. Jonathan A. Lee, Ph.D. SmithKline Beecham Pharmaceuticals Dept. Macromolecular Science Mail Code UE 0447 709 Swedeland Road King of Prussia, PA 19406 TEL: 610-270-7588 FAX: 610-270-4091 Email: Jonathan_A_Lee@sbphrd.com From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!WELCHLINK.WELCH.JHU.EDU!jmilne From: jmilne@WELCHLINK.WELCH.JHU.EDU (JACQUELINE L MILNE) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Feb 1996 15:25:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!NEWSSUN.MED.MIAMI.EDU!vslepak From: vslepak@NEWSSUN.MED.MIAMI.EDU ("Vladlen Z. Slepak") Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe from new address Date: 21 Feb 1996 09:42:46 -0800 Organization: University of Miami Medical School Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <312B594F.69F2@newssun.med.miami.edu> Reply-To: vslepak@newssun.med.miami.edu NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Tue Feb 20 22:00:00 1996 Path: biosci!ISERVER.ICON.PALO-ALTO.MED.VA.GOV!bbh From: bbh@ISERVER.ICON.PALO-ALTO.MED.VA.GOV (Brian Hoffman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Feb 1996 10:15:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!ccmail.med.nyu.edu!ERIC.SIMON From: ERIC.SIMON@ccmail.med.nyu.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 22 Feb 1996 07:08:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I1IDFNIUVS8WW7SX@MCRCR6.MED.NYU.EDU> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.internetMCI.com!darwin.sura.net!maze.dpo.uab.edu!usenet From: Wayne Duck Newsgroups: bionet.molbio.proteins.7tms_r Subject: Phosphorylation of 7tmrs Date: Thu, 22 Feb 1996 16:41:35 -0600 Organization: University of Alabama at Birmingham Lines: 16 Message-ID: <312CF11F.2DC0@phy164.physiology.uab.edu> NNTP-Posting-Host: neill.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) We are about to attempt phosphorylation studies of an epitope-tagged GnRH receptor in transfected cells. Protocols call for high levels (2mCi/ml) of P32 for short times (2hrs) or low levels (100uCi/ml) for extended times (18hrs). Which is best for 7tmrs? -- ---------------------------------------------------------- Wayne Duck Dr. Jimmy D. Neill's Lab Dept. of Physiology and Biophysics mailto:guest2@phybio.bhs.uab.edu mailto:wayne-duck@phy164.physiology.uab.edu http://neill.physiology.uab.edu "Houston, Tranquility base here, The Eagle has landed." ---------------------------------------------------------- From owner-7tms_r@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!lhc.nlm.nih.gov!darwin.sura.net!maze.dpo.uab.edu!usenet From: Wayne Duck Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Re: Epitope Tagging Suggestions Wanted Date: Thu, 22 Feb 1996 13:50:03 -0600 Organization: University of Alabama at Birmingham Lines: 30 Message-ID: <312CC8EB.2533@phy164.physiology.uab.edu> References: NNTP-Posting-Host: neill.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.molbio.methds-reagnts:40806 bionet.molbio.proteins.7tms_r:634 A. John Watson wrote: > > I am planning to epitope tag one of my favorite receptors and am wondering > what systems have people found to be particularly reliable/unreliable with > respect to (a) choice of tag sequence and > (b) source of anti-tag antibody. > > I am aware of Brian Kobilka's Anal. Biochem. paper recommending the FLAG > tag and anti-FLAG antisera from Eastman Kodak. > > All responses appreciated. Will post tabulated responses for the dining > and dancing pleasure of all Response: We have found the HA tag to work very well for our purposes. The antibody is anti-HA 12CA5 clone. -- ---------------------------------------------------------- Wayne Duck Dr. Jimmy D. Neill's Lab Dept. of Physiology and Biophysics guest2@phybio.bhs.uab.edu http://neill.physiology.uab.edu http://neill.physiology.uab.edu/wayne http://neill.physiology.uab.edu/wayne/auball.htm "War Eagle" "Houston, Tranquility base here, The Eagle has landed." ---------------------------------------------------------- From owner-7tms_r@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!war.wyeth.com!hadcocj From: hadcocj@war.wyeth.com (John Hadcock) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 22 Feb 1996 11:21:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe 7tms_r hadcocj@war.wyeth.com From owner-7tms_r@net.bio.net Thu Feb 22 22:00:00 1996 Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!peer-news.britain.eu.net!newsfeed.ed.ac.uk!leeds.ac.uk!news From: bmbckh@biovax.leeds.ac.uk (Dr. Arthur L. Kruckeberg) Subject: Re: Epitope Tagging Suggestions Wanted Originator: -Not-Authenticated-[9535]@leeds.ac.uk Message-ID: <1996Feb23.170126.21876@leeds.ac.uk> NNTP-Posting-Host: bmbmac01.leeds.ac.uk Xdisclaimer: No attempt was made to authenticate the sender's name. Organization: Department of Biochemistry and Molecular Biology X-Posted-From: InterNews 1.0@gps.leeds.ac.uk. Date: Fri, 23 Feb 1996 17:01:26 +0000 (GMT) References: <312CC8EB.2533@phy164.physiology.uab.edu> Lines: 30 Xref: biosci bionet.molbio.methds-reagnts:40849 bionet.molbio.proteins.7tms_r:636 In article <312CC8EB.2533@phy164.physiology.uab.edu> Wayne Duck writes: > A. John Watson wrote: > > > > I am planning to epitope tag one of my favorite receptors and am wondering > > what systems have people found to be particularly reliable/unreliable with > > respect to (a) choice of tag sequence and > > (b) source of anti-tag antibody. > > > > I am aware of Brian Kobilka's Anal. Biochem. paper recommending the FLAG > > tag and anti-FLAG antisera from Eastman Kodak. > > > > All responses appreciated. Will post tabulated responses for the dining > > and dancing pleasure of all > > Response: Why not try the Strep-tag from Biometra. The tag is small and designed to bind to streptavidin. Good for both detection by Western and afinity purification. ------------------------------------------------------ Chris Hoyle Department of Biochemistry and Molecular Biology University of Leeds phone +44 0113 2333172 Leeds LS2 9JT FAX +44 0113 2333167 Great Britain e-mail bmbckh@biovax.leeds.ac.uk ------------------------------------------------------ From owner-7tms_r@net.bio.net Sat Feb 24 22:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!ra.nrl.navy.mil!news.math.psu.edu!chi-news.cic.net!newsfeed.internetmci.com!xmission!charnel.ecst.csuchico.edu!csusac!csus.edu!news.ucdavis.edu!rocky.ucdavis.edu!ez025409 From: zoom Newsgroups: bionet.molbio.proteins.7tms_r Subject: job opportunity Date: Sat, 24 Feb 1996 11:17:46 -0800 Organization: University of California, Davis Lines: 11 Message-ID: NNTP-Posting-Host: rocky.ucdavis.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: ez025409@rocky.ucdavis.edu I am a B.S. biochemistry graduate from U.C. Davis,and I am looking for a laboratory technician or research assistant postion in the field of molecular biology or a related field. I prefer to work either in Davis, or Sacramento. Does anyone know of any openings? I have four months experience in a temporary molecular biology position, with PCR, RNA and DNA extraction, hybridization, agarose gels, southern blotting, cell culture, and oligonucleotide labelling. Thank you, Sonya Sheth From owner-7tms_r@net.bio.net Sat Feb 24 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Re: Epitope Tagging Suggestions Wanted Followup-To: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Date: 25 Feb 1996 12:11:55 GMT Organization: University of Wuerzburg, Germany Lines: 35 Message-ID: <4gpjmb$dia@winx03.informatik.uni-wuerzburg.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.molbio.methds-reagnts:40914 bionet.molbio.proteins.7tms_r:639 Dr. Arthur L. Kruckeberg (bmbckh@biovax.leeds.ac.uk) wrote: [as a reply to a question which epitope tag to use?] > Why not try the Strep-tag from Biometra. The tag is small and designed > to bind to streptavidin. Good for both detection by Western and afinity > purification. As published recently by Arne Skerra, the original Strep-tag works only when attached at the C-terminus of a protein. He developed a modified Tag which works both at the C- and N-terminus. The reference is: T. G. M. Schmidt, J. Koepke, R. Frank, A. Skerra: Molecular interaction between the Strep tag affinity peptide and its cognate target streptavidin. J. Mol. Biol. 255, 753-766, 1996. Abstract available at http://www.hbuk.co.uk/jmb/articles/2555/2555-a08.htm Full article available (for subscribers only) at http://www.hbuk.co.uk/jmb/articles/2555/2555-a08.pdf --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun Feb 25 22:00:00 1996 Path: biosci!ISERVER.ICON.PALO-ALTO.MED.VA.GOV!bbh From: bbh@ISERVER.ICON.PALO-ALTO.MED.VA.GOV (Brian Hoffman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe second request Date: 26 Feb 1996 10:50:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Mon Feb 26 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!nnrp.info.ucla.edu!arle From: arle@biovax.leeds.ac.uk (Arle Kruckeberg) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Re: Epitope Tagging Suggestions Wanted Date: 27 Feb 1996 23:41:04 GMT Organization: Dept. of Biochemistry, Univ. of Leeds Lines: 33 Sender: -Not-Authenticated-[4360] Message-ID: <4h04qg$tdo@saba.info.ucla.edu> References: <4gpjmb$dia@winx03.informatik.uni-wuerzburg.de> NNTP-Posting-Host: 149.142.237.71 X-Posted-From: InterNews 1.0@saba.info.ucla.edu. Xdisclaimer: No attempt was made to authenticate the sender's name. Xref: biosci bionet.molbio.methds-reagnts:41040 bionet.molbio.proteins.7tms_r:641 In article <4gpjmb$dia@winx03.informatik.uni-wuerzburg.de> krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) writes: > Dr. Arthur L. Kruckeberg (bmbckh@biovax.leeds.ac.uk) wrote: > [as a reply to a question which epitope tag to use?] > > > Why not try the Strep-tag from Biometra. The tag is small and designed I didn't write this! It was Chris Hoyle, who hasn't reconfigured the news-reader to his own name... I have had good success with the phage T4 gene 10 epitope at the C-terminus of a membrane protein. The peptide is of neutral charge, so doesn't break von Heijne's Positive Inside rule (HA, FLAG and c-myc are negatively charged), it works away from the N-terminus even though it is from the N-terminus of the gene 10 protein, and for 2 proteins, the tagged version is 'fully' functional. The monoclonal doesn't light anything else up in a Saccharomyces cerevisiae crude membrane prep, on a Western. Haven't tried IP or IF yet, nor compared signal strength of multiple vs single tags. The monoclonal is available from Novagen, the epitope codons were introduced by overlap-extension PCR. Not associated with Novagen, though they kindly gave me a free sample to try on yeast extracts. Arle ************************************************* a.k.a. Dr. Arthur L. Kruckeberg Department of Biochemistry and Molecular Biology University of Leeds phone +44 +113 2333172 Leeds LS2 9JT FAX +44 +113 2333167 Great Britain arle@biovax.leeds.ac.uk ************************************************* From owner-7tms_r@net.bio.net Wed Feb 28 22:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!ra.nrl.navy.mil!news.math.psu.edu!chi-news.cic.net!newsxfer2.itd.umich.edu!gatech!willis.cis.uab.edu!maze.dpo.uab.edu!usenet From: Wayne Duck Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Re: Epitope Tagging Suggestions Wanted Date: Wed, 28 Feb 1996 20:26:07 -0600 Organization: University of Alabama at Birmingham Lines: 14 Message-ID: <31350EBF.4232@phy164.physiology.uab.edu> References: <4gpjmb$dia@winx03.informatik.uni-wuerzburg.de> <4h04qg$tdo@saba.info.ucla.edu> NNTP-Posting-Host: neill.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.molbio.methds-reagnts:41160 bionet.molbio.proteins.7tms_r:642 Arle Kruckeberg wrote: > The peptide is of neutral charge, so > doesn't break von Heijne's Positive Inside rule (HA, FLAG and c-myc are > negatively charged), Forgive me for my ignorance, but what is the von Heijne's Positive Inside rule? -- Wayne Duck mailto:wayne-duck@phy164.physiology.uab.edu mailto:guest2@phybio.bhs.uab.edu / http://neill.physiology.uab.edu Dept. of Physiology and Biophysics / University of Alabama at Birmingham "Houston, Tranquility base here, The Eagle has landed." From owner-7tms_r@net.bio.net Wed Feb 28 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech!newsfeed.internetmci.com!news.sprintlink.net!news.binc.net!news From: Jennifer Lyon Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Subject: Re: Epitope Tagging Suggestions Wanted Date: Thu, 29 Feb 1996 13:34:07 -0800 Organization: Berbee Information Networks Corporation Lines: 18 Message-ID: <31361BCF.421C@novagen.com> References: <4gpjmb$dia@winx03.informatik.uni-wuerzburg.de> <4h04qg$tdo@saba.info.ucla.edu> NNTP-Posting-Host: msn_3_3.binc.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.molbio.methds-reagnts:41186 bionet.molbio.proteins.7tms_r:643 ActArle Kruckeberg wrote: >... I have had good success with the phage T4 gene 10 epitope at the > C-terminus of a membrane protein. Actually, it is phage T7, not T4. Not that that really makes a difference to its function as an epitope tag. -Jenny (who IS 'associated' with Novagen.) -------------------------------------------------------------------------JennyAnn@ix.netcom.com Jenni10647@AOL.com "Sometimes the need to mess with their heads outweighs the millstone of humiliation." Fox Mulder "The X-Files" "What's the going rate on putting away an innocent man for the rest of his life, Doctor?" Teddy Hoffman "Murder One" ------------------------------------------------------------------------