From owner-7tms_r@net.bio.net Mon Mar 04 22:00:00 1996 Path: biosci!sbphrd.com!Jonathan_A_Lee From: Jonathan_A_Lee@sbphrd.com (Jonathan A Lee) Newsgroups: bionet.molbio.proteins.7tms_r Subject: PH.D. JOB OPENING Date: 5 Mar 1996 12:30:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <9603052327.AA5461@pho903.sbphrd.com> NNTP-Posting-Host: net.bio.net SIGNAL TRANSDUCTION EVENTS MEDIATED BY G-PROTEIN COUPLED RECEPTORS Ph. D. Investigator Department of Macromolecular Sciences SmithKline Beecham Pharmaceuticals POSITION: The investigator will join an ongoing effort to develop high throughput functional assays to characterize proprietary orphan GPCRs and develop therapeutic agents targeted to the interface between G-proteins and their receptor and effector molecules. REQUIREMENTS: Ph.D. and post-doctoral experience in either Cell Biology, Biochemistry, Pharmacology or a related field. Experience in a variety of experimental techniques used to monitor GPCR signal transduction (determination of cAMP, phospholipase activity, Ca levels, electrophysiology, reporter genes). Additional experience with the functional interaction of GPCRs with tyrosine kinase receptors and MAP kinases or structure-function relationships of GPCRs or G-proteins is desirable. Experience with tissue culture and molecular biology techniques required. THE DEPARTMENT: Macromolecular Sciences is a structurally oriented biochemistry group and uses a multidisciplinary approach to drug discovery. Departmental research areas include macromolecular X-ray crystallography, antibody and protein engineering, physical biochemistry, and GPCR structure-function studies. SmithKline is located in suburban Philadelphia, approximately 20 minutes from center city and life with the cows. FOR CONSIDERATION: Send curriculum vitae to: Jonathan A. Lee, Ph.D. SmithKline Beecham Pharmaceuticals TEL: 610-270-7588 Dept. Macromolecular Science FAX: 610-270-409 Mail Code UE 0447 Email: Jonathan_A_Lee@sbphrd.com 709 Swedeland Road King of Prussia, PA 19406 From owner-7tms_r@net.bio.net Tue Mar 05 22:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!news.mtu.edu!msunews!netnews.upenn.edu!NewsWatcher!user From: keller@email.chop.edu (Margaret A. Keller, Ph.D.) Newsgroups: bionet.molbio.proteins.7tms_r Subject: WWW Web Page for Molecular Biology Core Facility Date: Wed, 06 Mar 1996 15:55:13 -0500 Organization: Nucleic Acid/Protein Research Core Facility, The Children's Hospital of Philadelphia Lines: 6 Distribution: world Message-ID: References: <9603052327.AA5461@pho903.sbphrd.com> NNTP-Posting-Host: 159.14.43.40 The Nucleic Acid/Protein Research Core Facility of The Children's Hospital of Philadelphia is pleased to announce the posting of our Home Page. It describes many of the services we offer, including automated DNA sequencing and peptide synthesis, to name a few. Our address is http://www.med.upenn.edu/~phematol/napcore/ Feedback is welcome. From owner-7tms_r@net.bio.net Thu Mar 07 22:00:00 1996 Path: biosci!THALAMUS.WUSTL.EDU!brodbecr From: brodbecr@THALAMUS.WUSTL.EDU (Robbin Brodbeck) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Mystery Mutant Date: 8 Mar 1996 07:07:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603081503.JAA11843@thalamus.wustl.edu> NNTP-Posting-Host: net.bio.net Dear 7TMers: Roughly 6-8 weeks ago I came across a reference in which a mutant 7TM GPCR was expressed and assayed with respect to binding and second messenger production...by itself, not a novel occurance, but the specific mutation was intriging to me, and I need some help remembering what the reference was. The specific mutation was a tyr to phe mutation in TM5...residue #216 in the NK-1 receptor. If anyone could help me find this mentally-misplaced-mutation I would appreciate it Thanks in advance, Robb ----------------------- Robbin M. Brodbeck, Ph.D. brodbecr@thalamus.wustl.edu Washington University School of Medicine 660 S. Euclid ave. Dept. of Anatomy and Neurobiology Box 8108 St. Louis, MO. 63110 (314) 362-3468 Fax (314) 362-3446 From owner-7tms_r@net.bio.net Thu Mar 07 22:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!usenet.eel.ufl.edu!usenet.cis.ufl.edu!usenet.ufl.edu!usenet From: Stephen Nowicki Newsgroups: bionet.molbio.proteins.7tms_r Subject: Dopamine or alpha receptors on L cells Date: Fri, 08 Mar 1996 19:11:22 +0000 Organization: University of Florida Lines: 18 Message-ID: <3140865A.635F@qm.server.ufl.edu> References: <199603081503.JAA11843@thalamus.wustl.edu> Reply-To: jkhlab@qm.server.ufl.edu NNTP-Posting-Host: pharm187.med.ufl.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) Hello, I recall a discussion awhile back about endogenous receptors expressed on many of the cell lines that we artificially express receptors for study. However, I don't recall a discussion of whether mouse L cells, which have been used extensively to express the beta-Adrenergic receptor, contain subtypes of the Dopamine or alpha adrenergic receptors. I would like to know if there are either dopamine or alpha adrenergic receptor subtypes expressed normally on mouse L cells. Please post or feel free to email directly. I will summarize the responses for possible inclusion in the FAQ. Thank you, Stephen T. Nowicki University of Florida Department of Pharmacology email: jkhlab@qm.server.ufl.edu From owner-7tms_r@net.bio.net Sun Mar 10 22:00:00 1996 Path: biosci!YALE.EDU!henrik.dohlman From: henrik.dohlman@YALE.EDU ("Henrik Dohlman") Newsgroups: bionet.molbio.proteins.7tms_r Subject: G protein expression Date: 11 Mar 1996 15:03:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Subject: Time:5:57 PM OFFICE MEMO G protein expression Date:3/11/96 Can anyone direct me to a compilation of G protein alpha subunit expression patterns (e.g. by in situ hybridization), particularly in the brain? Many thanks. Henrik From owner-7tms_r@net.bio.net Sun Mar 10 22:00:00 1996 Path: biosci!aecom.yu.edu!angelett From: angelett@aecom.yu.edu (Ruth Angeletti) Newsgroups: bionet.molbio.proteins.7tms_r Subject: expression vectors Date: 11 Mar 1996 12:04:58 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603111947.OAA13176@medusa.bioc.aecom.yu.edu> NNTP-Posting-Host: net.bio.net Which vectors have been found most effective for expression of GPCRs in mammalian cells? Thanks in advance, Ruth Ruth Hogue Angeletti Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx NY 10461 tel:718-430-3475 fax:718-430-8939 angelett@aecom.yu.edu From owner-7tms_r@net.bio.net Sun Mar 10 22:00:00 1996 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: water systems Date: 11 Mar 1996 05:12:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <960311.080928.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net I am about to invest in an ultrapure water system, and am having some difficulty identifying "real" differences among the various vendors, all of whom claim to have the best system ever. In lieu of a "consumer reports of scientific instrumentation", I am seeking the advice (pro or con) of the networked multitudes. For example, a number of years ago I was in a lab where a Barnstead RGW5 kept blowing out final stage filters ($200 each!), but I know they have completely redesigned the system. Anyone know how well they work? Any good/bad with Milli-Q? Which one? IF YOU HAVE _ANY_ SUGGESTIONS (PRO OR CON) ABOUT ULTRAPURE WATER SYSTEMS, PLEASE SEND ME A POST. IF THERE'S INTEREST, I'LL POST THE AGGREGATE RESPONSES TO THE GROUP(S). Thank you for your help. PS - If anyone knows of a "scientific instruments consumer reports" home page, please let me know. Alternatively, does anyone out there have the time/interest/computer resources to initiate such a site? | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-1553 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O | From owner-7tms_r@net.bio.net Sun Mar 10 22:00:00 1996 Path: biosci!CORR.COM!pamela_conley From: pamela_conley@CORR.COM ("PAMELA CONLEY") Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 11 Mar 1996 18:38:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe 7tms_r From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!csn!nntp-xfer-2.csn.net!tali.UCHSC.edu!chris From: Firstname.Lastname@UCHSC.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: expression vectors Date: 12 Mar 1996 16:38:09 GMT Organization: University of Colorado Health Sciences Center Lines: 8 Message-ID: <4i499h$1ve@tali.UCHSC.edu> References: <199603111947.OAA13176@medusa.bioc.aecom.yu.edu> NNTP-Posting-Host: peptide.uchsc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) To: Ruth,Angeletti For a comprehensive review of expression of membrane proteins, see: R. Grisshammer & C.G. Tate, Overexpression of integral membrane proteins for structural studies Quart. Rev. Biophys 28: 315-442, 1995 John Stewart From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!csn!nntp-xfer-2.csn.net!tali.UCHSC.edu!chris From: Firstname.Lastname@UCHSC.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: expression vectors Date: 12 Mar 1996 16:39:20 GMT Organization: University of Colorado Health Sciences Center Lines: 8 Message-ID: <4i49bo$1ve@tali.UCHSC.edu> References: <199603111947.OAA13176@medusa.bioc.aecom.yu.edu> NNTP-Posting-Host: peptide.uchsc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) To: Ruth,Angeletti, For a comprehensive review of expression of membrane proteins, see: R. Grisshammer & C.G. Tate, Overexpression of integral membrane proteins for structural studies Quart. Rev. Biophys 28: 315-442, 1995 John Stewart From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!ccmail.med.nyu.edu!ERIC.SIMON From: ERIC.SIMON@ccmail.med.nyu.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 12 Mar 1996 09:53:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I292Q13WAA8WX8KC@MCRCR6.MED.NYU.EDU> NNTP-Posting-Host: net.bio.net This is my third message. Please unsubscribe me from 7tms_r@net.bio.net discussion group. Thank you. From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: How to unsubscribe Date: Tue, 12 Mar 1996 16:03:01 -0500 Organization: University of Michigan Lines: 165 Message-ID: <3145E685.2D79@umich.edu> References: <01I292Q13WAA8WX8KC@MCRCR6.MED.NYU.EDU> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) To: ERIC.SIMON@ccmail.med.nyu.edu ERIC.SIMON@ccmail.med.nyu.edu wrote: > > This is my third message. Please unsubscribe me from > 7tms_r@net.bio.net discussion group. Thank you. Eric, You need to unsubscribe yourself. Here's some info about the 7tms_r newsgroup and about newsgroups in general. ------------------------------------------------------------------ The short answer re subscribing and unsubscribing in North America is to send a message with one line to biosci-server@net.bio.net To unsubscribe it should come from your old address and say: unsubscribe 7tms_r To subscribe it should come from your new address and say: subscribe 7tms_r More detailed information follows re BIOSCI newsgroup subscriptions. Cheers, Rick _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 E-mail Subscription Requests and other Information -------------------------------------------------- For users in the Americas and Pacific Rim countries, e-mail subscription and cancellation requests are handled automatically by an e-mail server, although personal assistance is also available via the biosci-help@net.bio.net address. Once your e-mail address is added to a subscription list, mail will be sent to your address automatically each time someone posts a message. This will continue until you remove your address from the subscription list. You should first send the lists command to the address biosci-server@net.bio.net to obtain a listing of all current BIOSCI mailing lists. You may leave your message Subject: line blank in any message that is sent to the server (text on the Subject: line is *not* processed). Please include all commands only in the body of your mail message. Please BE SURE to substitute the appropriate mailing list name obtained FROM THE "lists" COMMAND OUTPUT for "listname" in the subscribe or unsubscribe commands shown below. Some list names used by the server are abbreviated compared to their full spelling in the BIOSCI information sheet. After obtaining the names of the mailing lists using the "lists" command, use the subscribe listname or unsubscribe listname commands to add or delete your address from the mailing lists. Please substitute "listname" above with the appropriate name of the list that you want to join/cancel. !!! PLEASE BE SURE to send all subscribe or unsubscribe commands only to !!! the biosci-server@net.bio.net address and not to the newsgroup posting !!! addresses where they will bother all of the readers! Your e-mail address is obtained AUTOMATICALLY from the header of your mail message and should NOT be included on the subscribe/unsubscribe command line unless you need to sign up at a different address than the one you are currently using. Such requests must be handled manually by us and create extra work. (Also you would be surprised at how many people list their e-mail addresses incorrectly, so please let our server obtain this information automatically from your mail header.) Multiple commands may be placed on separate lines in the same mail message, i.e., you can include all of your subscribe and/or unsubscribe requests in one mail message as long as each is on a separate line. If you put multiple commands in your mail message, please put an end command as the last in your list of commands. This helps avoid sending to the server any signatures that might be automatically included in your mail messages. Here is a sample subscription message. The address public@state-u.edu would be automatically added to the bionews and grasses mailing lists as the result of this message. To: biosci-server@net.bio.net From: J.Q. Public Subject: subscribe bionews subscribe grasses end If you are already a BIOSCI e-mail subscriber and need to determine which mailing lists you are on currently, send the command which to biosci-server@net.bio.net if you are signed up under the address which will appear on the From: line of your mail message. If your address has changed, you can put a search string after the which command, e.g., if you are certain than your user name has always been jqpublic but do not remember the computer hostname, send the command which jqpublic Other command details are included in the mail server help file from biosci-server@net.bio.net. To get the server help file (in computerese 8-), send in the help command to the server address. E-mail Address Changes ---------------------- If you have subscribed to a newsgroup and are now leaving an institution or changing your e-mail address, it is IMPERATIVE that you cancel your subscription! Non-existent addresses or overflowing mailboxes cause computer mail programs to send back "daemon" messages which might bother everybody on the newsgroup. We will immediately remove any address causing such a problem, but would prefer it if you would notify us in advance as a courtesy to the rest of the user community. Interruption of E-mail Service ------------------------------ It is our policy to remove any address from our mailing lists which becomes inaccessible and causes mail to bounce back to the sender. This might happen to you if your local computer or network fails for a significant period of time. If you notice that you are no longer receiving BIOSCI postings, it may be because your address was removed for the above reason. It will be necessary for you to contact biosci-help@net.bio.net and resubscribe. Please see the BIOSCI FAQ, mentioned at the beginning of this document, for more details on how BIOSCI handles addresses which reject mail. ==================== end of BIOSCI info ==================== _________________________________________________________ Rick Neubig RNeubig@umich.edu Department of Pharmacology University of Michigan Phone (313) 763-3650 FAX (313) 763-4450 http://www-personal.umich.edu/~rneubig/ From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!war.wyeth.com!hadcocj From: hadcocj@war.wyeth.com (John Hadcock) Newsgroups: bionet.molbio.proteins.7tms_r Subject: expression vectors -Reply Date: 12 Mar 1996 03:33:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net We have used pRC/CMV and pcDNA3 (both Invitrogen) with very good success for stable transfections of somatostatin, adenosine and adrenergic receptors (up to 50 pmol/mg protein). John Hadcock hadcocj@war.wyeth.com From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 12 Mar 1996 02:00:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 149 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603121000.CAA00995@net.bio.net> NNTP-Posting-Host: net.bio.net I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We have three sponsors to date with a couple more pending. The process is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we hope to have new and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Statistics are for the four week period from 22 Jan. - 18 Feb. 1996 and usage continues to grow. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5000 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 600 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. ---------------------------------------------------------------------- From owner-7tms_r@net.bio.net Mon Mar 11 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!csn!nntp-xfer-2.csn.net!tali.UCHSC.edu!chris From: Firstname.Lastname@UCHSC.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: expression vectors Date: 12 Mar 1996 16:41:42 GMT Organization: University of Colorado Health Sciences Center Lines: 8 Message-ID: <4i49g6$1ve@tali.UCHSC.edu> References: <199603111947.OAA13176@medusa.bioc.aecom.yu.edu> NNTP-Posting-Host: peptide.uchsc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) To: angelett@aecom.yu.edu For a comprehensive review of expression of membrane proteins, see: R. Grisshammer & C.G. Tate, Overexpression of integral membrane proteins for structural studies Quart. Rev. Biophys 28: 315-442, 1995 John Stewart From owner-7tms_r@net.bio.net Tue Mar 12 22:00:00 1996 Path: biosci!CHET.MEDC.UMN.EDU!germana From: germana@CHET.MEDC.UMN.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCRs listing of meetings ? Date: 13 Mar 1996 08:40:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <9603131634.AA17320@quinn.medc.umn.edu> NNTP-Posting-Host: net.bio.net I am looking for information regarding upcoming meetings on GPCRs in the USA or Canada. Is there a web site with an updated list of meetings ? Cheers Germana Paterlini Dept. of Medicinal Chemistry University of Minnesota Minneapolis, MN 55455 germana@quinn.medc.umn.edu From owner-7tms_r@net.bio.net Tue Mar 12 22:00:00 1996 Path: biosci!cict.fr!bourbon From: bourbon@cict.fr (Henri Bourbon) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 13 Mar 1996 09:22:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603131719.SAA14232@oceane.cict.fr> NNTP-Posting-Host: net.bio.net unsubscribe 7tms_r ---------------------------------------------------------------------------- ------------------------------------ Henri-Marc G. Bourbon Centre de Biologie du D=E9veloppement 118, route de Narbonne 31062 Toulouse - France Tel : (33) 61.55.82.88 =46ax : (33) 61.55.65.07 Email : bourbon@cict.fr ---------------------------------------------------------------------------- ------------------------------------ From owner-7tms_r@net.bio.net Tue Mar 12 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.millennianet.com!tns.sdsu.edu!newshub.sdsu.edu!usenet From: Wanda Judge Newsgroups: bionet.molbio.proteins.7tms_r Subject: Test Date: 13 Mar 1996 19:03:17 GMT Organization: San Diego State University Lines: 8 Message-ID: <4i765l$r55@hole.sdsu.edu> NNTP-Posting-Host: 130.191.125.11 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: rickel@rohan.sdsu.edu,judge@mail.sdsu.edu Further test I have no idea even what this topic refers to except that it is about proteins of some type. Hopefully I would have some idea of what I want say when posting to a newsgroup. And thats all I have to say about this first attempt! From owner-7tms_r@net.bio.net Tue Mar 12 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.millennianet.com!tns.sdsu.edu!newshub.sdsu.edu!usenet From: Wanda Judge Newsgroups: bionet.molbio.proteins.7tms_r Subject: test Date: 13 Mar 1996 18:42:22 GMT Organization: San Diego State University Lines: 2 Message-ID: <4i74ue$r55@hole.sdsu.edu> NNTP-Posting-Host: 130.191.125.11 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) This is a test. From owner-7tms_r@net.bio.net Tue Mar 12 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.millennianet.com!tns.sdsu.edu!newshub.sdsu.edu!usenet From: Wanda Judge Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: test message Date: 13 Mar 1996 18:47:36 GMT Organization: San Diego State University Lines: 2 Message-ID: <4i7588$r55@hole.sdsu.edu> References: <4i74ue$r55@hole.sdsu.edu> NNTP-Posting-Host: 130.191.125.11 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Further tests From owner-7tms_r@net.bio.net Wed Mar 13 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!news.uit.no!atf1!edvard From: edvard@atf1.fm.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: ANNOUNCE: Mutant database GRAP Date: 14 Mar 1996 11:35:12 GMT Organization: University of Tromsoe, Norway Lines: 75 Distribution: world Message-ID: <4i909g$3nv@news.uit.no> Reply-To: edvard@atf1.fm.uit.no NNTP-Posting-Host: atf1.imb.fm.uit.no We are pleased to announce that our mutant database, GRAP is available to the 7tms community. GRAP is an acronym for G-protein coupled Receptor, family A, Point mutation database. The database is available on A paper on the database has been accepted for publication: Kristiansen, K., Dahl, S. G., Edvardsen, Ø.: A database of mutants and effects of site-directed mutagenesis experiments on G-protein coupled receptors. PROTEINS: Struct., Funct. and Genet. Data for single and multiple point mutants of members of family A of GPCRs, described in published journal articles in English language have been collected and is available for searching. The database contains data for multiple point mutants where a low number of amino acids (up to 12) has been substituted, including some chimeric receptors where small segments were exchanged without any deletions or insertions of amino acids. The database contains data on the substitution, domain, receptor class, literature reference, and quantitative and qualitative data related to agonist binding, antagonist binding and signal transduction. Various types of user-defined queries can be performed: 1. Substitution oriented queries, including wild-card queries on receptor types, domains and substitutions; e.g. to find all substitutions where Asn in TMH7 has been substituted by Thr, Ala or Phe in serotoninergic and adrenergic receptors. 2. Ligand oriented queries may be performed by searching for full or partial ligand names. 3. Bibliographic oriented queries may be performed by searching for e.g. a full or partial author name. 4. Sequence oriented 'queries' may be performed by accessing the mutant data from a multiple sequence alignment where all substituted amino acids have links to the data. Whatever entry point you choose, you have access to all data via links between the various types of information. Database statistics: Total number of substitutions: 3551 Total number of positions: 1732 Total number of mutants: 2136 Number of single point mutants: 1597 Number of multiple point mutants: 539 Number of subtypes with registered substitutions: 94 Number of species with registered substitutions: 8 Number of papers referenced: 332 Research assisted by this service should cite the paper which will appear in PROTEINS. The exact literature reference will be available on the URL above as soon as it is available to us. Contact information: * Software and technical issues: Øyvind Edvardsen, edvard@fagmed.uit.no * Data-related issues: Kurt Kristiansen, kurtk@fagmed.uit.no -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Thu Mar 14 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!nntp.coast.net!fu-berlin.de!zrz.TU-Berlin.DE!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Test Followup-To: bionet.general Date: 15 Mar 1996 11:13:40 GMT Organization: University of Wuerzburg, Germany Lines: 21 Message-ID: <4ibjd4$jr@winx03.informatik.uni-wuerzburg.de> References: <4i765l$r55@hole.sdsu.edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Wanda Judge (judge@rohan.sdsu.edu) wrote: [three test messages in a row] This group is not about testing, but about G protein-coupled receptors. If you want to check out whether your news software works properly, send test messages either to misc.test or alt.test. You will even get a reply from several servers around the world which monitor these newsgroups. I have suggested once to create a bionet.test group to account for the special mechanisms of posting that are available through bionet. Maybe it's time to revitalize this idea again. Followups to bionet.general. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Mar 14 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!howland.reston.ans.net!EU.net!Belgium.EU.net!news From: "MertensR@JRF.be" Newsgroups: bionet.molbio.proteins.7tms_r Subject: (no subject) Date: 15 Mar 1996 15:01:02 GMT Organization: Janssen Research Foundation Belgium Lines: 8 Message-ID: <4ic0ne$lh5@news.Belgium.EU.net> NNTP-Posting-Host: pc4.cim.jrf.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi, I was wondering if someone knows something about co-expression of G proteins and their receptors in Escherichia coli? Or is it just better to perform reconstitution experiments with phospholipid vesicles? From owner-7tms_r@net.bio.net Thu Mar 14 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!swrinde!howland.reston.ans.net!EU.net!Belgium.EU.net!news From: "MertensR@JRF.be" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: test Date: 15 Mar 1996 14:58:12 GMT Organization: Janssen Research Foundation Belgium Lines: 6 Message-ID: <4ic0i4$lh5@news.Belgium.EU.net> References: <4i74ue$r55@hole.sdsu.edu> NNTP-Posting-Host: pc4.cim.jrf.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: judge@rohan.sdsu.edu Congratulations with your test, how did you get in? I'm not a computer freak and I'm trying desperately to get into the group. email:jvanhauw@janbe.jnj.com From owner-7tms_r@net.bio.net Thu Mar 14 22:00:00 1996 Path: biosci!MSMAIL.BMS.COM!Wiener#m#_Harvey_L#d#.Wallingford_Mail_Server From: Wiener#m#_Harvey_L#d#.Wallingford_Mail_Server@MSMAIL.BMS.COM ("Wiener, Harvey L.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Beckman Biomek 2000 Date: 15 Mar 1996 12:41:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net My laboratory recently purchased a Beckman Biomek 2000 liquid handling station for radioligand binding assays. We find that the precision for binding assays with a cloned human receptor expressed in high density is terrible. Are there any members on this group who use the Biomek 2000? If so, what advice do you have? You can E-mail me directly and I will compile answers and post them if there is interest in doing so. Thanks for your help. Harvey L. Wiener CNS Drug Discovery Bristol-Myers Squibb Co. Wallingford, CT 06492 (203) 284-6919 Wiener#m#_Harvey_L#d#.Wallingford_Mail_Server@msmail.bms.com From owner-7tms_r@net.bio.net Fri Mar 15 22:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!nntp.coast.net!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: (no subject) Date: 16 Mar 1996 15:28:43 GMT Organization: University of Wuerzburg, Germany Lines: 20 Message-ID: <4iemnb$2rp@winx03.informatik.uni-wuerzburg.de> References: <4ic0ne$lh5@news.Belgium.EU.net> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] MertensR@JRF.be (MertensR@JRF.be) wrote: > I was wondering if someone knows something about co-expression of G > proteins and their receptors in Escherichia coli? Or is it just better to > perform reconstitution experiments with phospholipid vesicles? I have no idea whether anybody ever has expressed alpha subunits of G proteins together with receptors. AFAIK beta-gamma subunits of G proteins cannot be expressed in E. coli successfully since they need posttranslational modifications for proper function. To make things worse, G-protein-coupled receptors are poorly expressed in E.coli. OTOH, reconstitution in phospholipid vesicles may work fine when you can purify the receptor. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun Mar 17 22:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!Belgium.EU.net!news From: "MertensR@JRF.be" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: (no subject) Date: 18 Mar 1996 15:45:31 GMT Organization: Janssen Research Foundation Belgium Lines: 14 Message-ID: <4ik0er$c7a@news.Belgium.EU.net> References: <4ic0ne$lh5@news.Belgium.EU.net> <4iemnb$2rp@winx03.informatik.uni-wuerzburg.de> NNTP-Posting-Host: pc4.cim.jrf.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: krasel@wpxx02.toxi.uni-wuerzburg.de Thank you for responding to my request. As far as I now (for the moment), it is possible to express G protein coupled receptors (because I've expressed now a dopamine and a serotonin receptor in E. coli). It also is possible to express G proteins (e.g. Gilman et al), but those two expressing in the same time was, and still is a great question mark to me. Anyway, should you have any more suggestions, questions or remarks, I'd be very glad to discuss them. (My aim is to reconstitute the whole transduction pathway in E. coli, or if not possible to reconstitute it in vitro). By the way, my name is Jurgen Vanhauwe and I'm working at the university of Gent in the laboratories of the Janssen Research Foundation. My email adress is:jvanhauw@janbe.jnj.com From owner-7tms_r@net.bio.net Sun Mar 17 22:00:00 1996 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post doc position Date: 18 Mar 1996 14:29:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603182226.WAA28272@hobby.embl-heidelberg.de> NNTP-Posting-Host: net.bio.net We are looking for a post-doc to help us improve the GPCRDB database. The candidate should have - experience programming Fortran and/or C - some system management experience - good knowledge of a scripting language (e.g. Perl, python, etc.) - Some basic biological and/or chemical knowledge - Interest in biological processes - Adequate knowledge of the English language (native English not needed, but the candidate should be able to communicate with us...) Additionally we expect the candidate - to have a flexible mind so that he/she can quickly respond to new questions - to work user-oriented - to go to meetings - to be open to new knowledge, i.e., to never stop learning - to participate in the other work the group has to do (not much, but it has to happen) We offer a competitive salary, a very good working environment (all software and hardware to do the job right is available; a modern workstation is already waiting to be used). We are located in the biocomputing unit of the EMBL in Heidelberg, Germany. The language most used in the lab is English. If you know of any candidate, please pass this message on to him/her. Potential candidates can look at: http://swift.embl-heidelberg.de/ to get an impression of kinds of work we do in biocomputing at the EMBL. Please mail to Gert Vriend ("Vriend@EMBL-Heidelberg.DE") for further information. From owner-7tms_r@net.bio.net Mon Mar 18 22:00:00 1996 Path: biosci!AUSTIN.UNIMELB.EDU.AU!tracy From: tracy@AUSTIN.UNIMELB.EDU.AU (Tracy Nero) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Preliminary conference announcement RACI/Melbourne/Dec. 1996 Date: 19 Mar 1996 19:41:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603200323.NAA26799@tango.austin.unimelb.edu.au> NNTP-Posting-Host: net.bio.net Please excuse any cross posts. ROYAL AUSTRALIAN CHEMICAL INSTITUTE MEDICINAL & AGRICULTURAL DIVISION 13TH NATIONAL CONFERENCE "UP AND COMING RESEARCH IN AUSTRALIA" 8-11 DECEMBER, 1996, Monash University, Clayton 3168, Victoria, Australia The conference will cover all aspects of medicinal and agricultural chemistry. Papers are invited for both lecture and poster sessions. The meeting will be held in conjunction with the ASCEPT pharmacology conference and some joint sessions will be organized. The conference will focus on students and new researchers, emerging research areas in medicinal and agricultural chemistry, and the interaction of medicinal chemists and pharmacologists. There will also be a symposium on research funding and the research/industry interface. Preliminary session topics include: o agrochemistry o toxins o anti-infectives o enzyme inhibitors o automated methods of synthesis and screening o glycobiology and glycochemistry o DNA and drugs o QSAR methods o medicinal chemistry teaching. Registration forms and details will be published later to this list and on our Web page. Enquiries to: Dr. Margaret Wong, Department of Applied Chemistry, Swinburne University of Technology, Hawthorn 3122 Victoria Ph: (03)9214-8542 Fax: (03)9819-0834 email: marg@chem1.chem.swin.edu.au or me. Cheers, Tracy Tracy Nero, PhD Clinical Pharmacology and Therapeutics Unit, Dept. of Medicine, The University of Melbourne, Austin Campus, Austin and Repatriation Medical Centre, Heidelberg, 3084, Victoria, Australia tracy@austin.unimelb.edu.au Ph. 61 3 94965052 Fax 61 3 94593510 From owner-7tms_r@net.bio.net Mon Mar 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r,sci.research.postdoc Subject: POSTDOCTORAL POSITION AVAILABLE Date: 19 Mar 1996 15:52:08 -0800 Organization: Oregon Health Sciences University Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <4i4bkv$kve@nadine.teleport.com> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.molbio.proteins.7tms_r:676 sci.research.postdoc:4304 I have a postdoctoral position available beginning April 1, using molecular genetic and pharmacological approaches to the study of the dopamine system. Please check out my web page and email a resume if you are interested. Kim A. Neve, Ph.D. Associate Research Career Scientist http://www.teleport.com/~nevek From owner-7tms_r@net.bio.net Mon Mar 18 22:00:00 1996 Path: biosci!RUBENS.ITS.UNIMELB.EDU.AU!pms From: pms@RUBENS.ITS.UNIMELB.EDU.AU (Patrick Sexton) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 19 Mar 1996 14:26:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I2K1TOMV9E8WWQ95@rubens.its.unimelb.edu.au> NNTP-Posting-Host: net.bio.net subscribe 7tms_r@net.bio.net From owner-7tms_r@net.bio.net Mon Mar 18 22:00:00 1996 Path: biosci!OCELOT.RUTGERS.EDU!PDU From: PDU@OCELOT.RUTGERS.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: substructure search code? Date: 19 Mar 1996 05:47:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I2IM01B4Q8AKU08B@mbcl.rutgers.edu> NNTP-Posting-Host: net.bio.net Hi, everyone: Is there substructure searching code available? I need to identify a fragment with atoms and conectivity defined in a molecule, which also has atoms and connectivity known. Suggestions about the source code or algorithm for this purpose are greatly appreciated. Ping Du Synaptic Pharmaceutical Corp. From owner-7tms_r@net.bio.net Mon Mar 18 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!news.uit.no!news From: Kurt Kristiansen Newsgroups: bionet.molbio.proteins.7tms_r Subject: Constitutively active mutants in GRAP Date: 19 Mar 1996 10:56:12 GMT Organization: University of Tromsoe, Norway Lines: 19 Message-ID: <4im3sc$71b@news.uit.no> NNTP-Posting-Host: atf1.imb.fm.uit.no Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.3 sun4m) X-URL: file:/u4/kurtkr/sdmdb/constitu.txt We have created a list of the 65 constitutively active mutants that have been registered in GRAP. By clicking on a particular entry in this list, information for the mutant is displayed. The list of constitutively active mutants is available on GRAP is an acronym for G-protein coupled Receptor, family A, Point mutation database. The database is available on Contact information: * Software and technical issues: Oyvind Edvardsen, edvard@fagmed.uit.no * Data-related issues: Kurt Kristiansen, kurtk@fagmed.uit.no From owner-7tms_r@net.bio.net Tue Mar 19 22:00:00 1996 Path: biosci!MC.DUKE.EDU!koch0002 From: koch0002@MC.DUKE.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdocs Wanted Date: 20 Mar 1996 04:01:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I2JWT737JM0099AR@mc.duke.edu> NNTP-Posting-Host: net.bio.net I have a postdoctoral position available in my lab to study the role of adrenergic signal transduction in cardiac development. Studies involve knockout and transgenic mice and involve both receptors and receptor kinases. Other projects available in the lab include adrenergic-based gene transfer to adult cardio-pulmonary tissues. Interested parties may contact me via email. The position will be available approximately June 1, 1996. Walter J. Koch, Ph.D. Assistant Professor of Surgery Duke University Medical Center KOCH0002@mc.duke.edu From owner-7tms_r@net.bio.net Tue Mar 19 22:00:00 1996 Path: biosci!MOLDEV.COM!jack_owicki From: jack_owicki@MOLDEV.COM (Jack Owicki) Newsgroups: bionet.molbio.proteins.7tms_r Subject: <$70,000,000,000 per mole for chemokines??? Date: 20 Mar 1996 17:17:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net We would like to do some work with a variety of chemokines using our microphysiometry technology for functional cellular assays. But the chemokines are awfully expensive when purchased from a standard commercial source: typically $175 per 20 =B5g, which works out to around $7x10^10/mole= . We'd most likely be interested in ~10 nmole or ~100 =B5g quantities of a variety of chemokines. Can anyone suggest a more inexpensive source or a pointer to information on producing them yourself by recombinant means? Thanks much. Jack Owicki ------------------------------------------------------------ John C. Owicki, Ph.D. Associate Technical Director Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 E-mail: jack_owicki@moldev.com Phone: (408) 747-3514; Fax: (408) 747-3601 From owner-7tms_r@net.bio.net Wed Mar 20 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: GPCR affinity Date: Thu, 21 Mar 1996 18:15:51 -0500 Organization: University of Michigan Lines: 72 Message-ID: <3151E327.6D29@umich.edu> References: <31512BB1.48EA@biokemi.su.se> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 2.0 (Win95; I) My thoughts on this would include two factors. 1) There could be a = different potency order of affinity with and without G protein and 2) = functional assays will include significant contributions from drug = efficacy. Clearly the affinities of full agonists would be expected to be = distorted more than those of antagonists or partial agonists in = bacteria. A full agonist can have its affinity increased by at least 2 = or perhaps 3 orders of magnitude if the G protein is present. = Also, in a functional assay, there can be a large receptor reserve so = that a drug can stimulate responeses at a low receptor occupancy giving = what looks like a very high affinity if you simply take the EC50 as the = measure. The beta adrenergic receptor (see early work by E. Ross and = others and more recent work by Richard Clark) can show large Kd/EC50 = ratios for strong full agonists. Finally, an intriguing paper from Steve Liggett's lab suggests different = specificity for alpha2 receptors depending on the G protein involved Eason, M.G., Jacinto, M.C., and Liggett, S.B. Contribution of ligand = structure to activation of a2-adrenergic receptor subtype coupling to = Gs. Molecular Pharmacology 45:696-702, 1994. = Rick Hans Kiefer wrote: > = > Dear netters, > = > I am sure you all know, but I don=B4t: Is the selectivity of a GPCR > towards different ligands the same in the absence and presence of a > G-protein? I know the affinity is not, but is that a general > phenomenon or does it depend on the ligand? > I am asking, because I am testing various ligands on a GPCR expressed > in E. coli (no G-protein), and the results seem to be different from > what is known about second messenger response of the same GPCR in SF9 > cells. > Any literature hints? > Thanks, > = > Hans > N.B: What is the typical ratio for affinty + vs. - G-protein? -- = _________________________________________________________ Rick Neubig RNeubig@umich.edu Department of Pharmacology University of Michigan = Phone (313) 763-3650 FAX (313) 763-4450 http://www-personal.umich.edu/~rneubig/ From owner-7tms_r@net.bio.net Wed Mar 20 22:00:00 1996 Path: biosci!MOLDEV.COM!jack_owicki From: jack_owicki@MOLDEV.COM (Jack Owicki) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Rebroadcast, $$$$$ per mole for chemokines Date: 21 Mar 1996 08:59:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net When I received a copy of my message "<$70,000,000,000 per mole for chemokines???" over the newsgroup, it was garbled and truncated about halfway through. Don't know why; it's fine in my "out" mail box. But since it's likely that everyone received it messed up, here it is again.... We would like to do some work with a variety of chemokines using our microphysiometry technology for functional cellular assays. But the chemokines are awfully expensive when purchased from a standard commercial source: typically $175 per 20 ug, which works out to around $7x10^10/mole. We'd most likely be interested in ~10 nmole or ~100 ug quantities of a variety of chemokines. Can anyone suggest a more inexpensive source or a pointer to information on producing them yourself by recombinant means? Thanks much. Jack Owicki ------------------------------------------------------------ John C. Owicki, Ph.D. Associate Technical Director Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 E-mail: jack_owicki@moldev.com Phone: (408) 747-3514; Fax: (408) 747-3601 From owner-7tms_r@net.bio.net Wed Mar 20 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news.rediris.es!scsing.switch.ch!swsbe6.switch.ch!surfnet.nl!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.datakom.su.se!usenet From: Hans Kiefer Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCR affinity Date: Thu, 21 Mar 1996 11:13:05 +0100 Organization: Stockholm University Lines: 22 Message-ID: <31512BB1.48EA@biokemi.su.se> NNTP-Posting-Host: 130.237.179.57 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Dear netters, I am sure you all know, but I don=B4t: Is the selectivity of a GPCR = towards different ligands the same in the absence and presence of a = G-protein? I know the affinity is not, but is that a general = phenomenon or does it depend on the ligand? I am asking, because I am testing various ligands on a GPCR expressed = in E. coli (no G-protein), and the results seem to be different from = what is known about second messenger response of the same GPCR in SF9 = cells. Any literature hints? Thanks, Hans = N.B: What is the typical ratio for affinty + vs. - G-protein? From owner-7tms_r@net.bio.net Thu Mar 21 22:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!qiclab.scn.rain.com!psgrain!nntp.teleport.com!usenet From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r Subject: silent mutations Date: Fri, 22 Mar 1996 18:44:14 GMT Organization: Oregon Health Sciences University Lines: 10 Message-ID: <4iuse3$a90@nadine.teleport.com> NNTP-Posting-Host: ip-pdx04-46.teleport.com X-Newsreader: Forte Free Agent 1.0.82 I seem to recall that somebody once posted a message to this newsgroup about a web site or free program that can be used to identify potential silent mutations in a given sequence. Does anybody have information about such a site? Thanks Kim Neve Kim A. Neve, Ph.D. Associate Research Career Scientist http://www.teleport.com/~nevek From owner-7tms_r@net.bio.net Sat Mar 23 22:00:00 1996 Path: biosci!MIRIS.MED.YALE.EDU!mike From: mike@MIRIS.MED.YALE.EDU (Michael Singer) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 24 Mar 1996 08:28:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <9603241629.AA06360@miris.med.yale.edu> NNTP-Posting-Host: net.bio.net The Molecular Evolution Group at Penn State [M. Nei, A. Hughes, et al] produces a fine program called MEGA which will calculate numbers of silent mutations. The disk (as far as I know PC version only) is free; documentation is $15. Call or e-mail them for an order form. Michael S. Singer Section of Neurobiology Yale University School of Medicine From owner-7tms_r@net.bio.net Mon Mar 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!newsserver.jvnc.net!netnews.sbphrd.com!news From: Katherine_L_Widdowson Newsgroups: bionet.molbio.proteins.7tms_r Subject: animals with two IL-8 receptors Date: 26 Mar 1996 21:46:19 GMT Organization: SmithKline Beecham Lines: 10 Message-ID: <4j9ojb$7gs@phunn1.sbphrd.com> NNTP-Posting-Host: ued886.um.us.sbphrd.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) To: Katherine_L_Widdowson@sbphrd.com Is anybody aware of animals other than Human and Rabbit which contain two IL-8 receptors. -- The opinions expressed in this communication are my own, and do not necessarily reflect those of my employer. From owner-7tms_r@net.bio.net Thu Mar 28 22:00:00 1996 Path: biosci!uni-duesseldorf.de!schuessl From: schuessl@uni-duesseldorf.de (Peter Schuessler) Newsgroups: bionet.molbio.proteins.7tms_r Subject: ras and GAP expression Date: 29 Mar 1996 08:35:58 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603291635.IAA22078@net.bio.net> NNTP-Posting-Host: net.bio.net 7TMers, Has anybody information (e.g. a review) about a stage, tissue, or developmental expression profile of ras and GAP?? I'am interested to know whether the ras and GAP amount (!!) (mRNA or protein) is, for example, higher in a mitotic active tissue than in an inactive. Thanks, --------------------------------------------- | Peter Schuessler | | Dep.: Genetic Parasitology | | Institute for Genetic | | Heinrich-Heine-University Duesseldorf | | Universitaetsstr.1 | | D-40225 Duesseldorf | | Germany | | | | Tel: ++49-(0)211-811-2408 | | Fax: ++49-(0)211-811-2333 | | e-mail: schuessl@uni-duesseldorf.de | --------------------------------------------- From owner-7tms_r@net.bio.net Fri Mar 29 22:00:00 1996 Path: biosci!ITSA.UCSF.EDU!zjwang From: zjwang@ITSA.UCSF.EDU (Zhaijie Wang) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 30 Mar 1996 20:05:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603310403.UAA188532@itsa.ucsf.EDU> NNTP-Posting-Host: net.bio.net subscribe z wang