From owner-7tms_r@net.bio.net Sat May 04 23:00:00 1996 Path: biosci!ARGOTECH.COM!pvaneikeren From: pvaneikeren@ARGOTECH.COM (Paul van Eikeren) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 1996 GRC Biocatalysis Date: 5 May 1996 17:07:39 -0700 Organization: Argonaut Technologies Inc. Lines: 174 Sender: daemon@net.bio.net Distribution: world Message-ID: <318D4210.728@argotech.com> NNTP-Posting-Host: net.bio.net =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Gordon Research Conference B I O C A T A L Y S I S Kimbal Union Academy * Meriden, NH, USA * 7-12 July 1996 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D http://w3.argotech.com/argotech/biocatalysis I am posting this message to inform you of this years=92 Gordon Research = Conference on Biocatalysis. The Conference will be held 7-12 July 1996 = at Kimbal Union Academy in Meriden, New Hampshire, USA. The Conference = brings together an interdisciplinary group of biologists, chemists, and = engineers for a full week of intense discussion and examination of the = frontiers of Biocatalysis. We welcome your application for = participation in year=92s conference. Also, we would be grateful if you = would forward a copy of this message to people that you think might be = interested in attending. The subject of the Biocatalysis Conference is synthetically useful = reactions and processes catalyzed by enzymes or whole cells. We will = address three main themes: =B7 new uses of existing enzymes (e.g., lipases, oxidases, and aldolases); =B7 discovery of new enzymes (e.g., epoxide hydrolases, P-450 = hydroxylases, oxynitrilases, thermophilic organisms); and =B7 structure and protein engineering of enzymes (e.g., new structures of = proteases, transketolase and oxynitrilase, and combinatorial methods = versus site-directed mutagenesis). = We have assembled a notable group of speakers, discussion leaders, and = poster presenters. Attached is a copy of the preliminary program. For = additional information on the Conference including the most recent = update of the program and the poster sessions we suggest that you = connect to our World-Wide Web site at http://w3.argotech.com/argotech/biocatalysis If you do not have access to the World-Wide Web or need additional = information, please contact me by e-mail at the address shown below and = can send you additional information and applications forms. We look forward to receiving your application to the conference. Sincerely, Paul van Eikeren =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Paul van Eikeren, Co-Chair Vice President, Chemistry Argonaut Technologies Inc. 887 Industrial Road, Suite G San Carlos, CA 94070 USA Voice: +1 415 598 1350 ext. 217 Fax: +1 415 598 1359 pvaneikeren@argotech.com 74260.1024@compuserve.com =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D P R O G R A M I N F O R M A T I O N (Updated on 25 March 1996) =3D=3D Opening Session: * Stanley Roberts (Liverpool, UK) Enzymic Baeyer Villiger Reaction * J. John Holbrook (U. Bristol, UK) Getting the Products Off Enzymes = Used in Bulk Chemoenzymic Synthesis =3D=3D Structure and Engineering of Hydrolases for Organic Synthesis * Sabine L. Flitsch (U. Edinburgh, UK) Design and Synthesis of = Enzyme-Cleavable Linkers for Solid Phase Synthesis * Guy G. Dodson (York U., UK) Nucleophilic Attack at the Carbonyl in = Hydrolases: The Varying Stereochemistry at the Nucleophile * Franz Effenberger (U. Stuttgart, Germany) New Results on Preparation = and Application of Chiral Cyanohydrins * Robert Menard (NRC Biotechnology Research Institute, Canada) Protein = Engineering of Cysteine Proteases: From a Better Understanding of Their = Function to Redesigning the Catalytic Activity =3D=3D Molecular Evolution of Subtilisin * Frances Arnold (California Institute of Technology, USA) Directed = Evolution of p-Nitrobenzyl Esterase * Marcus Ballinger (Genentech, USA) Subtilisin BPN Variants Designed for = Cleavage of Multibasic Substrates Multibasic Substrates = * Volker Schellenberger (Genencor, USA) Directed evolution of a = Bacillus protease =3D=3D New Application of Hydrolases * Herbert Waldmann (Karlsruhe, Germany) Bioorganic Synthesis and = Biological Signal Transduction * Milton Zmijewski (Lily, Indianapolis, USA) Enzymatic Removal of = Protecting Groups in the Synthesis of Pharmaceuticals * Kazuo Achiwa (Shizuoka U.) Lipase-Catalyzes Asymmetric Synthesis of = Optically-Active Medicines: New Strategies and their Application =3D=3D Discovery versus Engineering of New Enzymes * John Arnett (Recombinant Biocatalysis, USA) Enzymes from Thermophilic = Microorganisms * Gunter Schneider (Karolinska Institute, Stockholm, Sweden) Toward = Tailoring Enzymes for Asymmetric Synthesis: Protein Engineering of = Transketolase =3D=3D Aldolases and Glycosyl Transfer * Eric Toone (Duke University) Pyruvate Aldolases * Vladimir Kren (Czech Academy of Sciences) Enzymatic Glycosylation of = Pharmacologically Active Compounds: Multienzymatic Approaches and New = Enzymes =3D=3D New Hydrolases * Roland Furstoss (U. Aix-Marseille, France) Enantioselective = Biotransformations with Epoxide Hydrolases * Kenji Soda (Kyoto, Japan) 2-Haloacid Dehalogenases: Their Functions, = Structures, and Applications =3D=3D Oxidations * Aleksey Zaks (Schering-Plough, Union, NJ, USA) Chloroperoxidase * Bernhard Hauer (BASF AG, Ludwigshafen, Germany) Selection of = Biocatalysts for the Preparation of Chiral/Chemical Building Blocks * Roger Sheldon (Delft U. Netherlands) Enantioselective Aminolysis and = Selective Oxidations Mediated by Chloroperoxidase =3D=3D Large Scale Industrial Applications * Kevin DiGregorio (Union Carbide, USA) Polyester Hydrolysis * Robert Dicosimo (Dupont, Wilmington, DE, USA) Scale-Up of a = Biocatalytic Route to N-Phosphonomethylglycine (Glyphosate) Using Whole = Cell Transformants From owner-7tms_r@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: bertin@cochin.inserm.fr (Brigitte BERTIN) Newsgroups: bionet.molbio.proteins.7tms_r Subject: R-G coupling selectivity Date: 7 May 1996 09:27:59 +0100 Lines: 22 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4mn1if$2qd@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk Reply to P. Butkerait The question of coupling selectivity at the molecular level is not yet answered. Several papers of good quality demonstrated clearly that alpha, beta and gamma are all involved in R-G coupling selectivity but results about which G alpha or beta/gamma subunit is doing what are sometimes contradictory. For example (Raymond et al., Biochem.32:11064-73,1993) and (Bertin et al., JBC 267:8200-06, 1992) found that Gialpha3 had the best coupling affinity to human 5-HT1A although (butkerait et al., JBC 270:18691-99, 1995) found no difference between Gialpha subtypes. All these protocols contained at least one possible biais; respectively antibodies affinity against the different alpha subtypes, the absence of recombinant Galpha myristoylation in coli and the use of different beta/gamma dimers in each system. I think that beta/gamma selectivity could participate in G alpha selectivity and above all modify the range of coupling selectivity to Galpha's. We used beta/gamma purified from bovine brain to reconstitute the coupling to rGalpha, which were not equivalent to beta/gamma from Hela cells and to beta 1/gamma2 expressed in Sf9. Thus, to address the question of R-G coupling selectivity, it may be necessary not to dissociate alpha from beta/gamma and to try different experimental systems. From owner-7tms_r@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!newsfeed.internetmci.com!sgigate.sgi.com!nntp.coast.net!oleane!jussieu.fr!citi2.fr!camoin.cochin.inserm.fr!user From: brydon@icgm.cochin.inserm.fr (Lena Brydon) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Review GPCR-Antibody Production Date: Tue, 07 May 1996 17:48:04 +0200 Organization: icgm cnrs upr415 Lines: 26 Message-ID: NNTP-Posting-Host: camoin.cochin.inserm.fr X-Newsreader: Yet Another NewsWatcher F2.0 >Dear Netters, > >I am compiling a review of existing and proposed methods for the >production and utilisation of specific antibodies against G-protein >coupled receptors. > >I would be very grateful for any information/ideas you have regarding this >subject area. >If anyone has any papers recently published or in Press regarding this >subject and are willing to send me re-prints, I will of course quote these >authors in the review ( if so desired.) The results of this inquiry will be sent to this news group later. Thanks to all those who have already responded!(Especially Keld Sorensen) The following is my snail mail address, for anyone else who would like to send me new ideas/info/re-prints: Lena Brydon, ICGM, CNRS UPR415, 22 rue Mechain-75014 PARIS, FRANCE. Fax.(331) 40.51.72.10. From owner-7tms_r@net.bio.net Sat May 11 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 12 May 1996 02:01:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605120900.CAA23515@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-7tms_r@net.bio.net Sun May 12 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!ra.nrl.navy.mil!news.math.psu.edu!news.cse.psu.edu!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!news.dacom.co.kr!news.kreonet.re.kr!usenet.etri.re.kr!usenet.kornet.nm.kr!snunews.snu.ac.kr!NewsWatcher!user From: juhnn@plaza.snu.ac.kr (YONG-SUNG JUHNN) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Asking receptors expressed on COS cell membrane Date: Mon, 13 May 1996 18:27:59 +0900 Organization: DEPT OF BIOCHEMSITRY, SNU COLLEGE OF MEDICINE Lines: 10 Message-ID: NNTP-Posting-Host: 147.46.161.102 Hi, I am planning to work on signal transduction with COS-1 cell line. I need to know what kinds of membrane receptors are endogeneously expressed on this cell line or COS-7 cells. I would be grateful, if you can tell me the expressed receptor types and the related reference by e.mail. My address is juhnn@plaza.snu.ac.kr Thank you. From owner-7tms_r@net.bio.net Sun May 12 23:00:00 1996 Path: biosci!ITSA.UCSF.EDU!barber From: barber@ITSA.UCSF.EDU (Diane Barber) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Ltk- fibroblasts Date: 13 May 1996 18:18:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: Diane Barber NNTP-Posting-Host: net.bio.net Looking for list of endogenous receptors expressed in Ltk- fibroblasts. I think these are derived from L cells Many thanks Diane Barber From owner-7tms_r@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!MOLDEV.COM!martin_cunningham From: martin_cunningham@MOLDEV.COM (Martin Donal Cunningham, Ph.D.) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 14 May 1996 13:14:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Is anyone aware of a publication that lists endogenous receptors in different cell lines? Martin Donal Cunningham, Ph.D. Molecular Devices Corporation 1311 Orleans Dr. Sunnyvale, Calif. 94089 Direct: 408-747-3524 Fax: 408-747-3601 From owner-7tms_r@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: (none) Date: Wed, 15 May 1996 10:15:52 -0400 Organization: University of Michigan Lines: 20 Message-ID: <3199E718.7FFC@umich.edu> References: NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) To: "Martin Donal Cunningham, Ph.D." Nartin, Agi Schonbrun at UT Houston has begun compling such a list. I don't think there is an official publication but I'd be willing to put a file with that info up on a web server if someone made up the file. Rick http://www-personal.umich.edu/~rneubig Martin Donal Cunningham, Ph.D. wrote: > > Is anyone aware of a publication that lists endogenous receptors in > different cell lines? > > Martin Donal Cunningham, Ph.D. > Molecular Devices Corporation > 1311 Orleans Dr. > Sunnyvale, Calif. 94089 > Direct: 408-747-3524 > Fax: 408-747-3601 From owner-7tms_r@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!FARMR1.MED.UTH.TMC.EDU!aschonb From: aschonb@FARMR1.MED.UTH.TMC.EDU (A. Schonbrunn) Newsgroups: bionet.molbio.proteins.7tms_r Subject: endogenous receptors Date: 15 May 1996 08:51:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 50 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Several people have recently asked about endogenous receptors expressed in cell lines commonly used for transfection. I have compiled such a list (attached) and would appreciate any additions/corrections/updates from this group. I will post an updated list on our web server as soon as I get a chance. Agi Schonbrunn CHO-K1 - beta adrenergic receptors: =89 30 fmol/mg protein - thrombin receptors coupled to PLC - CCK coupled to PLC - controversial! - alpha2-adrenergic receptors (<40 fmol/mg). - calcitonin receptors - type I VIP receptors - ATP coupled to Ca++ mobilisation (at 10-5 M) HEK-293 - beta adrenergic receptors: =89 7-20 fmol/mg protein - coupled to cyclase stimulation (PNAS 89:9809, 1992) - somatostatin receptors coupled to cyclase inhibition (FEBS Lett 331:53, 1993) - muscarinic receptors coupled to: - stimulation of PLC (JBC 267:31, 1992) and - inhibition of cylcase (JBC 267: 24858, 1992) - a thrombin receptor - UTP (P2u) receptor - PGE EP2 receptor - adenosine A2b receptor - lysophosphatidic acid receptor COS - beta adrenergic receptors - thrombin receptor - UTP (P2u) receptor - PGE EP2 receptor - adenosine A2b receptor - lysophosphatidic acid receptor Ltk- - P2u (UTP) receptor (PLC & Ca) ************************************************************************** Agi Schonbrunn, Ph.D. e-mail: aschonb@farmr1.med.uth.tmc.edu Professor, Dept. of Pharmacology, Univ. of Texas Medical School P.O.Box 20708, Houston,TX 77225 From owner-7tms_r@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!CHEM.VU.NL!gerhardt From: gerhardt@CHEM.VU.NL (Cindy Gerhardt) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unknown signal transduction Date: 17 May 1996 05:42:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does someone out there know if there are still GPCRs of which no signal transduction pathway has been identified (yet)? Many thanks! Cindy Gerhardt Dept. Biochemistry and molecular biology Fac. Chemistry Vrije Universiteit Amsterdam The Netherlands From owner-7tms_r@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!CHEM.VU.NL!gerhardt From: gerhardt@CHEM.VU.NL (Cindy Gerhardt) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unknown signal transduction Date: 17 May 1996 06:17:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does someone out there know if there are still GPCRs of which no signal transduction pathway has been identified? Many thanks! Cindy Gerhardt Dept. Biochemistry and molecular biology Fac. Chemistry Vrije Universiteit Amsterdam The Netherlands From owner-7tms_r@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!NEWSSUN.MED.MIAMI.EDU!vslepak From: vslepak@NEWSSUN.MED.MIAMI.EDU ("Vladlen Z. Slepak") Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe 7tms_r Date: 17 May 1996 14:16:02 -0700 Organization: University of Miami Medical School Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <319CEBF0.51A4@newssun.med.miami.edu> Reply-To: vslepak@newssun.med.miami.edu NNTP-Posting-Host: net.bio.net subscribe 7tms_r From owner-7tms_r@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!SEQAXP.BIO.CALTECH.EDU!VSLEPAK From: VSLEPAK@SEQAXP.BIO.CALTECH.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe 7tms_r Date: 17 May 1996 14:21:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <960517141804.20200486@seqaxp.bio.caltech.edu> NNTP-Posting-Host: net.bio.net unsubscribe 7tms_r From owner-7tms_r@net.bio.net Fri May 17 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!fu-berlin.de!news.belwue.de!news.uni-ulm.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!uni-regensburg.de!lrz-muenchen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: unknown signal transduction Date: 17 May 1996 14:57:45 GMT Organization: University of Wuerzburg, Germany Lines: 23 Distribution: world Message-ID: <4ni459$60b@winx03.informatik.uni-wuerzburg.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Cindy Gerhardt (gerhardt@CHEM.VU.NL) wrote: > Does someone out there know if there are still GPCRs of which no signal > transduction pathway has been identified (yet)? I would believe that for orphan receptors the signal transduction pathways are generally not known. There are most likely also receptors with known ligands for which the signal transduction pathway is not known since there are very recent papers about the elucidation of specific receptor-G-protein coupling. One example are the odorant receptors where it is known that they can couple to cyclase or PLC, but it is often not known which receptor couples to which effector. One of the problems seems to be that it is very difficult, if not impossible, to determine from the sequence to which effector or G-protein a certain receptor couples. Hope that helps, --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun May 19 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!swrinde!cssun.mathcs.emory.edu!news.cc.emory.edu!usenet From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: unknown signal transduction Date: Mon, 20 May 1996 10:32:26 -0700 Organization: Emory University Lines: 13 Message-ID: <31A0ACAA.7346@bimcore.emory.edu> References: NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I; 16bit) To: Cindy Gerhardt Cindy Gerhardt wrote: > > Does someone out there know if there are still GPCRs of which no signal > transduction pathway has been identified (yet)? > The AT2 angiotensin "receptor" falls in here. It binds angiotensin with very high (1 nM) affinity but what happens next is enigmatic. An awful lot of signalling-like events have been ascribed to it, but the evidence for these is conflicting and controversial. There is no (good) evidence for coupling to PLC or adenylyl cyclase. Now, where did I put that fire exstinquisher.... From owner-7tms_r@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!news2.EUnet.fr!Belgium.EU.net!news From: Jurgen Vanhauwe Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: unknown signal transduction Date: 23 May 1996 07:50:35 GMT Organization: Janssen Pharmaceutica Lines: 7 Message-ID: <4o15cb$9a3@news.Belgium.EU.net> References: NNTP-Posting-Host: pc4.cim.jrf.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: gerhardt@CHEM.VU.NL The dopamine D3 receptor is also quite ambigous concerning signal transduction. Some effects have been described (e.g. effects on adenylyl cyclase or K+ channels), but we don't know exactly to which G proteins this D3 receptor is coupling. Jurgen From owner-7tms_r@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Ron Taussig Newsgroups: bionet.molbio.proteins.7tms_r Subject: (no subject) Date: 23 May 1996 20:23:08 GMT Organization: University of Michigan Lines: 14 Message-ID: <4o2hfc$o52@lastactionhero.rs.itd.umich.edu> NNTP-Posting-Host: host-140.subnet-35.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.molbio.proteins.7tms_r Post Doctoral Position--two years of support are available for a joint University of Michigan/Parke-Davis collaboration to study aspects of G protein coupled signaling. The position is targeted for candidates with less than 2 years of post doctoral training and is available immediately. The project will be carried out in the laboratories of Ron Taussig (Department of Biological Chemistry, U of Michigan) and Robert MacKenzie (Department of Cell Biology, Parke-Davis). Applicants should send CV including statement of research interests and references to: Ron Taussig, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-0636. For further information email taussig@umich.edu (web site = http://www.med.umich.edu/biochem/all.faculty/taussig.html) or mackenr@aa.wl.com From owner-7tms_r@net.bio.net Sun May 26 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.dacom.co.kr!bofh.dot!usenet.seri.re.kr!bofh.dot!xpat.com!usenet.kornet.nm.kr!snunews.snu.ac.kr!NewsWatcher!user From: juhnn@plaza.snu.ac.kr (YONG-SUNG JUHNN) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Summary of endogeous membrane receptors in COS, CHO etc. cells Date: Tue, 28 May 1996 10:48:20 +0900 Organization: DEPT OF BIOCHEMSITRY, SNU COLLEGE OF MEDICINE Lines: 59 Message-ID: NNTP-Posting-Host: 147.46.161.102 Hi, This is the list compiled from the response that I received to my original quiery about endogeneous membrane receptors. Thanks to those who replied. If you like to know the source of the answer, please write to me. juhnn@plaza.snu.ac.kr ************************************* COS Cells - beta adrenergic receptors - thrombin receptor - UTP (P2u) receptor - PGE EP2 receptor - adenosine A2b receptor - lysophosphatidic acid receptor - D1-like dopamine receptors (COS-1): low density - somatostatin - muscarinic - EGF CHO-K1 - beta adrenergic receptors: 30 fmol/mg protein - thrombin receptors coupled to PLC - CCK coupled to PLC - alpha2-adrenergic receptors (<40 fmol/mg). - calcitonin receptors - type I VIP receptors - ATP coupled to Ca++ mobilisation (at 10-5 M) - 5HT - PAF HEK293 - beta adrenergic receptors: 7-20 fmol/mg protein - somatostatin receptors coupled to cyclase inhibition - muscarinic receptors coupled to: - stimulation of PLC (JBC 267:31, 1992) and - inhibition of cylcase (JBC 267: 24858, 1992) - a thrombin receptor - UTP (P2u) receptor - PGE EP2 receptor - adenosine A2b receptor - lysophosphatidic acid receptor - Endothelin - ATP L(tk-) - P2u (UTP) receptor (PLC & Ca) Adenosine (?), ATP Hela Endothelin (?), Isoproterenol (?), Carbachol, ATP 3T3 Endothelin, Adenosine (?), Isoproterenol, ATP ******************************************************* juhnn@plaza.snu.ac.kr