From owner-7tms_r@net.bio.net Thu Aug 01 23:00:00 1996 Path: biosci!OCELOT.RUTGERS.EDU!PDU From: PDU@OCELOT.RUTGERS.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: MS Chemists Wanted Date: 2 Aug 1996 05:58:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I7SM5T8ZM88Y4Y2C@mbcl.rutgers.edu> NNTP-Posting-Host: net.bio.net Dear All, Synaptic Pharmaceutical has multiple openings for chemists with Master's degree in synthetic organic chemistry. Experience with HPLC, automated lab equipment, and computer data analysis would be helpful. Interested persons please send CV to the following address or this email address. Dr. Mohammad Mazarbadi Department of Chemistry Synaptic Pharmaceutical Corporation 215 College Road Paramus, NJ 07652 From owner-7tms_r@net.bio.net Sun Aug 04 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: vijay@bmc.uu.se (Vijay Chhajlani) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Receptor:G-proteins Date: 5 Aug 1996 08:56:58 +0100 Lines: 13 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4u49ga$1s8@mserv1.dl.ac.uk> X-Sender: vijay@bio.embnet.se Original-To: 7tms_r@dl.ac.uk Does any one know of a compiled list dscribing the different G protein coupled receptors and the corresponding G proteins. If I rceive partial lists, I will compile them and post. Vijay Chhajlani Tel: +46 18 17 4103/4930 Associate Professor Fax: +46 18 501920 Pharmaceutical Pharmacology Division Biomedical Centre, Box 591, Uppsala 751 24 sweden From owner-7tms_r@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!cssun.mathcs.emory.edu!news.service.emory.edu!news From: KPM Newsgroups: bionet.molbio.proteins.7tms_r Subject: Is anybody using two hybrid system for specificity of receptor coupling to alpha subunits? Date: Tue, 06 Aug 1996 14:49:12 -0700 Organization: Emory University Lines: 7 Message-ID: <3207BDD8.BFE@pharm.emory.edu> NNTP-Posting-Host: minnpc.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Has anyone tried the yeast two hybrid system for examining the specificity of coupling of GPCRs to alpha subunits? I would appreciate any references and/or experience anyone has had in this area. Thanks Ken Minneman From owner-7tms_r@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!biosci!not-for-mail From: a.c.noorlandt@chem.ruu.nl (Albert Noorlandt) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.immunology,bionet.metabolic-reg,bionet.molbio.ageing,bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,alt.med,alt.pharm,sci.bio.misc,sci.chem,sci.chem.analytical,sci.med,sci.misc,sci.techniques Subject: 1997 FEBS Meeting on Cell Signalling Mechanisms Date: 6 Aug 1996 17:24:42 -0700 Organization: CBLE Lines: 29 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:2198 bionet.cellbiol:5225 bionet.immunology:9491 bionet.metabolic-reg:805 bionet.molbio.ageing:2916 bionet.molbio.methds-reagnts:47700 bionet.molbio.proteins:8451 bionet.molbio.proteins.7tms_r:804 sci.bio.misc:4117 sci.chem:61653 sci.chem.analytical:5118 sci.med:133749 sci.misc:12601 FEBS Special Meeting on Cell Signalling Mechanisms, From membrane to nucleus Amsterdam, The Netherlands, June 29­July 3, 1997 Everything you want to know about this meeting on www-page: http://www.cble.chem.ruu.nl/febs97/index.html The meeting will cover the following topics: € Tyrosine kinases € MAP-, Ser/Thr-kinases € G-coupled receptors € Lymphokine receptors € Ras, Rac, Rho-proteins € Ca2+, cGMP, NO € Lipid signalling € Steroid receptors € Transcription factors € Cell cycle, Apoptosis For further information contact: Secretariat FEBS Special Meeting 1997 Lidy Groot Congress Events P.O. Box 83005 1080 AA Amsterdam The Netherlands Tel. +31.20.679 3218 Fax: +31.20.675 8236 Email: lidy.groot@inter.nl.net From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Freddy de Bree" Newsgroups: bionet.molbio.proteins.7tms_r Subject: N-glycosylation Date: 8 Aug 1996 15:07:29 +0100 Lines: 12 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ucsb1$hfp@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk Dear Harm (Beste Harm), To my knowledge the cannabinoid receptor is also devoid of a glycosylation signal and can be expressed in Sf cells and is endogenously expressed in NG108 neuroblastoma cells (if the name is correct). Regards, Freddy De Bree Protein Structure Group Dept Chemistry University of York From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: N-linked glycosylation Date: 8 Aug 1996 06:38:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Harm van Heerikhuizen (vheerik@chem.vu.nl( wrote: >Dear netters, > >We have recently cloned a GPCR that seems not to contain any consensus >sites for N-linked glycosylation in its extracellular domains. >A large body of published data indicates that N-linked glycosylation is >important for proper routing and high-level expression of GPCRs. >However, despite the fact that our receptor cannot be glycosylated it is >expressed at high levels in HEK293 cells. > >Is anyone aware of any other GPCR (published or unpublished) that is also >devoid of N-linked glycosylation? The alpha2b adrenergic has been so reported (Weinshank et al. 1990 Mol. Pharm. 38:681-688; Lomansey et al. 1990 PNAS 87:5094-5098), as has an orphan melatonin-related receptor (Reppert et al., 1996 FEBS Lett. 386:219-224). Cheers, AJW ------------------------------------------------------------------------ __ __ __ A. John Watson, Ph.D. /__/__/__/\ Bristol-Myers Squibb Company /__/__/__/\/\ Pharmaceutical Reseach Institute /__/__/__/\/\/\ CNS Molecular Biology, Dept. 405 \__\__\__\/\/\/ 5 Research Parkway \__\__\__\/\/ Wallingford, CT 06492 \__\__\__\/ watson_j@bms.com | 203.284.6745 voice | Standard Disclaimers Apply 203.284.7569 fax | ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!war.wyeth.com!hadcocj From: hadcocj@war.wyeth.com (John Hadcock) Newsgroups: bionet.molbio.proteins.7tms_r Subject: N-linked glycosylation -Reply Date: 8 Aug 1996 06:03:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Harm The human alpha2b-adrenergic receptor does not contain any consensus sequences for N-linked glycosylation. Also, we truncated the rat somatostatin receptor subtype 2 which removes all putative N-glycosylation sites in the extracellular domains. We found that the receptor is still expressed in high amounts with pharmacology indistinguishable from the wild-type receptor. Also work from Lefkowitz's and Malbon's labs many years ago reported that N-linked glycosylation of the beta2-adrenergic receptor may not be critical for proper routing of this receptor. John Hadcock Wyeth-Ayerst Reseearch hadcocj@war.wyeth.com From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!CHEM.VU.NL!vheerik From: vheerik@CHEM.VU.NL (Harm van Heerikhuizen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: N-linked glycosylation Date: 8 Aug 1996 04:07:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear netters, We have recently cloned a GPCR that seems not to contain any consensus sites for N-linked glycosylation in its extracellular domains. A large body of published data indicates that N-linked glycosylation is important for proper routing and high-level expression of GPCRs. However, despite the fact that our receptor cannot be glycosylated it is expressed at high levels in HEK293 cells. Is anyone aware of any other GPCR (published or unpublished) that is also devoid of N-linked glycosylation? Thanks Harm van Heerikhuizen Molecular Neurobiology group Dept. of Biochemistry & Mol. Biol. Vrije Universiteit, Amsterdam The Netherlands From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!Gems.VCU.EDU!DPETTIT From: DPETTIT@Gems.VCU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: glycosylation Date: 8 Aug 1996 09:18:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I816JZRY4I929QD0@Gems.VCU.EDU> NNTP-Posting-Host: net.bio.net Original-To: 7tms_r@dl.ac.uk Dear Harm (Beste Harm), To my knowledge the cannabinoid receptor is also devoid of a glycosylation signal and can be expressed in Sf cells and is endogenously expressed in NG108 neuroblastoma cells (if the name is correct). Regards, Freddy De Bree Protein Structure Group Dept Chemistry University of York Dear Dr. De Bree, The neural cannabinoid receptor (CB1) has three potential glycosylation sites (Matsuda et al. Nature Vol 346:561-564) in the extracellular amine terminus. It is thought that only two of these sites are actually glycosylated. For more detailed information see: Chao Song and Allyn C. Howlett "Rat brain cannabinoid receptors are n-linked glycosylated proteins", Life Science Vol 56:1983-1995. As for the peripheral cannabinoid receptor (CB2), there is one potential glycosylation site in the extracellular amine terminus. I don't know of any studies that have addressed it's use. Regards, Denise Pettit Medical College of Virginia From owner-7tms_r@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: leurs@chem.vu.nl (Rob Leurs) Newsgroups: bionet.molbio.proteins.7tms_r Subject: TSH receptor Date: 8 Aug 1996 11:04:49 +0100 Lines: 19 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4uce41$fr@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk I am looking for the TSH receptor cDNA and the Graves IgG receptor autoantibodies. Can someone supply us with these items? Your help is appreciated! Rob ****************************************************************************** Rob Leurs, Ph.D Leiden/Amsterdam Center for Drug Research Division of Medicinal Chemistry Department of Pharmacochemistry, Vrije Universiteit De Boelelaan 1083, 1081 HV Amsterdam the Netherlands fax: 31204447610 tel: 31204447579 leurs@chem.vu.nl From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!howland.erols.net!newsserver.jvnc.net!netnews.sbphrd.com!steven_mcclue-1 From: steven_mcclue-1@sbphrd.com (Steve McClue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Receptor:G-proteins Date: Fri, 09 Aug 1996 11:49:10 +0000 Organization: SB Pharmaceuticals Lines: 18 Distribution: bionet Message-ID: References: <4u49ga$1s8@mserv1.dl.ac.uk> NNTP-Posting-Host: ham992.ha.uk.sbphrd.com X-Newsreader: Yet Another NewsWatcher 2.2.0b6 In article <4u49ga$1s8@mserv1.dl.ac.uk>, vijay@bmc.uu.se (Vijay Chhajlani) wrote: > Does any one know of a compiled list dscribing the different G protein > coupled receptors and the corresponding G proteins. > If I rceive partial lists, I will compile them and post. > > Vijay Chhajlani Tel: +46 18 17 4103/4930 There's a long list (probably a bit out of date now) which would be a good place to start in the monster review by Lutz Birnbaumer in Biochem Biophys Acta 1031 (1990) 163-224. Steve -- My opinions, not my employer's. From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Freddy de Bree Newsgroups: bionet.molbio.proteins.7tms_r Subject: G protein Date: 9 Aug 1996 16:09:44 +0100 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ufkbo$h9f@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk > coupled receptors and the corresponding G proteins Dear Vijay Chhajlani, there is a nice book that reviews a awful lot of GPCRs and their G-prot. It is available with Academic Press. Freddy From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!ASPIRINE.U-STRASBG.FR!weill From: weill@ASPIRINE.U-STRASBG.FR (Claire Weill) Newsgroups: bionet.molbio.proteins.7tms_r Subject: hmAChR Date: 9 Aug 1996 00:43:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <320B085D.7786@pharma.u-strasbg.fr> NNTP-Posting-Host: net.bio.net For our research in muscarinic acetylcholine receptor pharmacology, we're looking for human m1 and/or m2 AChR cDNA. Is there any possibility to get/buy this DNA preferably included in a plasmid for expression in eucaryotic cells, from a company or a laboratory ? Please contact us if you have any information. Yours sincerely. Claire Weill Laboratoire de Chimie BioOrganique CNRS URA 1386 STRASBOURG-FRANCE TEL : 33-88-67-69-60 FAX : 33-88-67-88-91 From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!pasteur.fr!jussieu.fr!univ-angers.fr!ciril.fr!u-strasbg.fr!mgpwmac2.u-strasbg.fr!weill From: Claire Weill Newsgroups: bionet.molbio.proteins.7tms_r Subject: looking for hm1AChR cDNA Date: 9 Aug 1996 09:41:16 GMT Organization: Chimie Bioorganique Lines: 16 Distribution: world Message-ID: <4uf13s$gm1@news.u-strasbg.fr> NNTP-Posting-Host: mgpwmac2.u-strasbg.fr Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_PPC X-XXMessage-ID: X-XXDate: Fri, 9 Aug 1996 10:39:20 GMT For our research in muscarinic acetylcholine receptor pharmacology, we're looking for human m1 and/or m2 AChR cDNA. Is there any possibility to get/buy this DNA preferably included in a plasmid for expression in eucaryotic cells, from a company or a laboratory ? Please contact us if you have any information. Yours sincerely Claire Weill Laboratoire de Chimie Bioorganique CNRS URA 1386 STRASBOURG FRANCE Tel : 33-88-67-69-60 Fax : 33-88-67-88-91 Email : weill@pharma.u-strasbg.fr From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!agate!howland.erols.net!usc!usenet From: yongyang@scf.usc.edu (yongyang) Newsgroups: bionet.molbio.proteins.7tms_r Subject: test Date: 9 Aug 1996 23:21:11 GMT Organization: University of Southern California, Los Angeles, CA Lines: 1 Sender: yongyang@socialwork-pc3.usc.edu Message-ID: <4ugh57$b5a@usc.edu> NNTP-Posting-Host: socialwork-pc3.usc.edu testing From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!agate!howland.erols.net!usc!usenet From: yongyang@scf.usc.edu (yongyang) Newsgroups: bionet.molbio.proteins.7tms_r Subject: testing Date: 9 Aug 1996 23:23:23 GMT Organization: University of Southern California, Los Angeles, CA Lines: 1 Sender: yongyang@socialwork-pc3.usc.edu Message-ID: <4ugh9b$b5a@usc.edu> NNTP-Posting-Host: socialwork-pc3.usc.edu dgddfdf From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: G protein Date: 9 Aug 1996 10:51:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Freddy de Bree wrote: >> coupled receptors and the corresponding G proteins > >Dear Vijay Chhajlani, > >there is a nice book that reviews a awful lot of GPCRs and their G-prot. >It is available with Academic Press. > >Freddy He is refering to the _G_Protein Linked Receptor FactsBook_, by S. Watson and S. Arkinstall (Academic Press, 1994). I like it, too, though by now it is out of date. AJW From owner-7tms_r@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!UMDNJ.EDU!howells From: howells@UMDNJ.EDU (Richard Howells) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: N-linked glycosylation (fwd) Date: 9 Aug 1996 13:17:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608092017.NAA22143@net.bio.net> NNTP-Posting-Host: net.bio.net Forwarded message: > From howells@UMDNJ.EDU Fri Aug 9 16:09:08 EDT 1996 > From: Richard Howells > Subject: Re: N-linked glycosylation > To: vheerik@chem.vu.nl (Harm van Heerikhuizen) > Date: Fri, 9 Aug 1996 16:09:05 -0400 (EDT) > Cc: howells@UMDNJ.EDU (Richard Howells) > In-Reply-To: from "Harm van Heerikhuizen" at Aug 8, 96 04:07:47 am > X-Mailer: ELM [version 2.4 PL25] > Mime-Version: 1.0 > Content-Type: text/plain; charset=US-ASCII > Content-Transfer-Encoding: 7bit > Content-Length: 1557 > > > > > Dear netters, > > > > We have recently cloned a GPCR that seems not to contain any consensus > > sites for N-linked glycosylation in its extracellular domains. > > A large body of published data indicates that N-linked glycosylation is > > important for proper routing and high-level expression of GPCRs. > > However, despite the fact that our receptor cannot be glycosylated it is > > expressed at high levels in HEK293 cells. > > > > Is anyone aware of any other GPCR (published or unpublished) that is also > > devoid of N-linked glycosylation? > > > > Thanks > > > > Harm van Heerikhuizen > > Molecular Neurobiology group > > Dept. of Biochemistry & Mol. Biol. > > Vrije Universiteit, Amsterdam > > The Netherlands > > > > > Harm: > > In response to your question, we have created a truncated form of the mu > opioid receptor that lacks all N-linked glycosylation sites and this receptor > is expressed at high levels in HEK293 cells and binds ligand with normal > affinity (Shahrestanifar et al., J. Biol. Chem. 271: 5505-5512, 1996). We do > not know whether the receptor is localized on the plasma membrane, since we > generally use a crude membrane preparation for binding studies. Thus, although > this is not a natural example of a non-glycosylated receptor, it is expressed > well. There are examples of naturally occuring receptors that lack consensus > N-linked glycosylation sites: human and rat alpha2B adrenergic receptors and > the adenosine A2 receptor.> > > Richard Howells > Department of Biochemistry and Molecular Biology > University of Medicine and Dentistry of New Jersey > Newark, NJ USA > > > > From owner-7tms_r@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Aug 1996 02:00:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608120900.CAA20576@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!GUITAR.ROCKEFELLER.EDU!ilya From: ilya@GUITAR.ROCKEFELLER.EDU (Ilya Vakser) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Announcement - protein docking program GRAMM Date: 12 Aug 1996 15:44:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 93 Sender: daemon@net.bio.net Distribution: world Message-ID: <320FB51A.59E2@guitar.rockefeller.edu> NNTP-Posting-Host: net.bio.net ----------- GRAMM v1.03 ----------- This note is to announce the availability of GRAMM (Global RAnge Molecular Matching) software. GRAMM is a program for protein docking. To predict the structure of a complex, it requires only the atomic coordinates of the two molecules (no information about the binding sites is needed). The program performs an exhaustive 6-dimensional search through the relative translations and rotations of the molecules. The molecular pairs may be: two proteins, a protein and a smaller compound, two transmembrane (TM) helices, etc. GRAMM may be used for high- resolution molecules, for inaccurate structures (where only the gross structural features are known), in cases of large conformational changes, etc. The Global Range Molecular Matching (GRAMM) methodology is an empirical approach to smoothing the intermolecular energy function by changing the range of the atom-atom potentials. The technique allows to locate the area of the global minimum of intermolecular energy for structures of different accuracy. The quality of the prediction depends on the accuracy of the structures. Thus, the docking of high-resolution structures with small conformational changes yields an accurate prediction, while the docking of ultralow-resolution structures will give only the gross features of the complex. _________________ I am making GRAMM publicly available following a number of requests from different labs. I would like to make it clear, however, that both the methodology and the program, at present, are in the process of active development and validation, especially in the area of the low-resolution docking, and have to be viewed like that. The program is free. However, I would expect proper references. I will also appreciate bug reports. To get GRAMM, send the request to ilya@guitar.rockefeller.edu. The GRAMM site on the Web is http://guitar.rockefeller.edu/. GRAMM was created during my stay in the Weizmann Institute (1991-1993), in the Washington University (1993-1995), and in the Rockefeller University (1995-1996). I deeply appreciate the assistance of my colleagues in all these institutions (especially, Profs. Ephraim Katchalski-Katzir, Garland Marshall, and Andrej Sali). Platforms Presently, GRAMM is compiled on SGI R4000, SGI R4400, SGI R8000, and SGI R10000 Unix workstations. In the near future I will expand this list, so check the GRAMM site for the updates. Interestingly, GRAMM also works on a PC platform under Windows95 (the performance on P5-120 with 16 MB RAM is only two times slower than on SGI 250 MHZ Indigo2 R4400). Papers on GRAMM methodology * E. Katchalski-Katzir, I. Shariv, M. Eisenstein, A. A. Friesem, C. Aflalo, I. A. Vakser, 1992, Molecular surface recognition: Determination of geometric fit between proteins and their ligands by correlation techniques, Proc. Natl. Acad. Sci. USA, 89, 2195-2199. (Basic algorithm of protein recognition by correlation technique with Fast Fourier transform. High-resolution 'geometric' docking). * I. A. Vakser, C. Aflalo, 1994, Hydrophobic docking: A proposed enhancement to molecular recognition techniques, Proteins, 20, 320-329. (High-resolution 'hydrophobic' docking). * I. A. Vakser, G. V. Nikiforovich, 1995a, Protein docking in the absence of detailed molecular structures, in: Methods in Protein Structure Analysis (M. Z. Atassi & E. Appella, eds.), Plenum Press, New York, pp. 505-514. * I. A. Vakser, 1995b, Protein docking for low-resolution structures, Protein Eng., 8, 371- 377. ('Low-resolution' protein docking). * I. A. Vakser, 1996a, Long-distance potentials: An approach to the multiple-minima problem in ligand-receptor interaction, Protein Eng., 9, 37-41. (Interpretation of the low-resolution docking in terms of energy potentials). * I. A. Vakser, 1996b, Low-resolution docking: Prediction of complexes for underdetermined structures, Biopolymers, in press (39, No.3). (Validation of the low-resolution docking). * I. A. Vakser, 1996c, Main-chain complementarity in protein-protein recognition, Protein Eng., in press (9, No.7). (Docking of Ca structures). ------------------------------------------------------------------ Ilya A. Vakser The Rockefeller University, Box 270 1230 York Avenue, New York, NY 10021 Phone: (212) 327-7206; Fax: (212) 327-7540 Email: ilya@guitar.rockefeller.edu ------------------------------------------------------------------ From owner-7tms_r@net.bio.net Mon Aug 12 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in3.uu.net!EU.net!main.Germany.EU.net!fu-berlin.de!news.dfn.de!news.embl-heidelberg.de!bioftp.unibas.ch!infobiogen.fr!pasteur.fr!univ-lyon1.fr!news-rocq.inria.fr!irisa.fr!univ-rennes1.fr!univ-angers.fr!ciril.fr!usenet From: regnault@hemato.u-nancy.fr (Veronique REGNAULT) Newsgroups: bionet.molbio.proteins.7tms_r Subject: pI of streptokinase Date: 13 Aug 1996 12:19:55 GMT Organization: Hématologie, Faculté de Médecine, Nancy, France Lines: 1 Message-ID: <4uprtb$3dk@arcturus.ciril.fr> Reply-To: regnault@hemato.u-nancy.fr NNTP-Posting-Host: micro5.hemato.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: WinVN 0.92.6+ Hye, I am searching for the pI of streptokinase; Thanks very much to send the response to the above E-mail address. From owner-7tms_r@net.bio.net Mon Aug 12 23:00:00 1996 Path: biosci!QM.SERVER.UFL.EDU!jkhlab From: jkhlab@QM.SERVER.UFL.EDU ("JKHLab") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: pI of streptokinase Date: 13 Aug 1996 07:17:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Reply to: RE>pI of streptokinase Hello, The pI of the human isoform of streptokinase was shown to be 5.2 by Einarsson M et al. Biochim Biophys Acta 1979;568:19-29 and confirmed by Nowicki et al Thrombosis and Haemostasis 1994; 72:595-603. Additionally, the equine isoform was shown to have two isoforms with pI values of 5.5 and 5.7 as reported by Nowicki et al. I hope this helps. Stephen Nowicki University of Florida College of Medicine stn@icbr.ifas.ufl.edu From owner-7tms_r@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!UCDAVIS.EDU!mxkoolpe From: mxkoolpe@UCDAVIS.EDU (Mitchell Koolpe) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 14 Aug 1996 11:08:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe mxkoolpe@ucdavis.edu From owner-7tms_r@net.bio.net Mon Aug 19 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news.cse.psu.edu!news.ecn.bgu.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!EU.net!Norway.EU.net!nntp.uio.no!nntp.uib.no!nntp-bergen.UNINETT.no!nntp-trd.UNINETT.no!news.uit.no!atf1!edvard From: edvard@imb.fm.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Mutant database GRAP update Date: 20 Aug 1996 12:03:39 GMT Organization: University of Tromsoe Lines: 50 Distribution: world Message-ID: <4vc9ir$fqb@news.uit.no> Reply-To: edvard@imb.fm.uit.no NNTP-Posting-Host: atf1.imb.fm.uit.no The mutant database GRAP has been updated. In the current update there are no new mutant data added to GRAP. However, we work hard to provide mutant data in the future as well. The current update is focused on new access facilities to enhance the usefulness of the database and to exploit the existing data as much as possible. New functionality: 1) Complete list of papers referenced in the database, organized according to receptor class. 2) Complete list of ligands organized according to receptor class and agonist/antagonist classification. 3) The mutant lists (e.g. returned from a search for all substitutions in a particular receptor domain) can have two different formats for multiple point mutants. 4) All short format lists of mutants can be searched for n-fold change in ligand binding. 5) The alignment was updated to enable searching for all mutants involving substitution(s) at a particular alignment position. 6) The alignment was updated to enable searching for all mutants registered for a single receptor. Furthermore, there are certain other enhancements, changes, updates and corrections. The database has also been moved to another server. However, the address remains the same: http://www-grap.fagmed.uit.no/GRAP/homepage.html If you have problems with the new version of GRAP, you can still access the original version on http://atf1.imb.fm.uit.no/GRAP/homepage.html (at least for some time). The extended search facilities in GRAP were presented at the Nordic-Baltic symposium on Heptahelix Receptors, August 15-16, Uppsala, Sweden. -oed. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Tue Aug 20 23:00:00 1996 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Aug 1996 03:50:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!SUN.UCHC.EDU!hla From: hla@SUN.UCHC.EDU (Timothy Hla) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 22 Aug 1996 08:38:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe Timothy Hla, PhD Associate Professor Department of Physiology, MC3505 University of Connecticut School of Medicine 263 Farmington Avenue Farmington, CT 06030 ph: 860-679-4128 fx: 860-679-1269 e-mail: hla@sun.uchc.edu From owner-7tms_r@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!PHARMACOP.COM!vdfitz From: vdfitz@PHARMACOP.COM (Dan Fitzpatrick) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 28 Aug 1996 05:12:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net /\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ | Dan Fitzpatrick, PhD vdfitz@pharmacop.com | Pharmacopeia, Inc. phone: 609-452-3715 | Department of Biology fax: 609-655-4187 | 3000 East Park Blvd. | Cranbury NJ 08512 \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/ From owner-7tms_r@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!UH.EDU!Standifer From: Standifer@UH.EDU (Kelly Standifer) Newsgroups: bionet.molbio.proteins.7tms_r Subject: recombinant adenylyl cyclases Date: 28 Aug 1996 15:08:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I8TEYV4IUK00140L@Post-Office.UH.EDU> NNTP-Posting-Host: net.bio.net Hi, I've recently purchased some adenylyl cyclase antisera from Santa Cruz and was wondering if recombinant adenylyl cyclase isoforms also were commercially available for use as positive controls? I've had no success locating any. Of course, I'd gladly accept free handouts! Thanks in advance for your input, Kelly *************************************************************************** Kelly M. Standifer Assistant Professor Dept. Pharmacological and Pharmaceutical Sciences University of Houston 4800 Calhoun Houston, TX 77204-5515 USA phone: 713-743-1771 fax:713-743-1229 "Madness takes its toll.....please have exact change. From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!BAYOU.UH.EDU!sxn83991 From: sxn83991@BAYOU.UH.EDU (savitha) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 29 Aug 1996 21:06:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!BAYOU.UH.EDU!sxn83991 From: sxn83991@BAYOU.UH.EDU (savitha nagaraja) Newsgroups: bionet.molbio.proteins.7tms_r Subject: AccessUH Starting Point Date: 29 Aug 1996 20:43:48 -0700 Organization: university of houston Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I8V4ZWYW6Y00140L@Post-Office.UH.EDU> NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!mcsun!EU.net!howland.erols.net!cs.utexas.edu!swrinde!news.uh.edu!news.uth.tmc.edu!usenet From: tblevins@gsbs3.gs.uth.tmc.edu (Tracy Blevins) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Hela cells and adenylycylase Date: Thu, 29 Aug 96 15:13:33 PST Organization: The University of Texas Houston Health Science Center Lines: 13 Message-ID: <504tr9$q9p@oac2.hsc.uth.tmc.edu> NNTP-Posting-Host: farmr7.med.uth.tmc.edu Mime-Version: 1.0 X-Newsreader: WinVN 0.93.02 Hello, I am looking for information about the Hela cell line. Specifically: 1. Does it express adenylylcylase (any type)? 2. Does it express G proteins? 3. Does it express beta 2 adrenergic receptors? I would appreciate any information you might have on this topic. Thanks, Tracy Blevins From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU (Bryan Roth) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Santa Cruz antibodies Date: 29 Aug 1996 09:34:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608291635.QAA13610@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net >In response to recent postings about Santa Cruz antibodies, I wonder >if people would post their success, or lack of success, with these >reagents. > >I have heard from a number of people who have had no success with >the antibodies they obtained. Maybe an informal poll would be useful >in this forum. > >Ken Minneman We've used antibodies (Gq and Gq/G11) at both authentic recombinant Gq we prepared in the lab as well as against cell extracts. Both antibodies behaved as advertised and are routinely used. We've also used their anti-PKC subtype antibodies with good success. Caveat emptor though applies with all biological reagents--you always need to check out with authentic standards and do the appropriate controls. Bryan Roth From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Santa Cruz antibodies Date: 29 Aug 1996 08:35:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Ken Minneman wrote: >In response to recent postings about Santa Cruz antibodies, I wonder >if people would post their success, or lack of success, with these >reagents. > >I have heard from a number of people who have had no success with >the antibodies they obtained. Maybe an informal poll would be useful >in this forum. I am testing two or three of their anti-G(alpha) antisera today. I will be glad to post the results of this little experiment, and I look forward to other responses as well. As an aside, it is my understanding that Santa Cruz normally ships sera at ambient temperature, not on ice or frozen. When I send home-grown sera around, I always ship it frozen. While I am not an antibody expert, I wonder if some reports of failure with Santa Cruz sera could be due to their preferred method of shipment. AJW ------------------------------------------------------------------------ __ __ __ A. John Watson, Ph.D. /__/__/__/\ Bristol-Myers Squibb Company /__/__/__/\/\ Pharmaceutical Reseach Institute /__/__/__/\/\/\ CNS Molecular Biology, Dept. 405 \__\__\__\/\/\/ 5 Research Parkway \__\__\__\/\/ Wallingford, CT 06492 \__\__\__\/ watson_j@bms.com | 203.284.6745 voice | Standard Disclaimers Apply 203.284.7569 fax | ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!cssun.mathcs.emory.edu!news.service.emory.edu!news From: KPM Newsgroups: bionet.molbio.proteins.7tms_r Subject: Santa Cruz antibodies Date: Thu, 29 Aug 1996 09:21:36 -0700 Organization: Emory University Lines: 9 Message-ID: <3225C390.7CAB@pharm.emory.edu> NNTP-Posting-Host: minnpc.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win16; I) In response to recent postings about Santa Cruz antibodies, I wonder if people would post their success, or lack of success, with these reagents. I have heard from a number of people who have had no success with the antibodies they obtained. Maybe an informal poll would be useful in this forum. Ken Minneman From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!mcsun!EU.net!howland.erols.net!swrinde!cssun.mathcs.emory.edu!news.service.emory.edu!news From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Santa Cruz antibodies Date: Thu, 29 Aug 1996 13:02:36 -0700 Organization: Emory University Lines: 12 Message-ID: <3225F75C.10F2@bimcore.emory.edu> References: <199608291635.QAA13610@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I; 16bit) We've had very disappointing results in Westerns and immunohistochemistry with three of their anti-NFAT (anti-peptide) antibodies. These antibodies were shipped on wet ice. -- T.J. Murphy, Ph.D. Assistant Professor Department of Pharmacology 404-727-2467 Emory University School of Medicine 404-727-0365 (fax) 5031 O.W. Rollins Research Building medtjm@bimcore.emory.edu Atlanta, GA 30322 From owner-7tms_r@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!SPINE.MED.UTORONTO.CA!milton From: milton@SPINE.MED.UTORONTO.CA (Milton Charlton) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Muscarinic receptors Date: 29 Aug 1996 10:25:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Are there any cases of muscarinic Ca++ responses which can not be blocked by atropine or scopolamine? With 1nM muscarine we get intracellular Ca release seen with fluo3 which can not be blocked with 20uM atropine. O Oxotremorine also gives Ca responses. Specific nicotinic agonists and antagonists have no effect (nicotine, pancuronium, a-bungarotoxin, k-bungarotxin). Gallamine, however, does block muscarinic Ca signals. This is in frog tissue. Naturally we want to discover m6 |) but I guess there could be modifications of known receptors which could give these results. Does anyone have suggestions on how to interpret this? Thanks, Milton Charlton From owner-7tms_r@net.bio.net Thu Aug 29 23:00:00 1996 Path: biosci!PEAPLANT.BIOLOGY.YALE.EDU!staub From: staub@PEAPLANT.BIOLOGY.YALE.EDU (Jeff Staub) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 30 Aug 1996 14:36:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <32275E23.753C@peaplant.biology.yale.edu> NNTP-Posting-Host: net.bio.net subscribe -- ______________________________________________________________________ Jeffrey M. Staub Lab: (203) 432-8909 Yale University Fax: (203) 432-3854 Department of Biology http://peaplant.biology.yale.edu:8001/denglab.html OML 301 New Haven, CT 06520-8104 From owner-7tms_r@net.bio.net Thu Aug 29 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!cssun.mathcs.emory.edu!news.service.emory.edu!news From: KPM Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Hela cells and adenylycylase Date: Fri, 30 Aug 1996 10:58:44 -0700 Organization: Emory University Lines: 20 Message-ID: <32272BD4.690@pharm.emory.edu> References: <504tr9$q9p@oac2.hsc.uth.tmc.edu> NNTP-Posting-Host: minnpc.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win16; I) Tracy Blevins wrote: > > Hello, > > I am looking for information about the Hela cell line. > Specifically: > 1. Does it express adenylylcylase (any type)? > 2. Does it express G proteins? > 3. Does it express beta 2 adrenergic receptors? > > I would appreciate any information you might have on this topic. > > Thanks, Tracy Blevins They have functional beta-2 receptors linked to cAMP accumulation. Much of this data is very old. Check out PNAS 74: 873-877 (1977) by Tallman et al. The receptors can be induced with butyrate. Ken Minneman From owner-7tms_r@net.bio.net Thu Aug 29 23:00:00 1996 Path: biosci!ulb.ac.be!swillens From: swillens@ulb.ac.be (=?iso-8859-1?Q?St=E9phane?= Swillens) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 30 Aug 1996 01:03:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608300802.KAA09045@resu1.ulb.ac.be> NNTP-Posting-Host: net.bio.net subscribe St=E9phane Swillens IRIBHN - Facult=E9 de M=E9decine Universit=E9 Libre de Bruxelles fax: 32-2-5554655