From owner-7tms_r@net.bio.net Sun Jan 05 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!csulb.edu!hammer.uoregon.edu!arclight.uoregon.edu!nntp.uio.no!nntp-trd.UNINETT.no!news.uit.no!atf1!edvard From: edvard@imb.fm.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GRAP mutant database instabilities Date: 6 Jan 1997 15:03:58 GMT Organization: University of Tromsoe Lines: 25 Distribution: world Message-ID: <5ar48u$b76@news.uit.no> Reply-To: edvard@imb.fm.uit.no NNTP-Posting-Host: atf1.imb.fm.uit.no During this week we will concentrate all our Web-activities on one brand new server. This means that the GRAP databases may not work as expected or not be accessible at all during periods of this week. The reorganization is expected to be completed at the latest during friday (Jan. 10). I'll post to this newsgroup when everything is in place and (hopefully) working as expected. Wish you all a happy new year! Sincerely, OEyvind. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Mon Jan 06 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!arclight.uoregon.edu!news.ibm.net.il!news.biu.ac.il!news.huji.ac.il!usenet From: Goldblum Amiram Newsgroups: bionet.molbio.proteins.7tms_r Subject: aubscribe 7tms_r Date: Tue, 07 Jan 1997 16:00:30 +0000 Organization: The hebrew University of Jerusalem Lines: 1 Message-ID: <32D2731E.4CDF@vms.huji.ac.il> NNTP-Posting-Host: amimac.md.huji.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) subscribe 7tms_r From owner-7tms_r@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!news.uit.no!edvard From: edvard@fagmed.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GRAP mutant database instabilities may continue Date: 9 Jan 1997 14:58:37 GMT Organization: Univ. of Tromso, IMB Lines: 32 Sender: edvard@fa13.imb.fm.uit.no () Distribution: world Message-ID: <5b312t$9gm@news.uit.no> NNTP-Posting-Host: fa13.imb.fm.uit.no We have now completed the move of the GRAP databases to the new server, and done some testing and minor changes to software to get things going under the current operating system. However, tomorrow Friday 10, the university computer-center people will relocate physically a number of computers and some of the local network facilities will be closed down. That may also affect the operation of the GRAP databases and prolong the period of instabilites and inaccessibility. All our Web-documents, including the GRAP databases will now be accessible as normal, but may suffer from network down-time beyond our control. Thus, from our point of view we are back to normal operation from now on. Sincerely, Oyvind Edvardsen. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Thu Jan 09 22:00:00 1997 Path: biosci!Lilly.com!SHARP_JOHN_D From: SHARP_JOHN_D@Lilly.com ("J.D.SHARP 317-276-4268 DROP 04") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 10 Jan 1997 13:27:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <5759251610011997.A52664.MCVAX4.11B154193500*@MHS> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Thu Jan 09 22:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: Fwd: Re: would you be my friend? and Moderation. Date: 10 Jan 1997 13:04:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701102103.WAA01116@nu> NNTP-Posting-Host: net.bio.net ------- start of forwarded message (RFC 934 encapsulation) ------- Received: from net.bio.net (net.bio.net [204.31.212.2]) by stork.EMBL-Heidelberg.DE (8.7.1/8.7.1) with SMTP id VAA19035 for ; Fri, 10 Jan 1997 21:41:06 +0100 (MET) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id LAA06560; Fri, 10 Jan 1997 11:52:12 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id LAA06556; Fri, 10 Jan 1997 11:52:11 -0800 Sender: daemon@net.bio.net Message-ID: NNTP-Posting-Host: net.bio.net From: sredhar@itsa.ucsf.edu ("Sunil P. Sreedharan") To: 7tms_r@net.bio.net Subject: Re: would you be my friend? Date: 10 Jan 1997 11:52:08 -0800 >Hello, > >I'm 14 years old and I think I may be a gay. I'm looking for some support >and friendship >with a older male age 18-40. Please email if you can help. This is the last straw for me! Is there any way we can moderate this group or maintain some sort of filter that keeps 7tms-unrelated material off? While this list has been the source of some useful discussion and information, the volume of cross-posting has got out of hand these several months and I am beginning to consider unsubscribing. I have tried using Eudora's filters to delete posts that deal with pyramid schemes and how-to-annoy-everyone-by-mailing-to-all-lists, but I don't have the time to keep updating the filters every other day. Sunil Dear GPCR friends, I have sofar send a message to the postmaster of every machine from which theses inappropriate messages originated. In about 30% of all cases they responded. Every response was along the lines "Thank you for warning us, we will take action" (Which in contrast to a message from an institute administration normally means that something will really happen). In one case the account was 'stolen' and got blocked, in about 5 cases the people got denied access to the net (at least via that provider). There is a site where such culprits are listed. In several cases they are listed with name, address, social security number employer, etc. There are known cases where legal action has resulted from reporting inappropriate mails like these. The big problem is not that just we, GPCR fans, get this mail, but that there exists programs that automatically send mails to 10 thousand or more mailing lists/news groups. Moderation would help, but one needs a person who is more or less always there to do the moderation job. Part of moderation can be done by filters, but we had this discussion some years ago with this same group, and decided against it. If we are going to have this discussion again now, I suggest you mail your opinion to just me (Vriend@EMBL-Heidelberg.DE). If there are 10 or more responses, I will send them out in one big mail in 2 or 3 weeks. I will keep informing Postmasters till the number of sick-mails gets over 5 a day. When that happens I un-subscribe. Gert Vriend ------- end ------- From owner-7tms_r@net.bio.net Thu Jan 09 22:00:00 1997 Path: biosci!ITSA.UCSF.EDU!sredhar From: sredhar@ITSA.UCSF.EDU ("Sunil P. Sreedharan") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: would you be my friend? Date: 10 Jan 1997 11:52:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5b4ti5$kmr@news1-alterdial.uu.net> NNTP-Posting-Host: net.bio.net >Hello, > >I'm 14 years old and I think I may be a gay. I'm looking for some support >and friendship >with a older male age 18-40. Please email if you can help. This is the last straw for me! Is there any way we can moderate this group or maintain some sort of filter that keeps 7tms-unrelated material off? While this list has been the source of some useful discussion and information, the volume of cross-posting has got out of hand these several months and I am beginning to consider unsubscribing. I have tried using Eudora's filters to delete posts that deal with pyramid schemes and how-to-annoy-everyone-by-mailing-to-all-lists, but I don't have the time to keep updating the filters every other day. Sunil PS. While on the topic of 7tms, what is the status of the CGRP receptor these days? There are two groups that have published unrelated sequences for this receptor. One of them is related to the adrenomedulin receptor, and to the orphan receptor RDC1 with which I've had a brush with. I was not able to detect CGRP binding or signal transduction using the human homolog of RDC1 (GPRN1). Has anyone had better luck in verifying these findings? Sunil P. Sreedharan, Ph.D. Assistant Professor Lab. of Allergy & Immunol. Res. Dept. of Medicine, UCSF 432A Irving, Box 0954 San Francisco, CA 94143 Tel. (415) 476-9895 Fax (415) 502-4175/(415) 476-9895 email sredhar@itsa.ucsf.edu From owner-7tms_r@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Jan 1997 02:00:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701121000.CAA21604@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms: supplementary bridges in chemokine receptors ? Date: 12 Jan 1997 23:55:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, Does anybody know experimental facts concerning supplementary disulfide bridges in the chemokine (alpha or beta) receptors which belong to GPCR superfamily? Any hints or references would be grately appreciated. Many thanks in advance for your help. Best wishes, Gennady ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54506 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!agate!howland.erols.net!swrinde!news.uh.edu!news.uth.tmc.edu!bmb155.med.uth.tmc.edu!user From: tliang@utmmg.med.uth.tmc.edu (T. Chyau Liang) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Histone mRNA Date: 14 Jan 1997 17:46:42 GMT Organization: U. Texas-Houston Lines: 18 Message-ID: References: <5bgbdm$shd@mail.boku.ac.at> NNTP-Posting-Host: bmb155.med.uth.tmc.edu Mime-Version: 1.0 Content-Type: text/plain;charset=US-ASCII Content-Transfer-Encoding: 7bit In article <5bgbdm$shd@mail.boku.ac.at>, iam@mail.boku.ac.at (Georg Seifert) wrote: > Hi > Is Histone (H4) mRNA polyadenylated? (If not, Ref´s please) > > Histone mRNAs don't go throught the normal "maturation" processes (i.e., no splicing and polyA addition). They are transported out of nucleus by a different mechainsim. see Stryer's Biochemistry, 4th Ed., p 994. (not a primary reference, though). Hope this helps. T. Chyau Liang U. Texas-Houston From owner-7tms_r@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!arclight.uoregon.edu!hammer.uoregon.edu!news.icm.edu.pl!uw.edu.pl!news.nask.pl!01-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!mail.boku.ac.at!news From: iam@mail.boku.ac.at (Georg Seifert) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Histone mRNA Date: 14 Jan 1997 16:13:45 GMT Organization: IAM Institute of Applied Microbiology Lines: 4 Message-ID: <5bgbbp$shd@mail.boku.ac.at> NNTP-Posting-Host: 141.244.135.61 X-Newsreader: WinVN version 0.82 Hi Is Histone (H4) mRNA polyadenylated? (If not, Ref´s please) Cheese Georg From owner-7tms_r@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!agate!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!su-news-hub1.bbnplanet.com!arclight.uoregon.edu!hammer.uoregon.edu!news.icm.edu.pl!uw.edu.pl!news.nask.pl!01-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!mail.boku.ac.at!news From: iam@mail.boku.ac.at (Georg Seifert) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Histone mRNA Date: 14 Jan 1997 16:14:46 GMT Organization: IAM Institute of Applied Microbiology Lines: 6 Message-ID: <5bgbdm$shd@mail.boku.ac.at> NNTP-Posting-Host: 141.244.135.61 X-Newsreader: WinVN version 0.82 Hi Is Histone (H4) mRNA polyadenylated? (If not, Ref´s please) Cheese Georg From owner-7tms_r@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!BMB.LEEDS.AC.UK!bmb6hdd From: bmb6hdd@BMB.LEEDS.AC.UK Newsgroups: bionet.molbio.proteins.7tms_r Subject: 2nd-messenger-depend-kinase desensitisation of Gi-linked GPCRs Date: 16 Jan 1997 01:44:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <009AE71A.3017D6E5.25@bmb.leeds.ac.uk> NNTP-Posting-Host: net.bio.net From: MX%"bmbnmh@south-01.novell.leeds.ac.uk" 25-NOV-1996 08:30:03.38 To: MX%"lifenet@central.admin.leeds.ac.uk" CC: Subj: Seminar Return-Path: Received: from registry.leeds.ac.uk by BMBAXP.leeds.ac.uk (MX V4.2 AXP) with SMTP; Mon, 25 Nov 1996 08:29:56 GMT Received: from central.admin.leeds.ac.uk (central0.leeds.ac.uk [129.11.232.150]) by registry.leeds.ac.uk (8.6.12/8.6.12) with ESMTP id IAA24253; Mon, 25 Nov 1996 08:31:27 GMT Received: from CENTRAL/MAILQUEUE by central.admin.leeds.ac.uk (Mercury 1.21); 25 Nov 96 08:26:35 +0 Received: from MAILQUEUE by CENTRAL (Mercury 1.21); 25 Nov 96 08:26:04 +0 From: "N.M. HOOPER" To: "Lifesciences and Healthcare Network" Subject: Seminar Date: Mon, 25 Nov 1996 08:30:39 GMT0BST Sender: Maiser@central.admin.leeds.ac.uk X-listname: Organization: University of Leeds X-mailer: Pegasus Mail for Windows (v2.42a) (via Mercury MTS v1.21) Message-ID: <107EA566C9F@central.admin.leeds.ac.uk> Department of Biochemistry and Molecular Biology Seminar on Wednesday 27th November at 1.00pm in the Dental Lecture theatre, Level 6, Worsley Building Dr Colin Stirling Department of Biochemistry and Molecular Biology, University of Manchester 'Molecular chaperones: why proteins can't leave home without one' Host: Dr Alison Baker, x3140 ------------------- Dr Nigel M. Hooper Department of Biochemistry and Molecular Biology The University of Leeds Leeds LS2 9JT United Kingdom Tel. +44 113 233 3163 Fax. +44 113 233 3167 From owner-7tms_r@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!BMB.LEEDS.AC.UK!bmb6hdd From: bmb6hdd@BMB.LEEDS.AC.UK Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gi-linked desensitisation (sorry about garbage in my last post - wrong file sent) Date: 16 Jan 1997 03:40:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <009AE72A.4D7E2260.39@bmb.leeds.ac.uk> NNTP-Posting-Host: net.bio.net I wonder whether anyone can help me with a possibly naive question regarding 2nd-messenger-dependent desensitisation of Gi-linked receptors. Low doses of agonist result in b2-AR desensitisation via PKA rather than via GRKs and one can envisage a similar mechanism for PLC-linked receptors via PKC. However, is there any evidence for the converse occurring in Gi-coupled receptors? That is, can receptors be pre- phosphorylated (tonically) by PKA and hence protected from desensitisation until the PKA levels are reduced following receptor activation. Or is it just GRK regulated? Best Regards, Dan **************** Dr. Dan Donnelly Lecturer, Department Pharmacology University of Leeds, Leeds LS2 9JT, UK d.donnelly@leeds.ac.uk **************** From owner-7tms_r@net.bio.net Sat Jan 18 22:00:00 1997 Path: biosci!agate!howland.erols.net!torn!nott!cunews!wabakimi!nbatada From: nbatada@chat.carleton.ca (Nizar Batada) Newsgroups: bionet.molbio.proteins.7tms_r Subject: is the sequence for mdm2 known Date: 19 Jan 1997 23:41:13 GMT Organization: Carleton University, Ottawa, Canada Lines: 15 Message-ID: <5bubep$j4a@bertrand.ccs.carleton.ca> NNTP-Posting-Host: wabakimi.carleton.ca NNTP-Posting-User: nbatada X-Newsreader: TIN [version 1.2 PL2] Hi, i am trying to find the protein sequence for MDM2 (p53 binding protein). I tried the Entrez PDB and could only find the data for the 101 a.a of the N-terminus regions. Could someone please tell if the whole MDM2 has been sequenced and if yes where can i find the sequnce. thanks in advance nizar ---------------------------------------------------------------------- Nizar N. Batada Carleton University, Biochemistry email address: nbatada@chat.carleton.ca ---------------------------------------------------------------------- From owner-7tms_r@net.bio.net Thu Jan 23 22:00:00 1997 Path: biosci!BCMP.MED.HARVARD.EDU!lustig From: lustig@BCMP.MED.HARVARD.EDU (Kevin Lustig) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 24 Jan 1997 15:21:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Has it been possible to construct a dominant negative mutant of any GPCR? References would be appreciated. Thanks, Kevin Kevin Lustig phone: 617-432-2294 Dept. of Cell Biology fax: 617-432-1144 Harvard Medical School e-mail: lustig@bcmp.med.harvard.edu Boston, MA 02115 From owner-7tms_r@net.bio.net Thu Jan 23 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!worldnet.att.net!news.maxwell.syr.edu!news.bc.net!rover.ucs.ualberta.ca!gallin2.biology.ualberta.ca!wgallin From: wgallin@gpu.srv.ualberta.ca (Warren Gallin) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: Re: Dot blot- not? Date: Fri, 24 Jan 97 23:46:33 GMT Organization: University of Alberta Lines: 28 Message-ID: References: <5cb20p$3ut$1@mhadf.production.compuserve.com> <32E95FB5.41C6@vax.cs.hscsyr.edu> NNTP-Posting-Host: gallin2.biology.ualberta.ca X-Newsreader: VersaTerm Link v1.1.4 Xref: biosci bionet.molbio.methds-reagnts:53923 bionet.molbio.proteins:9830 bionet.molbio.proteins.7tms_r:1018 In Article <32E95FB5.41C6@vax.cs.hscsyr.edu>, Tom Duncan wrote: >DSF wrote: >> >> Is there a way to do protein dot blots with denatured protein >> (that stays denatured on the membrane)? Got a protocol? >> Thanks >> Lynette > >Of course, you can't apply most denatured proteins in the presence of >SDS, since the SDS drastically reduces the binding of most proteins to >blotting membranes (nitrocellulose, PVDF). Virtually all Western blotting is done on SDS-denatured protein using an SDS-containing buffer, and the results can be both sensitive and quantitative, so I wouldn't dismiss simple SDS denaturation and dotting out of hand. It would certainly be worth a try. As far as staying denatured on the membrane, I would guess that the proportion of a protein that remains denatured under given blotting conditions will be variable, depending on both the nature of the blotting conditions and the identity of the protein; once again, I think you have to be empirical. Warren Gallin Department of Biological Sciences University of Alberta Edmonton, Alberta T6G 2E9 Canada wgallin@gpu.srv.ualberta.ca From owner-7tms_r@net.bio.net Thu Jan 23 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!howland.erols.net!newsxfer3.itd.umich.edu!newsfeed.internetmci.com!compuserve.com!news.production.compuserve.com!news From: DSF <76042.532@CompuServe.COM> Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: Dot blot- not? Date: 24 Jan 1997 19:19:53 GMT Organization: Cadus Lines: 4 Message-ID: <5cb20p$3ut$1@mhadf.production.compuserve.com> Xref: biosci bionet.molbio.methds-reagnts:53912 bionet.molbio.proteins:9824 bionet.molbio.proteins.7tms_r:1015 Is there a way to do protein dot blots with denatured protein (that stays denatured on the membrane)? Got a protocol? Thanks Lynette From owner-7tms_r@net.bio.net Thu Jan 23 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!news-atl-21.sprintlink.net!news.hscsyr.edu!newsadm From: Tom Duncan Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: Re: Dot blot- not? Date: Fri, 24 Jan 1997 17:19:49 -0800 Organization: SUNY Health Science Ctr, Dept Biochemistry Lines: 42 Message-ID: <32E95FB5.41C6@vax.cs.hscsyr.edu> References: <5cb20p$3ut$1@mhadf.production.compuserve.com> NNTP-Posting-Host: cross.bmb.hscsyr.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0S (X11; I; IRIX 6.2 IP22) Xref: biosci bionet.molbio.methds-reagnts:53920 bionet.molbio.proteins:9829 bionet.molbio.proteins.7tms_r:1016 DSF wrote: > > Is there a way to do protein dot blots with denatured protein > (that stays denatured on the membrane)? Got a protocol? > Thanks > Lynette Of course, you can't apply most denatured proteins in the presence of SDS, since the SDS drastically reduces the binding of most proteins to blotting membranes (nitrocellulose, PVDF). You may be able to use heat denaturation before applying the protein to the blot, as long as your protein does not refold too quickly as it cools (before or after spotting on blot). Another possibility is denaturating with urea, and you may be able to dilute the urea several fold before blotting as long as the protein(s) you're interested in do not refold too rapidly as the urea is diluted. You would also have to test how well your protein(s) binds to the blotting membrane in the presence of urea. As you noted, a further problem is that, after dot-blotting, some denatured proteins can refold during incubation of the blot in the common buffers used for incubation with blocking agents and antibodies. Although I did not test this for different proteins at the time, one approach that worked in a specific case involved blotting on a nitrocellulose membrane and then baking the blot at 80deg (in a vacuum oven) to denature the protein and covalently link it to the nitrocellulose to "lock" it in the denatured state. Subsequently, some MAbs that recognized the protein on western blots also detected it on the dot blot, but another MAb could not detect the protein on westerns or on the denatured dot blot, and so probably recognizes a "conformational" epitope. The reference: Adami et al. (1993) Biochem. J. 292, 863-872. Good luck, and let me know if you are successful. Tom -- Thomas M. Duncan Dept. Biochemistry & Molecular Biology SUNY Health Science Center 750 E Adams St, Syracuse, NY 13210 Email: duncant@vax.cs.hscsyr.edu From owner-7tms_r@net.bio.net Sun Jan 26 22:00:00 1997 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news-xfer.netaxs.com!feed1.news.erols.com!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!news-pen-15.sprintlink.net!news.hscsyr.edu!newsadm From: Tom Duncan Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: Re: Dot blot- not? Date: Mon, 27 Jan 1997 10:55:45 -0800 Organization: SUNY Health Science Ctr, Dept Biochemistry Lines: 35 Message-ID: <32ECFA31.167E@vax.cs.hscsyr.edu> References: <5cb20p$3ut$1@mhadf.production.compuserve.com> <32E95FB5.41C6@vax.cs.hscsyr.edu> NNTP-Posting-Host: cross.bmb.hscsyr.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0S (X11; I; IRIX 6.2 IP22) Xref: biosci bionet.molbio.methds-reagnts:54000 bionet.molbio.proteins:9847 bionet.molbio.proteins.7tms_r:1019 Warren Gallin wrote: > > In Article <32E95FB5.41C6@vax.cs.hscsyr.edu>, Tom Duncan > wrote: > >Of course, you can't apply most denatured proteins in the presence of > >SDS, since the SDS drastically reduces the binding of most proteins to > >blotting membranes (nitrocellulose, PVDF). > > Virtually all Western blotting is done on SDS-denatured protein using an > SDS-containing buffer, and the results can be both sensitive and > quantitative, so I wouldn't dismiss simple SDS denaturation and dotting out > of hand. It would certainly be worth a try. For western transfer, the %SDS is usually reduced several fold from the 0.1% used in Laemmli gel-running buffer; the higher %SDS increases the rate of transfer of most proteins, but also reduces the binding of many proteins to transfer membranes (although PVDF seems less affected than nitrocellulose). Also, even 0.1% SDS is not enough to completely denature some proteins. > As far as staying denatured on the membrane, I would guess that the > proportion of a protein that remains denatured under given blotting > conditions will be variable, depending on both the nature of the blotting > conditions and the identity of the protein; once again, I think you have to > be empirical. Part of my original suggestion was that the use of nitrocellulose and baking the blot could be used to covalently fix the protein in the denatured state on the blot. Cheers, -- Thomas M. Duncan Dept. Biochemistry & Molecular Biology SUNY Health Science Center 750 E Adams St, Syracuse, NY 13210 Email: duncant@vax.cs.hscsyr.edu From owner-7tms_r@net.bio.net Tue Jan 28 22:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!newsfeeds.sol.net!newspump.sol.net!howland.erols.net!rill.news.pipex.net!pipex!netcom.net.uk!xara.net!emerald.xara.net!news.thenet.net!uunet!in2.uu.net!194.179.1.100!minerva.ibernet.es!noticias.adv.es!jbb@adv.es From: "Federico Velasco" Newsgroups: bionet.molbio.proteins.7tms_r Subject: register Date: 30 Jan 1997 00:05:44 GMT Organization: Layout, S. L. Lines: 1 Message-ID: <01bc0ca2$e87a5da0$c3cfe0c2@rams-s> NNTP-Posting-Host: 194.224.207.174 X-Newsreader: Microsoft Internet News 4.70.1155 I want some information about register. Thank you. From owner-7tms_r@net.bio.net Thu Jan 30 22:00:00 1997 Path: biosci!agate!howland.erols.net!rill.news.pipex.net!pipex!news.sprintlink.net!news-peer.sprintlink.net!cs.utexas.edu!geraldo.cc.utexas.edu!netnews.uthscsa.edu!kolakowski.uthscsa.edu!kolakowski From: kolakowski@uthscsa.edu (Lee F. (Frank) Kolakowski, Jr.) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral Position(s) Followup-To: bionet.molbio.proteins.7tms_r Date: Thu, 30 Jan 97 00:51:19 GMT Organization: Department of Pharmacology - UTHSCSA Lines: 68 Message-ID: NNTP-Posting-Host: kolakowski.uthscsa.edu X-Newsreader: VersaTerm Link v1.1.6 Postdoctoral Positions University of Texas Health Science Center at San Antonio Postdoctoral positions are available to: 1) clone and characterize novel G protein-coupled receptors and their ligands and 2) generate targeting constructs for some of these novel receptors. Basic molecular biology, protein structure/function, and cell culture experience is desired. Candidates must have received a Ph.D. and/or M.D. within the last three years. Send curriculum vitae, a brief description of research experience, and the telephone numbers and addresses of three references to: Lee F. Kolakowski, Jr., Ph.D. Department of Pharmacology University of Texas Health Science Center at San Antonio 7703 Floyd Curl Dr. San Antonio, TX 78284-7763 Tel. 210-567-4233 FAX 210-567-4303 Email: Kolakowski@UTHSCSA.Edu The Kolakowski Lab The Kolakowski Lab () is a young department, with fifteen faculty developing vigorous research programs in molecular pharmacology, neuroscience, behavioral pharmacology and cardiovascular pharmacology. San Antonio An energetic city with a population over 1 million, San Antonio offers young adults and families a relaxing pace, European flavor, the world-famous Riverwalk and many other local attractions in an extremely affordable setting. Our weather is beautful for nine months of the year -- yes, it's very hot for three months, but you won't need a snow shovel! If you enjoy a blend of cultures and the best Mexican food this side of the Rio Grande, San Antonio may be the city for you. UTHSCSA is an Equal Employment Opportunity/Affirmative Action Employer Lee F. (Frank) Kolakowski, Jr. Assistant Professor Department of Pharmacology Univ. of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, TX 78284-7764 210-567-4233 Office 210-567-4303 Fax Kolakowski@uthscsa.edu or lfk@receptor.mgh.harvard.edu see also http://kolakowski.uthscsa.edu/ and http://receptor.mgh.harvard.edu/GCRDBHOME.html