From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!EXT.MISSOURI.EDU!David_L._Guarraia_at_MU-HSC-PHARM-PO1 From: David_L._Guarraia_at_MU-HSC-PHARM-PO1@EXT.MISSOURI.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 2 Apr 1997 07:09:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9703028599.AA859999813@ext.missouri.edu> NNTP-Posting-Host: net.bio.net Subscribe From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms_r: HIV and opiates Date: 2 Apr 1997 02:00:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, It is known that morphine and other narcotics alter the function of human T lymphocytes and monocytes and may lead to activation of HIV infection (ex., Squinto SP, et al. AIDS Res Hum Retroviruses, 6(10):1163-1168, 1990). Moreover, potentiation of HIV growth by morphine was antagonized by naloxone and beta-funaltrexamine (opioid antagonists) (Peterson PK, et al. AIDS 4(9):869-873, 1990). This means that opiate systems involved somehow in HIV pathogenesis. Did anyone investigate possible mechanisms? One of the possibilities can be direct binding of opiates to chemokine receptors (found to be cofactors for HIV and host cell membrane fusion). So the question, has anyone seen (or measured) binding constants of opiate ligands (antagonists or agonists) to the chemokine receptors such as CXCR-4 and CCR-5, etc.? This binding may be rather non specific but even so... Any hints or references would be greatly appreciated. I will summarize the answers. Best wishes and many thanks for your participation, Gennady ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!cochin.inserm.fr!petit From: petit@cochin.inserm.fr (Laurence Petit) Newsgroups: bionet.molbio.proteins.7tms_r Subject: SUBSCRIBE 7TMS Date: 2 Apr 1997 01:31:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net SUBSCRIBE 7TMS ____________________________________________________________________________ Jerome GROS ICGM - UPR0415 CNRS email : gros@cochin.inserm.fr *********** 22, rue Mechain Tel (33) 01 40 51 64 22/21 * BioDocs * F-75014 PARIS fax (33) 01 40 51 72 10 *********** Serveur de BioDocs : http://157.136.20.60 ____________________________________________________________________________ From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!news-paris.gsl.net!news.gsl.net!rain.fr!pressimage!news1.isdnet.net!usenet From: "Christophe Guenin" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Antibody and G-Protein Date: 2 Apr 1997 20:15:16 GMT Lines: 12 Message-ID: <01bc3faa$8683b520$99b695c2@default> NNTP-Posting-Host: ppp14.rai.hol.fr X-Newsreader: Microsoft Internet News 4.70.1155 I am looking for company which distribute antibody against the following subunits : G(alpha)q, G(alpha)11, G(alpha)14, G(alpha)16, Gh Reply on this news group or send a mail to christophe.guenin@hol.fr Thanks Sophie Lechevallier - SERVIER Labs. From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!PEAPLANT.BIOLOGY.YALE.EDU!staub From: staub@PEAPLANT.BIOLOGY.YALE.EDU (Jeff Staub) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 2 Apr 1997 11:01:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: non-internalizing receptors Date: 2 Apr 1997 08:50:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704021943.TAA03584@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net The following receptors were reported to *not* internalize following agonist exposure: alpha2-adrenergic subtype (Kobilka lab) somatostatin receptor subtype (J. Cell Biology 102: 878, 1986 JBC 261: 3571, 1986) AT2 angiontensin II receptor Someone mentioned that an antagonist causes internalization of the At1 receptor and asked about other examples of antagonist-induced internalization. We have demonstrated (Mol Pharmacol, 1996) that ketanserin (a 5-HT2A antagonist) causes modest internalization of the 5-HT2A receptor and have a manuscript in preparation demonstrating the same thing for clozapine (which also functions as an antagonist) From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 2 Apr 1997 15:06:45 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net christophe.guenin@hol.fr (Christophe Guenin) wrote: >I am looking for company which distribute antibody against the following >subunits : > G(alpha)q, > G(alpha)11, > G(alpha)14, > G(alpha)16, > Gh Let's see... Antisera against G(q) and G(11) you can get from Santa Cruz Biotechnol (http://www.scbt.com/) or DuPont NEN (http://www.nenlifesci.com/). I haven't seen or heard of anyone selling G(14) or G(h) antisera. Commercial applications of G(16), including (as far as I know) antisera, has been licensed to Aurora Biosciences (http://www.aurorabio.com/). For noncommercial use of G(16), you might try Mel Simon's lab at Caltech (http://www.caltech.edu/). Cheers, AJW From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!UMDNJ.EDU!pikielcl From: pikielcl@UMDNJ.EDU ("Claudio Pikielny") Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-doc Date: 2 Apr 1997 13:28:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Post-doctoral position available. We study Chemical Senses (olfaction, taste and pheromone response) in Drosophila at the genetic, biochemical, cellular and behavioral levels. Send CV and letters of recommendation to Claudio Pikielny, Dept. of Neuroscience and Cell Biology, UMDNJ/Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway NJ 08540. e-mail: pikielcl@umdnj.edu From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!SHOWME.MISSOURI.EDU!c680296 From: c680296@SHOWME.MISSOURI.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 2 Apr 1997 07:32:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net SUBSCRIBE David M. Bourdon Graduate Research Assistant MU School of Medicine Department of Pharmacology tel: 573-882-1554 e-mail: c680296@showme.missouri.edu home page: http://www.missouri.edu/~c680296/index.htm From owner-7tms_r@net.bio.net Tue Apr 01 23:00:00 1997 Path: biosci!MED.UNR.EDU!buxton From: buxton@MED.UNR.EDU ("Iain L. O. Buxton") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7tms_r: HIV and opiates Date: 2 Apr 1997 08:02:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Dear Dr. Poda: I am intrigued with the question you pose. Faced with a not so disimilar issue with respect to muscarinic systems and NO synthase inhibitors, we show that a not-so-high-affinity interaction can be an issue (Buxton, I.L.O., Cheek, D.J., Eckman, D.M., Westfall, D.P. Sanders, K.M. and Keef, K.D. (1993) NG-nitro-L-arginine methylester (L-NAME) and other alkyl esters of arginine are muscarinic receptor antagonists. Circ. Res. 72: 387-395.) We could look at this with you if you are interested? Iain L. O. Buxton Professor of Pharmacology/318 Associate Dean for Research University of Nevada School of Medicine Howard Medical Sciences Building Rm:216 Reno, NV 89557 Phone: (702)784-1566 FAX: (702)784-1620 buxton@med.unr.edu On 2 Apr 1997, Gennady PODA wrote: > Dear Netters, > > It is known that morphine and other narcotics alter the function of human > T lymphocytes and monocytes and may lead to activation of HIV infection > (ex., Squinto SP, et al. AIDS Res Hum Retroviruses, 6(10):1163-1168, > 1990). Moreover, potentiation of HIV growth by morphine was antagonized by > naloxone and beta-funaltrexamine (opioid antagonists) (Peterson PK, et al. > AIDS 4(9):869-873, 1990). This means that opiate systems involved somehow > in HIV pathogenesis. Did anyone investigate possible mechanisms? > > One of the possibilities can be direct binding of opiates to chemokine > receptors (found to be cofactors for HIV and host cell membrane fusion). > So the question, has anyone seen (or measured) binding constants of opiate > ligands (antagonists or agonists) to the chemokine receptors such as > CXCR-4 and CCR-5, etc.? This binding may be rather non specific but even > so... > > Any hints or references would be greatly appreciated. I will summarize > the answers. > > Best wishes and many thanks for your participation, > > Gennady > > > > > ************************************************************ > Dr. Gennady PODA > > Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) > Universite Henri Poincare, Nancy-I > Faculte des Sciences - Domaine scientifique Victor Grignard > B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France > > Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr > ************************************************************ > > From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-xfer.netaxs.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!news-paris.gsl.net!news.gsl.net!rain.fr!pressimage!news1.isdnet.net!usenet From: "Christophe Guenin" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Receptors and G_Protein Date: 3 Apr 1997 19:19:40 GMT Lines: 11 Message-ID: <01bc406c$272df460$adb695c2@default> NNTP-Posting-Host: ppp34.rai.hol.fr X-Newsreader: Microsoft Internet News 4.70.1155 Dear netters I am looking for structures of the genes of the receptors coupled to G Proteins You can answer in this news group ou send a mail to christophe.guenin@hol.fr Best wishes and many thanks for your participation Sophie-Penelope Lechevallier - SERVIER Labs From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!cochin.inserm.fr!laurence.petit From: laurence.petit@cochin.inserm.fr (Laurence Petit) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe 7tms_r Date: 3 Apr 1997 08:14:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704031603.SAA25630@zeus.cochin.inserm.fr> NNTP-Posting-Host: net.bio.net subscribe 7tms_r Laurence Petit UPR 415 Institut Cochin de genetique Moleculaire 22 rue Mechain 75014 Paris Phone : (33-1) 40-51-64-39 Fax : (33-1) 40-51-72-10 e mail : laurence.petit@cochin.inserm.fr From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeed.nacamar.de!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-feed2.bbnplanet.com!sol.caps.maine.edu!discovery.umeres.maine.edu!Keith_Hutchison From: Keith_Hutchison@discovery.umeres.maine.edu (Keith Hutchison) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Faculty Position - Protein Biochemist Date: Thu, 3 Apr 1997 08:58:23 -0500 Organization: University of Maine Lines: 18 Sender: Keith_Hutchison@discovery.umeres.maine.edu Message-ID: NNTP-Posting-Host: discovery.umeres.maine.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.18 for FirstClass(R) PROTEIN BIOCHEMIST: The Department of Biochemistry, Microbiology and Molecular Biology at the University of Maine seeks a biochemist with research interests in protein-protein or protein-nucleic acid interactions to fill a tenure track position at either the Assistant or Associate Professor level, available September 1, 1997. Additional research experience in the molecular genetics or developmental biology of aquatic organisms is desirable. A Ph.D. and postdoctoral experience are required. The successful candidate will be expected to maintain a vigorous, extramurally funded research program and contribute to teaching of the undergraduate and graduate curricula in Biochemistry. Minimum salary is $40,000 per academic year. Send a cover letter, description of research interests, curriculum vitae and three letters of recommendation to: Dr. Michael E. Vayda, Chair, Biochemistry Search Committee, 5735 Hitchner-BMMB, University of Maine, Orono, ME 04469-5735. Review of applications will commence June 1, 1997 and applications will be considered until a suitable candidate is located. Women and minorities are encouraged to apply. The University of Maine is an Affirmative Action/Equal Employment Opportunity Employer. From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!agate!howland.erols.net!netnews.com!mr.net!newshub.tc.umn.edu!mayonews.mayo.edu!not-for-mail From: Ram Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: Thu, 03 Apr 1997 17:39:18 -0600 Organization: Mayo Foundation Lines: 5 Message-ID: <33443FA6.606@mayo.edu> References: <199704021943.TAA03584@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: millerl.mayo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) To: roth@BIOCSERVER.BIOC.CWRU.EDU We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news-peer.gsl.net!mr.net!newshub.tc.umn.edu!mayonews.mayo.edu!not-for-mail From: Ram Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: Thu, 03 Apr 1997 17:40:41 -0600 Organization: Mayo Foundation Lines: 5 Message-ID: <33443FF9.70C6@mayo.edu> References: <199704021943.TAA03584@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: millerl.mayo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) To: roth@BIOCSERVER.BIOC.CWRU.EDU We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.idt.net!mr.net!newshub.tc.umn.edu!mayonews.mayo.edu!not-for-mail From: Ram Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: Thu, 03 Apr 1997 17:40:12 -0600 Organization: Mayo Foundation Lines: 5 Message-ID: <33443FDC.2773@mayo.edu> References: <199704021943.TAA03584@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: millerl.mayo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) To: roth@BIOCSERVER.BIOC.CWRU.EDU We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 From owner-7tms_r@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!agate!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!mr.net!newshub.tc.umn.edu!mayonews.mayo.edu!not-for-mail From: Ram Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: Thu, 03 Apr 1997 17:39:43 -0600 Organization: Mayo Foundation Lines: 5 Message-ID: <33443FBF.186F@mayo.edu> References: <199704021943.TAA03584@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: millerl.mayo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) To: roth@BIOCSERVER.BIOC.CWRU.EDU We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 4 Apr 1997 17:11:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHBJBQAIFS002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID z1VhsuqGtjtR1QQcBHpeaA) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID z1VhsuqGtjtR1QQcBHpeaA) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA3OM5BLS002YUV@mc.duke.edu>; Thu, 03 Apr 1997 19:36:46 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA04058; Thu, 03 Apr 1997 19:33:45 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27954; Thu, 03 Apr 1997 15:49:29 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27950; Thu, 03 Apr 1997 15:49:28 -0800 Date: Thu, 03 Apr 1997 17:39:43 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FBF.186F@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID z1VhsuqGtjtR1QQcBHpeaA)-- From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!UMDNJ.EDU!howells From: howells@UMDNJ.EDU ("Richard D. Howells, Ph.D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral fellowship Date: 4 Apr 1997 11:02:33 -0800 Organization: UMD-New Jersey Medical School Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <334543F9.AC2@umdnj.edu> Reply-To: howells@UMDNJ.EDU NNTP-Posting-Host: net.bio.net A postdoctoral fellowship will soon become available for molecular analysis of opioid receptor structure and function. Site-directed mutagenesis and receptor chimeras are being used to study receptor selectivity, activation, signal transduction coupling, and turnover (cf. Wang et al., PNAS 92: 12436-12440, 1995; Shahrestanifar et al., J. Biol. Chem. 271: 5505-5512, 1996; Neurochem. Res. 21: 1295-1299, 1996). Please send CVs to Dr. Richard D. Howells, Department of Biochemistry & Molecular Biology, UMD-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103. Richard D. Howells, Ph.D. Associate Professor From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 4 Apr 1997 16:58:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHBIVBJOQ0002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID CkLzsp+aZeRNRq1xdAKc4w) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID CkLzsp+aZeRNRq1xdAKc4w) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA39ZWA00000LKM@mc.duke.edu>; Thu, 03 Apr 1997 19:25:00 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03837; Thu, 03 Apr 1997 19:21:42 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28525; Thu, 03 Apr 1997 15:53:21 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28520; Thu, 03 Apr 1997 15:53:19 -0800 Date: Thu, 03 Apr 1997 17:40:12 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FDC.2773@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID CkLzsp+aZeRNRq1xdAKc4w)-- From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: jpwscyto@pavilion.co.uk (Jon Waterman-Smith) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Forthcoming Microphysiometry Symposium in Paris Date: 4 Apr 1997 17:06:09 +0100 Lines: 249 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5i38th$e2f@mserv1.dl.ac.uk> X-Sender: jpwscyto@mailhost.pavilion.co.uk Original-To: 7tms_r@dl.ac.uk Please find enclosed details regarding this forthcoming meeting in Paris. If you wish to receive further information on this event, please call Rana =46riend at MDL [+44 (0)1293 619579], and we will send you full registration information. The 1997 International Symposium on Microphysiometry and Fluorescent Cell Analysis. Paris, France - April 28-30, 1997. Paris in spring time... We hope this will be a suitable location for the first International Symposium on Microphysiometry and FLIPR. Venue: The Hyatt Regency Paris Hotel, Charles de Gaulle, Paris. The Hyatt is a superior hotel on the outskirts of Paris, 5 minutes from Charles de Gaulle airport, and only 20 minutes from the heart of Paris, with direct rail links from Charles de Gaulle. Dates: Monday 28th to Wednesday 30th April 1997. Registration: 5:00 p.m. to 7:00 p.m. on Sunday 27th and 8:00 a.m. to 9:00 a.m. on Monday 28th of April. Travel: Many airlines provide direct International links to Paris, Charles de Gaulle. The Hyatt provides a courtesy bus to the hotel from the airport. Accomodation and registration costs: We have made a block booking at the hotel for the period of this conference. The price for the rooms and registration have been fixed as follows. If you wish to book rooms for the Saturday evening as well, accomodation will be available: Sunday 27th April - 950FF ( =A3110 or $180 ) room only. Monday 28th April - 950FF ( =A3110 or $180 ) room only. Tuesday 29th April - 950FF ( =A3110 or $180 ) room only. A one-off registration fee of 850FF ( =A3100 or $165 ). Molecular Devices is directly funding the majority of the costs associated with this conference, but we need to charge a small registration fee to assist in contributing to the cost of this event. All meals will be provided on the Monday, including a gala dinner. A presentation for the most original contribution to research presented at this Symposium will be given. All meals (except evening meals) on the Tuesday and Wednesday are also covered in the registration fee. A pre-registration form will be distributed to all members of the email list in the coming weeks, and we would ask that you return this as soon as possible if you wish to register, to ensure accomodation is reserved, as we are limited to the number of guarenteed rooms. =46inal Program: Intl Symposium on Microphysiometry & Fluorometric Cellular Screening Monday Morning, April 28, 1997 Opening Remarks 8:40-8:50 Chairperson, Dr. Simon Pitchford, Applications Manager, Molecular Devices, USA G Protein-Coupled Receptors Martyn Coldwell 8:50-9:30 Smith-Kline Beecham, UK martyn_coldwell-1@sbphrd.com Evolution of microphysiometer screening methods for increased throughput functional studies on cloned dopaminergic receptors. M Natalio Vita 9:30-10:10 Sanofi, France natalio.vita@tls1.elfsanofi.fr Neurotensin receptor pharmacology. -M BREAK 10:10-10:30 Damien O'Connell 10:30-11:10 Cork University, Ireland damian@ucc.ie Dopaminergic receptor activity in human proximal tubular cells as assessed by microphysiometry. -M Will Lachnit 11:10-11:50 Roche Biosciences, CA, USA wilheim.lachnit@roche.com =46LIPR: Quantitative pharmacological characterization of heterologously-expressed G-protein coupled receptors. - F LUNCH 11:50-1:00 Opening Remarks 1:00-1:10 Chairperson, Dr.Martyn Coldwell Smith-Kline Beecham, UK Signal Transduction John Raymond 1:10-1:50 Med. Univ. South Carolina, USA raymondj@musc.edu Use of the Cytosensor Microphysiometer System to map G-protein signalling pathways. Norbert Fraeyman 1:50-2:30 Heymans Institute, Belgium norbert.fraeyman@rug.ac.be Involvement of protein kinase C in the signal transduction related to M3-cholinergic receptor stimulation. Josh Rabinowitz 2:30-3:10 Stanford University, CA, USA rabino@leland.stanford.edu The Redox Microphysiometer: Linking Signal Transduction to Cellular Redox Metabolism - M BREAK 3:10-3:30 Novel Target Identification Jan Seelig 3:30-4:10 Biozentrum, Switzerland seelig1@ubaclu.unibas.ch New Drugs for the Na+/H+ Exchanger. Inhibition constants and detection of side effects.-M Mark Parmentier 4:10-4:50 Libre University, Belgium mparment@ulb.ac.be The identification and characterization of the chemokine receptor, CCR5.M Dr. Yasufuni Murakami 4:50-5:30 RIKEN Institute, JAPAN yasufumi@rtcmain.rtc.riken.go.jp Towards systemic analysis of gene functions of fully sequenced genome.M Tuesday Morning, April 29, 1997 Opening remarks 8:00-8:10 Chairperson, John Raymond, M.D. Medical University of South Carolina, USA Ion Channels Mark Wolfe 8:10-8:50 Pharmacia-Upjohn Co., MI, USA mark.l.wolfe@am.pnu.com Development of Cell Based High-volume Assays Using the FLIPR System. F David Rock 8:50-9:30 Parke-Davis Research, MI, USA rockd@aa.wl.com Monitoring voltage- and ligand-gated ion channels in cultured neurons using =46LIPR. - F Immunology Darren Smart 9:30-10:10 Parke-Davis, UK smartd@camb.wl.com IL-1 receptor characterization.-M BREAK 10:10-10:30 Dale Carey 10:30-11:10 CRC-CGF - WEHI, Australia carey@wehi.edu.au Analysis of Signalling Capacity of Truncation Mutants of the GM-CSF Receptor =DF-chain using the Cytosensor Microphysiometer - M Subhashani Arimilli 11:10-11:50 Anergen, Inc., CA, USA sarimilli@anergen.com Measurement of extracellular acdification rates in Transformed human T-cells to evaluate structure-function correlation of antigenic epitope by silicon microphysiometry. - M LUNCH 11:50-1:00 Opening Remarks 1:00-1:10 Chairperson, Dr. Simon Pitchford Applications Manager, Molecular Devices Corp. Tissues & Primary Cell Lines David Bristow 1:10-1:50 Manchester University, UK david.bristow@man.ac.uk Measurement of GABAA receptor function in cultured cerebellar granule cells using microphysiometry: a study of the mechanisms underlying benzodiazepine tolerance.-M Anthony Ford 1:50-2:30 Roche Biosciences, CA, USA anthony.ford@roche.com Pharmacological characterization of muscarinic cholinoceptors in rat submaxillary gland using microphysiometry.-M Richard Pittner 2:30-3:10 Amylin Pharmaceuticals, CA, USA rpittner@amylin.com Measurement of Acidification Rates and Hormone Secretion in Freshly Isolated Cells in Primary Culture.-M BREAK 3:10-3:30 Diagnostic Applications Bernard Fung 3:30-4:10 Jules Stein Eye Institute, UCLA, USA bkkfung@ucla.edu Real-time Monitoring of Reduced =DF-Adrenergic Responses in Fibroblasts from Patients with Pseudohypoparathyroidism.-M Julie Bennett-Desmelik 4:10-4:50 Emory University, GA, USA jbennett@gmm.gen.emory.edu Microphysiometric Analysis of Mitochondrial Function in Intact Lymphoblasts from Leber's Hereditary Optic Neuropathy.-M Wednesday Morning, April 30, 1997 9:00-12:30 Chairperson, Dr. Simon Pitchford Applications Manager, Molecular Devices Corp. Open discussion on any aspects of microphysiometry and fluorometric cellular screening. Topics for discussion to be submitted by audience. Poster sessions will run throughout the 3 days. We look forward to an exciting and stimulating conference in Paris, and hope you will have the opportunity to attend and participate. Jon Waterman-Smith European Biosensor Business Manager Molecular Devices Ltd Unit 6 Raleigh Court Rutherford Way Crawley W.Sussex RH10 2PD UK Tel: +44 1293 619579 =46ax: +44 1293 619586 email: jpwscyto@pavilion.co.uk From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 4 Apr 1997 16:42:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHBIBP95S0002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID qMTyAPuH2fiIqZYphKFmCA) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID qMTyAPuH2fiIqZYphKFmCA) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID qMTyAPuH2fiIqZYphKFmCA)-- From owner-7tms_r@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 4 Apr 1997 16:53:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHBIP5ZHLM002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID g/Er++Fcu9WelWx0mW+vIQ) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID g/Er++Fcu9WelWx0mW+vIQ) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA35PMBV40023JG@mc.duke.edu>; Thu, 03 Apr 1997 19:21:35 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03751; Thu, 03 Apr 1997 19:18:18 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28548; Thu, 03 Apr 1997 15:53:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28530; Thu, 03 Apr 1997 15:53:23 -0800 Date: Thu, 03 Apr 1997 17:40:41 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FF9.70C6@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID g/Er++Fcu9WelWx0mW+vIQ)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 18:23:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHD03PWGUI000046@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID 3M7P3GPyE9ar5NITRSbViw) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID 3M7P3GPyE9ar5NITRSbViw) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID 3M7P3GPyE9ar5NITRSbViw)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCR database update Date: 5 Apr 1997 03:01:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704051102.NAA17744@nu> NNTP-Posting-Host: net.bio.net Dear GPCR friends, Many of you have look already at the GPCRDB. Some of you are very regular users. This database can be found at: http://swift.embl-heidelberg.de/7tm/ Now that Florence Horn has joined us, we can really start working on the GPCRDB. Florence has several years of experience in database design, and it seems likely that her contribution will lead to a much better GPCRDB. Her first contribution is that the update frequency will now be once per two months, aiming at one update per day, a goal that we hope to reach within two years. On April 2-4 1997 the GPCRDB was updated. Much of this update was now done automatically, but as many of the programs used were used for the first time, some errors seem likely. Please report any errors that are not yet documented in the pages themselves. - We have added several hundred models. - We have added several thousands of ligand binding constants (a kind gift from Philip Seeman, thanks). - we have added models for the Class B receptors - We have added viseur based snake-like plots for all Class A receptors, and these plots are hyperlinked to the GRAP mutation database (thanks to Fabien Campagne and Oyvind Edvardson) - We have made the navigation through sequences and alignments more uniform. (We means Florence, her Email address is Horn@EMBL-Heidelberg.DE) Gert Vriend From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 18:13:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHCZR21G98000046@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID E/JnTY7b89Gac6NEJ7o12w) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID E/JnTY7b89Gac6NEJ7o12w) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID E/JnTY7b89Gac6NEJ7o12w)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 14:36:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHCS6OSGDM000043@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID ekUhwUGFUF/i2cY+IgUiew) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID ekUhwUGFUF/i2cY+IgUiew) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA3OM5BLS002YUV@mc.duke.edu>; Thu, 03 Apr 1997 19:36:46 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA04058; Thu, 03 Apr 1997 19:33:45 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27954; Thu, 03 Apr 1997 15:49:29 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27950; Thu, 03 Apr 1997 15:49:28 -0800 Date: Thu, 03 Apr 1997 17:39:43 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FBF.186F@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID ekUhwUGFUF/i2cY+IgUiew)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 14:18:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHCRJHEXVI000043@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID mMvMlfszWNTZnCmuXaKUCw) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID mMvMlfszWNTZnCmuXaKUCw) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA39ZWA00000LKM@mc.duke.edu>; Thu, 03 Apr 1997 19:25:00 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03837; Thu, 03 Apr 1997 19:21:42 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28525; Thu, 03 Apr 1997 15:53:21 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28520; Thu, 03 Apr 1997 15:53:19 -0800 Date: Thu, 03 Apr 1997 17:40:12 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FDC.2773@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID mMvMlfszWNTZnCmuXaKUCw)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 14:11:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHCRAE6U5O000043@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID UrtNhBlyhpBHwmD6h6CBEA) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID UrtNhBlyhpBHwmD6h6CBEA) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA35PMBV40023JG@mc.duke.edu>; Thu, 03 Apr 1997 19:21:35 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03751; Thu, 03 Apr 1997 19:18:18 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28548; Thu, 03 Apr 1997 15:53:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28530; Thu, 03 Apr 1997 15:53:23 -0800 Date: Thu, 03 Apr 1997 17:40:41 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FF9.70C6@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID UrtNhBlyhpBHwmD6h6CBEA)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 13:54:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHCQPTSZ6C000043@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID P2Q1b2YgLGLmXzvg2nV2IA) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID P2Q1b2YgLGLmXzvg2nV2IA) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID P2Q1b2YgLGLmXzvg2nV2IA)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 03:57:50 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHC5VISOVE002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID EJYBA4NDFKSb2X8nD7CRGQ) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID EJYBA4NDFKSb2X8nD7CRGQ) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA3OM5BLS002YUV@mc.duke.edu>; Thu, 03 Apr 1997 19:36:46 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA04058; Thu, 03 Apr 1997 19:33:45 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27954; Thu, 03 Apr 1997 15:49:29 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27950; Thu, 03 Apr 1997 15:49:28 -0800 Date: Thu, 03 Apr 1997 17:39:43 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FBF.186F@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID EJYBA4NDFKSb2X8nD7CRGQ)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 03:41:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHC5ATI066002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID Nbdcj61/UBkkVxO2LF7C1A) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID Nbdcj61/UBkkVxO2LF7C1A) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA39ZWA00000LKM@mc.duke.edu>; Thu, 03 Apr 1997 19:25:00 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03837; Thu, 03 Apr 1997 19:21:42 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28525; Thu, 03 Apr 1997 15:53:21 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28520; Thu, 03 Apr 1997 15:53:19 -0800 Date: Thu, 03 Apr 1997 17:40:12 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FDC.2773@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID Nbdcj61/UBkkVxO2LF7C1A)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 03:34:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHC523JLLO002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID lyPhhWarC5hoU/VciapGfQ) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID lyPhhWarC5hoU/VciapGfQ) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA35PMBV40023JG@mc.duke.edu>; Thu, 03 Apr 1997 19:21:35 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03751; Thu, 03 Apr 1997 19:18:18 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28548; Thu, 03 Apr 1997 15:53:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28530; Thu, 03 Apr 1997 15:53:23 -0800 Date: Thu, 03 Apr 1997 17:40:41 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FF9.70C6@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID lyPhhWarC5hoU/VciapGfQ)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 03:19:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHC4KIBHV8002OY3@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID trEMrmO5jfvrEBlm1ML2+g) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID trEMrmO5jfvrEBlm1ML2+g) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID trEMrmO5jfvrEBlm1ML2+g)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 18:37:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHD0KYGUBI000046@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID SzO0nY8sL/TmZuBxspWS/w) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID SzO0nY8sL/TmZuBxspWS/w) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA35PMBV40023JG@mc.duke.edu>; Thu, 03 Apr 1997 19:21:35 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03751; Thu, 03 Apr 1997 19:18:18 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28548; Thu, 03 Apr 1997 15:53:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28530; Thu, 03 Apr 1997 15:53:23 -0800 Date: Thu, 03 Apr 1997 17:40:41 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FF9.70C6@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID SzO0nY8sL/TmZuBxspWS/w)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 18:47:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHD0XC8CKG000046@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID VeowBp7xXIeKo0RK/Fz2Jg) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID VeowBp7xXIeKo0RK/Fz2Jg) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA39ZWA00000LKM@mc.duke.edu>; Thu, 03 Apr 1997 19:25:00 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03837; Thu, 03 Apr 1997 19:21:42 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28525; Thu, 03 Apr 1997 15:53:21 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28520; Thu, 03 Apr 1997 15:53:19 -0800 Date: Thu, 03 Apr 1997 17:40:12 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FDC.2773@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID VeowBp7xXIeKo0RK/Fz2Jg)-- From owner-7tms_r@net.bio.net Fri Apr 04 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 5 Apr 1997 19:03:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHD1HE7WY8000046@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID DADVjBRKjXa+wJPIaDSP+Q) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID DADVjBRKjXa+wJPIaDSP+Q) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA3OM5BLS002YUV@mc.duke.edu>; Thu, 03 Apr 1997 19:36:46 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA04058; Thu, 03 Apr 1997 19:33:45 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27954; Thu, 03 Apr 1997 15:49:29 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27950; Thu, 03 Apr 1997 15:49:28 -0800 Date: Thu, 03 Apr 1997 17:39:43 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FBF.186F@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID DADVjBRKjXa+wJPIaDSP+Q)-- From owner-7tms_r@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 8 Apr 1997 16:25:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHEZBSS1XM000042@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID pdsZ+gVB93PcZiBN+/2Z3Q) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID pdsZ+gVB93PcZiBN+/2Z3Q) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA39ZWA00000LKM@mc.duke.edu>; Thu, 03 Apr 1997 19:25:00 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03837; Thu, 03 Apr 1997 19:21:42 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28525; Thu, 03 Apr 1997 15:53:21 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28520; Thu, 03 Apr 1997 15:53:19 -0800 Date: Thu, 03 Apr 1997 17:40:12 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FDC.2773@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID pdsZ+gVB93PcZiBN+/2Z3Q)-- From owner-7tms_r@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!mayo.edu!Rao.Rammohan From: Rao.Rammohan@mayo.edu (R Rao) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Sincere apologies Date: 8 Apr 1997 16:06:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear 7tms_r netters/subscribers, With a right and sincere scientific earnest, I posted a mail to the above= =20 newsgroup regarding =D2Antagonist mediated internalization of a G protein= =20 coupled receptor=D3. Unfortunately the message has been repeating itself= =20 over and over, resulting in a flurry of the same message and cluttering=20 the e-mail of all subscribers.=20 I realize it is annoying, receiving similar messages over 20 times but=20 honestly, it is not a deliberate attempt in glorifying our publication=20 or our work.=20 I notified our institution=D5s Computing facility and after a thorough=20 check, I was informed that the problem is not from our side. I am told=20 that somewhere down the line the network went haywire resulting in these=20 erroneous repeat messages. I have informed repeatedly the 7tm newsgroup=D5s help desk and I hope they= =20 are rectifying the problem. Until then, kindly tolerate this barrage of=20 recurrent messages.=20 I also appeal to one and all to desist from sending me abusive letters.=20 It is unbecoming of Lecturers/Asst Professors/Professors, who write=20 disdainful letters that carry crude and raw language.=20 Finally I hope that this message does not travel the same way as my last on= e Rao. From owner-7tms_r@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 8 Apr 1997 15:08:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHEZ2B7EJ4000042@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID nO9jnDjzMcyKY/+YHc2E5Q) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID nO9jnDjzMcyKY/+YHc2E5Q) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA35PMBV40023JG@mc.duke.edu>; Thu, 03 Apr 1997 19:21:35 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03751; Thu, 03 Apr 1997 19:18:18 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28548; Thu, 03 Apr 1997 15:53:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA28530; Thu, 03 Apr 1997 15:53:23 -0800 Date: Thu, 03 Apr 1997 17:40:41 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FF9.70C6@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID nO9jnDjzMcyKY/+YHc2E5Q)-- From owner-7tms_r@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 8 Apr 1997 06:48:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHEZVGX0UM000042@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID 1fZrM1JK/L3ySjsQ3JgKpg) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID 1fZrM1JK/L3ySjsQ3JgKpg) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA3OM5BLS002YUV@mc.duke.edu>; Thu, 03 Apr 1997 19:36:46 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA04058; Thu, 03 Apr 1997 19:33:45 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27954; Thu, 03 Apr 1997 15:49:29 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27950; Thu, 03 Apr 1997 15:49:28 -0800 Date: Thu, 03 Apr 1997 17:39:43 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FBF.186F@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID 1fZrM1JK/L3ySjsQ3JgKpg)-- From owner-7tms_r@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!mayo.edu!Rao From: Rao@mayo.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 8 Apr 1997 06:01:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHEYODZMX4000042@mc.duke.edu> NNTP-Posting-Host: net.bio.net --Boundary (ID H70BLRDwMJPZKBMXAbqYCw) Content-type: TEXT/PLAIN We have recently reported internalization of CCK receptor by an antagonist. The paper entitled" Antagonist stimulated internalization of the G protein coupled CCK receptor appears as a Accelerated Communication in the March issue of Molecular Pharmacology Vol:51 Pg 357-362 --Boundary (ID H70BLRDwMJPZKBMXAbqYCw) Content-type: TEXT/PLAIN RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA2ZRM2GW002MXH@mc.duke.edu>; Thu, 03 Apr 1997 19:16:45 -0500 (EST) Received: from net.bio.net (net.bio.net [204.31.212.2]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id TAA03646; Thu, 03 Apr 1997 19:13:50 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27937; Thu, 03 Apr 1997 15:49:22 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id PAA27932; Thu, 03 Apr 1997 15:49:22 -0800 Date: Thu, 03 Apr 1997 17:39:18 -0600 From: Ram Subject: Re: non-internalizing receptors To: 7tms_r@net.bio.net To: roth@BIOCSERVER.BIOC.CWRU.EDU Message-id: <33443FA6.606@mayo.edu> MIME-version: 1.0 X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit NNTP-posting-host: millerl.mayo.edu --Boundary (ID H70BLRDwMJPZKBMXAbqYCw)-- From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: flaming Date: 9 Apr 1997 08:59:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704091600.SAA09556@nu> NNTP-Posting-Host: net.bio.net I suggest this is the last message send to the list about Rao's mail problem. He apologised for something that probably wasn't even his fault. That is enough. If not, mail to him, or to me PRIVATELY. Don't cluther this list. I spend a lot of time chasing people from outside the GPCR community who deposite garbage (this last set of four porno adds from last week we traced down to Finland, and the manager of the system where the dirt came from apologised and promised that repeats will not happen), and don't want to now also have to get our own people to look after. So DONT use 'reply' when you are angry, but send a directed Email. Gert Vriend From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!mindspring!cssun.mathcs.emory.edu!news.service.emory.edu!news From: Kelly Hartline Newsgroups: bionet.molbio.proteins.7tms_r Subject: Advice on class debate topics Date: Wed, 09 Apr 1997 10:35:09 -0400 Organization: Emory University School of Medicine Lines: 24 Message-ID: <334BA91D.7099@emory.edu> Reply-To: khartli@emory.edu NNTP-Posting-Host: dlk-lab1-4007.wmb.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; U) Hi! I'm a second year grad student in the neuroscience program at Emory University, and in my Cell Surface Receptors class, we have two topics to debate over the next two weeks. 1. The structure of bacteriorhodopsin is a good model for G protein-linked receptors. 2. Transgenic knockouts of receptors do not give more information than can be obtained with selective antagonists. Specifically, I've been assigned to the 'pro' side for the first topic, and the 'con' side for the second topic. I've begun scanning Medline for relevant editorials, reviews, etc., but I would appreciate any thoughts/opinions that any of you have to offer on the above subjects. I would also love to hear of any particular aspects of the two above issues that you feel would be interesting for me to examine/focus in on. Thanks in advance, Kelly Hartline (khartli@emory.edu) From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!MED.UNR.EDU!buxton From: buxton@MED.UNR.EDU ("Iain L. O. Buxton") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Sincere apologies Date: 9 Apr 1997 07:27:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net So Rao, thought you would like to know that in addition to this missive from you today, I got another, yet different appology copied to me along with another four of the original banal messages that you keep telling us you are not responsible for........have a nice day will you, after all, reading all this RAO RAMMOHAN mail is a great pleasure for us. PS: How's the weather in Rochester...? Iain L. O. Buxton Professor of Pharmacology/318 Associate Dean for Research University of Nevada School of Medicine Howard Medical Sciences Building Rm:216 Reno, NV 89557 Phone: (702)784-1566 FAX: (702)784-1620 buxton@med.unr.edu On 8 Apr 1997, R Rao wrote: > > Dear 7tms_r netters/subscribers, > > With a right and sincere scientific earnest, I posted a mail to the above= > =20 > newsgroup regarding =D2Antagonist mediated internalization of a G protein= > =20 > coupled receptor=D3. Unfortunately the message has been repeating itself= > =20 > over and over, resulting in a flurry of the same message and cluttering=20 > the e-mail of all subscribers.=20 > > I realize it is annoying, receiving similar messages over 20 times but=20 > honestly, it is not a deliberate attempt in glorifying our publication=20 > or our work.=20 > > I notified our institution=D5s Computing facility and after a thorough=20 > check, I was informed that the problem is not from our side. I am told=20 > that somewhere down the line the network went haywire resulting in these=20 > erroneous repeat messages. > > I have informed repeatedly the 7tm newsgroup=D5s help desk and I hope they= > =20 > are rectifying the problem. Until then, kindly tolerate this barrage of=20 > recurrent messages.=20 > > I also appeal to one and all to desist from sending me abusive letters.=20 > It is unbecoming of Lecturers/Asst Professors/Professors, who write=20 > disdainful letters that carry crude and raw language.=20 > > Finally I hope that this message does not travel the same way as my last on= > e > > Rao. > > From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!MED.UNR.EDU!buxton From: buxton@MED.UNR.EDU ("Iain L. O. Buxton") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Sincere apologies Date: 9 Apr 1997 07:22:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Hey Rao, if you cann't take the heat get out of the kitchen. You do bot get to harrass us with your uncontrollable e-mail, blame it on others and then cry when you're called on it. Get it! Iain L. O. Buxton Professor of Pharmacology/318 Associate Dean for Research University of Nevada School of Medicine Howard Medical Sciences Building Rm:216 Reno, NV 89557 Phone: (702)784-1566 FAX: (702)784-1620 buxton@med.unr.edu On 8 Apr 1997, R Rao wrote: > > Dear 7tms_r netters/subscribers, > > With a right and sincere scientific earnest, I posted a mail to the above= > =20 > newsgroup regarding =D2Antagonist mediated internalization of a G protein= > =20 > coupled receptor=D3. Unfortunately the message has been repeating itself= > =20 > over and over, resulting in a flurry of the same message and cluttering=20 > the e-mail of all subscribers.=20 > > I realize it is annoying, receiving similar messages over 20 times but=20 > honestly, it is not a deliberate attempt in glorifying our publication=20 > or our work.=20 > > I notified our institution=D5s Computing facility and after a thorough=20 > check, I was informed that the problem is not from our side. I am told=20 > that somewhere down the line the network went haywire resulting in these=20 > erroneous repeat messages. > > I have informed repeatedly the 7tm newsgroup=D5s help desk and I hope they= > =20 > are rectifying the problem. Until then, kindly tolerate this barrage of=20 > recurrent messages.=20 > > I also appeal to one and all to desist from sending me abusive letters.=20 > It is unbecoming of Lecturers/Asst Professors/Professors, who write=20 > disdainful letters that carry crude and raw language.=20 > > Finally I hope that this message does not travel the same way as my last on= > e > > Rao. > > From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms_r: HIV and opiates, summary Date: 9 Apr 1997 01:41:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 115 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, One week back, I posted a question about possible interaction of opiates with chemokine receptor proteins and possible mechanisms of activation of HIV infection by morphine and other narcotics. Here it is the answers. It seems that nobody measured direct binding of opiates to chemokine receptors and nobody knows if such kind of interaction may or may not take place... One of the possible mechanisms could be the influence of opiates on cellular immune responses through opioid receptors and cellular signaling systems... Many thanks for everyone who answered. Gennady 1 ---------------------------------------------------------------------- Dear Dr. Poda: I am intrigued with the question you pose. Faced with a not so disimilar issue with respect to muscarinic systems and NO synthase inhibitors, we show that a not-so-high-affinity interaction can be an issue (Buxton, I.L.O., Cheek, D.J., Eckman, D.M., Westfall, D.P. Sanders, K.M. and Keef, K.D. (1993) NG-nitro-L-arginine methylester (L-NAME) and other alkyl esters of arginine are muscarinic receptor antagonists. Circ. Res. 72: 387-395.) We could look at this with you if you are interested? Iain L. O. Buxton Professor of Pharmacology/318 Associate Dean for Research University of Nevada School of Medicine Howard Medical Sciences Building Rm:216 Reno, NV 89557 Phone: (702)784-1566 FAX: (702)784-1620 buxton@med.unr.edu 2 ----------------------------------------------------------------------- One thought I have is effects down-stream of the opiate receptor, and their influence on cellular immune responses. I wouldn't bet the house on interactions with CC receptor proteins. An interesting paper to look at follows, if for any reason because it can serve as an extensively referenced entry into the literature of how GPCR's linked to adenylyl cyclase can influence lymphocyte function. Condie, R., A. Herring, W. S. Koh, M. Lee, and N. E. Kaminski. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin-2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271(22):13175-13183 (1996). -- T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology http://www.emory.edu/PHARMACOLOGY/MURPHY/ 1510 Clifton Road Atlanta, GA 30322 ---------------------------------------------------------------------------- The question is here: From poda@violet.incm.u-nancy.frWed Apr 9 10:38:20 1997 Date: 2 Apr 1997 02:00:38 -0800 From: Gennady PODA To: 7tms_r@net.bio.net Subject: 7tms_r: HIV and opiates Dear Netters, It is known that morphine and other narcotics alter the function of human T lymphocytes and monocytes and may lead to activation of HIV infection (ex., Squinto SP, et al. AIDS Res Hum Retroviruses, 6(10):1163-1168, 1990). Moreover, potentiation of HIV growth by morphine was antagonized by naloxone and beta-funaltrexamine (opioid antagonists) (Peterson PK, et al. AIDS 4(9):869-873, 1990). This means that opiate systems involved somehow in HIV pathogenesis. Did anyone investigate possible mechanisms? One of the possibilities can be direct binding of opiates to chemokine receptors (found to be cofactors for HIV and host cell membrane fusion). So the question, has anyone seen (or measured) binding constants of opiate ligands (antagonists or agonists) to the chemokine receptors such as CXCR-4 and CCR-5, etc.? This binding may be rather non specific but even so... Any hints or references would be greatly appreciated. I will summarize the answers. Best wishes and many thanks for your participation, Gennady ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!biochem.umass.edu!bscott From: bscott@biochem.umass.edu (Brian Scott) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Free molecular visualization workshops for profs Date: 9 Apr 1997 17:49:08 -0700 Organization: University of Massachusetts Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <334C38C5.37FA@biochem.umass.edu> Reply-To: bscott@biochem.umass.edu NNTP-Posting-Host: net.bio.net Free Workshops on Molecular Visualization for Undergraduate Bioscience Teaching Amherst MA -- Summer, 1997-8 Understanding the three-dimensional structures of proteins, DNA, RNA, and their interactions is difficult from flat pictures, yet grasping structure is important to understanding function. Free software is now available which displays attention-grabbing 3D animated images of biological molecules in depth-cued spacefilling color. RasMol (http://www.umass.edu/microbio/rasmol), which works on Windows or Macs, encourages self-directed exploration. Chime (http://www.umass.edu/microbio/chime) allows annotated preset views of molecules to be delivered as web tutorials. Free, hands-on workshops will be held this summer at the University of Massachusetts in Amherst to prepare college faculty with no prior experience to use molecular visualization in their classes. Participants will travel to the Amherst campus on three separate days (two this summer, one the following summer), and are responsible for their own travel expenses (and therefore are expected primarily from the Northeastern USA). Overnight accomodations will be provided free to those needing them (based on distance traveled). Participants will be expected to incorporate molecular visualization into their teaching, and to mentor two faculty colleagues at their home institution. The workshops will be led by Eric Martz, a Professor in the Department of Microbiology. Martz has innovated molecular structure tutorials which are in use throughout the USA and in dozens of other countries. The web site he created (see above) was visited by 40,000 people in 1996. 1997 dates are June 17 and 30 (workshop A), or June 19 and July 2 (workshop B). Workshops A and B also meet for one day in late June 1998. To obtain more detailed information and a REGISTRATION FORM, visit http://www.umass.edu/microbio/rasmol/workshop.htm or FAX a request to 413-545-1578, or email a request to emartz@microbio.umass.edu. Supported by the National Science Foundation (Undergraduate Faculty Enhancement, Division of Undergraduate Education) and the University of Massachusetts. /* - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor (Immunology), Dept Microbiology, Univ Massachusetts, Amherst MA US 01003-5720 413-545-2325 FAX:545-1578. RasMol Home Page, http://www.umass.edu/microbio/rasmol Other web projects listed at http://www.umass.edu/microbio/rasmol/em-web.htm - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/ From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!news.apfel.de!fu-berlin.de!wuff.mayn.de!wuff.franken.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Advice on class debate topics Date: Wed, 9 Apr 1997 21:20:22 +0200 Organization: University of Wuerzburg, Germany Lines: 67 Message-ID: References: <334BA91D.7099@emory.edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Kelly Hartline (khartli@emory.edu) wrote: > Hi! > > I'm a second year grad student in the neuroscience program at Emory > University, and in my Cell Surface Receptors class, we have two topics > to debate over the next two weeks. > > 1. The structure of bacteriorhodopsin is a good model for G > protein-linked receptors. Since you have been assigned the "pro" side for this topic, you should check out articles by Harel Weinstein. I have a few references for you (there are more, of course): @article{pardo:92, author = {Leonardo Pardo and Juan Antonio Ballesteros and Roman Osman and Harel Weinstein}, title = {On the use of the transmembrane domain of bacteriorhodopsin as a template for modeling the three-dimensional structure of guanine nucleotide-binding regulatory protein-coupled receptors.}, journal = PNAS, volume = 89, pages = {4009--4012}, year = 1992 } @article{dzhang:94, author = {Daqun Zhang and Harel Weinstein}, title = {Polarity conserved positions in transmembrane domains of {G}-protein coupled receptors and bacteriorhodopsin.}, journal = FEBS, volume = 337, pages = {207--212}, year = 1994 } Unfortunately it seems that the projection structure of frog rhodopsin is dissimilar to bacteriorhodopsin. At least that is what Gebhardt Schertler (who did all these rhodopsin structures) claims. This has been debated in @article{hoflack:94, author = {Jan Hoflack and Suzanne Trumpp-Kallmeyer and Marcel Hibert}, title = {Re-evaluation of bacteriorhodopsin as a model for {G} protein-coupled receptors.}, journal = TIPS, volume = 15, pages = {7--9}, year = 1994 } > 2. Transgenic knockouts of receptors do not give more information than > can be obtained with selective antagonists. The obvious problem is that you have to have selective antagonists, which you often don't have. Hope that helps a little, --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Tue Apr 08 23:00:00 1997 Path: biosci!EMORY.EDU!" smitc04" From: " smitc04"@EMORY.EDU (stephanie mitchell) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Please Help Me Date: 9 Apr 1997 12:50:45 -0700 Organization: Emory Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <334BF383.786E@emory.edu> Reply-To: smitc04@emory.edu NNTP-Posting-Host: net.bio.net Hello, I am a first year graduate student in the pharmacology program at Emory University and I have to debate Kelly on the same topics next week. Topics: 1. The structure of bacteriorhodopsin is not a good model for G- protein linked receptors. 2. Transgenic knockouts of receptors do give more information than can be obtained with selective antagonists. If you agree with the above topics and can send me any information to support them I would appreciate it. I have started a net search and have found very little information to support topic #1. Thanks in advance, Stephanie Mitchell From owner-7tms_r@net.bio.net Wed Apr 09 23:00:00 1997 Path: biosci!agate!howland.erols.net!europa.clark.net!newsfeed.nacamar.de!wuff.mayn.de!wuff.franken.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Advice on class debate topics Date: Thu, 10 Apr 1997 09:56:26 +0200 Organization: University of Wuerzburg, Germany Lines: 21 Message-ID: References: <334BA91D.7099@emory.edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Dan Strahs has alerted me that the second Weinstein paper I quoted, > @article{dzhang:94, > author = {Daqun Zhang and Harel Weinstein}, > title = {Polarity conserved positions in transmembrane domains > of {G}-protein coupled receptors and bacteriorhodopsin.}, > journal = FEBS, > volume = 337, > pages = {207--212}, > year = 1994 > } actually argues against a similarity between bacteriorhodopsin and the GPCRs. I apologize. (Never quote a paper from memory...) --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Apr 10 23:00:00 1997 Path: biosci!INKA.MSSM.EDU!hweinstein From: hweinstein@INKA.MSSM.EDU (Dr. Harel Weinstein) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 11 Apr 1997 21:45:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 64 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues, There seems to have been some confusion about the treatment given in our publications to the use of Bacteriorhodopsin as a template for GPCRs. I would like to offer a clarification of our current understanding, as it continue to be consistent with all the previous considerations. The two articles mentioned in recent correspondence here, i.e., Pardo et al, PNAS 1992, and Zhang & Weinsten, FEBS Letters, 1994 provide different types of data to support the same clear, and by now unassailable assertion that bacteriorhodopsin IS NOT a suitable structural model for the assembly of the 7 TMH bundle of GPCRs. The main, and conceptually quite straightforward reason is that the important differences in the amino acid sequences of BR and the GPCRs preclude a similarity in the bundling of the seven transmembrane helices in a pattern that could be similar in the two DIFFERENT families of membrane proteins (not that low homology per se is not a definitive criterion for lack of structural similarity, as many examples now exist of such cases, but if clearly defined differences can be shown to be responsible for divergent properties, then the lack of sequence homology can be expected safely to be accompanied by lack of structural homology). The main elements of the dissimilar sequence that are responsible for the difference in bundling center on the distribution of Proline residues among the TMHs in the two families, and the effect of the significant difference in the positions of the Pro even in the helices in which they appear in both Families. These issues are discussed in depth, with explicit and quantitative detail in the comprehensive review chapter we published (Ballesteros & Weinstein, Methods in Neurosciences Vol 25, (Ed. S.C. Sealfon) pp. 366-428, 1995.). The capital importance of the Pro for the structure of the TMHs, and their ability to interact in the bundle has been demonstrated experimentally in relation to the rhodopsin-based models, in a recent publication (Fu et al, Biochemistry, 35:11278-11285, 1996). The effect of the Pro-kink (or the more complex distortion produced by an Asn-Pro or Asp/Pro sequence) on a TMH, which is clearly demonstrated by the experimental results, also clarifies the often encountered confusion produced by the use of ideal alpha-helices in models of GPCR TMH bundles (e.g., the erroneous "inability" of such inadequate ideal models to account for a number of experimentally observed helix-helix interactions, such as the TMH2-TMH7 interaction described in Zhou et al, Mol. Pharmacol. 45:165-170,1994 and Sealfon et al, J. Biol. Chem. 270:16683-16688, 1995). The inappropriate use of ideal alpha-helices in Pro-containing helices of models of the TMH bundle, regardless of the manner in which the individual helices and their orientations (i.e., lipid face vs. interior face) were determined, seems to have lead to strange and misleading conclusions. Given the conceptual inadequacy of such simplistic helix constructs, and the clear experimental evidence against them, it seems to us in this Lab, that their continued use is unadvisable. I hope this is useful and that it may generate some good discussion. Best regards to all, HW ---------------------------------------------------------------------------- ---------------------------------- Dr. Harel Weinstein Professor and Chairman Department of Physiology and Biophysics Box 1218 Mount Sinai School of Medicine New York, NY 10029-6574 (212) 241-7018 Fax 212 860 3369 WW Web Page http://www.mssm.edu/physbio/local/primfac/weinstein.html From owner-7tms_r@net.bio.net Fri Apr 11 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Apr 1997 02:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704120900.CAA09644@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. 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Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! 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If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Fri Apr 11 23:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: proline kinks Date: 12 Apr 1997 07:14:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704121415.OAA13811@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net Harel Weinstein recently stated that: "The capital importance of the Pro for the structure of the TMHs, and their ability to interact in the bundle has been demonstrated experimentally in relation to the rhodopsin-based models, in a recent publication (Fu et al, Biochemistry, 35:11278-11285, 1996)." >From the outset, I'd like to say that I do agree with the general conclusions of the Fu et al article and that we have data (submitted) that agrees with the general orientation of residues in Helix VII and that this new insight certainly clarifies some important experimental findings which were confusing. Thus said, it should be emphasized that the Fu article, although elegant in design and interpretation, really gives no direct evidence for the model proposed. The study is essentially a cys-substitution accessibility study in which hydrophilic cysteine reagents are used to inhibit ligand binding. This study proposes, based on unexpected findings, that there is a pronounced 'kink' introduced by a Pro in Helix VII. A rather trivial interpretation for the results is that cys-substitution non-specifically alters helical packing and/or accessibility. Without detailed structural studies of the type which are currently unavailable it will be difficult to rule out many of these types of artifacts which can confound the interpretation of the data. What is important about the Fu et al study is that it gives a plausible model to account for some unexpected findings. I think its a bit premature, however, to argue that these results *directly* support the notion that Pro kinks actually occur in 7-TM receptors. What I believe all of this reinforces, though, is the necessity for structural studies of 7-TM receptors. Bryan Roth ============================================================= Bryan L.Roth MD, PhD Associate Professor Departments of Psychiatry, Biochemistry and Neurosciences Room W438 Case Western Reserve University Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 216-368-4544 (Fax) 216-368-2730 (Office) roth@biocserver.cwru.edu web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html From owner-7tms_r@net.bio.net Mon Apr 14 23:00:00 1997 Path: biosci!SHOWME.MISSOURI.EDU!c680296 From: c680296@SHOWME.MISSOURI.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 15 Apr 1997 09:28:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net SUBSCRIBE From owner-7tms_r@net.bio.net Mon Apr 14 23:00:00 1997 Path: biosci!SHOWME.MISSOURI.EDU!c680296 From: c680296@SHOWME.MISSOURI.EDU ("David M. Bourdon") Newsgroups: bionet.molbio.proteins.7tms_r Subject: serotonin receptor newsgroup? Date: 15 Apr 1997 20:02:45 -0700 Organization: Dept. of Pharmacology - MU School of Medicine Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <33545EC6.257A@showme.missouri.edu> NNTP-Posting-Host: net.bio.net Does anyone know of a serotonin receptor newsgroup on the internet? I'm studying 5-HT GPCR subtypes in peripheral tissues of the rat. -- DAVID M. BOURDON -- Contact Information Graduate Research Assistant Department of Pharmacology, M517B Med.Sci.Bldg. MU School of Medicine, Columbia, MO 65212 USA phone: 573-882-1554 fax: 573-884-4558 home page: http://www.missouri.edu/~c680296/index.html From owner-7tms_r@net.bio.net Tue Apr 15 23:00:00 1997 Path: biosci!news.ohsu.edu!NewsWatcher!user From: forte@ohsu.edu (Michael Forte) Newsgroups: bionet.molbio.proteins.7tms_r Subject: CHO Cells Date: 16 Apr 1997 21:43:33 GMT Organization: Vollum Institute Lines: 7 Message-ID: NNTP-Posting-Host: 137.53.98.58 Hi, I know this issue has been raised in this group before, but of course I didn't care then. Can anyone tell me or point me to a reference concerning the spectrum of Galpha proteins expressed in CHO cells. Thanks. Mike Forte From owner-7tms_r@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!newsfeed.nacamar.de!fu-berlin.de!news-ber1.dfn.de!news-ham1.dfn.de!news-han1.dfn.de!news-koe1.dfn.de!news.ruhr-uni-bochum.de!news.rhrz.uni-bonn.de!usenet From: R.B.Jansen@Uni-Bonn.de (Raymond "ElRay" B. Jansen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: preotein sequencing..............urgent! Date: Thu, 17 Apr 1997 11:16:09 GMT Organization: Universitaet Bonn, Botanisches Institut Lines: 17 Message-ID: <33560666.11731026@news.rhrz.uni-bonn.de> NNTP-Posting-Host: rhrz-ts1-p12.rhrz.uni-bonn.de X-Newsreader: Forte Free Agent 1.1/32.230 Dear colleagues, I am desperately looking for a quick and reasonable opportunity to sequence my purified proteins. These proteins are bound to a PVDF-membrane, so, that they can cut off easily for sequencing. What I want to know exactly is, who (maybe a company) is able to do the job for me and what will be the price for this. If there is someone out and know the answers please contact me by e-mail.My address is: unb13c@ibm.rhrz.uni-bonn.de. I appreciate every help. Thanks in advance, Mechthild From owner-7tms_r@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!uunet!in3.uu.net!192.108.254.3!news.teleport.com!not-for-mail From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r Subject: how to subscribe/unsubscribe Date: Thu, 17 Apr 1997 15:21:48 GMT Organization: Oregon Health Sciences University Lines: 163 Message-ID: <33563f63.257251928@news.teleport.com> NNTP-Posting-Host: ip-pdx10-12.teleport.com X-Newsreader: Forte Free Agent 1.1/32.230 I have seen a number of futile subscribe/unsubscribe messages in this newsgroup recently, so perhaps it is time to re-post the instructions. The following is a message posted by Rick Neubig a year ago. _______________________________________________ You need to unsubscribe yourself. Here's some info about the 7tms_r newsgroup and about newsgroups in general. ------------------------------------------------------------------ The short answer re subscribing and unsubscribing in North America is to send a message with one line to biosci-server@net.bio.net To unsubscribe it should come from your old address and say: unsubscribe 7tms_r To subscribe it should come from your new address and say: subscribe 7tms_r More detailed information follows re BIOSCI newsgroup subscriptions. Cheers, Rick _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 E-mail Subscription Requests and other Information -------------------------------------------------- For users in the Americas and Pacific Rim countries, e-mail subscription and cancellation requests are handled automatically by an e-mail server, although personal assistance is also available via the biosci-help@net.bio.net address. Once your e-mail address is added to a subscription list, mail will be sent to your address automatically each time someone posts a message. This will continue until you remove your address from the subscription list. You should first send the lists command to the address biosci-server@net.bio.net to obtain a listing of all current BIOSCI mailing lists. 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Your e-mail address is obtained AUTOMATICALLY from the header of your mail message and should NOT be included on the subscribe/unsubscribe command line unless you need to sign up at a different address than the one you are currently using. Such requests must be handled manually by us and create extra work. (Also you would be surprised at how many people list their e-mail addresses incorrectly, so please let our server obtain this information automatically from your mail header.) Multiple commands may be placed on separate lines in the same mail message, i.e., you can include all of your subscribe and/or unsubscribe requests in one mail message as long as each is on a separate line. If you put multiple commands in your mail message, please put an end command as the last in your list of commands. This helps avoid sending to the server any signatures that might be automatically included in your mail messages. Here is a sample subscription message. 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E-mail Address Changes ---------------------- If you have subscribed to a newsgroup and are now leaving an institution or changing your e-mail address, it is IMPERATIVE that you cancel your subscription! Non-existent addresses or overflowing mailboxes cause computer mail programs to send back "daemon" messages which might bother everybody on the newsgroup. We will immediately remove any address causing such a problem, but would prefer it if you would notify us in advance as a courtesy to the rest of the user community. Interruption of E-mail Service ------------------------------ It is our policy to remove any address from our mailing lists which becomes inaccessible and causes mail to bounce back to the sender. This might happen to you if your local computer or network fails for a significant period of time. If you notice that you are no longer receiving BIOSCI postings, it may be because your address was removed for the above reason. It will be necessary for you to contact biosci-help@net.bio.net and resubscribe. Please see the BIOSCI FAQ, mentioned at the beginning of this document, for more details on how BIOSCI handles addresses which reject mail. ==================== end of BIOSCI info ==================== From owner-7tms_r@net.bio.net Thu Apr 17 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!sprint!howland.erols.net!newsxfer.itd.umich.edu!news.mtu.edu!msunews!netnews.upenn.edu!brass6.med.upenn.edu!user From: obrien@pharm.med.upenn.edu (:-Peter) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: CHO Cells Date: 18 Apr 1997 14:59:03 GMT Organization: U. of Pennsylvania Lines: 26 Message-ID: References: NNTP-Posting-Host: brass6.med.upenn.edu In article , forte@ohsu.edu (Michael Forte) wrote: @ Hi, @ @ I know this issue has been raised in this group before, but of course I @ didn't care then. Can anyone tell me or point me to a reference @ concerning the spectrum of Galpha proteins expressed in CHO cells. Thanks. @ @ Mike Forte Mike, A useful tool for looking up old usenet posts is called Dejanews. Point your web browser to http://www.dejanews.com/ One can search for previously posted articles using keywords, group names etc. It cuts down on the reiteration of topics that have been covered in the past, thus saving a bit of that mythical, precious "bandwidth." :-Peter From owner-7tms_r@net.bio.net Fri Apr 18 23:00:00 1997 Path: biosci!EMORY.EDU!hawad From: hawad@EMORY.EDU (Hazar Awad) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Transgenic KO's vs. selective antagonists. Date: 19 Apr 1997 08:34:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi. I am a first year graduate student in the pharmacology program at Emory University. I have been assigned the following debate topic for my Cell Surface Receptors class: -Transgenic knockouts of receptors do not give more information than can be obtained with selective antagonists. Any thoughts on this topic would be greatly appreciated. Hazar Awad. hawad@emory.edu From owner-7tms_r@net.bio.net Sat Apr 19 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-xfer.netaxs.com!cronkite.ocis.temple.edu!astro.temple.edu!not-for-mail From: driska@astro.temple.edu (Stephen P. Driska PhD) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: non-internalizing receptors Date: 17 Apr 1997 19:48:54 GMT Organization: Temple University, Academic Computer Services Lines: 32 Distribution: world Message-ID: <5j5ur6$j7f@cronkite.ocis.temple.edu> References: <01IHEZBSS1XM000042@mc.duke.edu> NNTP-Posting-Host: astro.ocis.temple.edu NNTP-Posting-User: 536870664 X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Rao@mayo.edu wrote: 16 times! that .... : We have recently reported internalization of CCK receptor by an : antagonist. The paper entitled" Antagonist stimulated internalization : of the G protein coupled CCK receptor appears as a Accelerated : Communication in the March issue of Molecular Pharmacology : Vol:51 Pg 357-362 : What causes these repeat messages? Is it someone posting from a newsreader like tin, not seeing the posting immediately, and repeatedly trying to post again? Some advice if this is the problem: If you're using tin to read the news, you may need to leave the newsgroup and then re-enter it before your post will be shown.... Just an idea...... Steve -- Steve Driska, Physiology Department, Temple University Medical School Philadelphia, PA 19140 USA (215) 707-3283 driska@astro.ocis.temple.edu From owner-7tms_r@net.bio.net Sat Apr 19 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Alessandro Giolitti Newsgroups: bionet.molbio.proteins.7tms_r Subject: Paper on Bradykinin receptor purification Date: 21 Apr 1997 06:39:19 +0100 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5jeui8$q2l@mserv1.dl.ac.uk> X-Sender: lns@mail.dada.it Original-To: 7tms_r@dl.ac.uk Dear netters, I have only recently found the paper by M. Yaqoo and C.R.Snell "Purification and Characterization of B2 Bradykinin Receptor from Rat Uterus", on J. Neurochem. 1994, 62(1), 17-26. I was wondering what is the opinion other people has about this work. Since it seems that the solubilized receptor mantains most of the binding ability for bradykinin and antagonists, and a high specificity, it looks like it is also mantaining its tertiary structure. What do the 7tm community thinks about? Thanks, Alessandro Giolitti ____________________________________________________________ Dr. Alessandro Giolitti Drug Design Dept. Ph. : +39-55-5680240 Menarini Ricerche S.p.A. FAX : +39-55-5680419 Via Sette Santi, 3 e-mail: lns@dada.it 50131 Firenze Italy ____________________________________________________________ From owner-7tms_r@net.bio.net Tue Apr 22 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!newsxfer3.itd.umich.edu!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!psgrain!news.rain.net!atheria.europa.com!isdn4.europa.com!user From: paulw@nwneuro.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: Combinatorial library determination Date: 23 Apr 97 20:00:13 GMT Organization: Europa Communications Inc. [Portland, OR] Lines: 3 Message-ID: NNTP-Posting-Host: atheria.europa.com Does anyone have a source of information to determine which types of combinatorial libraries are more likely to interact with g-protein linked receptors? From owner-7tms_r@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!pr.cyanamid.com!ramaswam From: ramaswam@pr.cyanamid.com (Ramaswamy Nilakantam) Newsgroups: bionet.molbio.proteins.7tms_r Subject: combinatorial library Date: 24 Apr 1997 08:56:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704241555.AA149707308@primail.pr.cyanamid.com> NNTP-Posting-Host: net.bio.net paulw wrote: Does anyone have a source of information to determine which types of combinatorial libraries are more likely to interact with g-protein linked receptors? You might try a small company called Pharmacopeia, in Princeton, NJ. They are in the business of designing combinatorial libraries. You might be able to get the info' you are looking for from someone there without actually buying anything. Worth a try anyway. Ramaswamy Nilakantan Wyeth-Ayerst Research Pearl River, NY From owner-7tms_r@net.bio.net Sun Apr 27 23:00:00 1997 Path: biosci!FARMR1.MED.UTH.TMC.EDU!aschonb From: aschonb@FARMR1.MED.UTH.TMC.EDU ("A. Schonbrunn") Newsgroups: bionet.molbio.proteins.7tms_r Subject: endogenous receptors Date: 28 Apr 1997 15:32:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I periodically continue to receive requests for the list of receptors expressed in cells commonly used in transfection studies and have now posted my most recent compillation at: http://www.biomedcomp.com/GPCR.html I will continue to update this list, so please send additions & corrections directly to me and I will make the necessary modifications. Thanks to everyone who provided input - your name should be listed on the web page: again let me know if I've inadvertantly omitted someone. as ************************************************************************** Agnes Schonbrunn, Ph.D. e-mail: aschonb@farmr1.med.uth.tmc.edu Professor FAX: 713-500-7456 Dept of Integrative Biology, Pharmacology & Physiology Univ. of Texas Medical School P.O.Box 20708, Houston,TX 77225 From owner-7tms_r@net.bio.net Mon Apr 28 23:00:00 1997 Path: biosci!niob.knaw.nl!domain From: domain@niob.knaw.nl Newsgroups: bionet.molbio.proteins.7tms_r Subject: changed domain Date: 29 Apr 1997 00:22:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704290722.AAA10839@net.bio.net> NNTP-Posting-Host: net.bio.net This is an automatic generated message ! Our email and WWW address has changed from : xxxxx@hubrecht.nl and www.hubrecht.nl to xxxxx@niob.knaw.nl and www.niob.knaw.nl e.g. jane@hubrecht.nl is now jane@niob.knaw.nl don't use hubrecht.nl or hl99.hubrecht.nl anymore -------------------------------------------------------------------------------- NIOB uppsalalaan 8, 3584CT Utrecht The Netherlands, ( Hubrecht laboratory ) EMAIL postmaster@niob.knaw.nl -------------------------------------------------------------------------------- From owner-7tms_r@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!NEWSSUN.MED.MIAMI.EDU!idickers From: idickers@NEWSSUN.MED.MIAMI.EDU (Ian Dickerson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 30 Apr 1997 13:09:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net How do I subscribe? Thanks, ian Dickerson From owner-7tms_r@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Position available Date: 1 May 1997 06:26:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705011327.NAA09462@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net A postdoctoral fellow position will be available in September in the Department of Biochemistry at Case Western Reserve University Medical School to study structure-activity relationships with 5-HT2-family receptors, Gq-like proteins and RGS proteins. Experience with protein purification is essential and expertise with baculovirus expression systems is desirable. The position is for two years with a posibility of a one- or two-year extension. Salary is negotiable and dependent upon qualifications. For further information contact Dr. Bryan Roth at the address below. More information may be obtained at the Web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html ============================================================= Bryan L.Roth MD, PhD Associate Professor Departments of Psychiatry, Biochemistry and Neurosciences Room W438 Case Western Reserve University Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 216-368-4544 (Fax) 216-368-2730 (Office) roth@biocserver.cwru.edu web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html From owner-7tms_r@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!UCHICAGO.EDU!g-bell From: g-bell@UCHICAGO.EDU (Graeme Bell) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 1 May 1997 06:42:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!WPMAIL.PAISLEY.AC.UK!samb-bs0 From: samb-bs0@WPMAIL.PAISLEY.AC.UK (Balwinder Sambi) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCRs Date: 1 May 1997 02:27:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know what endogenous