From owner-7tms_r@net.bio.net Wed Dec 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: newsmgr@merrimack.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: US-NJ-JOB OPPORTUNITY WITH G PROTEIN COUPLED RECEPTORS Date: 4 Dec 1997 09:24:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <666ovh$n23@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chasm Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!206.214.99.1!ix.netcom.com!news Newsgroups: bionet.molbio.proteins.7tms_r Subject: US-NJ-JOB OPPORTUNITY WITH G PROTEIN COUPLED RECEPTORS Message-ID: <01bcff51$333cfcc0$LocalHost@scott-s-armada> From: "Scott Shanes" Date: 2 Dec 1997 19:13:47 GMT Organization: Netcom Lines: 16 NNTP-Posting-Host: trn-nj4-18.ix.netcom.com X-NETCOM-Date: Tue Dec 02 1:13:47 PM CST 1997 X-Newsreader: Microsoft Internet News 4.70.1161 I am looking for a Scientist to work on G Protein Coupled Receptors . This person will be working on Radio Ligand Binding Assays and Second Messenger Assays. The selected candidates' job will be 50% basic science and 50% applied science drug discovery. This person will be a part of a larger group in drug discovery and will be supervising three people. This person should have excellent communication skills and computer skills (word processing, data analysis, excel, etc.). You should possess a PhD in Pharmacology, Biochemistry, Neuroscience or related field with 1 year postdoctoral experience. Our client is a leading pharmaceutical company with research facilities in Northern New Jersey and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax CV and cover letter to 609-584-9575 or E-Mail to sis@diedremoire.com From owner-7tms_r@net.bio.net Wed Dec 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: saudekv@synbio.tpgnet.net (Vladimir Saudek) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Protein Modeller Date: 4 Dec 1997 09:25:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <666p32$n7l@net.bio.net> NNTP-Posting-Host: net.bio.net Synthelabo Biomoleculaire has an immediate opening in the Group of Structural Biology for Protein Modeller The successful candidate will have PhD in structural biology, physical chemistry, theoretical chemistry or chemistry and several years of postdoctoral experience in a well-established protein modelling group. His/her skills will include Analysis of amino and nucleic acid sequences and sequence - function assignment Homology and threading modelling Molecular mechanics and dynamics Docking techniques Use of www modelling and sequence analysis resources Good knowledge of structural biology, familiarity with X-ray crystallography and NMR spectroscopy Ability to collaborate within a specialised protein structure team (X-ray crystallography, NMR and optical spectroscopy) as well as interact with scientists in a multidisciplinary institute (molecular biology, biochemistry, chemistry, genomics, bioinformatics) Excellent communication Integrate in an international team Synthelabo Biomoleculaire, a research institute located in Strasbourg and a subsidiary of the French pharmaceutical company Synthelabo, performs research towards the rapid generation of leads for new therapeutic targets emerging from the human genome. The Group of Structural Biology is equipped with 600, 500 and 360 MHz NMR spectrometers, X-ray diffractometer and a range of optical spectrometers (UV, IR, Raman, CD, fluorescence). The computing environment is based on Silicon Graphics servers and workstations. The group collaborates closely with molecular biology, genomics, bioinformatics, and medicinal and combinatorial chemistry. If you are interested, please send your CV and a letter of interest (preferably by email) to the address below. Vladimir Saudek Structural Biology Synthelabo Biomoleculaire 16, rue d'Ankara Strasbourg 67080 CEDEX, France email: saudekv@synbio.tpgnet.net tel: +33 (0)3 8860 8893 fax: +33 (0)3 8845 9070 ********************************************* Vladimir Saudek Structural Biology Synthelabo Biomoleculaire 16, rue d'Ankara Strasbourg 67080 CEDEX, France email: saudekv@synbio.tpgnet.net tel: +33 (0)3 8860 8893 fax: +33 (0)3 8845 9070 PLEASE NOTE THE NEW TELEPHONE NUMBER ********************************************* From owner-7tms_r@net.bio.net Wed Dec 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: newsmgr@merrimack.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: G-proteins/alpha subunits of 293 cell lines Date: 4 Dec 1997 15:12:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <667dch$cbl@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chasm Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-feed3.bbnplanet.com!news.bbnplanet.com!topgun.es.dupont.com!usenet Newsgroups: bionet.molbio.proteins.7tms_r Subject: G-proteins/alpha subunits of 293 cell lines Message-ID: <3486EDE8.73E9@usa.dupont.com> From: romingdh Date: Thu, 04 Dec 1997 12:52:41 -0500 Organization: DuPont Merck Pharmaceutical Company Lines: 9 NNTP-Posting-Host: esm155120.dmpc.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) I have a question: Are there any ref.'s or does anyone know the g-proteins contained in a 293 HEK cell line and or the g_alpha's expressed within? _________________________________________________________ David Rominger DuPont Merck Pharmaceuticals romingdh@usa.dupont.com Phone (302) 695-7142 FAX (302) 695-3730 From owner-7tms_r@net.bio.net Wed Dec 10 22:00:00 1997 Path: biosci!biosci!not-for-mail From: "Ian Murray" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Binding experiments Date: 11 Dec 1997 09:58:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <66p9k2$n6a@net.bio.net> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. - ------=_NextPart_000_0004_01BD05C1.AA959A40 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hello, Forgive me if this is a simple question. I am performing binding of = a radiolabelled ligand to a 7TM receptor. I have performed this on cells = in the absence of calcium. I was wondering if calcium (physiological = levels) is required for binding and if so does it have allosteric = effects of can alter receptor structure. Sincerely, Ian Murray imurra@po-box.mcgill.ca - ------=_NextPart_000_0004_01BD05C1.AA959A40 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hello,
 
    Forgive me if = this is a=20 simple question. I am performing binding of a radiolabelled ligand to a = 7TM=20 receptor. I have performed this on cells in the absence of calcium. I = was=20 wondering if calcium (physiological levels) is required for binding and = if so=20 does it have allosteric effects of can alter receptor = structure.
    = Sincerely,
Ian Murray
imurra@po-box.mcgill.ca
- ------=_NextPart_000_0004_01BD05C1.AA959A40-- From owner-7tms_r@net.bio.net Fri Dec 12 22:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 13 Dec 1997 11:40:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 232 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <66uoc6$klt@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! - ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. - -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. - ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? - --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? - ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. - ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI - ------------------------------------------------------------------ node at computer net.bio.net: - ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at - -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): - ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. - ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Tue Dec 16 22:00:00 1997 Path: biosci!biosci!not-for-mail From: newsmgr@merrimack.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: US-WA-DIRECTOR OF TISSUE AND CELL BIOLOGY Date: 17 Dec 1997 07:36:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <678rh9$k33@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chasm Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!206.214.99.1!ix.netcom.com!news Newsgroups: bionet.molbio.proteins.7tms_r Subject: US-WA-DIRECTOR OF TISSUE AND CELL BIOLOGY Message-ID: <01bd0af3$66c418a0$6d79d6ce@scott-s-armada> From: "Scott Shanes" Date: 17 Dec 1997 13:54:02 GMT Organization: Netcom Lines: 15 NNTP-Posting-Host: trn-nj4-13.ix.netcom.com X-NETCOM-Date: Wed Dec 17 5:54:02 AM PST 1997 X-Newsreader: Microsoft Internet News 4.70.1161 I am looking for a person to be the Director of Tissue and Cell Biology. The candidate will be responsible for inventing and developing improved in vitro models for drug absorption, metabolism, and toxicology. They will also be in charge with developing artificial tissue models. This person will be responsible for recruiting and managing a staff of 10+ scientists. The candidate will be in charge of identifying outside technology licensing opportunities. You should possess a Ph.D., MD or DVM with 5 years relevant experience. Our client is a leading drug discovery company with research facilities near Seattle, Washington and can provide excellent benefits (health insurance, dental, and vision plan, stock options, paid vacation, 401K and more). A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax CV and cover letter to 609-584-9575 or E-Mail to sis@diedrmoire.com From owner-7tms_r@net.bio.net Tue Dec 16 22:00:00 1997 Path: biosci!biosci!not-for-mail From: philchee@accel.net (Phillip Chee) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Ligand binding..computer anlaysis Date: 17 Dec 1997 07:37:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <678ris$k60@net.bio.net> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. - ------=_NextPart_000_000A_01BD0A6C.1F8382A0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hello, =20 I am interested in the molecular recognition theory or antisense = homology boxes analysis of ligand binding to 7TM receptors. How = universal is this type of interaction, as it has been applied to = complement C5a, vasopressin, angiotensin and other ligands. I would appreciate any references or advice on this topic. =20 Sincerely, Ian Murray imurra@po-box.mcgill.ca - ------=_NextPart_000_000A_01BD0A6C.1F8382A0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hello,
 
    I am interested = in the=20 molecular recognition theory or antisense homology boxes analysis of = ligand=20 binding to 7TM receptors. How universal is this type of interaction, as = it has=20 been applied to complement C5a, vasopressin, angiotensin and other=20 ligands.
    I would = appreciate any=20 references or advice on this topic.
 
Sincerely,
Ian Murray
imurra@po-box.mcgill.ca
- ------=_NextPart_000_000A_01BD0A6C.1F8382A0-- From owner-7tms_r@net.bio.net Fri Dec 19 22:00:00 1997 Path: biosci!biosci!not-for-mail From: "Ian Murray" Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCR and ligand blots Date: 20 Dec 1997 13:55:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 90 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <67hetb$fdm@net.bio.net> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. - ------=_NextPart_000_0004_01BD0CA4.331AB4C0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hello, I recently read that 7TM receptors will not survive SDS page gels and = allow for ligand blots (with the exception of glycoprotein receptors = LH,FSH: posting in this news group). Is it possible to perform ligand = blots using 7TM and if so could you provide me with a reference for the = technique.=20 My colleague had performed ligand blots using SDS PAGE run proteins and = I think that the bands may be due to the cationic ligand sticking to = residual SDS on the proteins within the blot. My colleague says that the = SDS is removed during the transfer in SDS free Western transfer solution = by the electrophoretic current and the methanol. Is there any support = for either side? I am performing binding to a receptor. I prefer the crosslinking method = to visualise the reactive receptor on gels rather than SDS PAGE, = westerns (transfer to PVDF using sodium carbonate buffer and Methanol) = followed by ligand blots. Sincerely, Ian Murray imurra@po-box.mcgill.ca - ------=_NextPart_000_0004_01BD0CA4.331AB4C0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hello,
 
I recently read that 7TM receptors = will not=20 survive SDS page gels and allow for ligand blots (with the exception of=20 glycoprotein receptors LH,FSH: posting in this news group). Is it = possible to=20 perform ligand blots using 7TM and if so could you provide me with a = reference=20 for the technique.
 
My colleague had performed ligand = blots using=20 SDS PAGE run proteins and I think that the bands may be due to the = cationic=20 ligand sticking to residual SDS on the proteins within the blot. My = colleague=20 says that the SDS is removed during the transfer in SDS free Western = transfer=20 solution by the electrophoretic current and the methanol. Is there any = support=20 for either side?
 
I am performing binding to a = receptor. I prefer the crosslinking method to visualise the = reactive=20 receptor on gels rather than SDS PAGE, westerns  (transfer to PVDF = using=20 sodium carbonate buffer and Methanol) followed by ligand = blots.
 
Sincerely,
Ian Murray 
imurra@po-box.mcgill.ca
 
 
 
- ------=_NextPart_000_0004_01BD0CA4.331AB4C0-- From owner-7tms_r@net.bio.net Tue Dec 23 22:00:00 1997 Path: biosci!biosci!not-for-mail From: yc3 Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gao expression in Sf21 cells Date: 24 Dec 1997 04:23:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <67quro$qg@net.bio.net> NNTP-Posting-Host: net.bio.net Does anybody have experience in expression of G alpha o in Sf21 or Sf9 cells using the baculovirus system? If so has expression been confirmed with Anti-Go antibody or by functional response? Thanks in advance Yolande Cordeaux From owner-7tms_r@net.bio.net Sun Dec 28 22:00:00 1997 Path: biosci!biosci!not-for-mail From: hla@sun.uchc.edu (Timothy Hla) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BLOOD VESSEL CLUB at Exp. Biol.'98 Meeting in SF Date: 29 Dec 1997 14:40:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6898st$dju@net.bio.net> NNTP-Posting-Host: net.bio.net CALL FOR PRESENTATIONS: The North American Vascular Biology Organization (NAVBO) is sponsoring the BLOOD VESSEL CLUB in the 1998 Experimental Biology '98 meeting in San Francisco, CA. It will be held on Saturday afternoon, April 18, 1998, at the beginning of the annual meeting. The Blood Vessel Club (BVC) was founded in 1973 by Ted Spaet and Ramzi Cotran. The idea was to have a very informal setting devoted to discussion of late breaking discoveries in the then new field of vascular biology. In the continuation of this tradition the BVC is convened each year with the aim of creating a truly informal, interactive and exciting atmosphere for the BVC. The program will be composed of 2-3 short presentations (20-25 min each) and 5-6 "Blitzes" (5-10 min each) and will be selected from cutting-edge, late-breaking and exciting areas of vascular biology. Ample discussion time will be available for these presentations. The program will be announced widely prior to the meeting and also via the WWW and e-mail to the vascular biology community and other attendees. Please submit a short abstract or contact: Timothy Hla, PhD Department of Physiology, MC-3505 University of Connecticut School of Medicine 263 Farmington Avenue Farmington, CT 06030 phone: 860-679-4128 fax: 860-679-1269 email: hla@sun.uchc.edu From owner-7tms_r@net.bio.net Sun Dec 28 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Webmaster@scienceguide.com (Robert W. Georgantas III) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Science News Date: 29 Dec 1997 14:40:29 -0800 Organization: The Science Guide Lines: 258 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6898st$dkj@net.bio.net> Reply-To: news@scienceguide.com NNTP-Posting-Host: net.bio.net Sign up for the SCIENCE GUIDE DAILY NEWS UPDATE. This free daily newsletter summerizes science related news from news and academic institutions from around the world. To receive the News Update simply send and email to news@scienceguide.com with "subscribe" in the subject field. An example of the News Update from Dec 29th follows: THE SCIENCE GUIDE DAILY NEWS UPDATE The Science News from 10:59 AM EST; Monday, December 29, 1997 You have two ways to use the Science Guide News Update: 1) Goto our News Page (http://www.scienceguide.com/News/News_LowGraph.html) 2) If your email program supports web links you can simply click on the URL listed below each story title, or you can simply copy the URL into you browser's location bar\ ******** Features From Daily News Sources ******* BIOLOGY, BIOTECHNOLOGY, AND MEDICINE **** Birds slaughtered in bid to contain mystery flu - CNN Heath http://www.scienceguide.com/News/News_Articles/122997Article_6.html Poultry workers and government teams began the wholesale slaughter of birds Monday, the first step in a plan to contain the mysterious "bird flu" virus that has crossed over to humans and caused four recent deaths. **** Mystery disease in Kenya and Somalia could be form of anthrax - CNN Heath http://www.scienceguide.com/News/News_Articles/122997Article_7.html An unexplained disease that has caused scores of Kenyans, Somalis and livestock to bleed to death this month may be a form of anthrax, medical experts said Sunday. **** Oral Test for HIV Virus Is Winning Converts - New York Times http://www.scienceguide.com/News/News_Articles/122997Article_14.html The oral test, made by Epitope Inc. of Beaverton, Ore., isn't likely to replace the more traditional roll-up-your-sleeve test any time soon, because millions of HIV tests are done each year on blood donations or in conjunction with other blood-based medical tests. But OraSure _ just one of several alternative HIV tests on the market, including a home blood test, a blood test with a 10-minute response test and a urine test _ is winning converts from coast to coast because it's so easy to take, and virtually as accurate as a blood test. **** Nutrition Questions and Answers - New York Times http://www.scienceguide.com/News/News_Articles/122997Article_15.html **** Comment: Consumer-Rights Bill Doesn't Help Doctors - New York Times http://www.scienceguide.com/News/News_Articles/122997Article_16.html Anytime you give something away for nothing, you will never have enough of it. That is what is happening to physicians' time under managed care. We are not only expected to give it away, patients are being given the right to it. **** Forbes reports that Tylenol can be dangerous in big doses - Nando Times http://www.scienceguide.com/News/News_Articles/122997Article_18.html Tylenol, safe in proper doses, can be very dangerous in slightly larger doses, Forbes magazine reported in its latest issue. **** In the fight against AIDS, some find reason to hope - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_21.html With new drug regimens fueling a decline in AIDS mortality in the United States, Americans in 1997 were more optimistic than ever about the possibility of conquering the devastating illness. But surveys show that the public, far from thinking the epidemic is over, feels that the widespread emergence of breakthrough therapies this year has not signaled the end of AIDS. **** AIDS drugs - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_22.html Eight months after Vice President Al Gore asked for a policy change allowing poor people with HIV to get effective new drugs for free, the government has concluded the plan is too costly. **** Innovation - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_25.html Biotech firm develops cancer vaccines. **** The DNA Drugstore: How Genetics is Changing our Lives: Part Six - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_26.html PHYSICS, ASTRONOMY & ASTROPHYSICS, SPACE, AERONAUTICS, AND AEROSPACE **** After bad start, Galileo is sending back dramatic data about Jupiter - - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_23.html NASA's Galileo mission to Jupiter threatened at times in its long journey to become a humiliating failure, slowly playing out its sad fate beyond help, almost half a billion miles from the sun. Instead, the spacecraft overcame a crippled main antenna and other problems, and its human handlers have successfully completed their two-year prime mission, producing dramatic evidence that the Jovian ice-moon Europa once harbored a liquid ocean capable of nourishing life -- and may still. **** NASA's Prospector to search for water on moon - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_24.html Twenty-five years after men last roamed the moon, NASA is returning with a little robot named Prospector. Diminutive as it is and cheap, too, NASA's 4-foot Lunar Prospector will search for something more precious to scientists than gold: evidence of frozen water at the moon's poles. The spacecraft also will rummage for gases and minerals that, like polar ice, could be used by human settlers. AGRICULTURE **** Report: U.S. to slash limits on Pacific Coast fishing - CNN Earth http://www.scienceguide.com/News/News_Articles/122997Article_8.html Fearing a sharp decline in Pacific fish, the federal government will toughen restrictions on the commercial catch for 83 species, including popular black cod, ling cod, dover sole and rock fish, the Los Angeles Times reported Sunday. **** Scientists using Boston Harbor to farm fish - CNN Earth http://www.scienceguide.com/News/News_Articles/122997Article_11.html There was a time when all Boston Harbor seemed able to produce was stink and slime. Now they want to raise fish there. **** 'Bird flu' scare hits Chinese farmers - Nando Times http://www.scienceguide.com/News/News_Articles/122997Article_17.html A ban on chicken exports from mainland China to Hong Kong prompted by the "bird flu" scare is devastating farmers in southern Guangdong province, a Chinese official said Monday. ENGINEERING, ENERGY, MATHEMATICS, ANDTECHNOLOGY **** Pentagon introducing high-tech dog tags - CNN Sci-Tech http://www.scienceguide.com/News/News_Articles/122997Article_1.html By 1999, the 1.4 million members of the active duty military will be issued a high-tech dog tag that contains a computer chip. **** Is wind the energy future for Texas? - CNN Sci-Tech http://www.scienceguide.com/News/News_Articles/122997Article_4.html Texas and oil are in many ways synonymous, and pumping stations have been a familiar part of the region's landscape for decades. But there may soon be other structures springing up in the state: there is a push to use wind energy for commercial purposes and to set up the kind of fields of wind turbines already familiar elsewhere. **** 'Smart gels' improve eyedrops, muscles, in-line skates - CNN Sci-Tech http://www.scienceguide.com/News/News_Articles/122997Article_5.html They look like floating Christmas tree ornaments made of jello, bobbing in a small aquarium. But he gel-like balls could prove instrumental in helping treat wounds or improve eyedrops. CHEMISTRY. COMPUTERS, COMPUTER SCIENCE, AND THE INTERNET **** Army tests virtual systems for soldier training - CNN Sci-Tech http://www.scienceguide.com/News/News_Articles/122997Article_2.html While it's still effective to send infantry soldiers crawling through the mud during basic training, the U.S. Army is testing four virtual-reality systems that can simulate battlefields and teach specialized skills. **** Remote-control system uses brain waves - CNN Sci-Tech http://www.scienceguide.com/News/News_Articles/122997Article_3.html Something spooky's afoot. Lights turn on without warning. The TV flashes channels at random. In the distance, a chime goes off. But there are no poltergeists here. ANTHROPOLOGY, ARCHAEOLOGY, LINQUISTICS, AND PALEONTOLOGY ENVIRONMENTAL SCIENCES & ISSUES, GEOLOGY, METEOROLOGY, AND OCEANOGRAPHY **** Study shows environmental risks of animal waste - EurekAlert http://www.scienceguide.com/News/News_Articles/122997Article_9.html The staggering amount of animal waste produced on American farms often pollutes water, and the risk is growing as more large-scale livestock operations take hold, according to a new U.S. Senate study. **** Egypt envisions future beyond the Nile - CNN Earth http://www.scienceguide.com/News/News_Articles/122997Article_10.html A platoon of bulldozers plows methodically in the parched, unforgiving dirt, waging a dust-choked mission across a sea of golden sand broken by black cliffs and desert brush. **** EPA makes record number of fines, referrals in 1997 - CNN Earth http://www.scienceguide.com/News/News_Articles/122997Article_12.html The Environmental Protection Agency says it referred a record number of cases for prosecution and assessed its largest total of fines in 1997. **** Large amounts of animal manure pose environmental risks, study shows - - Nando Times http://www.scienceguide.com/News/News_Articles/122997Article_19.html The huge amount of animal waste produced on American farms often pollutes water, and the risk is growing as more large-scale livestock operations take hold, according to a new U.S. Senate study **** Has the world reached its 'Limits to Growth'? - Nando Times http://www.scienceguide.com/News/News_Articles/122997Article_20.html Our blood ran cold as we sat in the dark that year, millions of moviegoers chilled by the vision of "A Clockwork Orange," of a "horrorshow" future of anarchy and violence. **** Growing concerns about persistent organic pollutants - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_27.html Massive oil spills, fires at chemical plants and leaking radioactive waste, because of the highly toxic materials involved, rarely fail to attract widespread attention. Much less is known, however, about the environmental and health effects of other chemicals that, while less toxic, are much more widely distributed. **** When's the air really dirty? - Excite.com News http://www.scienceguide.com/News/News_Articles/122997Article_28.html How can Denver's air be so good and yet look so bad? The air quality is undeniably better than it used to be. Just 25 years ago the metro area exceeded federal air standards 125 times a year. And on almost every winter day, the pollution was at levels harmful to public health. PSYCHOLOGY **** Stepchildren May Expand Pool Of Caregivers For Baby Boomers - EurekAlert http://www.scienceguide.com/News/News_Articles/122997Article_13.html Unprecedented rates of divorce and remarriage are already redefining families of baby boomers. According to a new analysis from University of California, Berkeley, when the boomers become the elderly of the future, they will have impressive numbers of stepchildren and stepgrandchildren, expanding the numbers of family members on whom they may rely, if necessary. - -- Robert Georgantas BS, MS, PhD (candidate) Researcher in Experimental Immunotheraputics Department of Pharmacology and Molecular Sciences The Johns Hopkins University School of Medicine Also publisher of: The Science Guide The World Wide Web News and Information Service for Scientists http://www.scienceguide.com From owner-7tms_r@net.bio.net Tue Dec 30 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Michael Levine Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post-Doctoral Position Available Date: 31 Dec 1997 08:56:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <68dtf8$lrk@net.bio.net> NNTP-Posting-Host: net.bio.net
Post-Doctoral Position Available
An NIH-sponsored (U.S. citizen or permanent resident) postdoctoral position is available immediately to work with an active group of scientists in the Molecular Endocrinology Laboratory at Johns Hopkins in research on G protein coupled signal transduction and hormone action. A strong background in molecular and cellular biology is required. Expertise in gene cloning and expression, differential gene expression analysis, and transgenic animal development is highly desirable. Applicants should send or FAX a description of their experience, a curriculum vitae including a bibliography, and three letters of reference to: Dr. Michael A. Levine, Division of Endocrinology, The Johns Hopkins School of Medicine, 863 Ross Research Building, 720 Rutland Avenue, Baltimore, MD 21205-2109; FAX: 410.955.0841. E-mail: mlevine@jhu.edu. Michael A. Levine, M.D. Johns Hopkins University School of Medicine Division of Endocrinology and Metabolism 863 Ross Research Building 720 Rutland Avenue Baltimore, MD 21205 T 410.955.7233 F 410.955.0841 ------- End of forwarded message ------- From owner-7tms_r@net.bio.net Tue Dec 30 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Henrik Dohlman Newsgroups: bionet.molbio.proteins.7tms_r Subject: G protein workshop - May 1 Date: 31 Dec 1997 10:05:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <68e1ge$rlr@net.bio.net> NNTP-Posting-Host: net.bio.net The first annual "Tri-state G protein Signaling Workshop," will take place on May 1, 1998, at Mt. Sinai Medical Center in New York City. This day long symposium will feature talks and posters by senior graduate students, post-docs, and young investigators from the NY/NJ/CT area. Paul Sigler and Robert Lefkowitz have agreed to present keynote seminars. I hope that you and your colleagues will plan to attend, and present a talk or poster. There is no charge to attend. SPACE IS LIMITED - IF YOU PLAN TO ATTEND, PLEASE REGISTER IMMEDIATELY online at: http://www.med.yale.edu/gprotein/ I look forward to seeing you in New York this Spring! Henrik G. Dohlman, PhD Assistant Professor Department of Pharmacology Yale University, School of Medicine henrik.dohlman@yale.edu 203-737-2203 (-2290 FAX)