From owner-7tms_r@net.bio.net Sun Mar 01 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Menoret Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: stable expression of GPCRs Date: 2 Mar 1998 15:38:53 -0800 Organization: @Home Network Lines: 23 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6dffud$84k@net.bio.net> References: <6d76la$4kr@net.bio.net> Reply-To: menoret@home.com NNTP-Posting-Host: net.bio.net Hi John, I am extremly interested in the ecdysone system, all the information and suggestions will be highly appreciated. I think SIGMA sell the muristerone A : Product #M7888 Found on Page: 736 of the Catalog MURISTERONE A Sigma Catalog Date: Jan 1 1997 Packaging: Sz/Qty US wgt Frgn wgt 1 MG .1 .1 5 MG .1 .1 10 MG .1 .1 25 MG .1 .1 Steroids CAS NUMBER: 038778-30-2 Research Non-Hazardous: Mailable Normally Shipped by Air Store at < 0 degree Centigrade Antoine Menoret menoret@home.com menoret@up.uchc.edu From owner-7tms_r@net.bio.net Sun Mar 01 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Menoret Newsgroups: bionet.molbio.proteins.7tms_r Subject: HSC70 gene Date: 2 Mar 1998 15:39:06 -0800 Organization: @Home Network Lines: 15 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6dffuq$85d@net.bio.net> Reply-To: menoret@home.com NNTP-Posting-Host: net.bio.net Hi all, I am desperately looking for the complete cDNA or genomic DNA of the mouse HSC70. Any help is greatly appreciated. Please e-mail your response, as I only rarely have time for newsgroups. Thanks in advance !! Antoine Menoret menoret@home.com menoret@up.uchc.edu tel : (1) 860 679 4444 fax : (1) 860 679 4365 From owner-7tms_r@net.bio.net Tue Mar 03 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Jonathan Lee Newsgroups: bionet.molbio.proteins.7tms_r Subject: Ph.D. Position Open: Cell Biologist/Molecular Pharmacologist Date: 4 Mar 1998 05:59:44 -0800 Organization: Eli Lilly and Company Lines: 40 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6djmog$a8q@net.bio.net> NNTP-Posting-Host: net.bio.net Ph.D. Molecular Pharmacologist/Cell Biologist: Lilly is interested in identifying a molecular pharmacologist or cell biologist to join an ongoing effort to develop and utilize fluorescence-based methods for the functional assay of receptors, channels, and transporters. SCIENTIFIC BACKGROUND: The candidate should have a Ph.D., post doctoral experience in biochemistry, molecular biology, pharmacology, cell biology or a related field with demonstrated expertise in signal transduction and integral membrane protein biochemistry (cytokine receptors, channels, G-protein coupled receptors and/or transporters). The ideal candidate will be proficient in technologies related to molecular biology (PCR, DNA preparation and subcloning), mammalian cell expression, receptor biochemistry, and signal transduction (assays for Ca, inositol phosphate, intracellular pH, reporter genes). In addition, experience in fluorescence photometry, confocal microscopy, configuration of high through-put functional assays, and laboratory robotics would be an advantage. GENERAL ATTRIBUTES: Enthusiastic and self motivated individual with excellent communication skills with interests in (1) developing fluorescence assays to monitor changes in cellular metabolism and/or signal transduction, (2) genomic database mining for novel targets and/or agents, and (3) initiating and championing novel drug discovery and/or assay development projects. For consideration contact: Dr. Jonathan A. Lee Research Technologies and Protein Mail Stop 1533 Eli Lilly and Company Lilly Corporate Center Indianapolis, Indiana 46285 Phone: 317-277-8123 Fax: 317-276-5281 EMAIL: Jonathan_A_Lee@lilly.com From owner-7tms_r@net.bio.net Wed Mar 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Steve Qi Newsgroups: bionet.molbio.proteins.7tms_r Subject: reporter gene for IP3 Date: 5 Mar 1998 06:05:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6dmbeq$4hs@net.bio.net> NNTP-Posting-Host: net.bio.net Dear fellow colleagues, I am currently trying to construct a reporter gene cell line for monitoring the IP3/DAG signal pathway. I have cloned the ICAM-1 promoter and inserted the 5' untranslated region (-616 to -51) as a XhoI - SalI fragment into pGL3basic (Promega). The construct response to PdBU (5 - 7X) but has very high basal activity. Can anybody give me some suggestions on how to reduce the background or suggest another response element? Thanks Steve (sqi@ferring.demon.co.uk) From owner-7tms_r@net.bio.net Wed Mar 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Paul Eggleton Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Calreticulin monoclonals Date: 5 Mar 1998 06:05:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6dmbfr$4r9@net.bio.net> NNTP-Posting-Host: net.bio.net Dear All, Does anyone know of, or have a monoclonal antibody against correctly folded human calreticulin (i.e an antibody which can tell the difference between correctly folded and incorrectly folded protein). If you do please contact Steve Johnson on email: johnson@bioch.ox.ac.uk here in our lab in Oxford. Many thanks Paul Eggleton. ------- End of forwarded message ------- From owner-7tms_r@net.bio.net Fri Mar 06 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Philip Aisen Newsgroups: bionet.molbio.proteins.7tms_r Subject: Drosophila expression system Date: 7 Mar 1998 08:53:21 -0800 Organization: Albert Einstein College of Nedicine Lines: 7 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6dru21$go1@net.bio.net> NNTP-Posting-Host: net.bio.net If anyone has had experience with the Invitrogen Drosophila expression system for inducible, secreted expression I would be grateful for comments. Thanks. Philip Aisen From owner-7tms_r@net.bio.net Thu Mar 12 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 13 Mar 1998 09:36:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 232 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6ebqqu$hlm@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! - ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. - -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. - ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? - --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? - ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. - ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI - ------------------------------------------------------------------ node at computer net.bio.net: - ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at - -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): - ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. - ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Thu Mar 12 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Yves Durocher Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post doc position in GPCR signalling Date: 13 Mar 1998 09:36:49 -0800 Organization: National Research Council Canada Lines: 23 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6ebqrh$hmb@net.bio.net> Reply-To: yves.durocher@nrc.ca NNTP-Posting-Host: net.bio.net A postdoctoral position is available in the Bioprocess Sector of the Biotechnology Research Institute. The research involves G Protein-Coupled Receptors (GPCR) signal transduction characterisation in mammalian cell transfectomas and is part of a collaborative project involving a major industrial partner. The work is to take place at the Biotechnology Research Institute, part of the National Research Council of Canada and located in Montreal (Quebec). The candidate must have strong experience in molecular biology, biochemistry and mammalian cell culture. Knowledge in GPCRs pharmacology is an asset. The modern, well-equipped laboratories are supported by extensive core facilities, including DNA synthesis and sequencing, peptide synthesis and sequencing, an 850 mHz NMR, a confocal microscopy facility, and a large Bioprocess sector with 3 to 1500L bioreactors. A number of the research labs at the Institute focus on diverse aspects of cellular signaling mediated by G protein-coupled receptors, receptor tyrosine kinase and tyrosine phosphatases, and mechanisms of apoptosis, offering a stimulating intellectual environment. Starting date: ASAP Contact Person: Yves Durocher Phone: (514) 496-6192 FAX: (514) 496-6785 Email: yves.durocher@nrc.ca From owner-7tms_r@net.bio.net Mon Mar 16 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Paul Ernsberger, Ph.D." Newsgroups: bionet.molbio.proteins.7tms_r Subject: Catecholamine Club meeting Date: 17 Mar 1998 03:55:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6eloal$oto@net.bio.net> NNTP-Posting-Host: net.bio.net All researchers with an interest in the catecholamines and their receptors who are attending the Experimental Biology meeting in San Francisco should come to the Catecholamine Club Dinner Meeting, which will take place Tuesday, April 21, 1998 at the Empress of China restaurant in San Francisco. The dinner speaker will be Sydney Spector from the Psychiatry and Pharmacology departments at Vanderbilt, who will speak on "Forty years after the First International Catecholamine Symposium". Dr. Spector will receive the Julius Axelrod-SmithKline Beecham Medal for outstanding scholarship in catecholamine research. Tickets to this event are available only by mail from the club treasurer: Catecholamine Club Dr. Walter R. Dixon Department of Pharmacology and Toxicology University of Kansas School of Pharmacy Lawrence, KS 66045-2500 Send a check for $35 for the dinner, or $40 for dinner and membership dues to the Catecholamine Club. /\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Paul Ernsberger, Ph.D., Department of Nutrition, Case Western Reserve University School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4906 Web address: http://www.cwru.edu/med/nutrition/ernsberger.htm Email: pre@po.cwru.edu; FAX: (216) 368-4752 From owner-7tms_r@net.bio.net Wed Mar 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Richard Alper Newsgroups: bionet.molbio.proteins.7tms_r Subject: 5-CT Date: 19 Mar 1998 10:14:30 -0800 Organization: The University of Kansas School of Medicine Lines: 10 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6erna6$7bk@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking to find out if anyone knows whether the serotonin agonist 5-CT has any efficacy at beta-adrenoceptors. If you have any infomtion, please let me know. Thanks Rich Alper Department of Pharmacology University of Kansas School of Medicine ralper@kumc.edu