Here is an example .ace file with restriction enzyme objects that work,
at least here:
// data dumped from keyset display
Motif : "EcoRI"
Match_sequence "GAATTC"
Offset 1
Cleavage "G'AATT_C"
Overhang 4
Company "Amersham"
DNA
Motif : "EcoRII"
Match_sequence "CCwGG"
Isoschizomer "ApyI"
Isoschizomer "BstNI"
Isoschizomer "MvaI"
Isoschizomer "TspAI"
Offset 0
Cleavage "'CCwGG_"
Overhang 5
Company "BRL"
DNA
Motif : "EcoRV"
Match_sequence "GATATC"
Isoschizomer "Eco32I"
Offset 3
Cleavage "GAT'ATC"
Overhang 0
Company "Amersham"
DNA
The whole file we made about 7 years ago from rebase is in
ftp://ftp.sanger.ac.uk/pub/acedb/useful/restrict.ace
Richard
