Lab database: new user questions

Richard Durbin rd at sanger.ac.uk
Thu Oct 29 12:58:51 EST 1998


Here is an example .ace file with restriction enzyme objects that work,
at least here:

// data dumped from keyset display

Motif : "EcoRI"
Match_sequence	 "GAATTC"
Offset	 1
Cleavage	 "G'AATT_C"
Overhang	 4
Company	 "Amersham"
DNA	

Motif : "EcoRII"
Match_sequence	 "CCwGG"
Isoschizomer	 "ApyI"
Isoschizomer	 "BstNI"
Isoschizomer	 "MvaI"
Isoschizomer	 "TspAI"
Offset	 0
Cleavage	 "'CCwGG_"
Overhang	 5
Company	 "BRL"
DNA	

Motif : "EcoRV"
Match_sequence	 "GATATC"
Isoschizomer	 "Eco32I"
Offset	 3
Cleavage	 "GAT'ATC"
Overhang	 0
Company	 "Amersham"
DNA	


The whole file we made about 7 years ago from rebase is in 

ftp://ftp.sanger.ac.uk/pub/acedb/useful/restrict.ace

Richard




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