DNA preparation from birch

S A Rolfe S.Rolfe at shef.ac.uk
Tue Oct 28 12:10:22 EST 1997


Dear all,

Does anyone have a good method for extracting DNA from young birch 
seedlings for use in Southern blots?  We have tried a variety of methods 
(including CTAB, phenol extractions etc) but have problems with extremely 
viscous supernatants (even when the plant:extraction volume ratio is 
low).  Any help would be much appreciated.

Thanks.
Steve Rolfe
University of Sheffield, UK
(s.rolfe at sheffield.ac.uk)



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