DNA Sequencers for Sale

Eric Rappaport rappaport at email.chop.edu
Mon Feb 23 18:12:52 EST 1998


AUTOMATED DNA SEQUENCERS FOR SALE

   Three ABI 373 DNA sequencers are available for sale.  Please contact
Dr. Eric Rappaport (rappaport at email.chop.edu) with offers or questions. 
Details of the 
equipment configuration follow:
   
Two 373 units with Stretch upgrades:   

Filters - 5-filter wheel (can choose between 2 different sets of 4
different dyes).

Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 DNA
polymerase         sequencing chemistry.  All current Genescan
chemistries, including         microsatellite panels, primers labeled
during synthesis with standard ABI        dyes (6-Fam, Hex, Tet, Tamra) or
(6-Fam, Hex, Tamra, and Rox). 

Gel lengths - Multiple gel (well-to-read) lengths including 48 cm
(standard long-read [>650 nt] sequencing length), 34 (shorter sequence
reads [550-600 nt]          or high-resolution Genescan applications), 24
(microsatellite applications)         12 (lower-resolution Genescan
applications) or 6 (lower-resolution            Genescan applications).

Scan Speed - 1X, BaseSprinter (2X, but limited to middle half of gel; can
be used for faster analysis of shorter sequences).

   
One 373 with multiple gel length (Leon) configuration:   

Filters - 5-filter wheel (can choose between 2 different sets of 4
different dyes).

Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 DNA
polymerase         sequencing chemistry.  All current Genescan
chemistries, including         microsatellite panels, primers labeled
during synthesis with standard ABI        dyes (6-Fam, Hex, Tet, Tamra) or
(6-Fam, Hex, Tamra, and Rox). 

Gel lengths - Multiple gel (well-to-read) lengths including 24 cm (shorter
sequence reads [400-500 nt] or microsatellite applications) 12
(lower-resolution          Genescan applications) or 6 (lower-resolution
Genescan applications).

Scan Speed - 1X, BaseSprinter (2X, but limited to middle half of gel; can
be used for faster analysis of shorter sequences).
   

Computers and Software

   Unit 1 (Stretch) - Mac IIcx with 8 mB RAM/230 mB hard drive
   Unit 2 (Stretch) - Mac IIci with 8 mB RAM/100 mB hard drive
   Unit 3 (Leon) - Mac IIci with 8 mB RAM/120 mB hard drive

   All units are equipped with latest versions of analysis and collection
sofware for running sequencing and Genescan applications on the above
computers.  Upgrades for PowerMac are not included.

Service Contracts

   All units have been under service contract since the time of their
purchase from ABI and have onlt recently been removed from those
agreements.  Purchaser would need to have an installation service call
from ABI before initiation of a new service contract could occur.

Shipping

   Purchaser is responsible for all shipping charges.  ABI will sell
shipping crates which are appropriate for the units, if necessary.



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