determination of apoptosis

Inst. f. Biochemie dh7y at .pop.th-darmstadt.de
Fri Jun 10 02:23:30 EST 1994


Hello World,

in our lab we are starting to investigate apoptosis in general with special regards to endothelial cells.
As we read many papers we are confused, because some of the investigators use the phenol/chloroform extraction before loading the DNA on the agarose gel and some investigators don´t extract with phenol.
My question is, which method is better or more recommendable?

Hoping to get a lot of valuable informations

Gangolf

Gangolf Schrimpf
Institut fuer Biochemie
Petersenstrasse 22
D-64287 Darmstadt
Tel.: 06151/165157
e-mail: dh7y at pop.th-darmstadt.de







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