Hayflick limit for rapid dividing cells (fwd)

[Kingsley G. Morse Jr.]

In <Pine.3.05.9410131835.A13322-c100000 at essex.hsc.colorado.edu> kruged at ESSEX.HSC.COLORADO.EDU (Edward Krug) writes:

>	I currently grow human foreskin fibroblasts in culture as host for
>an intracellular parasite.  I find that with high passage numbers the
                                                   ^^^^^^^^^^^^^^^
What are "passage numbers"?

>fibroblasts become poor hosts for the parasite long before there is a
>noticable decrease in fibroblasts growth rate.  I study different media
>formulations and I have yet to find anything which overcomes sheer passage
>number.