Telomerase - - amino acid composition???
patrick at corona
Sun Apr 16 17:36:49 EST 1995
On 16 Apr 1995, Mark Gardner wrote:
> H.Kugler at ix.netcom.com (Dr. Hans J. Kugler) wrote:
> > We did a lit search to find the amino acid composition (not sequence)
> > of telomerase) and couldn't find anything.
> > Has this been done? If yes, anybody have a reference?
> > Hans J. Kugler, PhD
> > Editor, PREVENTIVE MEDICINE UP-DATE
> There is quite a lot at this address
What you will find is that there are many papers dealing with telomere
structure, function, and replication, but that in spite of all the
research, no protein component of any telomerase has yet been able to be
identified. What HAS been found is the RNA template portion of the
holoenzyme both in Tetrahymena and very recently, in S. cerevisiae
(called TLC1: Gottschling, D. E. and Singer, M. S. 1994. TLC1: template
RNA component of Saccharomyces cerevisiae telomerase. Science 266:404-409).
I have here a HUGE PILE of papers dealing with telomeres from various
aspects as a result of a research proposal I needed to produce. A few of
1. Zakian, V. A. and Schulz, V. P. 1994. The saccharomyces PIF1 DNA
helicase inhibits telomere elongation and de novo telomere formation.
2. Greider, C. W. and Autexier, C. 1994. Functional reconstitution of
wild-type and mutant Tetrahymena telomerase. Genes & Dev. 8:563-575.
3. Gasser, S. M. and Palladino, F. 1994. Telomere maintenance and gene
repression: a common end? Curr. Opin. Cell Biol. 6:373-379.
4. Greider, C. W. 1994. Mammalian telomere dynamics: healing,
fragmentation, shortening and stabilization. Curr. Opin. Gene. Dev.
5. Greider C. W. and Mantell, L. L. 1994. Telomerase activity in
germline and embryonic cells of Xenopus. EMBO J. 13(13):3211-3217.
6. Shore, D. 1994. RAP1: a protean regulator in yeast. TIG
10(11):408-412 **(you will find that in yeast, where many of the genes
that affect telomeres are characterized, that RAP1 and its partners play
a major part in several activities, including telomere regulation...it is
These are a good start, certainly, and they will point you to many more.
Essentially, telomerase activity can be detected but none of the direct
players other than the template for Tetrahymena and yeast telomerase are
known for certain. It makes certain proposals for research rather
difficult to develop without compasses to point out a direction...
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