Epithalamin and longevity in C. elegans

Vladimir V. Bakaev vlad at bakaev.nsk.ru
Sat Mar 14 07:38:40 EST 1998


>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 57.

            AN ATTEMPT TO SLOW AGING IN C. elegans.
             9. NO POSITIVE EFFECT OF EPITHALAMIN
   Vladimir V. Bakaev (e-mail: Vlad at bakaev.nsk.ru), Anatolii V.
Efremov,  Vladimir  N.  Anisimov*  P.O.  Box  45,  Novosibirsk,
630107,  Russia *N.N.Petrov  Research  Institute  of  Oncology,
St.Petersburg, 189646, Russia

There are  data on  the life  span prolonging  effect of pineal
petide  preparation  epithalamin  in  mice, rats and Drosophila
melanogaster  [1,2].
The purpose of this study was to investigate the effect of dif-
ferent concentrations of epithalamin in water solutions on  ne-
matode  life  span.  In this experiment epithalamin was used in
following  dilutions  :  1:10(5),  1:10(6),  1:10(7),  1:10(8),
1:10(9) and 1:10(10). Three adult animals (3 - 5 days old) were
kept in microtitre wells containing 0,75 ml  of  liquid  medium
(with  E.  coli  and without epithalamin) during 4 hours,  then
they were discarded and newborn larvae were transferred in next
wells  (with  epitalamin  in  any concentration) every day (one
worm in one well). This investigation was carried out in tempe-
rature  +21o  C  and in the darkness.  The obtained results are
presented in the following table.
                                                      Table I
‚€€€€€€€€€€€€€€€€€€€€€€€ˆ€€€€€ˆ€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ƒ
    Concentration of              Longevity (days)        
                            †€€€€€€€€€€€€€€ˆ€€€€€€€€€€€€€€€‡
      epithalamin        n    mean+/-S.E.      maximal   
†€€€€€€€€€€€€€€€€€€€€€€€Š€€€€€Š€€€€€€€€€€€€€€Š€€€€€€€€€€€€€€€‡
       Control          12    17,4+/-2,5         29      
        1:10(5)         12    12,8+/-2,1         27      
        1:10(6)         12    15,1+/-1,9         28      
        1:10(7)         12    17,1+/-2,2         28      
        1:10(8)         12    19,6+/-2,2         29      
        1:10(9)         12    18,8+/-2,0         31      
        1:10(10)        12    18,7+/-1,7         25      
„€€€€€€€€€€€€€€€€€€€€€€€‰€€€€€‰€€€€€€€€€€€€€€‰€€€€€€€€€€€€€€€…
                                                      Table II
‚€€€€€€€€€€€€€€€€€€€€€€€ˆ€€€€€ˆ€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ƒ
    Concentration of              Longevity (days)        
                            †€€€€€€€€€€€€€€ˆ€€€€€€€€€€€€€€€‡
      epithalamin        n    mean+/-S.E.      maximal   
†€€€€€€€€€€€€€€€€€€€€€€€Š€€€€€Š€€€€€€€€€€€€€€Š€€€€€€€€€€€€€€€‡
       Control          12    23,5+/-2,3         33      
        1:10(7,5)       12    16,8+/-2,5         34      
        1:10(8,0)       12    19,3+/-1,7         33      
        1:10(8,5)       12    13,7+/-1,4         21      
„€€€€€€€€€€€€€€€€€€€€€€€‰€€€€€‰€€€€€€€€€€€€€€‰€€€€€€€€€€€€€€€…
Conclusion: If the water solution of epithalamin was applied to
C. elegans during the whole life span in above described condi-
tions,  it was not able to prolong the life span of these  ani-
mals.
€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€-
1. Anisimov V.N., Khavinson V.Kh., Morozov V.G. Twenty years of
    study on effects of pineal peptide preparation: Epithalamin
    in experimental gerontology and oncology.
    Ann.N.Y.Acad. Sci., 1994, 719: 483-493.
2. Anisimov V.N., Mylnikov S.V., Oparina T.I., Khavinson V.Kh.
   Effect of melatonin and pinela peptide preparation epithala-
   min  on  life  span and free radical oxidation in Drosophila
   melanogaster. Mech. Ageing Dev., 1997, 97: 81-91.




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