In article <6t1nf8$ks1$1 at birch.prod.itd.earthlink.net>,
excelife at earthlink.net (Excelife) wrote:
>> Yes. as J. Sedivy correctly pointed out in his paper, Proc Natl Acad Sci U S
> A 1998 Aug 4;95(16):9078-9081, mouse cells seem to require only a one step
> process for immortalization as opposed to a two step process for human cells.
What are those steps? I don't know a whole lot about cell-culture techniques!
I'm currently working with several human cancer cell lines to characterise a
new potential gene therapy, and it'd be nice to understand a bit more about
their creation. I got 4 days training before the previous worker left for
another job. They call it the deep end :-/
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